MCM3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MCM3
Entrez Gene ID	856680
Full Name	MCM DNA helicase complex subunit MCM3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178847.1	NP_010882.1	MCM DNA helicase complex subunit MCM3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mcm3	NP_032589.1
Eremothecium gossypii	AGOS_AFR355C	NP_985902.2
Arabidopsis thaliana (thale cress)	MCM3	NP_199440.1
Homo sapiens (human)	MCM3	NP_002379.3
	MCM3	XP_001106966.1
Gallus gallus (chicken)	MCM3	NP_001006421.1
Rattus norvegicus (Norway rat)	Mcm3	NP_001178734.2
Saccharomyces cerevisiae (baker's yeast)	MCM3	NP_010882.1
Schizosaccharomyces pombe (fission yeast)	mcm3	NP_587795.1
Magnaporthe oryzae (rice blast fungus)	MGG_01759	XP_003714758.1
Oryza sativa (rice)	Os05g0476200	NP_001055835.1
Pan troglodytes (chimpanzee)	MCM3	XP_001151750.2
Canis lupus familiaris (dog)	MCM3	XP_538960.3
Danio rerio (zebrafish)	mcm3	NP_997732.1
Drosophila melanogaster (fruit fly)	Mcm3	NP_511048.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001135	XP_322026.2
Kluyveromyces lactis	KLLA0E22243g	XP_454957.1
Neurospora crassa	NCU08009	XP_962679.1
Xenopus tropicalis (tropical clawed frog)	zmcm3	NP_001039231.1
Bos taurus (cattle)	MCM3	NP_001013604.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Mcm ATPase active sites that flank Mcm2/5 (i.e. Mcm5/3 and 6/2) modulate the biochemical activities of the putative Mcm2/5 gate.
Title: The Saccharomyces cerevisiae Mcm6/2 and Mcm5/3 ATPase active sites contribute to the function of the putative Mcm2-7 'gate'.

DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA.
Title: Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.

The following MCM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05457	NM_001178847.1 Latest version!	Saccharomyces cerevisiae S288C MCM DNA helicase complex subunit MCM3 (MCM3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCM3 ( NM_001178847.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010882.1 Saccharomyces cerevisiae S288C MCM DNA helicase complex subunit MCM3 (MCM3), partial mRNA.

Price & Availability↑

CloneID	OSi05457
Clone ID Related Accession (Same CDS sequence)	NM_001178847.1
Accession Version	NM_001178847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	MCM DNA helicase complex subunit MCM3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAGGCT CAACGGGATT TGATGGAGAC GCTACTACTT TTTTCGCTCC AGACGCTGTG 
TTTGGTGACA GAGTGCGCAG ATTTCAAGAG TTTTTAGATA CTTTCACCTC ATACAGAGAC 
TCTGTAAGGT CCATACAAGT TTACAACAGC AATAACGCGG CCAACTACAA CGATGATCAA 
GATGACGCAG ACGAACGAGA TTTGCTAGGT GATGACGACG GTGATGATCT TGAAAAGGAA 
AAGAAAGCAG CATCGTCCAC CTCATTGAAT ATACTCCCTC ACAGGATTAT CATCTCGCTT 
GATGACTTGA GAGAATTCGA CAGGTCGTTC TGGTCGGGCA TTTTAGTCGA ACCAGCATAC 
TTCATCCCGC CTGCCGAAAA GGCGCTTACT GACCTAGCAG ATTCCATGGA CGATGTTCCA 
CATCCCAATG CCTCTGCAGT ATCGTCTCGC CATCCTTGGA AGCTTTCGTT CAAAGGCTCA 
TTTGGTGCAC ACGCATTGTC TCCTCGTACT CTAACGGCAC AACATTTAAA CAAACTGGTC 
TCTGTTGAGG GTATCGTAAC TAAGACTTCG TTGGTCAGGC CAAAGCTTAT CAGATCTGTC 
CACTACGCGG CAAAGACTGG TAGATTCCAT TACAGAGATT ATACAGATGC TACTACAACT 
CTCACCACCC GCATCCCAAC GCCTGCCATC TATCCAACGG AGGACACTGA AGGTAACAAA 
CTAACCACCG AATATGGGTA TAGTACGTTC ATAGACCATC AGCGTATCAC TGTGCAAGAA 
ATGCCCGAAA TGGCCCCCGC TGGCCAACTT CCCAGGTCCA TTGACGTCAT TCTCGATGAC 
GACCTTGTGG ACAAGACCAA GCCAGGTGAC AGAGTTAACG TTGTCGGGGT ATTCAAGTCG 
CTTGGTGCTG GTGGCATGAA CCAGTCCAAC TCTAATACAT TGATCGGGTT CAAAACTCTG 
ATCCTAGGTA ATACGGTGTA TCCTCTCCAC GCCAGATCCA CGGGTGTCGC TGCGAGACAA 
ATGTTGACAG ATTTCGATAT AAGAAATATC AATAAACTAT CCAAAAAAAA GGACATTTTC 
GATATCTTGT CTCAATCTTT AGCGCCTTCT ATTTATGGAC ATGACCATAT AAAGAAAGCC 
ATTTTATTGA TGCTCATGGG AGGTGTGGAG AAAAATTTAG AAAATGGCTC GCATTTAAGA 
GGTGACATCA ATATCCTAAT GGTGGGTGAT CCATCCACTG CCAAGTCCCA ATTGCTAAGG 
TTTGTGTTGA ATACAGCATC ACTGGCAATT GCTACTACTG GTAGAGGTTC TTCCGGTGTC 
GGTTTGACCG CAGCGGTCAC TACTGATAGG GAAACAGGTG AAAGAAGACT AGAGGCTGGT 
GCCATGGTTC TTGCTGACCG CGGGGTTGTA TGTATTGATG AATTTGATAA GATGACAGAT 
GTGGATAGAG TCGCCATTCA TGAAGTAATG GAACAACAAA CGGTGACGAT TGCCAAAGCA 
GGTATTCACA CAACATTAAA TGCTCGTTGT AGTGTTATTG CTGCCGCAAA TCCCGTTTTT 
GGGCAGTACG ATGTCAATAG AGATCCACAC CAAAACATTG CCCTACCGGA CTCGCTGTTG 
TCTCGTTTTG ATTTACTATT TGTTGTGACA GACGATATCA ATGAAATCAG AGATAGATCC 
ATTAGTGAGC ATGTCTTAAG AACACACAGA TATTTGCCTC CAGGTTATTT AGAGGGTGAA 
CCTGTGAGAG AGCGTTTGAA TTTATCATTA GCCGTTGGGG AGGATGCAGA TATAAATCCT 
GAAGAGCATT CCAACTCCGG GGCTGGTGTA GAAAATGAAG GAGAAGATGA TGAAGACCAT 
GTCTTCGAAA AGTTCAACCC CTTATTACAA GCAGGTGCTA AGTTAGCAAA AAACAAAGGT 
AACTATAACG GTACAGAAAT TCCAAAGCTA GTCACCATCC CATTCTTAAG AAAGTACGTT 
CAATATGCCA AGGAAAGGGT TATTCCACAG TTAACACAAG AAGCCATCAA TGTTATTGTG 
AAAAATTATA CTGATTTAAG AAACGATGAT AATACCAAAA AATCGCCCAT TACTGCAAGA 
ACTTTGGAGA CTTTGATCAG ATTAGCCACA GCTCACGCCA AAGTCAGGTT ATCCAAAACA 
GTCAACAAGG TGGATGCTAA AGTGGCTGCC AATCTACTAA GGTTTGCACT ATTGGGTGAG 
GATATCGGCA ATGATATCGA TGAAGAGGAA AGTGAATACG AAGAAGCTTT GTCGAAGAGG 
TCTCCACAGA AATCACCGAA AAAAAGACAA AGAGTCAGAC AACCAGCAAG CAACTCTGGA 
TCCCCAATCA AATCTACTCC AAGAAGGTCA ACGGCATCTT CCGTTAATGC CACGCCATCG 
TCAGCACGCA GAATATTACG TTTTCAAGAT GACGAACAGA ACGCTGGTGA AGACGATAAC 
GATATAATGT CACCGCTTCC TGCGGATGAG GAAGCTGAAT TACAAAGAAG GCTTCAACTG 
GGGTTGAGAG TGTCTCCAAG ACGTAGAGAA CATCTTCACG CACCTGAGGA AGGTTCGTCG 
GGACCTCTTA CCGAGGTCGG TACTCCAAGA TTACCTAACG TATCTTCTGC AGGTCAGGAT 
GATGAGCAAC AACAGTCAGT TATTTCTTTT GACAATGTGG AGCCTGGTAC CATTTCTACT 
GGTAGATTGT CTTTAATCTC AGGTATTATT GCGCGTCTGA TGCAAACAGA AATATTTGAA 
GAAGAATCCT ATCCTGTGGC CTCTTTGTTC GAAAGAATCA ACGAAGAACT ACCGGAGGAG 
GAAAAATTCT CCGCTCAAGA ATATTTAGCA GGTTTGAAGA TCATGTCGGA CAGAAATAAC 
TTAATGGTTG CTGACGATAA AGTTTGGAGA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010882.1
CDS	1..2916
Translation	MEGSTGFDGDATTFFAPDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNDDQDDADERDLLGDDDGDDLEKEKKAASSTSLNILPHRIIISLDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHPNASAVSSRHPWKLSFKGSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAKTGRFHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITVQEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQSNSNTLIGFKTLILGNTVYPLHARSTGVAARQMLTDFDIRNINKLSKKKDIFDILSQSLAPSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQLLRFVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADRGVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAANPVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHVLRTHRYLPPGYLEGEPVRERLNLSLAVGEDADINPEEHSNSGAGVENEGEDDEDHVFEKFNPLLQAGAKLAKNKGNYNGTEIPKLVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPITARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALLGEDIGNDIDEEESEYEEALSKRSPQKSPKKRQRVRQPASNSGSPIKSTPRRSTASSVNATPSSARRILRFQDDEQNAGEDDNDIMSPLPADEEAELQRRLQLGLRVSPRRREHLHAPEEGSSGPLTEVGTPRLPNVSSAGQDDEQQQSVISFDNVEPGTISTGRLSLISGIIARLMQTEIFEEESYPVASLFERINEELPEEEKFSAQEYLAGLKIMSDRNNLMVADDKVWRV

Target ORF information:

RefSeq Version	NM_001178847.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C MCM DNA helicase complex subunit MCM3 (MCM3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178847.1

ORF Insert Sequence:

ATGGAAGGCT CAACGGGATT TGATGGAGAC GCTACTACTT TTTTCGCTCC AGACGCTGTG 
TTTGGTGACA GAGTGCGCAG ATTTCAAGAG TTTTTAGATA CTTTCACCTC ATACAGAGAC 
TCTGTAAGGT CCATACAAGT TTACAACAGC AATAACGCGG CCAACTACAA CGATGATCAA 
GATGACGCAG ACGAACGAGA TTTGCTAGGT GATGACGACG GTGATGATCT TGAAAAGGAA 
AAGAAAGCAG CATCGTCCAC CTCATTGAAT ATACTCCCTC ACAGGATTAT CATCTCGCTT 
GATGACTTGA GAGAATTCGA CAGGTCGTTC TGGTCGGGCA TTTTAGTCGA ACCAGCATAC 
TTCATCCCGC CTGCCGAAAA GGCGCTTACT GACCTAGCAG ATTCCATGGA CGATGTTCCA 
CATCCCAATG CCTCTGCAGT ATCGTCTCGC CATCCTTGGA AGCTTTCGTT CAAAGGCTCA 
TTTGGTGCAC ACGCATTGTC TCCTCGTACT CTAACGGCAC AACATTTAAA CAAACTGGTC 
TCTGTTGAGG GTATCGTAAC TAAGACTTCG TTGGTCAGGC CAAAGCTTAT CAGATCTGTC 
CACTACGCGG CAAAGACTGG TAGATTCCAT TACAGAGATT ATACAGATGC TACTACAACT 
CTCACCACCC GCATCCCAAC GCCTGCCATC TATCCAACGG AGGACACTGA AGGTAACAAA 
CTAACCACCG AATATGGGTA TAGTACGTTC ATAGACCATC AGCGTATCAC TGTGCAAGAA 
ATGCCCGAAA TGGCCCCCGC TGGCCAACTT CCCAGGTCCA TTGACGTCAT TCTCGATGAC 
GACCTTGTGG ACAAGACCAA GCCAGGTGAC AGAGTTAACG TTGTCGGGGT ATTCAAGTCG 
CTTGGTGCTG GTGGCATGAA CCAGTCCAAC TCTAATACAT TGATCGGGTT CAAAACTCTG 
ATCCTAGGTA ATACGGTGTA TCCTCTCCAC GCCAGATCCA CGGGTGTCGC TGCGAGACAA 
ATGTTGACAG ATTTCGATAT AAGAAATATC AATAAACTAT CCAAAAAAAA GGACATTTTC 
GATATCTTGT CTCAATCTTT AGCGCCTTCT ATTTATGGAC ATGACCATAT AAAGAAAGCC 
ATTTTATTGA TGCTCATGGG AGGTGTGGAG AAAAATTTAG AAAATGGCTC GCATTTAAGA 
GGTGACATCA ATATCCTAAT GGTGGGTGAT CCATCCACTG CCAAGTCCCA ATTGCTAAGG 
TTTGTGTTGA ATACAGCATC ACTGGCAATT GCTACTACTG GTAGAGGTTC TTCCGGTGTC 
GGTTTGACCG CAGCGGTCAC TACTGATAGG GAAACAGGTG AAAGAAGACT AGAGGCTGGT 
GCCATGGTTC TTGCTGACCG CGGGGTTGTA TGTATTGATG AATTTGATAA GATGACAGAT 
GTGGATAGAG TCGCCATTCA TGAAGTAATG GAACAACAAA CGGTGACGAT TGCCAAAGCA 
GGTATTCACA CAACATTAAA TGCTCGTTGT AGTGTTATTG CTGCCGCAAA TCCCGTTTTT 
GGGCAGTACG ATGTCAATAG AGATCCACAC CAAAACATTG CCCTACCGGA CTCGCTGTTG 
TCTCGTTTTG ATTTACTATT TGTTGTGACA GACGATATCA ATGAAATCAG AGATAGATCC 
ATTAGTGAGC ATGTCTTAAG AACACACAGA TATTTGCCTC CAGGTTATTT AGAGGGTGAA 
CCTGTGAGAG AGCGTTTGAA TTTATCATTA GCCGTTGGGG AGGATGCAGA TATAAATCCT 
GAAGAGCATT CCAACTCCGG GGCTGGTGTA GAAAATGAAG GAGAAGATGA TGAAGACCAT 
GTCTTCGAAA AGTTCAACCC CTTATTACAA GCAGGTGCTA AGTTAGCAAA AAACAAAGGT 
AACTATAACG GTACAGAAAT TCCAAAGCTA GTCACCATCC CATTCTTAAG AAAGTACGTT 
CAATATGCCA AGGAAAGGGT TATTCCACAG TTAACACAAG AAGCCATCAA TGTTATTGTG 
AAAAATTATA CTGATTTAAG AAACGATGAT AATACCAAAA AATCGCCCAT TACTGCAAGA 
ACTTTGGAGA CTTTGATCAG ATTAGCCACA GCTCACGCCA AAGTCAGGTT ATCCAAAACA 
GTCAACAAGG TGGATGCTAA AGTGGCTGCC AATCTACTAA GGTTTGCACT ATTGGGTGAG 
GATATCGGCA ATGATATCGA TGAAGAGGAA AGTGAATACG AAGAAGCTTT GTCGAAGAGG 
TCTCCACAGA AATCACCGAA AAAAAGACAA AGAGTCAGAC AACCAGCAAG CAACTCTGGA 
TCCCCAATCA AATCTACTCC AAGAAGGTCA ACGGCATCTT CCGTTAATGC CACGCCATCG 
TCAGCACGCA GAATATTACG TTTTCAAGAT GACGAACAGA ACGCTGGTGA AGACGATAAC 
GATATAATGT CACCGCTTCC TGCGGATGAG GAAGCTGAAT TACAAAGAAG GCTTCAACTG 
GGGTTGAGAG TGTCTCCAAG ACGTAGAGAA CATCTTCACG CACCTGAGGA AGGTTCGTCG 
GGACCTCTTA CCGAGGTCGG TACTCCAAGA TTACCTAACG TATCTTCTGC AGGTCAGGAT 
GATGAGCAAC AACAGTCAGT TATTTCTTTT GACAATGTGG AGCCTGGTAC CATTTCTACT 
GGTAGATTGT CTTTAATCTC AGGTATTATT GCGCGTCTGA TGCAAACAGA AATATTTGAA 
GAAGAATCCT ATCCTGTGGC CTCTTTGTTC GAAAGAATCA ACGAAGAACT ACCGGAGGAG 
GAAAAATTCT CCGCTCAAGA ATATTTAGCA GGTTTGAAGA TCATGTCGGA CAGAAATAAC 
TTAATGGTTG CTGACGATAA AGTTTGGAGA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MCM3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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