ORF » Species Summary » Schizosaccharomyces pombe » dfr1 cDNA ORF clone

dfr1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following dfr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dfr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99429 NM_001023344.2
Latest version!		 Schizosaccharomyces pombe dihydrofolate reductase Dfr1 (dfr1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    dfr1 ( NM_001023344.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588353.1 Schizosaccharomyces pombe dihydrofolate reductase Dfr1 (dfr1), mRNA.
    Price & Availability↑
    CloneID OSc99429
    Clone ID Related Accession (Same CDS sequence) NM_001023344.2
    Accession Version NM_001023344.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product dihydrofolate reductase Dfr1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023344.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTAAGC CTTTAAAAGT GCTGTGTCTT CATGGATGGA TTCAATCTGG TCCCGTATTT 
    TCAAAAAAAA TGGGATCCGT CCAGAAATAC CTGTCTAAGT ATGCCGAATT GCATTTTCCA 
    ACAGGTCCTG TGGTCGCTGA CGAAGAAGCA GACCCCAATG ATGAAGAAGA GAAGAAACGC 
    CTTGCTGCGC TTGGTGGTGA ACAAAACGGT GGTAAATTTG GGTGGTTTGA AGTGGAGGAT 
    TTTAAAAATA CATACGGAAG TTGGGATGAA AGTCTTGAAT GTATAAATCA ATACATGCAA 
    GAAAAAGGTC CCTTTGATGG ACTTATCGGA TTTTCTCAAG GTGCAGGAAT AGGTGCTATG 
    CTGGCTCAAA TGCTTCAGCC TGGTCAACCT CCCAACCCTT ATGTCCAACA TCCTCCTTTT 
    AAATTTGTTG TTTTTGTTGG CGGTTTCCGT GCTGAAAAGC CTGAATTCGA TCATTTTTAT 
    AACCCCAAGC TTACCACTCC TTCGTTACAT ATTGCTGGTA CTTCTGATAC CCTGGTCCCA 
    CTTGCTCGCT CCAAACAGTT GGTTGAACGT TGCGAAAATG CACACGTTTT GTTGCATCCG 
    GGACAACATA TTGTTCCTCA ACAAGCAGTG TATAAGACTG GCATCCGTGA TTTCATGTTT 
    TCCGCCCCTA CCAAGGAACC CACTAAGCAC CCTCGTGATC TTACAATGAT TGTCGCGGTT 
    TCTTCGCCAA ACCTCGGTAT CGGAAAGAAG AACTCCATGC CTTGGCATAT TAAACAAGAA 
    ATGGCTTACT TTGCCAATGT TACATCTTCT ACCGAAAGTA GCGGCCAACT GGAAGAAGGA 
    AAATCCAAGA TTATGAACGT TGTAATCATG GGTCGTAGTT GCTACGACAG TTTGCCCAAG 
    AAGAACCGTC CTTTGAAGGA TCGTATCAAC ATTGTTATTA CACGCAATTC TAACTACAAC 
    TTTGGTTTGA CCAAAAAAGA AAAGATGCCC GAGAATCTGT ATGCGGCTGA TTGTATTGAC 
    TCAGCATTGG ACCTCGTGGC TGAGAAGTAT GGAGCTGATA GCGACATCCA AGTTGGAAAA 
    GTGTTTATCA TTGGTGGCTC TTTCCTTTAT GGAAGTGCTC TTTACCATCC TTTGACCAAA 
    AACCTTCTTT TCACTCGTAT CCACAAAGAA TATCCTTGTG ATTCTTTCTT TCCCTTTGAA 
    CCAGCTGAAT CATCTGACTG GGTTAGAAAG GCTCATCCTG AATTGGAAAA GTTTGTTGGT 
    ATTCCGGTAG AGGAAGGTCG TCTGAAGGCA GCTTCCTCAA ACAAAGAAGA GGTTGAAATA 
    GAATTCGAGC TTTATGGAAA GAACGATGAT GTTAATGTAG CTTTAGAAAA GCTTAGTATT 
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_588353.1
    CDS163..1548
    Translation

    Target ORF information:

    RefSeq Version NM_001023344.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe dihydrofolate reductase Dfr1 (dfr1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001023344.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCTAAGC CTTTAAAAGT GCTGTGTCTT CATGGATGGA TTCAATCTGG TCCCGTATTT 
    TCAAAAAAAA TGGGATCCGT CCAGAAATAC CTGTCTAAGT ATGCCGAATT GCATTTTCCA 
    ACAGGTCCTG TGGTCGCTGA CGAAGAAGCA GACCCCAATG ATGAAGAAGA GAAGAAACGC 
    CTTGCTGCGC TTGGTGGTGA ACAAAACGGT GGTAAATTTG GGTGGTTTGA AGTGGAGGAT 
    TTTAAAAATA CATACGGAAG TTGGGATGAA AGTCTTGAAT GTATAAATCA ATACATGCAA 
    GAAAAAGGTC CCTTTGATGG ACTTATCGGA TTTTCTCAAG GTGCAGGAAT AGGTGCTATG 
    CTGGCTCAAA TGCTTCAGCC TGGTCAACCT CCCAACCCTT ATGTCCAACA TCCTCCTTTT 
    AAATTTGTTG TTTTTGTTGG CGGTTTCCGT GCTGAAAAGC CTGAATTCGA TCATTTTTAT 
    AACCCCAAGC TTACCACTCC TTCGTTACAT ATTGCTGGTA CTTCTGATAC CCTGGTCCCA 
    CTTGCTCGCT CCAAACAGTT GGTTGAACGT TGCGAAAATG CACACGTTTT GTTGCATCCG 
    GGACAACATA TTGTTCCTCA ACAAGCAGTG TATAAGACTG GCATCCGTGA TTTCATGTTT 
    TCCGCCCCTA CCAAGGAACC CACTAAGCAC CCTCGTGATC TTACAATGAT TGTCGCGGTT 
    TCTTCGCCAA ACCTCGGTAT CGGAAAGAAG AACTCCATGC CTTGGCATAT TAAACAAGAA 
    ATGGCTTACT TTGCCAATGT TACATCTTCT ACCGAAAGTA GCGGCCAACT GGAAGAAGGA 
    AAATCCAAGA TTATGAACGT TGTAATCATG GGTCGTAGTT GCTACGACAG TTTGCCCAAG 
    AAGAACCGTC CTTTGAAGGA TCGTATCAAC ATTGTTATTA CACGCAATTC TAACTACAAC 
    TTTGGTTTGA CCAAAAAAGA AAAGATGCCC GAGAATCTGT ATGCGGCTGA TTGTATTGAC 
    TCAGCATTGG ACCTCGTGGC TGAGAAGTAT GGAGCTGATA GCGACATCCA AGTTGGAAAA 
    GTGTTTATCA TTGGTGGCTC TTTCCTTTAT GGAAGTGCTC TTTACCATCC TTTGACCAAA 
    AACCTTCTTT TCACTCGTAT CCACAAAGAA TATCCTTGTG ATTCTTTCTT TCCCTTTGAA 
    CCAGCTGAAT CATCTGACTG GGTTAGAAAG GCTCATCCTG AATTGGAAAA GTTTGTTGGT 
    ATTCCGGTAG AGGAAGGTCG TCTGAAGGCA GCTTCCTCAA ACAAAGAAGA GGTTGAAATA 
    GAATTCGAGC TTTATGGAAA GAACGATGAT GTTAATGTAG CTTTAGAAAA GCTTAGTATT 
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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