RPN10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RPN10
Entrez Gene ID	856607
Full Name	proteasome regulatory particle base subunit RPN10
Synonyms	MCB1,SUN1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179331.3	NP_012070.3	proteasome regulatory particle base subunit RPN10

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC100857577	XP_003642739.1
Canis lupus familiaris (dog)	PSMD4	NP_001130036.1
Mus musculus (house mouse)	Psmd4	NP_001268946.1
Danio rerio (zebrafish)	psmd4a	NP_001002112.1
Drosophila melanogaster (fruit fly)	Pros54	NP_001262168.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005559	XP_315562.4
Eremothecium gossypii	AGOS_ABL078C	NP_982869.1
Pan troglodytes (chimpanzee)	PSMD4	NP_001131029.1
Bos taurus (cattle)	PSMD4	NP_001013616.2
Gallus gallus (chicken)	PSMD4	NP_001135862.1
Saccharomyces cerevisiae (baker's yeast)	RPN10	NP_012070.3
Kluyveromyces lactis	KLLA0B03916g	XP_451708.1
Magnaporthe oryzae (rice blast fungus)	MGG_04506	XP_003710949.1
Homo sapiens (human)	PSMD4	NP_002801.1
	PSMD4	NP_001130040.1
Neurospora crassa	NCU02982	XP_965368.1
Oryza sativa (rice)	Os03g0243300	NP_001049527.1
Rattus norvegicus (Norway rat)	Psmd4	NP_112621.1
Caenorhabditis elegans (roundworm)	rpn-10	NP_492809.1
Schizosaccharomyces pombe (fission yeast)	rpn10	NP_594628.1
Arabidopsis thaliana (thale cress)	RPN10	NP_195575.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Genetic, bioinformatics, biochemical and biophysical data show that attraction between this alpha1-conjugated ubiquitin and the HECT ubiquitin-binding patch pulls the alpha1-helix out of the interface, thereby promoting trimerization. Strikingly, trimerization renders the ubiquitin ligase inactive.
Title: Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases.

functional yeast proteasomes have the capacity to associate and dissociate with Rpn10 and that Rpn10 monoubiquitination decreases the Rpn10-proteasome and Rpn10-Dsk2 associations.
Title: Rpn10 monoubiquitination orchestrates the association of the ubiquilin-type DSK2 receptor with the proteasome.

Mutations in this disordered region promote ubiquitin chain extension of Rpn10.
Title: An intrinsically disordered region of RPN10 plays a key role in restricting ubiquitin chain elongation in RPN10 monoubiquitination.

X-ray analysis revealed that these crystals of Rpn10 belonged to the monoclinic system C2, with unit-cell parameters a = 107.3, b = 49.7, c = 81.3 A, alpha = gamma = 90.0, beta = 130.5 degrees
Title: Purification and crystallization of mono-ubiquitylated ubiquitin receptor Rpn10.

On the basis of the mutual positions of Rpn10 and Rpn13, we propose a model for polyubiquitin binding to the 26S proteasome
Title: Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy.

The following RPN10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPN10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05395	NM_001179331.3 Latest version!	Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN10 (RPN10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPN10 ( NM_001179331.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012070.3 Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN10 (RPN10), partial mRNA.

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CloneID	OSi05395
Clone ID Related Accession (Same CDS sequence)	NM_001179331.3
Accession Version	NM_001179331.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	proteasome regulatory particle base subunit RPN10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). On Jul 30, 2012 this sequence version replaced NM_001179331.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTATTGG AAGCTACAGT GTTAGTGATT GATAATTCAG AGTACTCTCG CAATGGCGAC 
TTTCCTAGGA CAAGGTTTGA AGCCCAGATC GACTCGGTAG AGTTCATATT TCAAGCCAAG 
AGAAACAGCA ATCCTGAGAA TACAGTGGGT CTTATCTCTG GTGCTGGCGC CAACCCCAGG 
GTGTTATCTA CGTTTACCGC CGAGTTTGGG AAGATTCTTG CTGGACTACA CGACACGCAG 
ATCGAGGGTA AGCTGCATAT GGCCACTGCG TTGCAGATCG CTCAGCTGAC TTTGAAGCAT 
CGCCAGAATA AGGTCCAACA TCAAAGGATT GTGGCATTTG TGTGTAGCCC AATAAGTGAT 
TCTCGAGACG AATTGATCAG ATTGGCAAAA ACACTGAAAA AGAATAATGT TGCCGTGGAC 
ATCATCAATT TTGGAGAGAT TGAACAGAAC ACGGAGCTTT TGGATGAGTT CATAGCTGCA 
GTGAACAACC CTCAAGAAGA AACTAGTCAT TTGCTTACTG TGACGCCTGG CCCCAGACTG 
CTGTACGAGA ACATCGCATC TTCACCCATA ATTCTCGAAG AAGGATCCTC CGGTATGGGC 
GCCTTTGGTG GGTCTGGCGG TGATTCCGAT GCCAATGGCA CATTTATGGA CTTCGGGGTA 
GACCCATCAA TGGACCCAGA ACTGGCAATG GCCTTGCGTC TGTCTATGGA AGAAGAGCAG 
CAAAGACAGG AAAGGTTAAG ACAGCAGCAA CAACAACAAG ATCAGCCTGA GCAGTCTGAA 
CAGCCTGAAC AACACCAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012070.3
CDS	1..807
Translation	MVLEATVLVIDNSEYSRNGDFPRTRFEAQIDSVEFIFQAKRNSNPENTVGLISGAGANPRVLSTFTAEFGKILAGLHDTQIEGKLHMATALQIAQLTLKHRQNKVQHQRIVAFVCSPISDSRDELIRLAKTLKKNNVAVDIINFGEIEQNTELLDEFIAAVNNPQEETSHLLTVTPGPRLLYENIASSPIILEEGSSGMGAFGGSGGDSDANGTFMDFGVDPSMDPELAMALRLSMEEEQQRQERLRQQQQQQDQPEQSEQPEQHQDK

Target ORF information:

RefSeq Version	NM_001179331.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN10 (RPN10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179331.3

ORF Insert Sequence:

ATGGTATTGG AAGCTACAGT GTTAGTGATT GATAATTCAG AGTACTCTCG CAATGGCGAC 
TTTCCTAGGA CAAGGTTTGA AGCCCAGATC GACTCGGTAG AGTTCATATT TCAAGCCAAG 
AGAAACAGCA ATCCTGAGAA TACAGTGGGT CTTATCTCTG GTGCTGGCGC CAACCCCAGG 
GTGTTATCTA CGTTTACCGC CGAGTTTGGG AAGATTCTTG CTGGACTACA CGACACGCAG 
ATCGAGGGTA AGCTGCATAT GGCCACTGCG TTGCAGATCG CTCAGCTGAC TTTGAAGCAT 
CGCCAGAATA AGGTCCAACA TCAAAGGATT GTGGCATTTG TGTGTAGCCC AATAAGTGAT 
TCTCGAGACG AATTGATCAG ATTGGCAAAA ACACTGAAAA AGAATAATGT TGCCGTGGAC 
ATCATCAATT TTGGAGAGAT TGAACAGAAC ACGGAGCTTT TGGATGAGTT CATAGCTGCA 
GTGAACAACC CTCAAGAAGA AACTAGTCAT TTGCTTACTG TGACGCCTGG CCCCAGACTG 
CTGTACGAGA ACATCGCATC TTCACCCATA ATTCTCGAAG AAGGATCCTC CGGTATGGGC 
GCCTTTGGTG GGTCTGGCGG TGATTCCGAT GCCAATGGCA CATTTATGGA CTTCGGGGTA 
GACCCATCAA TGGACCCAGA ACTGGCAATG GCCTTGCGTC TGTCTATGGA AGAAGAGCAG 
CAAAGACAGG AAAGGTTAAG ACAGCAGCAA CAACAACAAG ATCAGCCTGA GCAGTCTGAA 
CAGCCTGAAC AACACCAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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RPN10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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