SWI4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SWI4
Entrez Gene ID	856847
Full Name	SBF complex DNA-binding subunit SWI4
Synonyms	ART1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179001.1	NP_011036.1	SBF complex DNA-binding subunit SWI4

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SWI4	NP_011036.1
Eremothecium gossypii	AGOS_AGL297C	NP_986370.1
Kluyveromyces lactis	KLLA0E20791g	XP_454890.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors show that a functionally similar ART sorting signal is also required for the endocytosis of a second Art1-dependent cargo, Can1, suggesting a common mechanism for recognition of Art1 substrates.
Title: Identification of the endocytic sorting signal recognized by the Art1-Rsp5 ubiquitin ligase complex.

expression of mutated Swi4(S159A) protein in the GG104-Swi4Delta strain did not restore the transient G1 arrest induced by PKA activation, suggesting that Swi4 phosphorylation by PKA regulates CLN1 gene expression and G1/S phase transition.
Title: The transcription factor Swi4 is target for PKA regulation of cell size at the G1 to S transition in Saccharomyces cerevisiae.

Swi4/Swi6 (SBF) factor binding at HO follows a temporal cascade, with SBF bound at upstream regulatory sequence (URS2-L) serving to relay a signal from URS1 to the SBF sites in URS2-R that ultimately activate gene expression.
Title: Spatiotemporal cascade of transcription factor binding required for promoter activation.

We found that the transcriptional cofactor Swi6p and activator Swi4p, as well as the G(2)/M-specific cyclin Clb2p, are required in a microtubule-destabilizing environment
Title: Cell cycle regulators interact with pathways that modulate microtubule stability in Saccharomyces cerevisiae.

Study shows that Npr1 inhibits the activity of Art1, an arrestin-like adaptor protein that promotes endocytosis by targeting the Rsp5 ubiquitin ligase to specific plasma membrane cargoes.
Title: TORC1 regulates endocytosis via Npr1-mediated phosphoinhibition of a ubiquitin ligase adaptor.

The following SWI4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SWI4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05606	NM_001179001.1 Latest version!	Saccharomyces cerevisiae S288C SBF complex DNA-binding subunit SWI4 (SWI4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SWI4 ( NM_001179001.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011036.1 Saccharomyces cerevisiae S288C SBF complex DNA-binding subunit SWI4 (SWI4), partial mRNA.

Price & Availability↑

CloneID	OSi05606
Clone ID Related Accession (Same CDS sequence)	NM_001179001.1
Accession Version	NM_001179001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	SBF complex DNA-binding subunit SWI4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCATTTG ATGTTTTGAT ATCAAACCAA AAAGATAATA CCAATCACCA GAACATCACG 
CCAATATCAA AATCAGTTCT TTTGGCGCCT CATTCAAACC ATCCAGTGAT TGAAATAGCT 
ACGTATTCAG AAACCGATGT ATACGAATGC TATATACGTG GGTTTGAAAC TAAAATAGTG 
ATGAGAAGAA CTAAAGATGA TTGGATTAAT ATTACCCAGG TTTTCAAAAT AGCTCAATTT 
TCGAAGACGA AAAGAACTAA AATACTGGAA AAGGAATCAA ATGATATGCA ACATGAAAAA 
GTTCAAGGTG GGTATGGTAG GTTTCAAGGT ACTTGGATCC CACTGGATTC AGCGAAGTTT 
TTAGTCAATA AGTACGAAAT AATTGACCCT GTGGTCAACT CCATCTTAAC TTTTCAATTC 
GATCCAAATA ACCCTCCACC AAAGAGAAGC AAAAATAGTA TATTGAGGAA GACTTCGCCG 
GGAACGAAGA TAACTTCTCC ATCGAGCTAC AATAAAACAC CAAGAAAAAA GAACAGTAGT 
TCATCAACAT CGGCTACAAC TACAGCTGCG AATAAGAAAG GCAAGAAAAA TGCATCGATC 
AACCAGCCGA ATCCAAGTCC TTTACAAAAC CTTGTTTTCC AAACTCCTCA ACAGTTCCAA 
GTAAATTCAT CAATGAATAT AATGAACAAT AATGATAACC ACACCACGAT GAATTTCAAC 
AATGACACAA GACACAATTT AATAAATAAT ATTAGTAACA ACAGCAATCA ATCTACTATT 
ATTCAACAAC AAAAATCCAT TCATGAAAAC TCTTTCAATA ATAATTATTC GGCAACTCAG 
AAACCTCTTC AATTTTTTCC AATTCCGACA AATTTACAAA ACAAGAACGT TGCTCTGAAT 
AACCCTAATA ATAATGACAG TAATAGTTAC AGCCATAACA TCGACAATGT CATTAACAGC 
AGTAATAACA ATAATAATGG CAATAATAAT AACTTGATCA TAGTTCCAGA CGGACCTATG 
CAAAGCCAAC AACAACAACA ACATCATCAT GAATATTTGA CGAATAACTT CAATCATTCC 
ATGATGGACT CCATTACAAA TGGCAATAGT AAAAAAAGGA GGAAAAAGCT GAATCAATCT 
AATGAACAAC AATTTTACAA CCAGCAAGAA AAAATACAAA GGCATTTCAA ATTAATGAAG 
CAACCCTTAT TATGGCAATC CTTTCAAAAT CCAAATGATC ACCATAATGA GTACTGTGAC 
AGTAACGGCA GCAACAATAA CAACAACACT GTAGCATCCA ATGGCTCCTC CATTGAAGTA 
TTTTCATCAA ATGAAAATGA TAACAGTATG AATATGTCTT CACGATCTAT GACACCGTTC 
AGCGCAGGAA ATACTTCATC TCAAAACAAG TTGGAAAACA AAATGACAGA TCAAGAATAC 
AAGCAGACGA TATTAACAAT ACTATCATCG GAAAGGTCTT CTGATGTAGA TCAAGCGTTG 
TTGGCTACTT TATATCCGGC GCCCAAAAAT TTTAACATAA ATTTTGAAAT TGATGATCAA 
GGTCACACTC CACTCCATTG GGCTACAGCT ATGGCGAATA TTCCTTTGAT AAAAATGCTA 
ATTACCTTAA ATGCTAATGC ATTACAGTGT AATAAGCTCG GTTTTAATTG CATCACAAAA 
TCAATTTTCT ACAACAATTG CTACAAGGAG AATGCTTTCG ATGAAATAAT ATCCATCTTA 
AAAATATGTC TTATAACTCC CGACGTAAAT GGAAGATTGC CATTTCATTA TTTAATTGAA 
TTGAGCGTTA ATAAGTCCAA AAATCCAATG ATAATAAAAT CTTATATGGA TTCGATAATA 
TTGAGCTTGG GTCAGCAGGA CTACAATTTG TTGAAGATAT GTTTGAACTA CCAAGATAAT 
ATAGGGAACA CTCCACTGCA TTTATCTGCT TTAAACTTGA ACTTCGAGGT TTACAACAGA 
CTGGTATATC TTGGTGCGTC AACAGATATA TTGAATCTAG ATAACGAATC GCCTGCGTCA 
ATAATGAATA AGTTTAATAC TCCGGCTGGC GGGTCAAACA GCAGAAACAA CAACACCAAG 
GCAGATCGAA AGTTAGCTCG AAACCTTCCT CAAAAGAATT ATTACCAACA ACAGCAACAG 
CAACAGCAAC CACAAAACAA TGTCAAAATT CCAAAAATTA TAAAAACTCA ACATCCTGAT 
AAAGAAGATT CTACTGCAGA TGTCAATATA GCTAAAACAG ATTCTGAAGT CAACGAAAGT 
CAATACCTGC ATTCCAACCA GCCAAATTCT ACAAATATGA ACACTATTAT GGAAGACTTG 
TCGAATATTA ATTCTTTCGT GACGTCGTCT GTCATTAAAG ATATAAAGTC TACGCCGTCC 
AAAATTCTTG AAAACTCTCC CATTTTATAC AGAAGAAGAT CACAGTCAAT ATCAGATGAA 
AAAGAAAAGG CAAAGGATAA TGAGAATCAA GTTGAAAAGA AAAAGGATCC CTTGAATAGT 
GTGAAAACTG CCATGCCATC ATTGGAATCG CCTTCTTCCC TTCTGCCGAT ACAGATGTCT 
CCACTAGGAA AGTACTCAAA ACCGCTTTCG CAGCAGATCA ACAAATTAAA TACAAAAGTT 
TCTTCTCTAC AAAGAATAAT GGGTGAAGAA ATTAAAAATC TGGACAACGA AGTAGTAGAA 
ACCGAATCCT CTATTTCTAA CAATAAGAAG AGATTGATTA CAATAGCACA TCAAATAGAG 
GATGCTTTCG ATTCCGTATC AAACAAAACT CCCATAAATT CGATATCAGA TTTACAATCA 
AGGATTAAAG AAACATCGTC AAAGTTGAAT TCTGAAAAGC AAAATTTTAT TCAAAGCTTG 
GAAAAATCTC AAGCTTTAAA ACTGGCTACA ATTGTCCAGG ATGAGGAATC AAAGGTTGAT 
ATGAATACTA ACTCAAGTTC ACATCCAGAA AAACAGGAAG ATGAAGAGCC AATTCCAAAG 
TCAACGTCGG AAACTTCTTC TCCTAAAAAC ACCAAAGCGG ACGCCAAATT TTCTAATACT 
GTACAAGAAT CATATGATGT AAATGAGACG TTGAGACTAG CAACGGAACT AACTATTCTA 
CAGTTTAAAA GAAGGATGAC AACATTGAAA ATAAGCGAAG CTAAAAGCAA AATTAATTCA 
TCGGTGAAGT TAGATAAATA CAGAAACTTG ATTGGTATCA CAATTGAGAA CATTGACTCA 
AAATTGGACG ATATAGAAAA GGATTTGAGG GCAAACGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011036.1
CDS	1..3282
Translation	MPFDVLISNQKDNTNHQNITPISKSVLLAPHSNHPVIEIATYSETDVYECYIRGFETKIVMRRTKDDWINITQVFKIAQFSKTKRTKILEKESNDMQHEKVQGGYGRFQGTWIPLDSAKFLVNKYEIIDPVVNSILTFQFDPNNPPPKRSKNSILRKTSPGTKITSPSSYNKTPRKKNSSSSTSATTTAANKKGKKNASINQPNPSPLQNLVFQTPQQFQVNSSMNIMNNNDNHTTMNFNNDTRHNLINNISNNSNQSTIIQQQKSIHENSFNNNYSATQKPLQFFPIPTNLQNKNVALNNPNNNDSNSYSHNIDNVINSSNNNNNGNNNNLIIVPDGPMQSQQQQQHHHEYLTNNFNHSMMDSITNGNSKKRRKKLNQSNEQQFYNQQEKIQRHFKLMKQPLLWQSFQNPNDHHNEYCDSNGSNNNNNTVASNGSSIEVFSSNENDNSMNMSSRSMTPFSAGNTSSQNKLENKMTDQEYKQTILTILSSERSSDVDQALLATLYPAPKNFNINFEIDDQGHTPLHWATAMANIPLIKMLITLNANALQCNKLGFNCITKSIFYNNCYKENAFDEIISILKICLITPDVNGRLPFHYLIELSVNKSKNPMIIKSYMDSIILSLGQQDYNLLKICLNYQDNIGNTPLHLSALNLNFEVYNRLVYLGASTDILNLDNESPASIMNKFNTPAGGSNSRNNNTKADRKLARNLPQKNYYQQQQQQQQPQNNVKIPKIIKTQHPDKEDSTADVNIAKTDSEVNESQYLHSNQPNSTNMNTIMEDLSNINSFVTSSVIKDIKSTPSKILENSPILYRRRSQSISDEKEKAKDNENQVEKKKDPLNSVKTAMPSLESPSSLLPIQMSPLGKYSKPLSQQINKLNTKVSSLQRIMGEEIKNLDNEVVETESSISNNKKRLITIAHQIEDAFDSVSNKTPINSISDLQSRIKETSSKLNSEKQNFIQSLEKSQALKLATIVQDEESKVDMNTNSSSHPEKQEDEEPIPKSTSETSSPKNTKADAKFSNTVQESYDVNETLRLATELTILQFKRRMTTLKISEAKSKINSSVKLDKYRNLIGITIENIDSKLDDIEKDLRANA

Target ORF information:

RefSeq Version	NM_001179001.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C SBF complex DNA-binding subunit SWI4 (SWI4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179001.1

ORF Insert Sequence:

ATGCCATTTG ATGTTTTGAT ATCAAACCAA AAAGATAATA CCAATCACCA GAACATCACG 
CCAATATCAA AATCAGTTCT TTTGGCGCCT CATTCAAACC ATCCAGTGAT TGAAATAGCT 
ACGTATTCAG AAACCGATGT ATACGAATGC TATATACGTG GGTTTGAAAC TAAAATAGTG 
ATGAGAAGAA CTAAAGATGA TTGGATTAAT ATTACCCAGG TTTTCAAAAT AGCTCAATTT 
TCGAAGACGA AAAGAACTAA AATACTGGAA AAGGAATCAA ATGATATGCA ACATGAAAAA 
GTTCAAGGTG GGTATGGTAG GTTTCAAGGT ACTTGGATCC CACTGGATTC AGCGAAGTTT 
TTAGTCAATA AGTACGAAAT AATTGACCCT GTGGTCAACT CCATCTTAAC TTTTCAATTC 
GATCCAAATA ACCCTCCACC AAAGAGAAGC AAAAATAGTA TATTGAGGAA GACTTCGCCG 
GGAACGAAGA TAACTTCTCC ATCGAGCTAC AATAAAACAC CAAGAAAAAA GAACAGTAGT 
TCATCAACAT CGGCTACAAC TACAGCTGCG AATAAGAAAG GCAAGAAAAA TGCATCGATC 
AACCAGCCGA ATCCAAGTCC TTTACAAAAC CTTGTTTTCC AAACTCCTCA ACAGTTCCAA 
GTAAATTCAT CAATGAATAT AATGAACAAT AATGATAACC ACACCACGAT GAATTTCAAC 
AATGACACAA GACACAATTT AATAAATAAT ATTAGTAACA ACAGCAATCA ATCTACTATT 
ATTCAACAAC AAAAATCCAT TCATGAAAAC TCTTTCAATA ATAATTATTC GGCAACTCAG 
AAACCTCTTC AATTTTTTCC AATTCCGACA AATTTACAAA ACAAGAACGT TGCTCTGAAT 
AACCCTAATA ATAATGACAG TAATAGTTAC AGCCATAACA TCGACAATGT CATTAACAGC 
AGTAATAACA ATAATAATGG CAATAATAAT AACTTGATCA TAGTTCCAGA CGGACCTATG 
CAAAGCCAAC AACAACAACA ACATCATCAT GAATATTTGA CGAATAACTT CAATCATTCC 
ATGATGGACT CCATTACAAA TGGCAATAGT AAAAAAAGGA GGAAAAAGCT GAATCAATCT 
AATGAACAAC AATTTTACAA CCAGCAAGAA AAAATACAAA GGCATTTCAA ATTAATGAAG 
CAACCCTTAT TATGGCAATC CTTTCAAAAT CCAAATGATC ACCATAATGA GTACTGTGAC 
AGTAACGGCA GCAACAATAA CAACAACACT GTAGCATCCA ATGGCTCCTC CATTGAAGTA 
TTTTCATCAA ATGAAAATGA TAACAGTATG AATATGTCTT CACGATCTAT GACACCGTTC 
AGCGCAGGAA ATACTTCATC TCAAAACAAG TTGGAAAACA AAATGACAGA TCAAGAATAC 
AAGCAGACGA TATTAACAAT ACTATCATCG GAAAGGTCTT CTGATGTAGA TCAAGCGTTG 
TTGGCTACTT TATATCCGGC GCCCAAAAAT TTTAACATAA ATTTTGAAAT TGATGATCAA 
GGTCACACTC CACTCCATTG GGCTACAGCT ATGGCGAATA TTCCTTTGAT AAAAATGCTA 
ATTACCTTAA ATGCTAATGC ATTACAGTGT AATAAGCTCG GTTTTAATTG CATCACAAAA 
TCAATTTTCT ACAACAATTG CTACAAGGAG AATGCTTTCG ATGAAATAAT ATCCATCTTA 
AAAATATGTC TTATAACTCC CGACGTAAAT GGAAGATTGC CATTTCATTA TTTAATTGAA 
TTGAGCGTTA ATAAGTCCAA AAATCCAATG ATAATAAAAT CTTATATGGA TTCGATAATA 
TTGAGCTTGG GTCAGCAGGA CTACAATTTG TTGAAGATAT GTTTGAACTA CCAAGATAAT 
ATAGGGAACA CTCCACTGCA TTTATCTGCT TTAAACTTGA ACTTCGAGGT TTACAACAGA 
CTGGTATATC TTGGTGCGTC AACAGATATA TTGAATCTAG ATAACGAATC GCCTGCGTCA 
ATAATGAATA AGTTTAATAC TCCGGCTGGC GGGTCAAACA GCAGAAACAA CAACACCAAG 
GCAGATCGAA AGTTAGCTCG AAACCTTCCT CAAAAGAATT ATTACCAACA ACAGCAACAG 
CAACAGCAAC CACAAAACAA TGTCAAAATT CCAAAAATTA TAAAAACTCA ACATCCTGAT 
AAAGAAGATT CTACTGCAGA TGTCAATATA GCTAAAACAG ATTCTGAAGT CAACGAAAGT 
CAATACCTGC ATTCCAACCA GCCAAATTCT ACAAATATGA ACACTATTAT GGAAGACTTG 
TCGAATATTA ATTCTTTCGT GACGTCGTCT GTCATTAAAG ATATAAAGTC TACGCCGTCC 
AAAATTCTTG AAAACTCTCC CATTTTATAC AGAAGAAGAT CACAGTCAAT ATCAGATGAA 
AAAGAAAAGG CAAAGGATAA TGAGAATCAA GTTGAAAAGA AAAAGGATCC CTTGAATAGT 
GTGAAAACTG CCATGCCATC ATTGGAATCG CCTTCTTCCC TTCTGCCGAT ACAGATGTCT 
CCACTAGGAA AGTACTCAAA ACCGCTTTCG CAGCAGATCA ACAAATTAAA TACAAAAGTT 
TCTTCTCTAC AAAGAATAAT GGGTGAAGAA ATTAAAAATC TGGACAACGA AGTAGTAGAA 
ACCGAATCCT CTATTTCTAA CAATAAGAAG AGATTGATTA CAATAGCACA TCAAATAGAG 
GATGCTTTCG ATTCCGTATC AAACAAAACT CCCATAAATT CGATATCAGA TTTACAATCA 
AGGATTAAAG AAACATCGTC AAAGTTGAAT TCTGAAAAGC AAAATTTTAT TCAAAGCTTG 
GAAAAATCTC AAGCTTTAAA ACTGGCTACA ATTGTCCAGG ATGAGGAATC AAAGGTTGAT 
ATGAATACTA ACTCAAGTTC ACATCCAGAA AAACAGGAAG ATGAAGAGCC AATTCCAAAG 
TCAACGTCGG AAACTTCTTC TCCTAAAAAC ACCAAAGCGG ACGCCAAATT TTCTAATACT 
GTACAAGAAT CATATGATGT AAATGAGACG TTGAGACTAG CAACGGAACT AACTATTCTA 
CAGTTTAAAA GAAGGATGAC AACATTGAAA ATAAGCGAAG CTAAAAGCAA AATTAATTCA 
TCGGTGAAGT TAGATAAATA CAGAAACTTG ATTGGTATCA CAATTGAGAA CATTGACTCA 
AAATTGGACG ATATAGAAAA GGATTTGAGG GCAAACGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SWI4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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