REV3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	REV3
Entrez Gene ID	855936
Full Name	Rev3p
Synonyms	PSO1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183981.1	NP_015158.1	DNA-directed DNA polymerase

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	REV3	NP_015158.1
Kluyveromyces lactis	KLLA0C09911g	XP_452640.1
Magnaporthe oryzae (rice blast fungus)	MGG_02986	XP_003720713.1
Neurospora crassa	NCU01951	XP_964235.1
Schizosaccharomyces pombe (fission yeast)	rev3	NP_594068.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Profiles of base-substitutions created by Polzeta & Poleta of Saccharomyces cerevisiae were analyzed. Poleta created deletions at the lesion in more than 50% of the TLS products, but the formation of the deletions was suppressed by the presence of Polzeta. Polzeta & Poleta cooperatively facilitated the TLS reaction over an abasic site in vitro, suggesting that these two polymerases can cooperate in efficient and high f...
Title: NGS-based analysis of base-substitution signatures created by yeast DNA polymerase eta and zeta on undamaged and abasic DNA templates in vitro.

This study also uncovered an important role for Rev3 and Rev7 (but not for Pol32) subunits of polymerase zeta in the survival of cells undergoing telomere losses and forming palindromes
Title: Telomere Dysfunction Triggers Palindrome Formation Independently of Double-Strand Break Repair Mechanisms.

A novel mutation affecting the catalytic subunit Rev3 and the role of Rev3 in the Pol32 regulation
Title: A novel variant of DNA polymerase ?, Rev3?C, highlights differential regulation of Pol32 as a subunit of polymerase ? versus ? in Saccharomyces cerevisiae.

Pol zeta, the enzyme responsible for the majority of damage-induced mutagenesis in eukaryotes, the two-subunit (Rev3-Rev7) form of yeast Pol zeta used in this study suggest that ribonucleotide incorporation into DNA during Pol zeta-mediated mutagenesis in vivo may be rare.
Title: Ribonucleotide incorporation by yeast DNA polymerase ?.

study found both Pol zeta and Pol eta are necessary for efficient gap repair and that each functions independently of the other; these polymerases may be involved either in initiation of DNA synthesis from the invading end or in a gap-filling process that is required to complete recombination
Title: Roles of exonucleases and translesion synthesis DNA polymerases during mitotic gap repair in yeast.

The following REV3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the REV3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04807	NM_001183981.1 Latest version!	Saccharomyces cerevisiae S288C DNA-directed DNA polymerase (REV3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

REV3 ( NM_001183981.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015158.1 Saccharomyces cerevisiae S288C DNA-directed DNA polymerase (REV3), partial mRNA.

Price & Availability↑

CloneID	OSi04807
Clone ID Related Accession (Same CDS sequence)	NM_001183981.1
Accession Version	NM_001183981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-directed DNA polymerase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGAGGG AGTCGAACGA CACAATACAG AGCGATACGG TTAGATCATC CTCTAAATCA 
GACTATTTTA GAATCCAGCT AAATAATCAA GACTACTATA TGTCAAAACC CACCTTTTTG 
GACCCATCGC ACGGTGAAAG CCTGCCCTTA AACCAGTTTT CACAGGTCCC AAACATCAGG 
GTGTTTGGTG CTTTACCCAC AGGGCATCAA GTATTGTGCC ACGTCCATGG CATTCTACCT 
TACATGTTCA TCAAGTATGA TGGACAAATA ACTGACACGA GTACATTGAG ACACCAAAGA 
TGTGCCCAAG TTCATAAAAC GCTGGAAGTA AAAATTAGGG CATCCTTTAA AAGGAAAAAA 
GATGATAAAC ACGATTTAGC CGGCGACAAA CTTGGAAATC TCAATTTTGT CGCTGATGTC 
TCTGTTGTAA AGGGTATACC ATTTTATGGT TACCACGTTG GCTGGAATCT GTTTTACAAA 
ATTTCTTTGC TTAATCCTTC TTGTCTAAGT AGGATTTCGG AATTGATTAG AGATGGCAAG 
ATTTTCGGAA AGAAATTCGA AATTTATGAA TCACATATTC CTTACTTGCT ACAATGGACC 
GCAGATTTTA ACTTATTTGG ATGTTCATGG ATAAACGTGG ATAGGTGTTA TTTTCGCTCT 
CCTGTTTTAA ATAGTATACT GGACATAGAT AAGCTGACAA TTAATGATGA TCTTCAGTTG 
TTACTAGATC GATTTTGTGA CTTCAAGTGT AACGTACTCA GTAGAAGGGA TTTTCCACGG 
GTTGGAAATG GATTGATTGA GATAGATATA CTACCGCAAT TCATCAAAAA TAGAGAAAAG 
TTACAGCATA GAGATATACA TCACGACTTT TTGGAAAAAC TGGGAGATAT TTCTGACATA 
CCTGTCAAAC CATATGTATC CTCTGCTAGG GACATGATAA ATGAACTTAC CATGCAACGA 
GAGGAGTTGT CCTTGAAGGA ATATAAAGAA CCACCAGAAA CAAAGCGCCA TGTGAGTGGT 
CATCAATGGC AATCTTCTGG AGAGTTCGAA GCGTTTTATA AGAAAGCACA ACATAAAACA 
AGTACTTTTG ATGGTCAAAT ACCAAATTTT GAGAACTTTA TAGATAAGAA TCAAAAATTC 
TCAGCCATAA ATACGCCCTA TGAAGCATTA CCGCAATTGT GGCCAAGGCT TCCACAGATT 
GAAATAAACA ATAATAGTAT GCAAGATAAA AAGAACGACG ACCAAGTTAA TGCATCGTTC 
ACCGAATACG AGATATGTGG TGTTGATAAT GAGAACGAAG GTGTAAAAGG AAGCAATATA 
AAGTCTCGGT CTTATTCATG GTTACCGGAA AGCATTGCGT CTCCGAAAGA TTCTACTATA 
CTATTAGATC ATCAAACAAA ATATCACAAT ACGATAAATT TTTCAATGGA TTGTGCTATG 
ACGCAAAATA TGGCAAGTAA ACGAAAACTT AGATCATCTG TTTCTGCTAA TAAAACATCG 
TTGCTGTCTC GGAAAAGAAA GAAGGTTATG GCTGCGGGAT TACGCTATGG AAAAAGAGCC 
TTTGTTTATG GTGAGCCTCC TTTCGGTTAT CAAGATATTC TGAATAAATT GGAAGACGAG 
GGATTCCCAA AAATAGACTA TAAGGACCCT TTCTTTTCGA ATCCAGTTGA TCTAGAAAAT 
AAACCTTACG CGTACGCAGG TAAGAGGTTT GAAATAAGCT CGACACATGT ATCAACGAGA 
ATACCCGTCC AATTTGGGGG AGAGACCGTA TCAGTTTATA ACAAGCCAAC TTTCGACATG 
TTTTCCTCCT GGAAATATGC GTTAAAACCA CCTACGTATG ATGCTGTTCA AAAATGGTAC 
AATAAAGTAC CCTCGATGGG AAACAAAAAA ACTGAGTCTC AGATAAGCAT GCACACCCCT 
CATAGTAAGT TTTTATACAA ATTTGCTAGT GACGTTTCTG GAAAACAAAA AAGAAAAAAA 
AGTAGCGTTC ACGATTCTCT TACTCATCTG ACACTTGAGA TTCATGCAAA CACGAGAAGT 
GATAAAATTC CAGACCCTGC AATAGATGAA GTTTCCATGA TTATATGGTG TCTTGAAGAA 
GAAACTTTCC CTTTAGATTT GGATATCGCT TACGAAGGGA TTATGATAGT CCACAAAGCT 
TCTGAGGATA GTACATTTCC TACTAAAATT CAACATTGTA TTAACGAAAT TCCCGTGATG 
TTCTATGAAA GTGAATTTGA AATGTTTGAG GCTCTAACTG ATTTGGTATT ACTTCTTGAT 
CCTGATATTC TTTCCGGTTT CGAAATACAT AATTTTTCGT GGGGTTATAT AATTGAAAGG 
TGTCAAAAAA TACATCAATT TGACATTGTC AGGGAACTTG CCAGAGTCAA ATGCCAAATT 
AAGACGAAGT TGTCAGATAC TTGGGGATAT GCCCATTCCT CAGGAATTAT GATTACCGGA 
AGACATATGA TTAATATATG GAGAGCGTTG AGGTCCGACG TAAATTTAAC GCAATATACT 
ATTGAAAGTG CTGCGTTCAA TATCCTCCAT AAACGATTAC CTCACTTTTC ATTTGAGTCA 
TTAACTAATA TGTGGAACGC TAAAAAGAGC ACAACCGAAT TAAAGACTGT GTTAAATTAT 
TGGTTATCAA GAGCTCAGAT AAATATACAA CTATTAAGAA AGCAGGATTA CATTGCTCGA 
AATATCGAAC AGGCAAGATT GATAGGAATA GATTTCCATT CTGTATATTA CAGAGGATCA 
CAATTTAAAG TTGAGTCTTT TTTAATCCGA ATATGCAAGT CCGAAAGTTT TATCCTTCTT 
TCTCCAGGCA AAAAGGATGT TCGTAAGCAA AAAGCACTTG AATGTGTGCC TTTGGTTATG 
GAGCCAGAAT CTGCTTTCTA CAAAAGTCCT TTAATTGTGC TGGATTTCCA ATCATTGTAT 
CCATCCATTA TGATTGGATA CAACTATTGC TATTCGACTA TGATAGGAAG AGTGCGAGAA 
ATAAACTTAA CGGAAAATAA CCTTGGAGTA TCTAAGTTTT CATTACCAAG AAACATTTTA 
GCTTTATTAA AAAATGATGT AACTATCGCT CCAAATGGTG TTGTTTATGC CAAGACCTCT 
GTTAGAAAAT CAACGTTATC CAAAATGTTA ACAGATATCC TTGATGTCAG AGTGATGATA 
AAGAAAACAA TGAACGAAAT AGGTGATGAC AACACTACCC TAAAAAGGCT TTTGAATAAC 
AAACAGTTAG CACTAAAATT ATTGGCGAAT GTCACCTACG GTTATACATC AGCTTCATTT 
TCTGGACGAA TGCCATGCTC TGATTTAGCT GATAGCATTG TACAAACAGG CAGAGAAACA 
TTGGAGAAAG CAATAGATAT TATTGAGAAA GATGAAACTT GGAACGCCAA AGTTGTCTAT 
GGAGACACAG ATAGTTTATT TGTATATCTA CCTGGAAAAA CTGCTATTGA GGCTTTTTCT 
ATAGGACATG CTATGGCAGA AAGAGTTACT CAAAACAATC CAAAACCAAT CTTTTTGAAG 
TTTGAAAAAG TATACCATCC CTCCATATTA ATTAGCAAAA AAAGGTACGT AGGATTTTCC 
TATGAAAGTC CTTCGCAGAC CCTTCCTATT TTTGATGCTA AGGGTATTGA AACTGTTAGA 
AGGGATGGTA TCCCAGCCCA GCAGAAGATT ATTGAAAAAT GTATTCGATT ACTTTTTCAA 
ACCAAAGACC TGTCAAAAAT AAAAAAGTAT CTTCAAAATG AATTTTTTAA GATTCAAATA 
GGAAAGGTAT CTGCCCAAGA TTTTTGTTTT GCAAAAGAAG TTAAATTAGG AGCGTATAAA 
AGCGAAAAGA CAGCCCCTGC AGGTGCCGTG GTTGTAAAAA GAAGAATAAA TGAAGACCAT 
AGAGCAGAAC CCCAATACAA GGAGCGTATA CCTTACCTTG TTGTTAAAGG AAAGCAAGGA 
CAACTGCTTC GGGAAAGATG CGTATCACCA GAAGAGTTCT TAGAAGGTGA GAATTTAGAG 
TTAGATTCGG AGTATTATAT AAACAAAATT CTGATACCTC CTCTTGATAG ATTGTTCAAT 
TTGATTGGTA TAAATGTTGG CAACTGGGCT CAGGAAATAG TAAAATCCAA AAGGGCGAGC 
ACAACTACTA CAAAAGTGGA AAACATAACA AGAGTAGGAA CTTCTGCAAC ATGTTGTAAT 
TGTGGTGAAG AATTGACTAA AATATGTTCA CTTCAGTTAT GTGATGACTG TTTAGAGAAA 
AGAAGCACCA CAACCTTATC ATTTCTCATA AAGAAATTAA AGAGACAAAA AGAATACCAA 
ACACTAAAGA CCGTGTGCAG GACGTGCAGT TATCGTTACA CTTCCGATGC AGGCATCGAA 
AATGACCATA TAGCTAGTAA ATGCAATTCA TATGACTGTC CAGTATTTTA CTCTCGTGTC 
AAAGCAGAAA GATACTTGAG AGATAATCAA TCTGTTCAAA GGGAAGAAGC ATTAATATCT 
CTAAATGATT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015158.1
CDS	1..4515
Translation	MSRESNDTIQSDTVRSSSKSDYFRIQLNNQDYYMSKPTFLDPSHGESLPLNQFSQVPNIRVFGALPTGHQVLCHVHGILPYMFIKYDGQITDTSTLRHQRCAQVHKTLEVKIRASFKRKKDDKHDLAGDKLGNLNFVADVSVVKGIPFYGYHVGWNLFYKISLLNPSCLSRISELIRDGKIFGKKFEIYESHIPYLLQWTADFNLFGCSWINVDRCYFRSPVLNSILDIDKLTINDDLQLLLDRFCDFKCNVLSRRDFPRVGNGLIEIDILPQFIKNREKLQHRDIHHDFLEKLGDISDIPVKPYVSSARDMINELTMQREELSLKEYKEPPETKRHVSGHQWQSSGEFEAFYKKAQHKTSTFDGQIPNFENFIDKNQKFSAINTPYEALPQLWPRLPQIEINNNSMQDKKNDDQVNASFTEYEICGVDNENEGVKGSNIKSRSYSWLPESIASPKDSTILLDHQTKYHNTINFSMDCAMTQNMASKRKLRSSVSANKTSLLSRKRKKVMAAGLRYGKRAFVYGEPPFGYQDILNKLEDEGFPKIDYKDPFFSNPVDLENKPYAYAGKRFEISSTHVSTRIPVQFGGETVSVYNKPTFDMFSSWKYALKPPTYDAVQKWYNKVPSMGNKKTESQISMHTPHSKFLYKFASDVSGKQKRKKSSVHDSLTHLTLEIHANTRSDKIPDPAIDEVSMIIWCLEEETFPLDLDIAYEGIMIVHKASEDSTFPTKIQHCINEIPVMFYESEFEMFEALTDLVLLLDPDILSGFEIHNFSWGYIIERCQKIHQFDIVRELARVKCQIKTKLSDTWGYAHSSGIMITGRHMINIWRALRSDVNLTQYTIESAAFNILHKRLPHFSFESLTNMWNAKKSTTELKTVLNYWLSRAQINIQLLRKQDYIARNIEQARLIGIDFHSVYYRGSQFKVESFLIRICKSESFILLSPGKKDVRKQKALECVPLVMEPESAFYKSPLIVLDFQSLYPSIMIGYNYCYSTMIGRVREINLTENNLGVSKFSLPRNILALLKNDVTIAPNGVVYAKTSVRKSTLSKMLTDILDVRVMIKKTMNEIGDDNTTLKRLLNNKQLALKLLANVTYGYTSASFSGRMPCSDLADSIVQTGRETLEKAIDIIEKDETWNAKVVYGDTDSLFVYLPGKTAIEAFSIGHAMAERVTQNNPKPIFLKFEKVYHPSILISKKRYVGFSYESPSQTLPIFDAKGIETVRRDGIPAQQKIIEKCIRLLFQTKDLSKIKKYLQNEFFKIQIGKVSAQDFCFAKEVKLGAYKSEKTAPAGAVVVKRRINEDHRAEPQYKERIPYLVVKGKQGQLLRERCVSPEEFLEGENLELDSEYYINKILIPPLDRLFNLIGINVGNWAQEIVKSKRASTTTTKVENITRVGTSATCCNCGEELTKICSLQLCDDCLEKRSTTTLSFLIKKLKRQKEYQTLKTVCRTCSYRYTSDAGIENDHIASKCNSYDCPVFYSRVKAERYLRDNQSVQREEALISLNDW

Target ORF information:

RefSeq Version	NM_001183981.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-directed DNA polymerase (REV3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183981.1

ORF Insert Sequence:

ATGTCGAGGG AGTCGAACGA CACAATACAG AGCGATACGG TTAGATCATC CTCTAAATCA 
GACTATTTTA GAATCCAGCT AAATAATCAA GACTACTATA TGTCAAAACC CACCTTTTTG 
GACCCATCGC ACGGTGAAAG CCTGCCCTTA AACCAGTTTT CACAGGTCCC AAACATCAGG 
GTGTTTGGTG CTTTACCCAC AGGGCATCAA GTATTGTGCC ACGTCCATGG CATTCTACCT 
TACATGTTCA TCAAGTATGA TGGACAAATA ACTGACACGA GTACATTGAG ACACCAAAGA 
TGTGCCCAAG TTCATAAAAC GCTGGAAGTA AAAATTAGGG CATCCTTTAA AAGGAAAAAA 
GATGATAAAC ACGATTTAGC CGGCGACAAA CTTGGAAATC TCAATTTTGT CGCTGATGTC 
TCTGTTGTAA AGGGTATACC ATTTTATGGT TACCACGTTG GCTGGAATCT GTTTTACAAA 
ATTTCTTTGC TTAATCCTTC TTGTCTAAGT AGGATTTCGG AATTGATTAG AGATGGCAAG 
ATTTTCGGAA AGAAATTCGA AATTTATGAA TCACATATTC CTTACTTGCT ACAATGGACC 
GCAGATTTTA ACTTATTTGG ATGTTCATGG ATAAACGTGG ATAGGTGTTA TTTTCGCTCT 
CCTGTTTTAA ATAGTATACT GGACATAGAT AAGCTGACAA TTAATGATGA TCTTCAGTTG 
TTACTAGATC GATTTTGTGA CTTCAAGTGT AACGTACTCA GTAGAAGGGA TTTTCCACGG 
GTTGGAAATG GATTGATTGA GATAGATATA CTACCGCAAT TCATCAAAAA TAGAGAAAAG 
TTACAGCATA GAGATATACA TCACGACTTT TTGGAAAAAC TGGGAGATAT TTCTGACATA 
CCTGTCAAAC CATATGTATC CTCTGCTAGG GACATGATAA ATGAACTTAC CATGCAACGA 
GAGGAGTTGT CCTTGAAGGA ATATAAAGAA CCACCAGAAA CAAAGCGCCA TGTGAGTGGT 
CATCAATGGC AATCTTCTGG AGAGTTCGAA GCGTTTTATA AGAAAGCACA ACATAAAACA 
AGTACTTTTG ATGGTCAAAT ACCAAATTTT GAGAACTTTA TAGATAAGAA TCAAAAATTC 
TCAGCCATAA ATACGCCCTA TGAAGCATTA CCGCAATTGT GGCCAAGGCT TCCACAGATT 
GAAATAAACA ATAATAGTAT GCAAGATAAA AAGAACGACG ACCAAGTTAA TGCATCGTTC 
ACCGAATACG AGATATGTGG TGTTGATAAT GAGAACGAAG GTGTAAAAGG AAGCAATATA 
AAGTCTCGGT CTTATTCATG GTTACCGGAA AGCATTGCGT CTCCGAAAGA TTCTACTATA 
CTATTAGATC ATCAAACAAA ATATCACAAT ACGATAAATT TTTCAATGGA TTGTGCTATG 
ACGCAAAATA TGGCAAGTAA ACGAAAACTT AGATCATCTG TTTCTGCTAA TAAAACATCG 
TTGCTGTCTC GGAAAAGAAA GAAGGTTATG GCTGCGGGAT TACGCTATGG AAAAAGAGCC 
TTTGTTTATG GTGAGCCTCC TTTCGGTTAT CAAGATATTC TGAATAAATT GGAAGACGAG 
GGATTCCCAA AAATAGACTA TAAGGACCCT TTCTTTTCGA ATCCAGTTGA TCTAGAAAAT 
AAACCTTACG CGTACGCAGG TAAGAGGTTT GAAATAAGCT CGACACATGT ATCAACGAGA 
ATACCCGTCC AATTTGGGGG AGAGACCGTA TCAGTTTATA ACAAGCCAAC TTTCGACATG 
TTTTCCTCCT GGAAATATGC GTTAAAACCA CCTACGTATG ATGCTGTTCA AAAATGGTAC 
AATAAAGTAC CCTCGATGGG AAACAAAAAA ACTGAGTCTC AGATAAGCAT GCACACCCCT 
CATAGTAAGT TTTTATACAA ATTTGCTAGT GACGTTTCTG GAAAACAAAA AAGAAAAAAA 
AGTAGCGTTC ACGATTCTCT TACTCATCTG ACACTTGAGA TTCATGCAAA CACGAGAAGT 
GATAAAATTC CAGACCCTGC AATAGATGAA GTTTCCATGA TTATATGGTG TCTTGAAGAA 
GAAACTTTCC CTTTAGATTT GGATATCGCT TACGAAGGGA TTATGATAGT CCACAAAGCT 
TCTGAGGATA GTACATTTCC TACTAAAATT CAACATTGTA TTAACGAAAT TCCCGTGATG 
TTCTATGAAA GTGAATTTGA AATGTTTGAG GCTCTAACTG ATTTGGTATT ACTTCTTGAT 
CCTGATATTC TTTCCGGTTT CGAAATACAT AATTTTTCGT GGGGTTATAT AATTGAAAGG 
TGTCAAAAAA TACATCAATT TGACATTGTC AGGGAACTTG CCAGAGTCAA ATGCCAAATT 
AAGACGAAGT TGTCAGATAC TTGGGGATAT GCCCATTCCT CAGGAATTAT GATTACCGGA 
AGACATATGA TTAATATATG GAGAGCGTTG AGGTCCGACG TAAATTTAAC GCAATATACT 
ATTGAAAGTG CTGCGTTCAA TATCCTCCAT AAACGATTAC CTCACTTTTC ATTTGAGTCA 
TTAACTAATA TGTGGAACGC TAAAAAGAGC ACAACCGAAT TAAAGACTGT GTTAAATTAT 
TGGTTATCAA GAGCTCAGAT AAATATACAA CTATTAAGAA AGCAGGATTA CATTGCTCGA 
AATATCGAAC AGGCAAGATT GATAGGAATA GATTTCCATT CTGTATATTA CAGAGGATCA 
CAATTTAAAG TTGAGTCTTT TTTAATCCGA ATATGCAAGT CCGAAAGTTT TATCCTTCTT 
TCTCCAGGCA AAAAGGATGT TCGTAAGCAA AAAGCACTTG AATGTGTGCC TTTGGTTATG 
GAGCCAGAAT CTGCTTTCTA CAAAAGTCCT TTAATTGTGC TGGATTTCCA ATCATTGTAT 
CCATCCATTA TGATTGGATA CAACTATTGC TATTCGACTA TGATAGGAAG AGTGCGAGAA 
ATAAACTTAA CGGAAAATAA CCTTGGAGTA TCTAAGTTTT CATTACCAAG AAACATTTTA 
GCTTTATTAA AAAATGATGT AACTATCGCT CCAAATGGTG TTGTTTATGC CAAGACCTCT 
GTTAGAAAAT CAACGTTATC CAAAATGTTA ACAGATATCC TTGATGTCAG AGTGATGATA 
AAGAAAACAA TGAACGAAAT AGGTGATGAC AACACTACCC TAAAAAGGCT TTTGAATAAC 
AAACAGTTAG CACTAAAATT ATTGGCGAAT GTCACCTACG GTTATACATC AGCTTCATTT 
TCTGGACGAA TGCCATGCTC TGATTTAGCT GATAGCATTG TACAAACAGG CAGAGAAACA 
TTGGAGAAAG CAATAGATAT TATTGAGAAA GATGAAACTT GGAACGCCAA AGTTGTCTAT 
GGAGACACAG ATAGTTTATT TGTATATCTA CCTGGAAAAA CTGCTATTGA GGCTTTTTCT 
ATAGGACATG CTATGGCAGA AAGAGTTACT CAAAACAATC CAAAACCAAT CTTTTTGAAG 
TTTGAAAAAG TATACCATCC CTCCATATTA ATTAGCAAAA AAAGGTACGT AGGATTTTCC 
TATGAAAGTC CTTCGCAGAC CCTTCCTATT TTTGATGCTA AGGGTATTGA AACTGTTAGA 
AGGGATGGTA TCCCAGCCCA GCAGAAGATT ATTGAAAAAT GTATTCGATT ACTTTTTCAA 
ACCAAAGACC TGTCAAAAAT AAAAAAGTAT CTTCAAAATG AATTTTTTAA GATTCAAATA 
GGAAAGGTAT CTGCCCAAGA TTTTTGTTTT GCAAAAGAAG TTAAATTAGG AGCGTATAAA 
AGCGAAAAGA CAGCCCCTGC AGGTGCCGTG GTTGTAAAAA GAAGAATAAA TGAAGACCAT 
AGAGCAGAAC CCCAATACAA GGAGCGTATA CCTTACCTTG TTGTTAAAGG AAAGCAAGGA 
CAACTGCTTC GGGAAAGATG CGTATCACCA GAAGAGTTCT TAGAAGGTGA GAATTTAGAG 
TTAGATTCGG AGTATTATAT AAACAAAATT CTGATACCTC CTCTTGATAG ATTGTTCAAT 
TTGATTGGTA TAAATGTTGG CAACTGGGCT CAGGAAATAG TAAAATCCAA AAGGGCGAGC 
ACAACTACTA CAAAAGTGGA AAACATAACA AGAGTAGGAA CTTCTGCAAC ATGTTGTAAT 
TGTGGTGAAG AATTGACTAA AATATGTTCA CTTCAGTTAT GTGATGACTG TTTAGAGAAA 
AGAAGCACCA CAACCTTATC ATTTCTCATA AAGAAATTAA AGAGACAAAA AGAATACCAA 
ACACTAAAGA CCGTGTGCAG GACGTGCAGT TATCGTTACA CTTCCGATGC AGGCATCGAA 
AATGACCATA TAGCTAGTAA ATGCAATTCA TATGACTGTC CAGTATTTTA CTCTCGTGTC 
AAAGCAGAAA GATACTTGAG AGATAATCAA TCTGTTCAAA GGGAAGAAGC ATTAATATCT 
CTAAATGATT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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REV3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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