ELP3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ELP3
Entrez Gene ID	856019
Full Name	Elongator subunit ELP3
Synonyms	HPA1,KAT9,KTI8,TOT3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183900.1	NP_015239.1	Elongator subunit ELP3

Pathways from BioSystems

Homologies

	ELP3	XP_001110226.1
Xenopus tropicalis (tropical clawed frog)	elp3	NP_001001215.1
Rattus norvegicus (Norway rat)	LOC100362548	XP_002728336.1
Eremothecium gossypii	AGOS_AGR201C	NP_986867.1
Schizosaccharomyces pombe (fission yeast)	elp3	NP_594862.1
Oryza sativa (rice)	Os04g0484900	NP_001053128.1
Pan troglodytes (chimpanzee)	ELP3	XP_001165532.1
Mus musculus (house mouse)	Elp3	NP_083087.1
Gallus gallus (chicken)	ELP3	NP_001034382.1
Drosophila melanogaster (fruit fly)	Elp3	NP_608834.1
Danio rerio (zebrafish)	elp3	NP_001014344.2
Caenorhabditis elegans (roundworm)	elpc-3	NP_506055.2
Saccharomyces cerevisiae (baker's yeast)	ELP3	NP_015239.1
Homo sapiens (human)	ELP3	NP_060561.3
Canis lupus familiaris (dog)	ELP3	XP_534563.3
Bos taurus (cattle)	ELP3	NP_001124234.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008300	XP_317165.4
Kluyveromyces lactis	KLLA0E23937g	XP_455030.1
Magnaporthe oryzae (rice blast fungus)	MGG_05481	XP_003710346.1
Neurospora crassa	NCU01229	XP_961595.1
Arabidopsis thaliana (thale cress)	ELO3	NP_568725.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both Elp3-induced acetylation and Hos2-induced deacetylation influence the nucleosome positions in the promoters of 24 genes
Title: Comparative studies of genome-wide maps of nucleosomes between deletion mutants of elp3 and hos2 genes of Saccharomyces cerevisiae.

We present our working model in which Elp3/Gcn5 and the APC work together to facilitate passage through mitosis and G(1). To progress into S, we propose that at least Gcn5 must then be targeted for degradation in an APC-dependent fashion.
Title: The Saccharomyces cerevisiae anaphase-promoting complex interacts with multiple histone-modifying enzymes to regulate cell cycle progression.

Results conclude that yeast and plant Elongator (elp3/ELO3) share tRNA modification roles and propose that this function might be conserved in Elongator from all eukaryotic kingdoms of life.
Title: Elongator function in tRNA wobble uridine modification is conserved between yeast and plants.

report an interaction between the Elongator histone acetyltransferase and the proliferating cell nuclear antigen. Cells lacking Elp3 and are sensitive to the DNA replication inhibitor hydroxyurea and the damaging agent methyl methanesulfonate.
Title: The elongator complex interacts with PCNA and modulates transcriptional silencing and sensitivity to DNA damage agents.

the Elp3 FeS cluster is essential for normal Elongator function in vivo primarily as a structural, rather than catalytic, domain.
Title: An iron-sulfur cluster domain in Elp3 important for the structural integrity of elongator.

The following ELP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ELP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04885	NM_001183900.1 Latest version!	Saccharomyces cerevisiae S288C Elongator subunit ELP3 (ELP3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ELP3 ( NM_001183900.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015239.1 Saccharomyces cerevisiae S288C Elongator subunit ELP3 (ELP3), partial mRNA.

Price & Availability↑

CloneID	OSi04885
Clone ID Related Accession (Same CDS sequence)	NM_001183900.1
Accession Version	NM_001183900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Elongator subunit ELP3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTCGTC ATGGAAAAGG CCCAAAAACT AACAAAAAAA AGCTAGCACC TGAAAAGGAA 
AGGTTTATAC AATGTTGTGC TGATATCACA TTAGAGTTAA CAGATTCTTT AACCTCGGGA 
ACAACAAGAG AAATTAATCT GAATGGTTTG ATTACTAAAT ATTCAAAGAA ATATAAACTA 
AAGCAACAAC CAAGGTTAAC CGATATCATT AATTCTATTC CAGACCAATA CAAAAAATAT 
TTATTACCCA AATTGAAGGC TAAGCCAGTA AGAACAGCAT CGGGTATTGC AGTGGTAGCC 
GTTATGTGTA AACCACATCG TTGTCCTCAC ATTGCATATA CAGGTAATAT TTGTGTTTAT 
TGTCCAGGTG GTCCAGATTC AGATTTTGAG TATTCTACAC AATCTTACAC AGGCTATGAA 
CCAACTTCAA TGCGTGCCAT CAGAGCTCGT TATGACCCTT ATGAACAAGC ACGTGGTAGG 
GTGGAACAAT TGAAACAGTT AGGTCACTCC ATCGATAAAG TCGAATATGT TCTGATGGGT 
GGTACATTTA TGTCATTACC AAAAGAATAT CGTGAAGATT TTATTGTGAA ATTGCATAAT 
GCACTTTCTG GGTTCAATGG TAATGATATT GATGAAGCTA TTCTTTATTC GCAACAAAGT 
TTAACGAAGT GTGTTGGTAT AACAATCGAA ACTAGGCCTG ATTATTGTAC GCAAACACAT 
TTGGACGATA TGTTAAAATA TGGCTGTACC AGATTAGAAA TTGGTGTTCA GTCCTTGTAC 
GAAGACGTTG CTCGTGATAC TAATAGAGGA CACACCGTTA GGTCTGTTTG TGAAACTTTT 
GCTGTGTCTA AAGATGCTGG TTATAAGGTT GTTTCTCACA TGATGCCAGA TTTACCAAAT 
GTTGGGATGG AAAGAGATAT TGAACAGTTC AAAGAGTATT TTGAAAATCC TGATTTTAGG 
ACTGATGGGT TGAAAATCTA TCCAACTTTA GTCATTAGGG GTACAGGTTT ATACGAACTT 
TGGAAAACGG GCAGGTATAA GTCTTATAGT GCCAACGCCT TAGTGGACTT AGTTGCTAGA 
ATCTTGGCCC TAGTGCCCCC ATGGACAAGA ATCTACCGTG TCCAAAGAGA CATTCCAATG 
CCCTTGGTTA CCTCAGGTGT TGACAATGGT AACTTGAGAG AATTGGCATT AGCTAGAATG 
AAGGACTTGG GTACAACTTG TAGGGATGTT CGTACTAGGG AAGTGGGTAT CCAAGAAGTG 
CATCATAAAG TTCAACCAGA TCAGGTCGAG CTTATCAGAA GGGATTACTA TGCAAATGGT 
GGTTGGGAAA CCTTTTTATC ATATGAAGAT CCAAAGAAAG ATATACTGAT TGGTTTGCTG 
AGATTGAGAA AGGCTTCAAA GAAATATACA TATAGAAAAG AATTCACCTC CCAGAGGACT 
TCTATTGTTA GAGAATTGCA TGTTTATGGT TCTGTGGTTC CACTTCATTC AAGAGATCCT 
CGTAAATTCC AGCATCAAGG GTTTGGTACT CTATTGATGG AAGAAGCGGA AAGAATCGCC 
AAGGAAGAGC ATGGTTCAGA GAAAATTTCT GTTATTTCTG GTGTTGGTGT AAGAAATTAC 
TATGGTAAAC TAGGATATGA ACTAGACGGT CCATACATGT CGAAAAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015239.1
CDS	1..1674
Translation	MARHGKGPKTNKKKLAPEKERFIQCCADITLELTDSLTSGTTREINLNGLITKYSKKYKLKQQPRLTDIINSIPDQYKKYLLPKLKAKPVRTASGIAVVAVMCKPHRCPHIAYTGNICVYCPGGPDSDFEYSTQSYTGYEPTSMRAIRARYDPYEQARGRVEQLKQLGHSIDKVEYVLMGGTFMSLPKEYREDFIVKLHNALSGFNGNDIDEAILYSQQSLTKCVGITIETRPDYCTQTHLDDMLKYGCTRLEIGVQSLYEDVARDTNRGHTVRSVCETFAVSKDAGYKVVSHMMPDLPNVGMERDIEQFKEYFENPDFRTDGLKIYPTLVIRGTGLYELWKTGRYKSYSANALVDLVARILALVPPWTRIYRVQRDIPMPLVTSGVDNGNLRELALARMKDLGTTCRDVRTREVGIQEVHHKVQPDQVELIRRDYYANGGWETFLSYEDPKKDILIGLLRLRKASKKYTYRKEFTSQRTSIVRELHVYGSVVPLHSRDPRKFQHQGFGTLLMEEAERIAKEEHGSEKISVISGVGVRNYYGKLGYELDGPYMSKRI

Target ORF information:

RefSeq Version	NM_001183900.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Elongator subunit ELP3 (ELP3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183900.1

ORF Insert Sequence:

ATGGCTCGTC ATGGAAAAGG CCCAAAAACT AACAAAAAAA AGCTAGCACC TGAAAAGGAA 
AGGTTTATAC AATGTTGTGC TGATATCACA TTAGAGTTAA CAGATTCTTT AACCTCGGGA 
ACAACAAGAG AAATTAATCT GAATGGTTTG ATTACTAAAT ATTCAAAGAA ATATAAACTA 
AAGCAACAAC CAAGGTTAAC CGATATCATT AATTCTATTC CAGACCAATA CAAAAAATAT 
TTATTACCCA AATTGAAGGC TAAGCCAGTA AGAACAGCAT CGGGTATTGC AGTGGTAGCC 
GTTATGTGTA AACCACATCG TTGTCCTCAC ATTGCATATA CAGGTAATAT TTGTGTTTAT 
TGTCCAGGTG GTCCAGATTC AGATTTTGAG TATTCTACAC AATCTTACAC AGGCTATGAA 
CCAACTTCAA TGCGTGCCAT CAGAGCTCGT TATGACCCTT ATGAACAAGC ACGTGGTAGG 
GTGGAACAAT TGAAACAGTT AGGTCACTCC ATCGATAAAG TCGAATATGT TCTGATGGGT 
GGTACATTTA TGTCATTACC AAAAGAATAT CGTGAAGATT TTATTGTGAA ATTGCATAAT 
GCACTTTCTG GGTTCAATGG TAATGATATT GATGAAGCTA TTCTTTATTC GCAACAAAGT 
TTAACGAAGT GTGTTGGTAT AACAATCGAA ACTAGGCCTG ATTATTGTAC GCAAACACAT 
TTGGACGATA TGTTAAAATA TGGCTGTACC AGATTAGAAA TTGGTGTTCA GTCCTTGTAC 
GAAGACGTTG CTCGTGATAC TAATAGAGGA CACACCGTTA GGTCTGTTTG TGAAACTTTT 
GCTGTGTCTA AAGATGCTGG TTATAAGGTT GTTTCTCACA TGATGCCAGA TTTACCAAAT 
GTTGGGATGG AAAGAGATAT TGAACAGTTC AAAGAGTATT TTGAAAATCC TGATTTTAGG 
ACTGATGGGT TGAAAATCTA TCCAACTTTA GTCATTAGGG GTACAGGTTT ATACGAACTT 
TGGAAAACGG GCAGGTATAA GTCTTATAGT GCCAACGCCT TAGTGGACTT AGTTGCTAGA 
ATCTTGGCCC TAGTGCCCCC ATGGACAAGA ATCTACCGTG TCCAAAGAGA CATTCCAATG 
CCCTTGGTTA CCTCAGGTGT TGACAATGGT AACTTGAGAG AATTGGCATT AGCTAGAATG 
AAGGACTTGG GTACAACTTG TAGGGATGTT CGTACTAGGG AAGTGGGTAT CCAAGAAGTG 
CATCATAAAG TTCAACCAGA TCAGGTCGAG CTTATCAGAA GGGATTACTA TGCAAATGGT 
GGTTGGGAAA CCTTTTTATC ATATGAAGAT CCAAAGAAAG ATATACTGAT TGGTTTGCTG 
AGATTGAGAA AGGCTTCAAA GAAATATACA TATAGAAAAG AATTCACCTC CCAGAGGACT 
TCTATTGTTA GAGAATTGCA TGTTTATGGT TCTGTGGTTC CACTTCATTC AAGAGATCCT 
CGTAAATTCC AGCATCAAGG GTTTGGTACT CTATTGATGG AAGAAGCGGA AAGAATCGCC 
AAGGAAGAGC ATGGTTCAGA GAAAATTTCT GTTATTTCTG GTGTTGGTGT AAGAAATTAC 
TATGGTAAAC TAGGATATGA ACTAGACGGT CCATACATGT CGAAAAGAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ELP3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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