ORF » Species Summary » Saccharomyces cerevisiae S288C » AFG1 cDNA ORF clone

AFG1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following AFG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi05438 NM_001178867.1
Latest version!		 Saccharomyces cerevisiae S288C Afg1p (AFG1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    AFG1 ( NM_001178867.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010862.1 Saccharomyces cerevisiae S288C Afg1p (AFG1), partial mRNA.
    Price & Availability↑
    CloneID OSi05438
    Clone ID Related Accession (Same CDS sequence) NM_001178867.1
    Accession Version NM_001178867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Afg1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATCGCTT TGAAGCCCAA TGCTGTTCGA ACCTTCCGAC AAGTGCAGCA TTGCAGCTTT 
    CGCATTTGTC GGTATCAATC TACGAAGTCA AATAAGTGTC TGACGCCCTT GCAAGAGTAC 
    GACAGACTGG TGAAGTTGGG GAAGCTACGG GATGATACAT ATCAGCGTGG TATCATCTCT 
    TCCTTAGGGG ATTTGTATGA TTCACTGGTT AAATATGTAC CTCCGGTTGT CAAGACACCC 
    AATGCTGTCG ACCAAGTTGG CGGTTGGTTG AATGGTCTTA AATCCGTATT TAGCCGTGGC 
    AAACCTAAGA ACATTGGGGC GTATGTGGAT GTATCCAAAA TTGGTAACTC GATACCTCGA 
    GGAGTTTACC TATATGGAGA TGTTGGCTGC GGAAAGACAA TGTTGATGGA CCTTTTTTAT 
    ACTACAATTC CCAATCATTT AACAAAAAAG AGAATACATT TTCACCAGTT TATGCAATAT 
    GTTCACAAAA GGTCGCATGA AATTGTTAGA GAGCAAAATT TGAAAGAACT AGGTGATGCA 
    AAAGGAAAAG AGATCGATAC GGTTCCATTT TTGGCCGCAG AGATTGCAAA TAATTCGCAT 
    GTTCTTTGTT TTGACGAGTT TCAAGTCACT GACGTGGCAG ATGCAATGAT ATTGAGAAGG 
    CTGATGACTG CCTTACTATC CGATGATTAT GGTGTCGTAC TTTTCGCAAC CTCGAATAGA 
    CATCCAGATG AGTTGTATAT CAACGGTGTT CAAAGACAAT CATTTATTCC TTGTATTGAA 
    CTGATAAAGC ATAGAACTAA GGTTATCTTC TTGAATTCGC CAACAGATTA CCGTAAGATT 
    CCAAGACCTG TGTCCTCAGT TTACTATTTC CCATCCGATA CGAGCATAAA ATACGCATCA 
    AAGGAATGTA AAACCCGTCG AGAAACTCAT ATTAAGGAAT GGTATAACTA TTTCGCACAG 
    GCTTCCCACA CCGATGATTC CACTGATTCA CACACGGTGC ATAAGACATT TTATGATTAT 
    CCATTAACTA TTTGGGGGAG AGAGTTCAAA GTCCCTAAGT GTACCCCACC TCGAGTCGCG 
    CAGTTTACTT TCAAGCAGTT GTGTGGTGAG CCTTTGGCCG CAGGAGATTA CTTGACGTTG 
    GCAAAAAATT TTGAAGCCTT TATAGTGACC GATATTCCAT ATTTGTCCAT TTACGTTCGT 
    GATGAAGTGA GAAGATTTAT TACATTTTTA GATGCTGTAT ATGACAGTGG CGGGAAACTG 
    GCCACTACGG GTGCAGCGGA TTTTTCTTCC TTGTTTGTGG AACCTGAACA GATACTTAAT 
    GATTTTGAGT TACGCCCAAC AACCAAAGAA CCTGATAGCG TCGATACTGG TATGGTAGAT 
    GAGATGGTTG AGAAACACGG TTTTTCGAAA GAGATTGCCA AGAAATCGCA GATGTTTGCT 
    CTTGATGAAG AAAGATTTGC CTTTGCCCGT GCCTTAAGCA GGCTGTCACA GATGAGCTCC 
    ACCGATTGGG TTACTAAGCC TACATACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_010862.1
    CDS1..1530
    Translation

    Target ORF information:

    RefSeq Version NM_001178867.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Afg1p (AFG1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGATCGCTT TGAAGCCCAA TGCTGTTCGA ACCTTCCGAC AAGTGCAGCA TTGCAGCTTT 
    CGCATTTGTC GGTATCAATC TACGAAGTCA AATAAGTGTC TGACGCCCTT GCAAGAGTAC 
    GACAGACTGG TGAAGTTGGG GAAGCTACGG GATGATACAT ATCAGCGTGG TATCATCTCT 
    TCCTTAGGGG ATTTGTATGA TTCACTGGTT AAATATGTAC CTCCGGTTGT CAAGACACCC 
    AATGCTGTCG ACCAAGTTGG CGGTTGGTTG AATGGTCTTA AATCCGTATT TAGCCGTGGC 
    AAACCTAAGA ACATTGGGGC GTATGTGGAT GTATCCAAAA TTGGTAACTC GATACCTCGA 
    GGAGTTTACC TATATGGAGA TGTTGGCTGC GGAAAGACAA TGTTGATGGA CCTTTTTTAT 
    ACTACAATTC CCAATCATTT AACAAAAAAG AGAATACATT TTCACCAGTT TATGCAATAT 
    GTTCACAAAA GGTCGCATGA AATTGTTAGA GAGCAAAATT TGAAAGAACT AGGTGATGCA 
    AAAGGAAAAG AGATCGATAC GGTTCCATTT TTGGCCGCAG AGATTGCAAA TAATTCGCAT 
    GTTCTTTGTT TTGACGAGTT TCAAGTCACT GACGTGGCAG ATGCAATGAT ATTGAGAAGG 
    CTGATGACTG CCTTACTATC CGATGATTAT GGTGTCGTAC TTTTCGCAAC CTCGAATAGA 
    CATCCAGATG AGTTGTATAT CAACGGTGTT CAAAGACAAT CATTTATTCC TTGTATTGAA 
    CTGATAAAGC ATAGAACTAA GGTTATCTTC TTGAATTCGC CAACAGATTA CCGTAAGATT 
    CCAAGACCTG TGTCCTCAGT TTACTATTTC CCATCCGATA CGAGCATAAA ATACGCATCA 
    AAGGAATGTA AAACCCGTCG AGAAACTCAT ATTAAGGAAT GGTATAACTA TTTCGCACAG 
    GCTTCCCACA CCGATGATTC CACTGATTCA CACACGGTGC ATAAGACATT TTATGATTAT 
    CCATTAACTA TTTGGGGGAG AGAGTTCAAA GTCCCTAAGT GTACCCCACC TCGAGTCGCG 
    CAGTTTACTT TCAAGCAGTT GTGTGGTGAG CCTTTGGCCG CAGGAGATTA CTTGACGTTG 
    GCAAAAAATT TTGAAGCCTT TATAGTGACC GATATTCCAT ATTTGTCCAT TTACGTTCGT 
    GATGAAGTGA GAAGATTTAT TACATTTTTA GATGCTGTAT ATGACAGTGG CGGGAAACTG 
    GCCACTACGG GTGCAGCGGA TTTTTCTTCC TTGTTTGTGG AACCTGAACA GATACTTAAT 
    GATTTTGAGT TACGCCCAAC AACCAAAGAA CCTGATAGCG TCGATACTGG TATGGTAGAT 
    GAGATGGTTG AGAAACACGG TTTTTCGAAA GAGATTGCCA AGAAATCGCA GATGTTTGCT 
    CTTGATGAAG AAAGATTTGC CTTTGCCCGT GCCTTAAGCA GGCTGTCACA GATGAGCTCC 
    ACCGATTGGG TTACTAAGCC TACATACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
    Nature387(6632 Suppl)78-81(1997 May)
    Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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