ers1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ers1
Entrez Gene ID	2538690
Full Name	RNA-silencing factor Ers1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022954.2	NP_587963.1	RNA-silencing factor Ers1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ers1 may promote RNAi by tethering the corresponding enzyme complexes to HP1-coated chromatin, thereby placing them in proximity to the nascent noncoding RNA substrate
Title: Ers1 links HP1 to RNAi.

Data show that although Ers1 failed to bind Clr4, a direct interaction with Swi6 was detected, and centromeric localization of Swi6 was enhanced by Clr4 overexpression.
Title: Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.

The following ers1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ers1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99681	NM_001022954.2 Latest version!	Schizosaccharomyces pombe RNA-silencing factor Ers1 (ers1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ers1 ( NM_001022954.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_587963.1 Schizosaccharomyces pombe RNA-silencing factor Ers1 (ers1), mRNA.

Price & Availability↑

CloneID	OSc99681
Clone ID Related Accession (Same CDS sequence)	NM_001022954.2
Accession Version	NM_001022954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	RNA-silencing factor Ers1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022954.1.

ATGGGTAAAG TATCTGCAAA CAATCAATCT GGATTTTTTA AATTTTATAT CGATACAGCG 
TTGCTTTTAA ATGCTGAATT TATATCCTCC TTCACAAATT GTATAAATTC TGAATATAAT 
GGAAAAGTAA AAATTTCTTA CTCTTCTTCA TCTCAGCAAT TCAAAGTCAT AGTGTACGAG 
GAACACAAAA GAGAAGCGTT TAGTCTTTTT GAGGAAATAG TTCAAAAGTT GAAGCGCGAA 
TCAACTGTTT ACAAGAAACC ACGCCTAGAT ATTCATCCAT TCGGGCAATT GCATTATGAT 
TTTACTACTT TATTGAATTT TCAAAGGTAC TTAGACTTAG GATACGGTAT TGAAGATGTG 
AAAATAAAAG AAAATGAGGA CATTGTTAAG TCTAAAGTGG GCAAGAAACC GACATCCTCC 
GATTTTTATA TTCCTTGCTT GATAAAACCT AAAATCATTA CTCCTTTTGA ATGGGAAGGA 
AATTTTAAAC AGGTCATTTA TTCGCCTACT GTTTACAACG GGAGTTTCAA GCGATTATTT 
TACCTTCCCG ACAATAATGA TTTGCTCAAG GAGTTTGCGC ATGCTACCAA CACAAAATAC 
AAGGCTTCTT TAAAAGATAA TAAAATAGTT GTATTAGCCA ATAACGATCA GGACATTAAT 
GCTATGTTGG AGAAAATGAA GCATATAGAG AATTTAGTGT CCCAGAAAGT CTATGAATAT 
CCTGAAGAAA CTCTTGTTTA TGCAGGAAAG ATTGCACCCT GCCAACTGAA ATTTCTAAAA 
TTAAAATTTG AAAACGAGAT TTTATACACA GCTATGCATA CGACATCTAT TTTCAAAAGC 
GTTTTCAATA TCCTTGGATC TTTTTATACT ATGAGATTAT TGAGAAATAA ACATGATGGT 
GATTCTTATG AGTTGGTACC AGTTTTTGCT AAAAATCCTT CCAAAACCCC TCCCTGTAAT 
GATAATTTTA AAATATTTTC TGATGATATA AAGCTGATAG GATGTAAAAT ATTTGCAGGT 
GTTTCTTTAA ACTTCTCTTC TCCGAAACCA GCGCACAGAT TTTACGAGTT AAATAAAACT 
TCATCCAATT TGTCGATCCC AGTCTTACAA AAGCCGTCAA ATTTTCATTC TTCGTCAACC 
GAATTATCTG ATAATTCAAT TCATCAAGGA AGGAGAGCAG TTGATCCTGT TGTTAACCAG 
AATAATCCGT CAAATTTTGA AGAAATGATT ATGAACAAGT TGAATAAATT ACCCACGATT 
GATAAGCAAA TTTTAGGAAC TTCATCTTTG ACCCATTTCC AAGATAAAAC AACTGCAATT 
GAGCATTCAA TTAATAAGTC TAATAGCAAG CAGCCTCCTC GGTTCAAATT TCAGCTTCCT 
CCTCGACCTA CGAGCAATAC TTTACCATTG GAACCTGAAG AAGAGCTGGT TACTAGATAT 
TCTGTTTCTT CGGATGGTAA TACTGTTGAC GAAGCAATAA CTAAACAGTC TCAGACTTTT 
CAATTGGTCA ATTCAAATGA ATTTAATGAA GTTAACGCTA ATGATGTTCA TAAATCTTTG 
CGACAAAATT GCGCAAAATT GGATTTCGAC GATTCAAAGT CTAAAAACCT ATTAAGTGTA 
GAATGTTTAG AGCTTGACAA AGGATCAGAT TGTTCCACTC CAAAATCTGG CAGCTTAACT 
CCTTCAATTG ACATGAAGTT TTTACGTTTA CAAGATGAGA AAATGGACGA CCTTGGTGAC 
AACTATTATA CAATTCTAAT GTCTTCCAAT CCGGTATCAA GCTACGGTGT GGGAAGCTTA 
TACTTATTTC AACCAAAAAT AGTATGCTCT GAAAAATACA TTAATCACGA AGAGATTGAC 
AACATGAATT TGAAATCATT GCACAGATGG TTATCTAGAA GCTTGCATGT ACTTCAATCC 
TTTTCAGGTG AAATTGAATT GAATTTAGAG TTTGGCGTCA TACTATATCC AAATATCTCA 
TCAGATGTGT CGGCTTGTTC ACATGGTTTT ATGAATATAT ATAAGGATTT GAATCTTCCC 
CGATCATATT TTGCAGACTG CATTACCAAA TCAGTTTCGA ATATCGATTC ATTATTGAAT 
ACTCCTGTGA AAATTTTGTT TGGACGTACT GAGTATACTT ATCCTTTAAT AGAGCATGAA 
GTATTAGATT CAAAGAATTA CTTTGTTTTC AAGGGAAGTT TATTATTTAC AGATGATAAG 
AGAAACAAGA CAGAGGATTC CACTGTATTT TTTTCGATGA TTTGTTCTTC AAAGTTAGAT 
CAGTTTGCGT TTTATAAAGA TTCTAAGCAA TCTTCTACTT GCACTATCAA TTTTCCTTTA 
GCCTTATGGG ATTCCCGTCT ACGTATCGAA ACGAAAGTCC CTTTAAATGA TGCGATACTT 
ACAGAGTTCT CCAAAAGTAT TAGATTTAGA AACGTAAATA AGGATTTACT GTTAGTCTTT 
GGAAATATGG AAGACAGAGT AATAATTCAT TCGGTCACAA GAATTAATGA AAACTCTGTA 
CCTTTTAACA AAAATTTACT CCCTCACTCT CTAGAATTTA TATTAAAATG TTCAAAAGTT 
AAGAGCTATG ACATTAGCCC GTCATCTATT AATTCCAAAG AAGCCTTCGT TTGTCTCGAC 
AGATCCAAGG AAAGCAAACC TATAGAATCC TGTTTCAGTC TAAGTATACA AAGTGTGTAC 
ATGCAGTCTC AGTTCAAATA TAATAACAGT ATACGCGCAG GAGAAACGGC TCCGTGGAAA 
CTTGCAAATA AACAATTCAT TGGTGGGGCT ATTACTGAGA ATGTTTCAGA CTTGTATACT 
GCAGCAATTA TTATGGTGAA TCAATTAGGG GGGATACACC CTACTATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_587963.1
CDS	49..2919
Translation	MGKVSANNQSGFFKFYIDTALLLNAEFISSFTNCINSEYNGKVKISYSSSSQQFKVIVYEEHKREAFSLFEEIVQKLKRESTVYKKPRLDIHPFGQLHYDFTTLLNFQRYLDLGYGIEDVKIKENEDIVKSKVGKKPTSSDFYIPCLIKPKIITPFEWEGNFKQVIYSPTVYNGSFKRLFYLPDNNDLLKEFAHATNTKYKASLKDNKIVVLANNDQDINAMLEKMKHIENLVSQKVYEYPEETLVYAGKIAPCQLKFLKLKFENEILYTAMHTTSIFKSVFNILGSFYTMRLLRNKHDGDSYELVPVFAKNPSKTPPCNDNFKIFSDDIKLIGCKIFAGVSLNFSSPKPAHRFYELNKTSSNLSIPVLQKPSNFHSSSTELSDNSIHQGRRAVDPVVNQNNPSNFEEMIMNKLNKLPTIDKQILGTSSLTHFQDKTTAIEHSINKSNSKQPPRFKFQLPPRPTSNTLPLEPEEELVTRYSVSSDGNTVDEAITKQSQTFQLVNSNEFNEVNANDVHKSLRQNCAKLDFDDSKSKNLLSVECLELDKGSDCSTPKSGSLTPSIDMKFLRLQDEKMDDLGDNYYTILMSSNPVSSYGVGSLYLFQPKIVCSEKYINHEEIDNMNLKSLHRWLSRSLHVLQSFSGEIELNLEFGVILYPNISSDVSACSHGFMNIYKDLNLPRSYFADCITKSVSNIDSLLNTPVKILFGRTEYTYPLIEHEVLDSKNYFVFKGSLLFTDDKRNKTEDSTVFFSMICSSKLDQFAFYKDSKQSSTCTINFPLALWDSRLRIETKVPLNDAILTEFSKSIRFRNVNKDLLLVFGNMEDRVIIHSVTRINENSVPFNKNLLPHSLEFILKCSKVKSYDISPSSINSKEAFVCLDRSKESKPIESCFSLSIQSVYMQSQFKYNNSIRAGETAPWKLANKQFIGGAITENVSDLYTAAIIMVNQLGGIHPTI

Target ORF information:

RefSeq Version	NM_001022954.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe RNA-silencing factor Ers1 (ers1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022954.2

ORF Insert Sequence:

ATGGGTAAAG TATCTGCAAA CAATCAATCT GGATTTTTTA AATTTTATAT CGATACAGCG 
TTGCTTTTAA ATGCTGAATT TATATCCTCC TTCACAAATT GTATAAATTC TGAATATAAT 
GGAAAAGTAA AAATTTCTTA CTCTTCTTCA TCTCAGCAAT TCAAAGTCAT AGTGTACGAG 
GAACACAAAA GAGAAGCGTT TAGTCTTTTT GAGGAAATAG TTCAAAAGTT GAAGCGCGAA 
TCAACTGTTT ACAAGAAACC ACGCCTAGAT ATTCATCCAT TCGGGCAATT GCATTATGAT 
TTTACTACTT TATTGAATTT TCAAAGGTAC TTAGACTTAG GATACGGTAT TGAAGATGTG 
AAAATAAAAG AAAATGAGGA CATTGTTAAG TCTAAAGTGG GCAAGAAACC GACATCCTCC 
GATTTTTATA TTCCTTGCTT GATAAAACCT AAAATCATTA CTCCTTTTGA ATGGGAAGGA 
AATTTTAAAC AGGTCATTTA TTCGCCTACT GTTTACAACG GGAGTTTCAA GCGATTATTT 
TACCTTCCCG ACAATAATGA TTTGCTCAAG GAGTTTGCGC ATGCTACCAA CACAAAATAC 
AAGGCTTCTT TAAAAGATAA TAAAATAGTT GTATTAGCCA ATAACGATCA GGACATTAAT 
GCTATGTTGG AGAAAATGAA GCATATAGAG AATTTAGTGT CCCAGAAAGT CTATGAATAT 
CCTGAAGAAA CTCTTGTTTA TGCAGGAAAG ATTGCACCCT GCCAACTGAA ATTTCTAAAA 
TTAAAATTTG AAAACGAGAT TTTATACACA GCTATGCATA CGACATCTAT TTTCAAAAGC 
GTTTTCAATA TCCTTGGATC TTTTTATACT ATGAGATTAT TGAGAAATAA ACATGATGGT 
GATTCTTATG AGTTGGTACC AGTTTTTGCT AAAAATCCTT CCAAAACCCC TCCCTGTAAT 
GATAATTTTA AAATATTTTC TGATGATATA AAGCTGATAG GATGTAAAAT ATTTGCAGGT 
GTTTCTTTAA ACTTCTCTTC TCCGAAACCA GCGCACAGAT TTTACGAGTT AAATAAAACT 
TCATCCAATT TGTCGATCCC AGTCTTACAA AAGCCGTCAA ATTTTCATTC TTCGTCAACC 
GAATTATCTG ATAATTCAAT TCATCAAGGA AGGAGAGCAG TTGATCCTGT TGTTAACCAG 
AATAATCCGT CAAATTTTGA AGAAATGATT ATGAACAAGT TGAATAAATT ACCCACGATT 
GATAAGCAAA TTTTAGGAAC TTCATCTTTG ACCCATTTCC AAGATAAAAC AACTGCAATT 
GAGCATTCAA TTAATAAGTC TAATAGCAAG CAGCCTCCTC GGTTCAAATT TCAGCTTCCT 
CCTCGACCTA CGAGCAATAC TTTACCATTG GAACCTGAAG AAGAGCTGGT TACTAGATAT 
TCTGTTTCTT CGGATGGTAA TACTGTTGAC GAAGCAATAA CTAAACAGTC TCAGACTTTT 
CAATTGGTCA ATTCAAATGA ATTTAATGAA GTTAACGCTA ATGATGTTCA TAAATCTTTG 
CGACAAAATT GCGCAAAATT GGATTTCGAC GATTCAAAGT CTAAAAACCT ATTAAGTGTA 
GAATGTTTAG AGCTTGACAA AGGATCAGAT TGTTCCACTC CAAAATCTGG CAGCTTAACT 
CCTTCAATTG ACATGAAGTT TTTACGTTTA CAAGATGAGA AAATGGACGA CCTTGGTGAC 
AACTATTATA CAATTCTAAT GTCTTCCAAT CCGGTATCAA GCTACGGTGT GGGAAGCTTA 
TACTTATTTC AACCAAAAAT AGTATGCTCT GAAAAATACA TTAATCACGA AGAGATTGAC 
AACATGAATT TGAAATCATT GCACAGATGG TTATCTAGAA GCTTGCATGT ACTTCAATCC 
TTTTCAGGTG AAATTGAATT GAATTTAGAG TTTGGCGTCA TACTATATCC AAATATCTCA 
TCAGATGTGT CGGCTTGTTC ACATGGTTTT ATGAATATAT ATAAGGATTT GAATCTTCCC 
CGATCATATT TTGCAGACTG CATTACCAAA TCAGTTTCGA ATATCGATTC ATTATTGAAT 
ACTCCTGTGA AAATTTTGTT TGGACGTACT GAGTATACTT ATCCTTTAAT AGAGCATGAA 
GTATTAGATT CAAAGAATTA CTTTGTTTTC AAGGGAAGTT TATTATTTAC AGATGATAAG 
AGAAACAAGA CAGAGGATTC CACTGTATTT TTTTCGATGA TTTGTTCTTC AAAGTTAGAT 
CAGTTTGCGT TTTATAAAGA TTCTAAGCAA TCTTCTACTT GCACTATCAA TTTTCCTTTA 
GCCTTATGGG ATTCCCGTCT ACGTATCGAA ACGAAAGTCC CTTTAAATGA TGCGATACTT 
ACAGAGTTCT CCAAAAGTAT TAGATTTAGA AACGTAAATA AGGATTTACT GTTAGTCTTT 
GGAAATATGG AAGACAGAGT AATAATTCAT TCGGTCACAA GAATTAATGA AAACTCTGTA 
CCTTTTAACA AAAATTTACT CCCTCACTCT CTAGAATTTA TATTAAAATG TTCAAAAGTT 
AAGAGCTATG ACATTAGCCC GTCATCTATT AATTCCAAAG AAGCCTTCGT TTGTCTCGAC 
AGATCCAAGG AAAGCAAACC TATAGAATCC TGTTTCAGTC TAAGTATACA AAGTGTGTAC 
ATGCAGTCTC AGTTCAAATA TAATAACAGT ATACGCGCAG GAGAAACGGC TCCGTGGAAA 
CTTGCAAATA AACAATTCAT TGGTGGGGCT ATTACTGAGA ATGTTTCAGA CTTGTATACT 
GCAGCAATTA TTATGGTGAA TCAATTAGGG GGGATACACC CTACTATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ers1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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