PRE2 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Data suggest regulated process for removal of proteasomes upon nitrogen starvation; autophagy involves separate processes for dissociation and nuclear export of core (PRE2) and regulatory (RPN1) proteins; only core protein autophagy depends on UBP3.
Title: Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles.

Deletion of the N-terminal half of the beta5 propeptide causes substantial defects in proteasome assembly.
Title: Distinct Elements in the Proteasomal ?5 Subunit Propeptide Required for Autocatalytic Processing and Proteasome Assembly.

Data indicate that the dynamic gate can be opened by allosteric coupling to a tetrahedral transition state at any of the working active centers of proteasome.
Title: A tetrahedral transition state at the active sites of the 20S proteasome is coupled to opening of the alpha-ring channel.