ORF » Species Summary » Saccharomyces cerevisiae S288C » SPF1 cDNA ORF clone

SPF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following SPF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi05458 NM_001178846.3
Latest version!		 Saccharomyces cerevisiae S288C ion-transporting P-type ATPase SPF1 (SPF1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPF1 ( NM_001178846.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010883.3 Saccharomyces cerevisiae S288C ion-transporting P-type ATPase SPF1 (SPF1), partial mRNA.
    Price & Availability↑
    CloneID OSi05458
    Clone ID Related Accession (Same CDS sequence) NM_001178846.3
    Accession Version NM_001178846.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product ion-transporting P-type ATPase SPF1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001178846.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACAAAGA AATCATTTGT GAGCAGTCCT ATTGTTAGGG ACTCGACATT GCTGGTACCA 
    AAGTCATTAA TAGCAAAGCC GTACGTATTA CCGTTTTTCC CGTTATACGC TACCTTTGCC 
    CAACTATATT TCCAGCAATA TGATCGTTAC ATCAAAGGAC CTGAGTGGAC TTTTGTCTAC 
    TTGGGGACAT TGGTATCCTT GAACATCCTA GTCATGTTAA TGCCTGCTTG GAATGTCAAG 
    ATCAAGGCGA AATTCAACTA CAGTACTACT AAAAATGTCA ATGAAGCTAC TCACATTCTC 
    ATCTACACCA CTCCAAATAA TGGTTCTGAT GGTATCGTTG AAATTCAGAG AGTAACTGAA 
    GCGGGCTCTT TGCAGACTTT TTTCCAATTT CAAAAGAAAA GATTCTTGTG GCATGAAAAT 
    GAGCAGGTAT TTTCTTCACC AAAGTTCCTT GTTGATGAAT CCCCAAAGAT CGGTGATTTC 
    CAAAAATGTA AGGGCCATTC CGGTGACCTA ACTCATTTGA AGAGACTATA TGGTGAGAAT 
    AGTTTTGATA TTCCTATTCC AACATTTATG GAATTATTCA AAGAGCACGC TGTTGCGCCA 
    TTGTTTGTTT TCCAAGTTTT CTGTGTTGCC TTATGGCTAT TGGATGAGTT CTGGTATTAT 
    TCTTTGTTTA ACTTATTCAT GATTATCTCC ATGGAGGCAG CTGCAGTATT TCAAAGATTA 
    ACTGCCTTGA AGGAATTTAG AACTATGGGT ATCAAACCTT ATACCATTAA CGTGTTTAGA 
    AACAAAAAAT GGGTAGCATT GCAAACCAAT GAATTGTTAC CTATGGATTT AGTGTCTATA 
    ACAAGAACCG CCGAGGAGAG CGCAATTCCT TGCGATTTGA TCTTGCTCGA TGGCTCCGCT 
    ATTGTTAATG AAGCAATGCT TTCAGGTGAA TCTACTCCCT TGTTAAAGGA ATCCATTAAA 
    TTACGTCCTA GTGAAGATAA TTTACAACTG GACGGTGTTG ACAAGATTGC TGTGTTACAT 
    GGTGGTACAA AGGCATTACA AGTAACACCT CCTGAACATA AATCGGATAT CCCTCCTCCA 
    CCTGATGGAG GTGCCTTAGC CATCGTCACC AAAACTGGCT TTGAAACATC TCAGGGTTCT 
    TTAGTTCGAG TTATGATTTA CTCCGCTGAA CGTGTTTCCG TCGATAATAA GGAAGCGTTG 
    ATGTTCATTC TTTTCTTGTT GATTTTCGCT GTTATTGCAT CCTGGTACGT TTGGGTTGAG 
    GGTACAAAAA TGGGTAGAAT TCAATCCAAA TTGATCTTGG ACTGTATATT AATTATTACA 
    TCTGTTGTTC CTCCTGAGTT ACCAATGGAA TTGACCATGG CTGTCAACAG TTCTCTGGCC 
    GCCCTTGCCA AGTTCTATGT CTATTGTACT GAACCATTTA GAATTCCATT TGCTGGTAGA 
    ATTGATGTTT GTTGTTTCGA TAAAACTGGT ACTTTGACCG GCGAAGATCT TGTGTTTGAA 
    GGTCTAGCTG GTATCTCTGC GGACTCCGAA AACATTCGTC ACTTGTACAG CGCTGCCGAA 
    GCACCAGAAA GTACAATTCT TGTCATTGGT GCTGCTCACG CTTTAGTTAA ATTGGAAGAT 
    GGTGATATTG TTGGTGACCC AATGGAAAAG GCGACCTTAA AGGCTGTTGG TTGGGCTGTC 
    GAAAGAAAAA ACAGTAACTA TAGAGAGGGC ACCGGAAAAT TGGATATTAT CCGTAGATTT 
    CAATTTTCTT CTGCTTTAAA AAGGTCTGCT TCTATTGCTT CTCACAACGA TGCCTTGTTT 
    GCTGCTGTCA AAGGTGCACC TGAAACGATT CGTGAAAGGC TTTCTGACAT TCCCAAGAAT 
    TATGACGAAA TCTATAAATC CTTTACACGC TCTGGTTCAA GAGTTTTGGC TTTGGCTTCT 
    AAGTCGTTGC CAAAAATGTC TCAGTCTAAA ATTGATGATC TAAACCGTGA TGATGTTGAA 
    TCTGAATTAA CCTTTAATGG GTTTTTGATT TTCCATTGTC CTCTGAAGGA TGACGCTATT 
    GAAACCATTA AAATGTTAAA TGAATCCTCC CATCGTTCTA TTATGATTAC TGGTGATAAT 
    CCATTAACTG CTGTTCATGT GGCTAAAGAA GTTGGCATAG TTTTTGGCGA AACTTTAATC 
    TTAGACAGAG CAGGTAAATC TGACGACAAT CAGTTGCTGT TCCGTGATGT TGAAGAGACA 
    GTTTCCATCC CATTTGATCC ATCAAAAGAC ACTTTTGATC ATTCTAAATT ATTTGATAGA 
    TATGATATTG CTGTTACTGG ATATGCTTTG AATGCCTTGG AAGGGCATTC TCAATTAAGG 
    GATCTATTAC GTCACACCTG GGTTTACGCA CGTGTTTCAC CATCCCAAAA GGAGTTCTTG 
    CTGAATACAC TAAAGGATAT GGGATATCAA ACTTTAATGT GCGGTGATGG TACTAATGAT 
    GTTGGTGCTT TGAAACAAGC TCATGTTGGT ATTGCATTGT TGAATGGTAC CGAAGAAGGT 
    TTGAAAAAAT TGGGTGAACA ACGCAGATTG GAAGGTATGA AAATGATGTA TATAAAGCAA 
    ACTGAATTTA TGGCTAGATG GAATCAACCA CAGCCACCTG TTCCTGAACC GATTGCACAC 
    TTATTCCCAC CTGGTCCAAA AAATCCTCAT TATCTGAAAG CTTTAGAAAG CAAAGGTACC 
    GTCATTACGC CAGAAATCAG AAAAGCAGTT GAAGAAGCAA ATTCCAAACC TGTTGAAGTC 
    ATCAAACCAA ATGGACTATC GGAAAAAAAA CCTGCTGACT TAGCTTCTTT GTTGTTGAAC 
    TCTGCTGGTG ATGCCCAAGG TGATGAAGCA CCAGCTCTAA AGTTAGGAGA TGCATCTTGC 
    GCTGCCCCTT TTACCTCGAA ACTGGCCAAT GTTTCTGCTG TTACTAACAT TATACGTCAA 
    GGCCGTTGTG CTTTAGTTAA CACAATTCAA ATGTACAAAA TCTTGGCCTT GAATTGTTTA 
    ATCAGTGCCT ACTCTTTGTC CATCATTTAC ATGGCTGGTG TCAAATTTGG TGATGGTCAA 
    GCCACTGTCT CCGGTTTGTT ACTATCGGTT TGTTTCTTGA GTATTTCCCG TGGTAAACCT 
    TTAGAAAAAC TATCCAAGCA AAGGCCACAG TCCGGAATTT TCAATGTTTA CATTATGGGA 
    TCTATTTTGT CTCAATTTGC CGTTCATATT GCCACTTTAG TTTACATCAC TACAGAGATT 
    TATAAATTGG AACCCAGAGA GCCACAAGTT GATCTTGAAA AGGAATTTGC TCCATCATTA 
    TTGAATACGG GTATTTTCAT CATTCAGTTG GTACAACAAG TCTCTACATT TGCTGTCAAT 
    TATCAAGGTG AGCCATTCAG AGAAAACATT AGGAGTAACA AAGGTATGTA TTATGGTTTA 
    TTGGGTGTGA CAGGTTTGGC ACTTGCAAGT GCTACTGAAT TTTTACCTGA GCTAAACGAA 
    GCCATGAAGT TTGTGCCAAT GACTGATGAC TTTAAGATTA AGTTGACCCT AACATTATTG 
    CTCGATTTCT TTGGTAGTTG GGGTGTCGAG CATTTTTTCA AGTTTTTCTT CATGGACGAC 
    AAACCATCAG ACATTTCTGT GCAACAGGTC AAGATTGCCT CTAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_010883.3
    CDS1..3648
    Translation

    Target ORF information:

    RefSeq Version NM_001178846.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C ion-transporting P-type ATPase SPF1 (SPF1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178846.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACAAAGA AATCATTTGT GAGCAGTCCT ATTGTTAGGG ACTCGACATT GCTGGTACCA 
    AAGTCATTAA TAGCAAAGCC GTACGTATTA CCGTTTTTCC CGTTATACGC TACCTTTGCC 
    CAACTATATT TCCAGCAATA TGATCGTTAC ATCAAAGGAC CTGAGTGGAC TTTTGTCTAC 
    TTGGGGACAT TGGTATCCTT GAACATCCTA GTCATGTTAA TGCCTGCTTG GAATGTCAAG 
    ATCAAGGCGA AATTCAACTA CAGTACTACT AAAAATGTCA ATGAAGCTAC TCACATTCTC 
    ATCTACACCA CTCCAAATAA TGGTTCTGAT GGTATCGTTG AAATTCAGAG AGTAACTGAA 
    GCGGGCTCTT TGCAGACTTT TTTCCAATTT CAAAAGAAAA GATTCTTGTG GCATGAAAAT 
    GAGCAGGTAT TTTCTTCACC AAAGTTCCTT GTTGATGAAT CCCCAAAGAT CGGTGATTTC 
    CAAAAATGTA AGGGCCATTC CGGTGACCTA ACTCATTTGA AGAGACTATA TGGTGAGAAT 
    AGTTTTGATA TTCCTATTCC AACATTTATG GAATTATTCA AAGAGCACGC TGTTGCGCCA 
    TTGTTTGTTT TCCAAGTTTT CTGTGTTGCC TTATGGCTAT TGGATGAGTT CTGGTATTAT 
    TCTTTGTTTA ACTTATTCAT GATTATCTCC ATGGAGGCAG CTGCAGTATT TCAAAGATTA 
    ACTGCCTTGA AGGAATTTAG AACTATGGGT ATCAAACCTT ATACCATTAA CGTGTTTAGA 
    AACAAAAAAT GGGTAGCATT GCAAACCAAT GAATTGTTAC CTATGGATTT AGTGTCTATA 
    ACAAGAACCG CCGAGGAGAG CGCAATTCCT TGCGATTTGA TCTTGCTCGA TGGCTCCGCT 
    ATTGTTAATG AAGCAATGCT TTCAGGTGAA TCTACTCCCT TGTTAAAGGA ATCCATTAAA 
    TTACGTCCTA GTGAAGATAA TTTACAACTG GACGGTGTTG ACAAGATTGC TGTGTTACAT 
    GGTGGTACAA AGGCATTACA AGTAACACCT CCTGAACATA AATCGGATAT CCCTCCTCCA 
    CCTGATGGAG GTGCCTTAGC CATCGTCACC AAAACTGGCT TTGAAACATC TCAGGGTTCT 
    TTAGTTCGAG TTATGATTTA CTCCGCTGAA CGTGTTTCCG TCGATAATAA GGAAGCGTTG 
    ATGTTCATTC TTTTCTTGTT GATTTTCGCT GTTATTGCAT CCTGGTACGT TTGGGTTGAG 
    GGTACAAAAA TGGGTAGAAT TCAATCCAAA TTGATCTTGG ACTGTATATT AATTATTACA 
    TCTGTTGTTC CTCCTGAGTT ACCAATGGAA TTGACCATGG CTGTCAACAG TTCTCTGGCC 
    GCCCTTGCCA AGTTCTATGT CTATTGTACT GAACCATTTA GAATTCCATT TGCTGGTAGA 
    ATTGATGTTT GTTGTTTCGA TAAAACTGGT ACTTTGACCG GCGAAGATCT TGTGTTTGAA 
    GGTCTAGCTG GTATCTCTGC GGACTCCGAA AACATTCGTC ACTTGTACAG CGCTGCCGAA 
    GCACCAGAAA GTACAATTCT TGTCATTGGT GCTGCTCACG CTTTAGTTAA ATTGGAAGAT 
    GGTGATATTG TTGGTGACCC AATGGAAAAG GCGACCTTAA AGGCTGTTGG TTGGGCTGTC 
    GAAAGAAAAA ACAGTAACTA TAGAGAGGGC ACCGGAAAAT TGGATATTAT CCGTAGATTT 
    CAATTTTCTT CTGCTTTAAA AAGGTCTGCT TCTATTGCTT CTCACAACGA TGCCTTGTTT 
    GCTGCTGTCA AAGGTGCACC TGAAACGATT CGTGAAAGGC TTTCTGACAT TCCCAAGAAT 
    TATGACGAAA TCTATAAATC CTTTACACGC TCTGGTTCAA GAGTTTTGGC TTTGGCTTCT 
    AAGTCGTTGC CAAAAATGTC TCAGTCTAAA ATTGATGATC TAAACCGTGA TGATGTTGAA 
    TCTGAATTAA CCTTTAATGG GTTTTTGATT TTCCATTGTC CTCTGAAGGA TGACGCTATT 
    GAAACCATTA AAATGTTAAA TGAATCCTCC CATCGTTCTA TTATGATTAC TGGTGATAAT 
    CCATTAACTG CTGTTCATGT GGCTAAAGAA GTTGGCATAG TTTTTGGCGA AACTTTAATC 
    TTAGACAGAG CAGGTAAATC TGACGACAAT CAGTTGCTGT TCCGTGATGT TGAAGAGACA 
    GTTTCCATCC CATTTGATCC ATCAAAAGAC ACTTTTGATC ATTCTAAATT ATTTGATAGA 
    TATGATATTG CTGTTACTGG ATATGCTTTG AATGCCTTGG AAGGGCATTC TCAATTAAGG 
    GATCTATTAC GTCACACCTG GGTTTACGCA CGTGTTTCAC CATCCCAAAA GGAGTTCTTG 
    CTGAATACAC TAAAGGATAT GGGATATCAA ACTTTAATGT GCGGTGATGG TACTAATGAT 
    GTTGGTGCTT TGAAACAAGC TCATGTTGGT ATTGCATTGT TGAATGGTAC CGAAGAAGGT 
    TTGAAAAAAT TGGGTGAACA ACGCAGATTG GAAGGTATGA AAATGATGTA TATAAAGCAA 
    ACTGAATTTA TGGCTAGATG GAATCAACCA CAGCCACCTG TTCCTGAACC GATTGCACAC 
    TTATTCCCAC CTGGTCCAAA AAATCCTCAT TATCTGAAAG CTTTAGAAAG CAAAGGTACC 
    GTCATTACGC CAGAAATCAG AAAAGCAGTT GAAGAAGCAA ATTCCAAACC TGTTGAAGTC 
    ATCAAACCAA ATGGACTATC GGAAAAAAAA CCTGCTGACT TAGCTTCTTT GTTGTTGAAC 
    TCTGCTGGTG ATGCCCAAGG TGATGAAGCA CCAGCTCTAA AGTTAGGAGA TGCATCTTGC 
    GCTGCCCCTT TTACCTCGAA ACTGGCCAAT GTTTCTGCTG TTACTAACAT TATACGTCAA 
    GGCCGTTGTG CTTTAGTTAA CACAATTCAA ATGTACAAAA TCTTGGCCTT GAATTGTTTA 
    ATCAGTGCCT ACTCTTTGTC CATCATTTAC ATGGCTGGTG TCAAATTTGG TGATGGTCAA 
    GCCACTGTCT CCGGTTTGTT ACTATCGGTT TGTTTCTTGA GTATTTCCCG TGGTAAACCT 
    TTAGAAAAAC TATCCAAGCA AAGGCCACAG TCCGGAATTT TCAATGTTTA CATTATGGGA 
    TCTATTTTGT CTCAATTTGC CGTTCATATT GCCACTTTAG TTTACATCAC TACAGAGATT 
    TATAAATTGG AACCCAGAGA GCCACAAGTT GATCTTGAAA AGGAATTTGC TCCATCATTA 
    TTGAATACGG GTATTTTCAT CATTCAGTTG GTACAACAAG TCTCTACATT TGCTGTCAAT 
    TATCAAGGTG AGCCATTCAG AGAAAACATT AGGAGTAACA AAGGTATGTA TTATGGTTTA 
    TTGGGTGTGA CAGGTTTGGC ACTTGCAAGT GCTACTGAAT TTTTACCTGA GCTAAACGAA 
    GCCATGAAGT TTGTGCCAAT GACTGATGAC TTTAAGATTA AGTTGACCCT AACATTATTG 
    CTCGATTTCT TTGGTAGTTG GGGTGTCGAG CATTTTTTCA AGTTTTTCTT CATGGACGAC 
    AAACCATCAG ACATTTCTGT GCAACAGGTC AAGATTGCCT CTAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
    Nature387(6632 Suppl)78-81(1997 May)
    Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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