SCH9 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SCH9
Entrez Gene ID	856612
Full Name	serine/threonine protein kinase SCH9
Synonyms	HRM2,KOM1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179336.1	NP_012075.1	serine/threonine protein kinase SCH9

Pathways from BioSystems

Homologies

Homo sapiens (human)	RPS6KB2	NP_003943.2
Pan troglodytes (chimpanzee)	RPS6KB2	XP_001172909.1
	RPS6KB2	XP_001117937.1
Rattus norvegicus (Norway rat)	Rps6kb2	NP_001010962.2
Saccharomyces cerevisiae (baker's yeast)	SCH9	NP_012075.1
Eremothecium gossypii	AGOS_ADL389W	NP_983707.1
Bos taurus (cattle)	RPS6KB2	NP_001192511.1
Kluyveromyces lactis	KLLA0B03586g	XP_451694.1
Canis lupus familiaris (dog)	RPS6KB2	XP_851971.1
Mus musculus (house mouse)	Rps6kb2	NP_067460.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show that the role of Sch9 in regulating ageing is tightly connected with V-ATPase activity and vacuolar acidity. As both Sch9 and the V-ATPase are highly conserved in higher eukaryotes, it will be interesting to further clarify their cooperative action on the cellular processes that influence growth and ageing.
Title: The yeast protein kinase Sch9 adjusts V-ATPase assembly/disassembly to control pH homeostasis and longevity in response to glucose availability.

We also show that Tor1p vacuolar localization and TORC1 anabolic functions, including growth promotion and phosphorylation of its direct substrate Sch9, are compromised in s-hhy mutants. Thus, an intact trans-Golgi and late endosome interface is a requisite for efficient Tor1 vacuolar localization and TORC1 function.
Title: Hygromycin B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function.

SCH9 deletion causes the upregulation of many amino acid biosynthesis pathways and increases glycerol during fermentation. When Gcn4p was overexpressed during winemaking, increased glycerol production was also observed.
Title: Sch9p kinase and the Gcn4p transcription factor regulate glycerol production during winemaking.

Thus, Sch9 is a Cst6/Rca1 kinase that links CO2 adaptation to lipid signaling via Pkh1/2 in fungi.
Title: Lipid Signaling via Pkh1/2 Regulates Fungal CO2 Sensing through the Kinase Sch9.

Overexpressing SCH9, but not TOR1, allows newly-formed tetraploids to exhibit evolved phenotypes and knocking out SCH9 diminishes the evolved phenotypes. Furthermore, when cells were challenged with conditions causing ancestral cells to evolve aneuploidy, tetraploidy was maintained in the evolved lines. Our results reveal a determinant role for Sch9 during the early stage of polyploid evolution.
Title: Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast.

The following SCH9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCH9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05400	NM_001179336.1 Latest version!	Saccharomyces cerevisiae S288C serine/threonine protein kinase SCH9 (SCH9), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCH9 ( NM_001179336.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012075.1 Saccharomyces cerevisiae S288C serine/threonine protein kinase SCH9 (SCH9), partial mRNA.

Price & Availability↑

CloneID	OSi05400
Clone ID Related Accession (Same CDS sequence)	NM_001179336.1
Accession Version	NM_001179336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	serine/threonine protein kinase SCH9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATGAATT TTTTTACATC AAAATCGTCG AATCAGGATA CTGGATTTAG CTCTCAACAC 
CAACATCCAA ATGGACAGAA CAATGGAAAC AATAATAGCA GCACCGCTGG CAACGACAAC 
GGATACCCAT GTAAACTGGT GTCCAGTGGG CCCTGCGCTT CATCAAATAA TGGTGCCCTT 
TTTACGAATT TTACTTTACA AACTGCAACG CCGACCACCG CTATTAGTCA GGACTTATAT 
GCAATGGGCA CAACAGGAAT AACATCAGAA AATGCCCTTT TTCAAATGAA GTCAATGAAT 
AATGGAATAT CATCAGTTAA TAATAACAAC AGCAACACCC CTACGATTAT TACCACGTCA 
CAGGAAGAAA CTAATGCTGG AAATGTACAT GGCGATACCG GTGGCAATTC TTTGCAAAAT 
TCTGAAGATG ACAACTTTTC TTCCAGTTCT ACCACCAAAT GCTTACTCTC TTCCACTTCT 
TCGCTATCAA TAAATCAACG AGAAGCAGCA GCAGCTGCTT ATGGTCCAGA TACCGATATT 
CCTAGGGGTA AACTAGAAGT TACAATAATA GAAGCACGTG ACCTAGTCAC TAGATCAAAG 
GATTCACAGC CTTATGTTGT TTGTACTTTT GAGAGTTCAG AGTTCATTTC TAATGGTCCT 
GAGTCACTAG GCGCCATTAA TAATAACAAC AATAACAACA ACAATAATCA GCATAATCAA 
AACCAGCATA TTAACAACAA CAACGAAAAT ACCAACCCTG ACGCTGCTAG CCAGCATCAT 
AATAATAACA GTGGTTGGAA CGGTTCTCAG TTACCATCGA TAAAAGAGCA CTTGAAGAAA 
AAACCCCTTT ATACACACAG ATCATCTTCC CAATTAGATC AGCTAAACTC TTGCTCTTCA 
GTAACCGATC CGAGCAAACG TTCTTCTAAT TCTTCGTCGG GTTCTTCAAA TGGTCCAAAG 
AATGATAGTT CACATCCAAT ATGGCATCAC AAGACAACGT TTGATGTTTT GGGATCTCAC 
TCGGAATTAG ATATTTCTGT TTATGATGCT GCCCACGACC ATATGTTCTT AGGCCAAGTT 
AGACTGTATC CAATGATTCA TAATTTAGCA CATGCTTCCC AACACCAATG GCACAGTTTG 
AAACCTCGCG TTATTGATGA AGTTGTGTCC GGTGATATTT TAATCAAATG GACTTACAAA 
CAGACAAAGA AAAGACATTA TGGCCCACAA GATTTTGAAG TTCTTCGATT ATTGGGTAAG 
GGTACTTTTG GCCAAGTCTA CCAAGTTAAG AAGAAAGACA CTCAAAGAAT TTATGCAATG 
AAAGTTCTCT CCAAGAAAGT TATTGTCAAG AAAAATGAGA TCGCCCACAC AATTGGCGAA 
AGAAATATCC TAGTCACGAC AGCGTCCAAA TCGTCCCCAT TCATTGTCGG ATTGAAGTTT 
TCCTTTCAAA CACCAACAGA TCTGTATTTG GTCACTGATT ATATGAGTGG TGGAGAATTA 
TTCTGGCATT TACAAAAGGA GGGCCGTTTT TCGGAAGACA GAGCGAAATT CTATATCGCT 
GAGTTAGTCC TAGCGTTAGA ACATTTACAC GATAACGATA TCGTTTACAG GGACCTAAAG 
CCTGAAAACA TTCTACTCGA TGCCAACGGT AACATCGCTC TTTGCGATTT TGGTCTTTCT 
AAAGCTGACT TGAAGGATAG AACAAACACA TTTTGCGGCA CCACGGAATA CCTGGCACCA 
GAATTGTTAC TGGACGAAAC CGGCTACACC AAAATGGTCG ATTTCTGGTC TCTAGGTGTT 
TTGATATTTG AAATGTGTTG TGGTTGGTCC CCTTTCTTTG CGGAAAATAA TCAAAAAATG 
TACCAAAAAA TTGCCTTTGG TAAAGTCAAA TTCCCCAGAG ACGTACTGTC ACAAGAGGGG 
AGGTCTTTTG TAAAGGGTTT ACTAAACAGA AACCCCAAAC ATAGACTCGG TGCCATTGAT 
GATGGAAGAG AACTACGAGC TCATCCATTT TTCGCAGATA TCGACTGGGA GGCCTTGAAG 
CAGAAAAAAA TTCCACCACC TTTCAAACCT CACCTAGTCT CGGAGACGGA TACCTCGAAT 
TTTGACCCAG AGTTCACAAC AGCTTCAACT TCATACATGA ACAAGCACCA GCCGATGATG 
ACTGCTACCC CGCTATCTCC AGCCATGCAA GCAAAGTTTG CTGGTTTCAC CTTTGTTGAT 
GAGTCCGCCA TCGATGAACA CGTTAATAAC AACAGAAAAT TCCTACAAAA CTCGTACTTT 
ATGGAACCTG GTTCCTTTAT CCCGGGAAAT CCAAACTTAC CTCCAGACGA AGATGTCATC 
GATGATGACG GGGACGAGGA CATCAATGAT GGATTCAACC AAGAGAAAAA TATGAACAAC 
AGCCATTCGC AGATGGACTT CGACGGCGAC CAACACATGG ATGACGAATT TGTCAGTGGA 
AGATTCGAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012075.1
CDS	1..2475
Translation	MMNFFTSKSSNQDTGFSSQHQHPNGQNNGNNNSSTAGNDNGYPCKLVSSGPCASSNNGALFTNFTLQTATPTTAISQDLYAMGTTGITSENALFQMKSMNNGISSVNNNNSNTPTIITTSQEETNAGNVHGDTGGNSLQNSEDDNFSSSSTTKCLLSSTSSLSINQREAAAAAYGPDTDIPRGKLEVTIIEARDLVTRSKDSQPYVVCTFESSEFISNGPESLGAINNNNNNNNNNQHNQNQHINNNNENTNPDAASQHHNNNSGWNGSQLPSIKEHLKKKPLYTHRSSSQLDQLNSCSSVTDPSKRSSNSSSGSSNGPKNDSSHPIWHHKTTFDVLGSHSELDISVYDAAHDHMFLGQVRLYPMIHNLAHASQHQWHSLKPRVIDEVVSGDILIKWTYKQTKKRHYGPQDFEVLRLLGKGTFGQVYQVKKKDTQRIYAMKVLSKKVIVKKNEIAHTIGERNILVTTASKSSPFIVGLKFSFQTPTDLYLVTDYMSGGELFWHLQKEGRFSEDRAKFYIAELVLALEHLHDNDIVYRDLKPENILLDANGNIALCDFGLSKADLKDRTNTFCGTTEYLAPELLLDETGYTKMVDFWSLGVLIFEMCCGWSPFFAENNQKMYQKIAFGKVKFPRDVLSQEGRSFVKGLLNRNPKHRLGAIDDGRELRAHPFFADIDWEALKQKKIPPPFKPHLVSETDTSNFDPEFTTASTSYMNKHQPMMTATPLSPAMQAKFAGFTFVDESAIDEHVNNNRKFLQNSYFMEPGSFIPGNPNLPPDEDVIDDDGDEDINDGFNQEKNMNNSHSQMDFDGDQHMDDEFVSGRFEI

Target ORF information:

RefSeq Version	NM_001179336.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C serine/threonine protein kinase SCH9 (SCH9), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179336.1

ORF Insert Sequence:

ATGATGAATT TTTTTACATC AAAATCGTCG AATCAGGATA CTGGATTTAG CTCTCAACAC 
CAACATCCAA ATGGACAGAA CAATGGAAAC AATAATAGCA GCACCGCTGG CAACGACAAC 
GGATACCCAT GTAAACTGGT GTCCAGTGGG CCCTGCGCTT CATCAAATAA TGGTGCCCTT 
TTTACGAATT TTACTTTACA AACTGCAACG CCGACCACCG CTATTAGTCA GGACTTATAT 
GCAATGGGCA CAACAGGAAT AACATCAGAA AATGCCCTTT TTCAAATGAA GTCAATGAAT 
AATGGAATAT CATCAGTTAA TAATAACAAC AGCAACACCC CTACGATTAT TACCACGTCA 
CAGGAAGAAA CTAATGCTGG AAATGTACAT GGCGATACCG GTGGCAATTC TTTGCAAAAT 
TCTGAAGATG ACAACTTTTC TTCCAGTTCT ACCACCAAAT GCTTACTCTC TTCCACTTCT 
TCGCTATCAA TAAATCAACG AGAAGCAGCA GCAGCTGCTT ATGGTCCAGA TACCGATATT 
CCTAGGGGTA AACTAGAAGT TACAATAATA GAAGCACGTG ACCTAGTCAC TAGATCAAAG 
GATTCACAGC CTTATGTTGT TTGTACTTTT GAGAGTTCAG AGTTCATTTC TAATGGTCCT 
GAGTCACTAG GCGCCATTAA TAATAACAAC AATAACAACA ACAATAATCA GCATAATCAA 
AACCAGCATA TTAACAACAA CAACGAAAAT ACCAACCCTG ACGCTGCTAG CCAGCATCAT 
AATAATAACA GTGGTTGGAA CGGTTCTCAG TTACCATCGA TAAAAGAGCA CTTGAAGAAA 
AAACCCCTTT ATACACACAG ATCATCTTCC CAATTAGATC AGCTAAACTC TTGCTCTTCA 
GTAACCGATC CGAGCAAACG TTCTTCTAAT TCTTCGTCGG GTTCTTCAAA TGGTCCAAAG 
AATGATAGTT CACATCCAAT ATGGCATCAC AAGACAACGT TTGATGTTTT GGGATCTCAC 
TCGGAATTAG ATATTTCTGT TTATGATGCT GCCCACGACC ATATGTTCTT AGGCCAAGTT 
AGACTGTATC CAATGATTCA TAATTTAGCA CATGCTTCCC AACACCAATG GCACAGTTTG 
AAACCTCGCG TTATTGATGA AGTTGTGTCC GGTGATATTT TAATCAAATG GACTTACAAA 
CAGACAAAGA AAAGACATTA TGGCCCACAA GATTTTGAAG TTCTTCGATT ATTGGGTAAG 
GGTACTTTTG GCCAAGTCTA CCAAGTTAAG AAGAAAGACA CTCAAAGAAT TTATGCAATG 
AAAGTTCTCT CCAAGAAAGT TATTGTCAAG AAAAATGAGA TCGCCCACAC AATTGGCGAA 
AGAAATATCC TAGTCACGAC AGCGTCCAAA TCGTCCCCAT TCATTGTCGG ATTGAAGTTT 
TCCTTTCAAA CACCAACAGA TCTGTATTTG GTCACTGATT ATATGAGTGG TGGAGAATTA 
TTCTGGCATT TACAAAAGGA GGGCCGTTTT TCGGAAGACA GAGCGAAATT CTATATCGCT 
GAGTTAGTCC TAGCGTTAGA ACATTTACAC GATAACGATA TCGTTTACAG GGACCTAAAG 
CCTGAAAACA TTCTACTCGA TGCCAACGGT AACATCGCTC TTTGCGATTT TGGTCTTTCT 
AAAGCTGACT TGAAGGATAG AACAAACACA TTTTGCGGCA CCACGGAATA CCTGGCACCA 
GAATTGTTAC TGGACGAAAC CGGCTACACC AAAATGGTCG ATTTCTGGTC TCTAGGTGTT 
TTGATATTTG AAATGTGTTG TGGTTGGTCC CCTTTCTTTG CGGAAAATAA TCAAAAAATG 
TACCAAAAAA TTGCCTTTGG TAAAGTCAAA TTCCCCAGAG ACGTACTGTC ACAAGAGGGG 
AGGTCTTTTG TAAAGGGTTT ACTAAACAGA AACCCCAAAC ATAGACTCGG TGCCATTGAT 
GATGGAAGAG AACTACGAGC TCATCCATTT TTCGCAGATA TCGACTGGGA GGCCTTGAAG 
CAGAAAAAAA TTCCACCACC TTTCAAACCT CACCTAGTCT CGGAGACGGA TACCTCGAAT 
TTTGACCCAG AGTTCACAAC AGCTTCAACT TCATACATGA ACAAGCACCA GCCGATGATG 
ACTGCTACCC CGCTATCTCC AGCCATGCAA GCAAAGTTTG CTGGTTTCAC CTTTGTTGAT 
GAGTCCGCCA TCGATGAACA CGTTAATAAC AACAGAAAAT TCCTACAAAA CTCGTACTTT 
ATGGAACCTG GTTCCTTTAT CCCGGGAAAT CCAAACTTAC CTCCAGACGA AGATGTCATC 
GATGATGACG GGGACGAGGA CATCAATGAT GGATTCAACC AAGAGAAAAA TATGAACAAC 
AGCCATTCGC AGATGGACTT CGACGGCGAC CAACACATGG ATGACGAATT TGTCAGTGGA 
AGATTCGAAA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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SCH9 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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