MUS81 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene
Clones
gRNAs

Summary

Gene Symbol	MUS81
Entrez Gene ID	2539004
Full Name	Holliday junction resolvase subunit Mus81
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe 972h-

mRNA and Protein(s)

mRNA	Protein	Name
NM_001355879.1	NP_001343019.1	Holliday junction resolvase subunit Mus81
XM_001713109.1	XP_001713161.1	Holliday junction resolvase subunit Mus81

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008836	XP_319580.4
Pan troglodytes (chimpanzee)	MUS81	XP_522066.4
	MUS81	XP_001112824.1
Rattus norvegicus (Norway rat)	Mus81	NP_001020816.1
Eremothecium gossypii	AGOS_AFR141C	NP_985688.1
Magnaporthe oryzae (rice blast fungus)	MGG_04585	XP_003713609.1
Xenopus tropicalis (tropical clawed frog)	mus81	NP_001011004.1
Canis lupus familiaris (dog)	MUS81	XP_533230.2
Mus musculus (house mouse)	Mus81	NP_082153.3
Danio rerio (zebrafish)	mus81	NP_998683.1
Schizosaccharomyces pombe (fission yeast)	mus81	XP_001713161.1
Bos taurus (cattle)	MUS81	NP_001039756.1
Drosophila melanogaster (fruit fly)	mus81	NP_569873.1
Saccharomyces cerevisiae (baker's yeast)	MUS81	NP_010674.3
Kluyveromyces lactis	KLLA0E03015g	XP_454081.1
Neurospora crassa	NCU07457	XP_963908.1
Homo sapiens (human)	MUS81	NP_079404.3

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Removing Rhp51, the Rad51 recombinase homologue, suppresses the slowing defect of rqh1Delta mutants, but not that of mus81Delta mutant, defining an epistatic pathway in which mus81 is epistatic to rhp51 and rhp51 is epistatic to rqh1.
Title: Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.

Findings demonstrate that Mus81/Eme1 is the dedicated endonuclease that resolves sister chromatid recombination intermediates during the repair of broken replication forks.
Title: Mus81 is essential for sister chromatid recombination at broken replication forks.

An interplay between Mus81 activity at stalled forks and the Chk1-dependent DNA damage checkpoint during S-phase when replication forks have collapsed, was identified.
Title: Cleavage of stalled forks by fission yeast Mus81/Eme1 in absence of DNA replication checkpoint.

The purification of active forms of recombinant Schizosaccharomyces pombe Mus81-Eme1 and Saccharomyces cerevisiae Mus81-Mms4, which display robust Holliday junction cleavage in vitro, is reported.
Title: Mus81 cleavage of Holliday junctions: a failsafe for processing meiotic recombination intermediates?

Upon S-phase arrest by acute hydroxyurea treatment, Mus81 is not required for cell viability but is essential for recovery from replication fork collapse.
Title: Replication checkpoint kinase Cds1 regulates Mus81 to preserve genome integrity during replication stress.

The following MUS81 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MUS81 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSj00355	XM_001713109.1 Latest version!	Schizosaccharomyces pombe 972h- Holliday junction resolvase subunit Mus81 (mus81), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OSc99876	NM_001355879.1 Latest version!	Schizosaccharomyces pombe Holliday junction resolvase subunit Mus81 (mus81), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MUS81 ( XM_001713109.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> XP_001713161.1 Schizosaccharomyces pombe 972h- Holliday junction resolvase subunit Mus81 (mus81), mRNA.

Price & Availability↑

CloneID	OSj00355
Clone ID Related Accession (Same CDS sequence)	XM_001713109.1
Accession Version	XM_001713109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	Holliday junction resolvase subunit Mus81
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGGATTGTG GAAATCCGCT GTTTTTACAA TGGATACAGG AGTGGATGGA AGAGTCGACG 
CGTCGCTTCC CCAAGTCATA TCAAACCTGG AGAAAGGCCT ACGATTCAAT GAAAAGTTGC 
CCCATCACAT TTCATCGTCC TTCTCAGGCA TTGGCACTGA AGGGAATTGG CCCTACGATT 
TGTGCCAAGC TAGAAAAGAA ATGGAACGCT TACTGTTTGG AAAATAATAT TCCAATTAGC 
ACGCACAATG AACAGAATGA TTCTCATGTC AATGCTAATA AATCGTCTTC TGAGACTTCT 
TCTGAAAAGC CTCGAAGCGT TAAAAAACCG ACTACAAGAA AGAGAAAGGT CTATGTTCCG 
AGCTATCGTT CTGGTGCATA TTCAATTCTT TGCGCTCTTT ATATGCTTAA TAAACACGAA 
TTCGCTACAA AGCCTCAAAT TGTTACGATG GCCCAACCGT ATTGTGATTC GTCCTTTGGA 
TCTGCTACAG ACCGAAACAT GCGTTATACT GCATGGAGTG CTATGAAGAC TTTGATCACA 
AAAAATTTAG TTTACCAGAC AGGTCATCCC TCTAAGTACT GCTTGACCGA CGATGGTGAA 
GAAGTTTGCA TACGTTTGGC AAAAGTAGAC GATTCCTTTC AACGGAAACA TACTGTATCA 
AACTTTTCTG TCTCTAAATC CGATGATCAT GATTCCTCTT TATGCCAACC TCCAAATTTT 
GTTACATCAA TCAATAAAGC AGGAAGTTCT TCAGACCACG GAGGTGAACT ACATGTTACT 
TACTGTCCGG TAGATCATAA TGAAGTTTCT GATGGTGTTG AAACCGATAT AGATGTTGAT 
CAGGTGGATT CGTTGACCGG AATTCATGAT CACCATATCA TTAACAATGA GCAGTTGATT 
GACTTGACGG AACAAGAAAA AAAGCAACCA AATGAAAGCA ATTTGTCTAA CTTAAAAATC 
GAGACGGTAT TATTCTCAAA CTGTACGGTT TTTCTTCTGA TTGATACTCG AGAAATACGT 
TCTCCCTTGG ATCGCAATCT TATTATCGAT AAACTGACTA ATGACTTTGG TGTCAATTGT 
CAGGTACGAT CATTAGAATT GGGAGATGCT TTATGGGTTG CAAGGGATAT GGAAAGCGGT 
CAAGAAGTTG TGTTGGATTT TGTCGTTGAA AGGAAAAGAT ATGATGATTT AGTTGCTTCC 
ATTAAGGACG GAAGATTTCA CGAACAAAAG GCACGTCTAA AGAAGAGCGG AATACGTTCA 
GTCACCTACA TTTTGGAGGA ATCCTCTTAT GATGAAAGTT TCACGGAATC AATTCGTACA 
GCTGTTAGCA ATACCCAAGT CGATCAATTA TTTCATGTCC GTCATACGCG ATCACTTGAA 
CACAGCGTAA GTTTGCTTGC TGAAATGACA AAGCAAATTA ACCTGTTTTA TGAGAAACGT 
AAAACCCTCG CTGTCATTCC CGATTTGTCG ATAGAAGCCA AAACTTATGA GTCGTTACGA 
GAGCAACTTC TAAAAATTGA TCCCTCAACT CCTTACCACA TATCATACCA TGCATTTTCC 
TCTGTCCTCT CGAAGTCTTC TACTTTGACC GTGGGGGATA TTTTTATTCG AATGCTTATG 
ACTATAAAAG GTATAAGTGC TTCAAAAGCC ATCGAGATTC AGAAGAAATA TCCAACATTC 
ATGCATTTAT TTGAGGCGTA CGAAAAGTCA TCATCTAGCC AGGAAAGAAA CTTATTACTG 
AATAAGACTT GTCAAGGCTA TGGTTTTCAA ACAATAGGGC CAGCACTTTC CGCCAAGGTC 
GCAAGCGTTT TCTTTCCAGA ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713161.1
CDS	1..1827
Translation	MDCGNPLFLQWIQEWMEESTRRFPKSYQTWRKAYDSMKSCPITFHRPSQALALKGIGPTICAKLEKKWNAYCLENNIPISTHNEQNDSHVNANKSSSETSSEKPRSVKKPTTRKRKVYVPSYRSGAYSILCALYMLNKHEFATKPQIVTMAQPYCDSSFGSATDRNMRYTAWSAMKTLITKNLVYQTGHPSKYCLTDDGEEVCIRLAKVDDSFQRKHTVSNFSVSKSDDHDSSLCQPPNFVTSINKAGSSSDHGGELHVTYCPVDHNEVSDGVETDIDVDQVDSLTGIHDHHIINNEQLIDLTEQEKKQPNESNLSNLKIETVLFSNCTVFLLIDTREIRSPLDRNLIIDKLTNDFGVNCQVRSLELGDALWVARDMESGQEVVLDFVVERKRYDDLVASIKDGRFHEQKARLKKSGIRSVTYILEESSYDESFTESIRTAVSNTQVDQLFHVRHTRSLEHSVSLLAEMTKQINLFYEKRKTLAVIPDLSIEAKTYESLREQLLKIDPSTPYHISYHAFSSVLSKSSTLTVGDIFIRMLMTIKGISASKAIEIQKKYPTFMHLFEAYEKSSSSQERNLLLNKTCQGYGFQTIGPALSAKVASVFFPES

Target ORF information:

RefSeq Version	XM_001713109.1
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- Holliday junction resolvase subunit Mus81 (mus81), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713109.1

ORF Insert Sequence:

ATGGATTGTG GAAATCCGCT GTTTTTACAA TGGATACAGG AGTGGATGGA AGAGTCGACG 
CGTCGCTTCC CCAAGTCATA TCAAACCTGG AGAAAGGCCT ACGATTCAAT GAAAAGTTGC 
CCCATCACAT TTCATCGTCC TTCTCAGGCA TTGGCACTGA AGGGAATTGG CCCTACGATT 
TGTGCCAAGC TAGAAAAGAA ATGGAACGCT TACTGTTTGG AAAATAATAT TCCAATTAGC 
ACGCACAATG AACAGAATGA TTCTCATGTC AATGCTAATA AATCGTCTTC TGAGACTTCT 
TCTGAAAAGC CTCGAAGCGT TAAAAAACCG ACTACAAGAA AGAGAAAGGT CTATGTTCCG 
AGCTATCGTT CTGGTGCATA TTCAATTCTT TGCGCTCTTT ATATGCTTAA TAAACACGAA 
TTCGCTACAA AGCCTCAAAT TGTTACGATG GCCCAACCGT ATTGTGATTC GTCCTTTGGA 
TCTGCTACAG ACCGAAACAT GCGTTATACT GCATGGAGTG CTATGAAGAC TTTGATCACA 
AAAAATTTAG TTTACCAGAC AGGTCATCCC TCTAAGTACT GCTTGACCGA CGATGGTGAA 
GAAGTTTGCA TACGTTTGGC AAAAGTAGAC GATTCCTTTC AACGGAAACA TACTGTATCA 
AACTTTTCTG TCTCTAAATC CGATGATCAT GATTCCTCTT TATGCCAACC TCCAAATTTT 
GTTACATCAA TCAATAAAGC AGGAAGTTCT TCAGACCACG GAGGTGAACT ACATGTTACT 
TACTGTCCGG TAGATCATAA TGAAGTTTCT GATGGTGTTG AAACCGATAT AGATGTTGAT 
CAGGTGGATT CGTTGACCGG AATTCATGAT CACCATATCA TTAACAATGA GCAGTTGATT 
GACTTGACGG AACAAGAAAA AAAGCAACCA AATGAAAGCA ATTTGTCTAA CTTAAAAATC 
GAGACGGTAT TATTCTCAAA CTGTACGGTT TTTCTTCTGA TTGATACTCG AGAAATACGT 
TCTCCCTTGG ATCGCAATCT TATTATCGAT AAACTGACTA ATGACTTTGG TGTCAATTGT 
CAGGTACGAT CATTAGAATT GGGAGATGCT TTATGGGTTG CAAGGGATAT GGAAAGCGGT 
CAAGAAGTTG TGTTGGATTT TGTCGTTGAA AGGAAAAGAT ATGATGATTT AGTTGCTTCC 
ATTAAGGACG GAAGATTTCA CGAACAAAAG GCACGTCTAA AGAAGAGCGG AATACGTTCA 
GTCACCTACA TTTTGGAGGA ATCCTCTTAT GATGAAAGTT TCACGGAATC AATTCGTACA 
GCTGTTAGCA ATACCCAAGT CGATCAATTA TTTCATGTCC GTCATACGCG ATCACTTGAA 
CACAGCGTAA GTTTGCTTGC TGAAATGACA AAGCAAATTA ACCTGTTTTA TGAGAAACGT 
AAAACCCTCG CTGTCATTCC CGATTTGTCG ATAGAAGCCA AAACTTATGA GTCGTTACGA 
GAGCAACTTC TAAAAATTGA TCCCTCAACT CCTTACCACA TATCATACCA TGCATTTTCC 
TCTGTCCTCT CGAAGTCTTC TACTTTGACC GTGGGGGATA TTTTTATTCG AATGCTTATG 
ACTATAAAAG GTATAAGTGC TTCAAAAGCC ATCGAGATTC AGAAGAAATA TCCAACATTC 
ATGCATTTAT TTGAGGCGTA CGAAAAGTCA TCATCTAGCC AGGAAAGAAA CTTATTACTG 
AATAAGACTT GTCAAGGCTA TGGTTTTCAA ACAATAGGGC CAGCACTTTC CGCCAAGGTC 
GCAAGCGTTT TCTTTCCAGA ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MUS81 ( NM_001355879.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_001343019.1 Schizosaccharomyces pombe Holliday junction resolvase subunit Mus81 (mus81), partial mRNA.

Price & Availability↑

CloneID	OSc99876
Clone ID Related Accession (Same CDS sequence)	NM_001355879.1
Accession Version	NM_001355879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Holliday junction resolvase subunit Mus81
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). COMPLETENESS: incomplete on both ends.

ATGGATTGTG GAAATCCGCT GTTTTTACAA TGGATACAGG AGTGGATGGA AGAGTCGACG 
CGTCGCTTCC CCAAGTCATA TCAAACCTGG AGAAAGGCCT ACGATTCAAT GAAAAGTTGC 
CCCATCACAT TTCATCGTCC TTCTCAGGCA TTGGCACTGA AGGGAATTGG CCCTACGATT 
TGTGCCAAGC TAGAAAAGAA ATGGAACGCT TACTGTTTGG AAAATAATAT TCCAATTAGC 
ACGCACAATG AACAGAATGA TTCTCATGTC AATGCTAATA AATCGTCTTC TGAGACTTCT 
TCTGAAAAGC CTCGAAGCGT TAAAAAACCG ACTACAAGAA AGAGAAAGGT CTATGTTCCG 
AGCTATCGTT CTGGTGCATA TTCAATTCTT TGCGCTCTTT ATATGCTTAA TAAACACGAA 
TTCGCTACAA AGCCTCAAAT TGTTACGATG GCCCAACCGT ATTGTGATTC GTCCTTTGGA 
TCTGCTACAG ACCGAAACAT GCGTTATACT GCATGGAGTG CTATGAAGAC TTTGATCACA 
AAAAATTTAG TTTACCAGAC AGGTCATCCC TCTAAGTACT GCTTGACCGA CGATGGTGAA 
GAAGTTTGCA TACGTTTGGC AAAAGTAGAC GATTCCTTTC AACGGAAACA TACTGTATCA 
AACTTTTCTG TCTCTAAATC CGATGATCAT GATTCCTCTT TATGCCAACC TCCAAATTTT 
GTTACATCAA TCAATAAAGC AGGAAGTTCT TCAGACCACG GAGGTGAACT ACATGTTACT 
TACTGTCCGG TAGATCATAA TGAAGTTTCT GATGGTGTTG AAACCGATAT AGATGTTGAT 
CAGGTGGATT CGTTGACCGG AATTCATGAT CACCATATCA TTAACAATGA GCAGTTGATT 
GACTTGACGG AACAAGAAAA AAAGCAACCA AATGAAAGCA ATTTGTCTAA CTTAAAAATC 
GAGACGGTAT TATTCTCAAA CTGTACGGTT TTTCTTCTGA TTGATACTCG AGAAATACGT 
TCTCCCTTGG ATCGCAATCT TATTATCGAT AAACTGACTA ATGACTTTGG TGTCAATTGT 
CAGGTACGAT CATTAGAATT GGGAGATGCT TTATGGGTTG CAAGGGATAT GGAAAGCGGT 
CAAGAAGTTG TGTTGGATTT TGTCGTTGAA AGGAAAAGAT ATGATGATTT AGTTGCTTCC 
ATTAAGGACG GAAGATTTCA CGAACAAAAG GCACGTCTAA AGAAGAGCGG AATACGTTCA 
GTCACCTACA TTTTGGAGGA ATCCTCTTAT GATGAAAGTT TCACGGAATC AATTCGTACA 
GCTGTTAGCA ATACCCAAGT CGATCAATTA TTTCATGTCC GTCATACGCG ATCACTTGAA 
CACAGCGTAA GTTTGCTTGC TGAAATGACA AAGCAAATTA ACCTGTTTTA TGAGAAACGT 
AAAACCCTCG CTGTCATTCC CGATTTGTCG ATAGAAGCCA AAACTTATGA GTCGTTACGA 
GAGCAACTTC TAAAAATTGA TCCCTCAACT CCTTACCACA TATCATACCA TGCATTTTCC 
TCTGTCCTCT CGAAGTCTTC TACTTTGACC GTGGGGGATA TTTTTATTCG AATGCTTATG 
ACTATAAAAG GTATAAGTGC TTCAAAAGCC ATCGAGATTC AGAAGAAATA TCCAACATTC 
ATGCATTTAT TTGAGGCGTA CGAAAAGTCA TCATCTAGCC AGGAAAGAAA CTTATTACTG 
AATAAGACTT GTCAAGGCTA TGGTTTTCAA ACAATAGGGC CAGCACTTTC CGCCAAGGTC 
GCAAGCGTTT TCTTTCCAGA ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343019.1
CDS	1..1827
Translation	MDCGNPLFLQWIQEWMEESTRRFPKSYQTWRKAYDSMKSCPITFHRPSQALALKGIGPTICAKLEKKWNAYCLENNIPISTHNEQNDSHVNANKSSSETSSEKPRSVKKPTTRKRKVYVPSYRSGAYSILCALYMLNKHEFATKPQIVTMAQPYCDSSFGSATDRNMRYTAWSAMKTLITKNLVYQTGHPSKYCLTDDGEEVCIRLAKVDDSFQRKHTVSNFSVSKSDDHDSSLCQPPNFVTSINKAGSSSDHGGELHVTYCPVDHNEVSDGVETDIDVDQVDSLTGIHDHHIINNEQLIDLTEQEKKQPNESNLSNLKIETVLFSNCTVFLLIDTREIRSPLDRNLIIDKLTNDFGVNCQVRSLELGDALWVARDMESGQEVVLDFVVERKRYDDLVASIKDGRFHEQKARLKKSGIRSVTYILEESSYDESFTESIRTAVSNTQVDQLFHVRHTRSLEHSVSLLAEMTKQINLFYEKRKTLAVIPDLSIEAKTYESLREQLLKIDPSTPYHISYHAFSSVLSKSSTLTVGDIFIRMLMTIKGISASKAIEIQKKYPTFMHLFEAYEKSSSSQERNLLLNKTCQGYGFQTIGPALSAKVASVFFPES

Target ORF information:

RefSeq Version	NM_001355879.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Holliday junction resolvase subunit Mus81 (mus81), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355879.1

ORF Insert Sequence:

ATGGATTGTG GAAATCCGCT GTTTTTACAA TGGATACAGG AGTGGATGGA AGAGTCGACG 
CGTCGCTTCC CCAAGTCATA TCAAACCTGG AGAAAGGCCT ACGATTCAAT GAAAAGTTGC 
CCCATCACAT TTCATCGTCC TTCTCAGGCA TTGGCACTGA AGGGAATTGG CCCTACGATT 
TGTGCCAAGC TAGAAAAGAA ATGGAACGCT TACTGTTTGG AAAATAATAT TCCAATTAGC 
ACGCACAATG AACAGAATGA TTCTCATGTC AATGCTAATA AATCGTCTTC TGAGACTTCT 
TCTGAAAAGC CTCGAAGCGT TAAAAAACCG ACTACAAGAA AGAGAAAGGT CTATGTTCCG 
AGCTATCGTT CTGGTGCATA TTCAATTCTT TGCGCTCTTT ATATGCTTAA TAAACACGAA 
TTCGCTACAA AGCCTCAAAT TGTTACGATG GCCCAACCGT ATTGTGATTC GTCCTTTGGA 
TCTGCTACAG ACCGAAACAT GCGTTATACT GCATGGAGTG CTATGAAGAC TTTGATCACA 
AAAAATTTAG TTTACCAGAC AGGTCATCCC TCTAAGTACT GCTTGACCGA CGATGGTGAA 
GAAGTTTGCA TACGTTTGGC AAAAGTAGAC GATTCCTTTC AACGGAAACA TACTGTATCA 
AACTTTTCTG TCTCTAAATC CGATGATCAT GATTCCTCTT TATGCCAACC TCCAAATTTT 
GTTACATCAA TCAATAAAGC AGGAAGTTCT TCAGACCACG GAGGTGAACT ACATGTTACT 
TACTGTCCGG TAGATCATAA TGAAGTTTCT GATGGTGTTG AAACCGATAT AGATGTTGAT 
CAGGTGGATT CGTTGACCGG AATTCATGAT CACCATATCA TTAACAATGA GCAGTTGATT 
GACTTGACGG AACAAGAAAA AAAGCAACCA AATGAAAGCA ATTTGTCTAA CTTAAAAATC 
GAGACGGTAT TATTCTCAAA CTGTACGGTT TTTCTTCTGA TTGATACTCG AGAAATACGT 
TCTCCCTTGG ATCGCAATCT TATTATCGAT AAACTGACTA ATGACTTTGG TGTCAATTGT 
CAGGTACGAT CATTAGAATT GGGAGATGCT TTATGGGTTG CAAGGGATAT GGAAAGCGGT 
CAAGAAGTTG TGTTGGATTT TGTCGTTGAA AGGAAAAGAT ATGATGATTT AGTTGCTTCC 
ATTAAGGACG GAAGATTTCA CGAACAAAAG GCACGTCTAA AGAAGAGCGG AATACGTTCA 
GTCACCTACA TTTTGGAGGA ATCCTCTTAT GATGAAAGTT TCACGGAATC AATTCGTACA 
GCTGTTAGCA ATACCCAAGT CGATCAATTA TTTCATGTCC GTCATACGCG ATCACTTGAA 
CACAGCGTAA GTTTGCTTGC TGAAATGACA AAGCAAATTA ACCTGTTTTA TGAGAAACGT 
AAAACCCTCG CTGTCATTCC CGATTTGTCG ATAGAAGCCA AAACTTATGA GTCGTTACGA 
GAGCAACTTC TAAAAATTGA TCCCTCAACT CCTTACCACA TATCATACCA TGCATTTTCC 
TCTGTCCTCT CGAAGTCTTC TACTTTGACC GTGGGGGATA TTTTTATTCG AATGCTTATG 
ACTATAAAAG GTATAAGTGC TTCAAAAGCC ATCGAGATTC AGAAGAAATA TCCAACATTC 
ATGCATTTAT TTGAGGCGTA CGAAAAGTCA TCATCTAGCC AGGAAAGAAA CTTATTACTG 
AATAAGACTT GTCAAGGCTA TGGTTTTCAA ACAATAGGGC CAGCACTTTC CGCCAAGGTC 
GCAAGCGTTT TCTTTCCAGA ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
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MUS81 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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