ORF » Species Summary » Saccharomyces cerevisiae S288C » TIM50 cDNA ORF clone

TIM50 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following TIM50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIM50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04906 NM_001183877.1
Latest version!		 Saccharomyces cerevisiae S288C protein translocase subunit TIM50 (TIM50), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    TIM50 ( NM_001183877.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015262.1 Saccharomyces cerevisiae S288C protein translocase subunit TIM50 (TIM50), partial mRNA.
    Price & Availability↑
    CloneID OSi04906
    Clone ID Related Accession (Same CDS sequence) NM_001183877.1
    Accession Version NM_001183877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1431bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product protein translocase subunit TIM50
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTGTCCA TTTTAAGAAA TTCGGTGAGA CTAAATTCAA GGGCTCTTAG GGTTGTGCCA 
    TCTGCCGCCA ACACCTTAAC ATCGGTTCAA GCATCCAGAA GACTTTTAAC AAGTTATTCA 
    AGTTTTTTAC AAAAAGAAAC AAAAGACGAC AAGCCTAAAT CCATTCTTAC AGATGATATG 
    CTGTTCAAGG CCGGTGTTGA CGTCGATGAG AAGGGCCAAG GCAAAAACGA GGAAACATCA 
    GGAGAAGGAG GAGAGGACAA GAATGAACCT TCTTCCAAAA GTGAAAAATC TAGAAGAAAA 
    AGACAAACTT CTACAGATAT TAAAAGAGAA AAGTATGCTA ACTGGTTTTA CATTTTTTCG 
    TTGTCTGCGT TGACAGGTAC TGCAATCTAC ATGGCAAGGG ATTGGGAGCC TCAAGAGTCT 
    GAAGAATTGA AGAAAGACAT CGATAATGGC TACACTTTAT CACTTATGTA TAAAAGATTC 
    AAGGCCAGGT TCAACTCAAT GTTCACCTAC TTCCAAGAGC CACCTTTCCC TGATTTACTA 
    CCTCCACCAC CACCACCACC GTACCAAAGG CCATTAACTC TTGTTATCAC ATTGGAAGAT 
    TTTTTGGTTC ATTCTGAGTG GTCTCAAAAG CATGGTTGGA GAACGGCCAA AAGACCTGGT 
    GCTGACTACT TCTTGGGTTA CCTATCGCAG TATTACGAAA TTGTTTTGTT TTCATCCAAC 
    TATATGATGT ACTCTGACAA AATCGCTGAA AAATTAGATC CAATCCATGC ATTCGTATCT 
    TATAATTTGT TCAAGGAACA CTGTGTTTAC AAAGACGGTG TGCACATTAA GGATCTGTCA 
    AAATTGAATA GAGATTTGAG TAAAGTAATC ATTATTGACA CTGACCCTAA CAGTTACAAA 
    TTGCAACCTG AAAATGCTAT TCCAATGGAG CCATGGAATG GTGAAGCTGA TGACAAATTA 
    GTAAGATTGA TTCCATTTTT GGAGTACCTT GCCACTCAAC AAACCAAGGA TGTTAGACCA 
    ATCTTGAACA GCTTTGAAGA CAAGAAGAAC CTAGCAGAAG AATTTGATCA TCGTGTGAAA 
    AAATTGAAGG ATAAATTTTA CGGAGATCAT AAATCTGGTG GCAACTGGGC AATGACGGCA 
    CTAGGTCTCG GAAATTCCCT GGGCGGCAGC ACCAAGTTCC CGCTCGATTT GATTCATGAA 
    GAAGGACAAA AGAACTATTT AATGTTCATG AAGATGATTG AGGAAGAAAA GGAAAAAATT 
    AGAATACAGC AGGAGCAAAT GGGCGGGCAA ACATTTACGC TGAAAGACTA TGTTGAAGGT 
    AACTTGCCTT CGCCAGAAGA ACAAATGAAA ATACAATTGG AGAAGCAGAA GGAGGTAGAC 
    GCCTTATTTG AAGAGGAAAA GAAAAAGAAG AAGATTGCTG AATCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_015262.1
    CDS1..1431
    Translation

    Target ORF information:

    RefSeq Version NM_001183877.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C protein translocase subunit TIM50 (TIM50), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001183877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTGTCCA TTTTAAGAAA TTCGGTGAGA CTAAATTCAA GGGCTCTTAG GGTTGTGCCA 
    TCTGCCGCCA ACACCTTAAC ATCGGTTCAA GCATCCAGAA GACTTTTAAC AAGTTATTCA 
    AGTTTTTTAC AAAAAGAAAC AAAAGACGAC AAGCCTAAAT CCATTCTTAC AGATGATATG 
    CTGTTCAAGG CCGGTGTTGA CGTCGATGAG AAGGGCCAAG GCAAAAACGA GGAAACATCA 
    GGAGAAGGAG GAGAGGACAA GAATGAACCT TCTTCCAAAA GTGAAAAATC TAGAAGAAAA 
    AGACAAACTT CTACAGATAT TAAAAGAGAA AAGTATGCTA ACTGGTTTTA CATTTTTTCG 
    TTGTCTGCGT TGACAGGTAC TGCAATCTAC ATGGCAAGGG ATTGGGAGCC TCAAGAGTCT 
    GAAGAATTGA AGAAAGACAT CGATAATGGC TACACTTTAT CACTTATGTA TAAAAGATTC 
    AAGGCCAGGT TCAACTCAAT GTTCACCTAC TTCCAAGAGC CACCTTTCCC TGATTTACTA 
    CCTCCACCAC CACCACCACC GTACCAAAGG CCATTAACTC TTGTTATCAC ATTGGAAGAT 
    TTTTTGGTTC ATTCTGAGTG GTCTCAAAAG CATGGTTGGA GAACGGCCAA AAGACCTGGT 
    GCTGACTACT TCTTGGGTTA CCTATCGCAG TATTACGAAA TTGTTTTGTT TTCATCCAAC 
    TATATGATGT ACTCTGACAA AATCGCTGAA AAATTAGATC CAATCCATGC ATTCGTATCT 
    TATAATTTGT TCAAGGAACA CTGTGTTTAC AAAGACGGTG TGCACATTAA GGATCTGTCA 
    AAATTGAATA GAGATTTGAG TAAAGTAATC ATTATTGACA CTGACCCTAA CAGTTACAAA 
    TTGCAACCTG AAAATGCTAT TCCAATGGAG CCATGGAATG GTGAAGCTGA TGACAAATTA 
    GTAAGATTGA TTCCATTTTT GGAGTACCTT GCCACTCAAC AAACCAAGGA TGTTAGACCA 
    ATCTTGAACA GCTTTGAAGA CAAGAAGAAC CTAGCAGAAG AATTTGATCA TCGTGTGAAA 
    AAATTGAAGG ATAAATTTTA CGGAGATCAT AAATCTGGTG GCAACTGGGC AATGACGGCA 
    CTAGGTCTCG GAAATTCCCT GGGCGGCAGC ACCAAGTTCC CGCTCGATTT GATTCATGAA 
    GAAGGACAAA AGAACTATTT AATGTTCATG AAGATGATTG AGGAAGAAAA GGAAAAAATT 
    AGAATACAGC AGGAGCAAAT GGGCGGGCAA ACATTTACGC TGAAAGACTA TGTTGAAGGT 
    AACTTGCCTT CGCCAGAAGA ACAAATGAAA ATACAATTGG AGAAGCAGAA GGAGGTAGAC 
    GCCTTATTTG AAGAGGAAAA GAAAAAGAAG AAGATTGCTG AATCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
    Nature387(6632 Suppl)103-5(1997 May)
    Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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