UBA4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	UBA4
Entrez Gene ID	856511
Full Name	Uba4p
Synonyms	YHR1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179241.1	NP_011979.1	Uba4p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	UBA4	NP_011979.1
Kluyveromyces lactis	KLLA0E21363g	XP_454917.1
Xenopus tropicalis (tropical clawed frog)	LOC100491723	XP_002935865.2
Mus musculus (house mouse)	Mocs3	NP_001153802.1
Danio rerio (zebrafish)	mocs3	NP_956421.1
Drosophila melanogaster (fruit fly)	CG13090	NP_001260255.1
Caenorhabditis elegans (roundworm)	moc-3	NP_501359.1
Magnaporthe oryzae (rice blast fungus)	MGG_05569	XP_003710444.1
Neurospora crassa	NCU00736	XP_964389.1
Arabidopsis thaliana (thale cress)	CNX5	NP_200324.1
Oryza sativa (rice)	Os02g0525400	NP_001046992.1
Homo sapiens (human)	MOCS3	NP_055299.1
Gallus gallus (chicken)	MOCS3	XP_001232656.1
Pan troglodytes (chimpanzee)	MOCS3	XP_514724.2
Rattus norvegicus (Norway rat)	Mocs3	NP_001101274.1
Eremothecium gossypii	AGOS_AER248W	NP_985105.1
Schizosaccharomyces pombe (fission yeast)	SPAC2G11.10c	NP_593090.1
	MOCS3	XP_001093903.1
Canis lupus familiaris (dog)	MOCS3	XP_543052.2
Anopheles gambiae (African malaria mosquito)	MOCS3_ANOGA	XP_321349.4

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ubiquitin-like Urm1.Uba4 systems are conserved and exchangeable between human and yeast cells
Title: Urmylation and tRNA thiolation functions of ubiquitin-like Uba4?Urm1 systems are conserved from yeast to man.

ubiquitin-like protein Urm1 and ubiquitin-activating enzyme-like protein Uba4, as well as Tuc1 and Tuc2, were strictly required for tRNA thio-modification
Title: Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems.

The Uba4-Urm1 system represents the evolutionary link between protein conjugation and protein modification by sulfur carrier proteins.
Title: The sulfurtransferase activity of Uba4 presents a link between ubiquitin-like protein conjugation and activation of sulfur carrier proteins.

The following UBA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05309	NM_001179241.1 Latest version!	Saccharomyces cerevisiae S288C Uba4p (UBA4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBA4 ( NM_001179241.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011979.1 Saccharomyces cerevisiae S288C Uba4p (UBA4), partial mRNA.

Price & Availability↑

CloneID	OSi05309
Clone ID Related Accession (Same CDS sequence)	NM_001179241.1
Accession Version	NM_001179241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Uba4p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAATGACT ACCATCTCGA GGATACCACG TCTGAACTTG AAGCATTAAG ATTGGAGAAT 
GCACAATTAA GAGAGCAGCT TGCCAAGAGA GAAGACAGCA GCCGCGACTA CCCATTATCT 
TTGGAAGAAT ACCAACGTTA CGGAAGACAA ATGATTGTTG AAGAAACAGG TGGTGTAGCA 
GGTCAAGTCA AGTTGAAAAA TACAAAAGTT TTGGTAGTTG GTGCTGGAGG TTTGGGATGT 
CCCGCCTTGC CCTACTTAGC GGGCGCTGGC GTGGGCCAAA TAGGCATAGT AGATAACGAT 
GTGGTGGAGA CTTCCAATTT GCATAGGCAG GTTCTTCATG ATTCTAGCAG AGTTGGAATG 
TTGAAATGTG AGTCGGCCAG GCAATATATC ACGAAACTGA ACCCACACAT TAACGTCGTT 
ACCTATCCCG TTAGATTGAA CTCCAGTAAT GCTTTTGACA TTTTCAAAGG TTACAATTAT 
ATATTAGACT GCACAGATTC TCCATTAACG AGATACCTGG TGTCTGATGT TGCAGTTAAT 
CTGGGAATAA CAGTAGTGTC TGCATCCGGT TTGGGAACAG AGGGCCAGCT AACTATATTG 
AATTTTAACA ATATAGGGCC ATGCTACAGA TGCTTTTATC CAACACCTCC GCCACCAAAT 
GCCGTGACCT CTTGCCAAGA AGGCGGTGTG ATAGGACCTT GCATTGGACT AGTTGGAACA 
ATGATGGCTG TAGAAACTTT GAAGCTTATC CTAGGAATCT ACACCAATGA AAATTTTAGT 
CCCTTTTTGA TGTTATATTC AGGTTTCCCA CAGCAGAGCC TGCGCACTTT CAAAATGAGA 
GGCAGACAAG AAAAGTGTCT ATGCTGTGGT AAGAATCGAA CGATAACAAA AGAAGCCATC 
GAAAAAGGTG AGATCAATTA CGAACTGTTT TGTGGCGCAC GAAACTATAA TGTATGCGAG 
CCTGATGAGA GAATCAGTGT GGACGCATTT CAGCGTATCT ACAAGGATGA TGAATTTCTA 
GCAAAACATA TATTTCTTGA TGTTAGGCCA TCCCATCATT ACGAGATATC TCATTTCCCT 
GAAGCAGTTA ATATTCCAAT CAAAAATCTA AGAGATATGA ACGGTGATCT TAAGAAACTG 
CAAGAAAAAC TTCCCAGTGT AGAAAAAGAC AGTAATATAG TGATTCTTTG CCGCTACGGT 
AACGACTCTC AGCTAGCTAC AAGATTATTG AAGGATAAAT TTGGGTTTTC TAATGTACGA 
GACGTGAGAG GAGGATACTT CAAATACATA GACGATATTG ATCAAACCAT TCCTAAATAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011979.1
CDS	1..1323
Translation	MNDYHLEDTTSELEALRLENAQLREQLAKREDSSRDYPLSLEEYQRYGRQMIVEETGGVAGQVKLKNTKVLVVGAGGLGCPALPYLAGAGVGQIGIVDNDVVETSNLHRQVLHDSSRVGMLKCESARQYITKLNPHINVVTYPVRLNSSNAFDIFKGYNYILDCTDSPLTRYLVSDVAVNLGITVVSASGLGTEGQLTILNFNNIGPCYRCFYPTPPPPNAVTSCQEGGVIGPCIGLVGTMMAVETLKLILGIYTNENFSPFLMLYSGFPQQSLRTFKMRGRQEKCLCCGKNRTITKEAIEKGEINYELFCGARNYNVCEPDERISVDAFQRIYKDDEFLAKHIFLDVRPSHHYEISHFPEAVNIPIKNLRDMNGDLKKLQEKLPSVEKDSNIVILCRYGNDSQLATRLLKDKFGFSNVRDVRGGYFKYIDDIDQTIPKY

Target ORF information:

RefSeq Version	NM_001179241.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Uba4p (UBA4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179241.1

ORF Insert Sequence:

ATGAATGACT ACCATCTCGA GGATACCACG TCTGAACTTG AAGCATTAAG ATTGGAGAAT 
GCACAATTAA GAGAGCAGCT TGCCAAGAGA GAAGACAGCA GCCGCGACTA CCCATTATCT 
TTGGAAGAAT ACCAACGTTA CGGAAGACAA ATGATTGTTG AAGAAACAGG TGGTGTAGCA 
GGTCAAGTCA AGTTGAAAAA TACAAAAGTT TTGGTAGTTG GTGCTGGAGG TTTGGGATGT 
CCCGCCTTGC CCTACTTAGC GGGCGCTGGC GTGGGCCAAA TAGGCATAGT AGATAACGAT 
GTGGTGGAGA CTTCCAATTT GCATAGGCAG GTTCTTCATG ATTCTAGCAG AGTTGGAATG 
TTGAAATGTG AGTCGGCCAG GCAATATATC ACGAAACTGA ACCCACACAT TAACGTCGTT 
ACCTATCCCG TTAGATTGAA CTCCAGTAAT GCTTTTGACA TTTTCAAAGG TTACAATTAT 
ATATTAGACT GCACAGATTC TCCATTAACG AGATACCTGG TGTCTGATGT TGCAGTTAAT 
CTGGGAATAA CAGTAGTGTC TGCATCCGGT TTGGGAACAG AGGGCCAGCT AACTATATTG 
AATTTTAACA ATATAGGGCC ATGCTACAGA TGCTTTTATC CAACACCTCC GCCACCAAAT 
GCCGTGACCT CTTGCCAAGA AGGCGGTGTG ATAGGACCTT GCATTGGACT AGTTGGAACA 
ATGATGGCTG TAGAAACTTT GAAGCTTATC CTAGGAATCT ACACCAATGA AAATTTTAGT 
CCCTTTTTGA TGTTATATTC AGGTTTCCCA CAGCAGAGCC TGCGCACTTT CAAAATGAGA 
GGCAGACAAG AAAAGTGTCT ATGCTGTGGT AAGAATCGAA CGATAACAAA AGAAGCCATC 
GAAAAAGGTG AGATCAATTA CGAACTGTTT TGTGGCGCAC GAAACTATAA TGTATGCGAG 
CCTGATGAGA GAATCAGTGT GGACGCATTT CAGCGTATCT ACAAGGATGA TGAATTTCTA 
GCAAAACATA TATTTCTTGA TGTTAGGCCA TCCCATCATT ACGAGATATC TCATTTCCCT 
GAAGCAGTTA ATATTCCAAT CAAAAATCTA AGAGATATGA ACGGTGATCT TAAGAAACTG 
CAAGAAAAAC TTCCCAGTGT AGAAAAAGAC AGTAATATAG TGATTCTTTG CCGCTACGGT 
AACGACTCTC AGCTAGCTAC AAGATTATTG AAGGATAAAT TTGGGTTTTC TAATGTACGA 
GACGTGAGAG GAGGATACTT CAAATACATA GACGATATTG ATCAAACCAT TCCTAAATAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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UBA4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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