RAD53 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following RAD53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04821 NM_001183967.1
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Saccharomyces cerevisiae S288C serine/threonine/tyrosine protein kinase RAD53 (RAD53), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi04821
    Clone ID Related Accession (Same CDS sequence) NM_001183967.1
    Accession Version NM_001183967.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product serine/threonine/tyrosine protein kinase RAD53
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAAATA TTACACAACC CACACAGCAA TCCACGCAGG CTACTCAAAG GTTTTTGATT 
    GAGAAGTTTT CTCAAGAACA GATCGGCGAA AACATTGTGT GCAGGGTCAT TTGTACCACG
    GGTCAAATTC CCATCCGAGA TTTGTCAGCT GATATTTCAC AAGTGCTTAA GGAAAAACGA
    TCCATAAAGA AAGTTTGGAC ATTTGGTAGA AACCCAGCCT GTGACTATCA TTTAGGAAAC
    ATTTCAAGAC TGTCAAATAA GCATTTCCAA ATACTACTAG GAGAAGACGG TAACCTTTTA
    TTGAATGACA TTTCCACTAA TGGGACCTGG TTAAATGGGC AAAAAGTCGA GAAGAACAGC
    AATCAGTTAC TGTCTCAAGG TGATGAAATA ACCGTTGGTG TAGGCGTGGA ATCAGATATT
    TTATCTCTGG TCATTTTCAT AAACGACAAA TTTAAGCAGT GCCTCGAGCA GAACAAAGTT
    GATCGCATAA GATCTAACCT GAAAAATACC TCTAAAATAG CTTCTCCTGG TCTTACATCA
    TCTACTGCAT CATCAATGGT GGCCAACAAG ACTGGTATTT TTAAGGATTT TTCGATTATT
    GACGAAGTGG TGGGCCAGGG TGCATTTGCC ACAGTAAAGA AAGCCATTGA AAGAACTACT
    GGGAAAACAT TCGCGGTGAA GATTATAAGT AAACGCAAAG TAATAGGCAA TATGGATGGT
    GTGACAAGAG AGTTAGAAGT ATTGCAAAAG CTCAATCATC CAAGGATAGT ACGATTGAAA
    GGATTTTATG AAGATACTGA GAGTTATTAT ATGGTGATGG AGTTCGTTTC TGGTGGTGAC
    TTAATGGATT TTGTTGCTGC TCATGGTGCG GTTGGAGAAG ATGCTGGGAG GGAGATATCC
    AGGCAGATAC TCACAGCAAT AAAATACATT CACTCTATGG GCATCAGCCA TCGTGACCTA
    AAGCCCGATA ATATTCTTAT TGAACAAGAC GATCCTGTAT TGGTAAAGAT AACCGACTTT
    GGTCTGGCAA AAGTACAAGG AAATGGGTCT TTTATGAAAA CCTTCTGTGG CACTTTGGCA
    TATGTGGCAC CTGAAGTCAT CAGAGGTAAA GATACATCCG TATCTCCTGA TGAATACGAA
    GAAAGGAATG AGTACTCTTC GTTAGTGGAT ATGTGGTCAA TGGGATGTCT TGTGTATGTT
    ATCCTAACGG GCCACTTACC TTTTAGTGGT AGCACACAGG ACCAATTATA TAAACAGATT
    GGAAGAGGCT CATATCATGA AGGGCCCCTC AAAGATTTCC GGATATCTGA AGAAGCAAGA
    GATTTCATAG ATTCATTGTT ACAGGTGGAT CCAAATAATA GGTCGACAGC TGCAAAAGCC
    TTGAATCATC CCTGGATCAA GATGAGTCCA TTGGGCTCAC AATCATATGG TGATTTTTCA
    CAAATATCCT TATCACAATC GTTGTCGCAG CAGAAATTAT TAGAAAATAT GGACGATGCT
    CAATACGAAT TTGTCAAAGC GCAAAGGAAA TTACAAATGG AGCAACAACT TCAAGAACAG
    GATCAGGAAG ACCAAGATGG AAAAATTCAA GGATTTAAAA TACCCGCACA CGCCCCTATT
    CGATATACAC AGCCCAAAAG CATTGAAGCA GAAACTAGAG AACAAAAACT TTTACATTCC
    AATAATACTG AGAATGTCAA GAGCTCAAAG AAAAAGGGTA ATGGTAGGTT TTTAACTTTA
    AAACCATTGC CTGACAGCAT TATTCAAGAA AGCCTGGAGA TTCAGCAAGG TGTGAATCCA
    TTTTTCATTG GTAGATCCGA GGATTGCAAT TGTAAAATTG AAGACAATAG GTTGTCTCGA
    GTTCATTGCT TCATTTTCAA AAAGAGGCAT GCTGTAGGCA AAAGCATGTA TGAATCTCCG
    GCACAAGGTT TAGATGATAT TTGGTATTGC CACACCGGAA CTAACGTGAG CTATTTAAAT
    AATAACCGCA TGATACAGGG TACGAAATTC CTTTTACAAG ACGGAGATGA AATCAAGATC
    ATTTGGGATA AAAACAATAA ATTTGTCATT GGCTTTAAAG TGGAAATTAA CGATACTACA
    GGTCTGTTTA ACGAGGGATT AGGTATGTTA CAAGAACAAA GAGTAGTACT TAAGCAAACA
    GCCGAAGAAA AAGATTTGGT GAAAAAGTTA ACCCAGATGA TGGCAGCTCA ACGTGCAAAT
    CAACCCTCGG CTTCTTCTTC ATCAATGTCG GCTAAGAAGC CGCCAGTTAG CGATACAAAT
    AATAACGGCA ATAATTCGGT ACTAAACGAC TTGGTAGAGT CACCGATTAA TGCGAATACG
    GGGAACATTT TGAAGAGAAT ACATTCGGTA AGTTTATCGC AATCACAAAT TGATCCTAGT
    AAGAAGGTTA AAAGGGCAAA ATTGGACCAA ACCTCAAAAG GCCCCGAGAA TTTGCAATTT
    TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_015172.1
    CDS1..2466
    Translation

    Target ORF information:

    RefSeq Version NM_001183967.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C serine/threonine/tyrosine protein kinase RAD53 (RAD53), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001183967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAAAATA TTACACAACC CACACAGCAA TCCACGCAGG CTACTCAAAG GTTTTTGATT 
    GAGAAGTTTT CTCAAGAACA GATCGGCGAA AACATTGTGT GCAGGGTCAT TTGTACCACG
    GGTCAAATTC CCATCCGAGA TTTGTCAGCT GATATTTCAC AAGTGCTTAA GGAAAAACGA
    TCCATAAAGA AAGTTTGGAC ATTTGGTAGA AACCCAGCCT GTGACTATCA TTTAGGAAAC
    ATTTCAAGAC TGTCAAATAA GCATTTCCAA ATACTACTAG GAGAAGACGG TAACCTTTTA
    TTGAATGACA TTTCCACTAA TGGGACCTGG TTAAATGGGC AAAAAGTCGA GAAGAACAGC
    AATCAGTTAC TGTCTCAAGG TGATGAAATA ACCGTTGGTG TAGGCGTGGA ATCAGATATT
    TTATCTCTGG TCATTTTCAT AAACGACAAA TTTAAGCAGT GCCTCGAGCA GAACAAAGTT
    GATCGCATAA GATCTAACCT GAAAAATACC TCTAAAATAG CTTCTCCTGG TCTTACATCA
    TCTACTGCAT CATCAATGGT GGCCAACAAG ACTGGTATTT TTAAGGATTT TTCGATTATT
    GACGAAGTGG TGGGCCAGGG TGCATTTGCC ACAGTAAAGA AAGCCATTGA AAGAACTACT
    GGGAAAACAT TCGCGGTGAA GATTATAAGT AAACGCAAAG TAATAGGCAA TATGGATGGT
    GTGACAAGAG AGTTAGAAGT ATTGCAAAAG CTCAATCATC CAAGGATAGT ACGATTGAAA
    GGATTTTATG AAGATACTGA GAGTTATTAT ATGGTGATGG AGTTCGTTTC TGGTGGTGAC
    TTAATGGATT TTGTTGCTGC TCATGGTGCG GTTGGAGAAG ATGCTGGGAG GGAGATATCC
    AGGCAGATAC TCACAGCAAT AAAATACATT CACTCTATGG GCATCAGCCA TCGTGACCTA
    AAGCCCGATA ATATTCTTAT TGAACAAGAC GATCCTGTAT TGGTAAAGAT AACCGACTTT
    GGTCTGGCAA AAGTACAAGG AAATGGGTCT TTTATGAAAA CCTTCTGTGG CACTTTGGCA
    TATGTGGCAC CTGAAGTCAT CAGAGGTAAA GATACATCCG TATCTCCTGA TGAATACGAA
    GAAAGGAATG AGTACTCTTC GTTAGTGGAT ATGTGGTCAA TGGGATGTCT TGTGTATGTT
    ATCCTAACGG GCCACTTACC TTTTAGTGGT AGCACACAGG ACCAATTATA TAAACAGATT
    GGAAGAGGCT CATATCATGA AGGGCCCCTC AAAGATTTCC GGATATCTGA AGAAGCAAGA
    GATTTCATAG ATTCATTGTT ACAGGTGGAT CCAAATAATA GGTCGACAGC TGCAAAAGCC
    TTGAATCATC CCTGGATCAA GATGAGTCCA TTGGGCTCAC AATCATATGG TGATTTTTCA
    CAAATATCCT TATCACAATC GTTGTCGCAG CAGAAATTAT TAGAAAATAT GGACGATGCT
    CAATACGAAT TTGTCAAAGC GCAAAGGAAA TTACAAATGG AGCAACAACT TCAAGAACAG
    GATCAGGAAG ACCAAGATGG AAAAATTCAA GGATTTAAAA TACCCGCACA CGCCCCTATT
    CGATATACAC AGCCCAAAAG CATTGAAGCA GAAACTAGAG AACAAAAACT TTTACATTCC
    AATAATACTG AGAATGTCAA GAGCTCAAAG AAAAAGGGTA ATGGTAGGTT TTTAACTTTA
    AAACCATTGC CTGACAGCAT TATTCAAGAA AGCCTGGAGA TTCAGCAAGG TGTGAATCCA
    TTTTTCATTG GTAGATCCGA GGATTGCAAT TGTAAAATTG AAGACAATAG GTTGTCTCGA
    GTTCATTGCT TCATTTTCAA AAAGAGGCAT GCTGTAGGCA AAAGCATGTA TGAATCTCCG
    GCACAAGGTT TAGATGATAT TTGGTATTGC CACACCGGAA CTAACGTGAG CTATTTAAAT
    AATAACCGCA TGATACAGGG TACGAAATTC CTTTTACAAG ACGGAGATGA AATCAAGATC
    ATTTGGGATA AAAACAATAA ATTTGTCATT GGCTTTAAAG TGGAAATTAA CGATACTACA
    GGTCTGTTTA ACGAGGGATT AGGTATGTTA CAAGAACAAA GAGTAGTACT TAAGCAAACA
    GCCGAAGAAA AAGATTTGGT GAAAAAGTTA ACCCAGATGA TGGCAGCTCA ACGTGCAAAT
    CAACCCTCGG CTTCTTCTTC ATCAATGTCG GCTAAGAAGC CGCCAGTTAG CGATACAAAT
    AATAACGGCA ATAATTCGGT ACTAAACGAC TTGGTAGAGT CACCGATTAA TGCGAATACG
    GGGAACATTT TGAAGAGAAT ACATTCGGTA AGTTTATCGC AATCACAAAT TGATCCTAGT
    AAGAAGGTTA AAAGGGCAAA ATTGGACCAA ACCTCAAAAG GCCCCGAGAA TTTGCAATTT
    TCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
    Nature387(6632 Suppl)103-5(1997 May)
    Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG