SSO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SSO1
Entrez Gene ID	855844
Full Name	syntaxin
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184046.1	NP_015092.1	syntaxin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	STX1A	XP_001146754.2
Bos taurus (cattle)	STX1A	NP_001077267.1
Mus musculus (house mouse)	Stx1a	NP_058081.2
Rattus norvegicus (Norway rat)	Stx1a	NP_446240.2
Caenorhabditis elegans (roundworm)	unc-64	NP_001022615.1
Saccharomyces cerevisiae (baker's yeast)	SSO2	NP_013908.1
Neurospora crassa	NCU04244	XP_961235.1
Kluyveromyces lactis	KLLA0C15961g	XP_452913.1
Arabidopsis thaliana (thale cress)	SYP132	NP_568187.1
Xenopus tropicalis (tropical clawed frog)	stx1a	NP_001072191.1
Saccharomyces cerevisiae (baker's yeast)	SSO1	NP_015092.1
Homo sapiens (human)	STX1A	NP_004594.1
	STX1A	NP_001028037.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007698	XP_564525.2
Schizosaccharomyces pombe (fission yeast)	psy1	NP_588053.1
Magnaporthe oryzae (rice blast fungus)	MGG_00978	XP_003717971.1
Arabidopsis thaliana (thale cress)	SYP131	NP_187030.1
Canis lupus familiaris (dog)	STX1A	XP_005621021.1
Drosophila melanogaster (fruit fly)	Syx1A	NP_001189276.1
Eremothecium gossypii	AGOS_AFL139W	NP_985411.1
Oryza sativa (rice)	Os06g0168500	NP_001056925.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The shape of the exoplasmic hemi-transmembrane domain, in addition to the length of the entire transmembrane domain, determine retention in the Golgi or exit to the plasma membrane of SFT1 and SSO1 proteins.
Title: Short transmembrane domains with high-volume exoplasmic halves determine retention of Type II membrane proteins in the Golgi complex.

The Sso1, but not SSo2, is essential for prospore membrane formation.
Title: Phosphatidylinositol-4,5-bisphosphate and phospholipase D-generated phosphatidic acid specify SNARE-mediated vesicle fusion for prospore membrane formation.

EPR analysis of spin-labeled mutants showed that the transmembrane domain of Sso1p is a well-defined membrane spanning alpha-helix.
Title: Transmembrane organization of yeast syntaxin-analogue Sso1p.

Data show that the required function of the NH2-terminal regulatory domain (NRD) of the yeast plasma membrane syntaxin, Sso1p, can be circumvented when t-SNARE complex formation is made intramolecular.
Title: An intramolecular t-SNARE complex functions in vivo without the syntaxin NH2-terminal regulatory domain.

The following SSO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04725	NM_001184046.1 Latest version!	Saccharomyces cerevisiae S288C syntaxin (SSO1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SSO1 ( NM_001184046.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015092.1 Saccharomyces cerevisiae S288C syntaxin (SSO1), partial mRNA.

Price & Availability↑

CloneID	OSi04725
Clone ID Related Accession (Same CDS sequence)	NM_001184046.1
Accession Version	NM_001184046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	syntaxin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTTATA ATAATCCGTA CCAGTTGGAA ACCCCTTTTG AAGAGTCATA CGAGTTGGAC 
GAAGGTTCGA GCGCTATCGG TGCTGAAGGC CACGATTTCG TGGGCTTCAT GAATAAGATC 
AGTCAAATCA ATCGCGATCT CGATAAGTAC GACCATACCA TCAACCAGGT CGATTCTTTG 
CATAAGAGGC TACTGACCGA AGTTAATGAG GAGCAAGCAA GTCACTTAAG GCACTCCCTG 
GACAACTTCG TCGCACAAGC CACGGACTTG CAGTTCAAAC TGAAAAATGA GATTAAAAGT 
GCCCAAAGGG ATGGGATACA TGACACCAAC AAGCAAGCTC AGGCGGAAAA CTCCAGACAA 
AGATTTTTGA AGCTTATCCA GGACTACAGA ATTGTGGATT CCAACTACAA GGAGGAGAAT 
AAAGAGCAAG CCAAGAGGCA GTATATGATC ATTCAACCAG AGGCCACCGA AGATGAAGTT 
GAAGCAGCCA TAAGCGATGT AGGGGGCCAG CAGATCTTCT CACAAGCATT GTTGAATGCT 
AACAGACGTG GGGAAGCCAA GACTGCTCTT GCGGAAGTCC AGGCAAGGCA CCAAGAGTTA 
TTGAAACTAG AAAAATCCAT GGCAGAACTT ACTCAATTGT TTAATGACAT GGAAGAACTG 
GTAATAGAAC AACAAGAAAA CGTAGACGTC ATCGACAAGA ACGTTGAAGA CGCTCAACTC 
GACGTAGAAC AGGGTGTCGG TCATACCGAT AAAGCCGTCA AGAGTGCCAG AAAAGCAAGA 
AAGAACAAGA TTAGATGTTG GTTGATTGTA TTCGCCATCA TTGTAGTCGT TGTTGTTGTC 
GTTGTTGTCC CAGCCGTTGT CAAAACGCGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015092.1
CDS	1..873
Translation	MSYNNPYQLETPFEESYELDEGSSAIGAEGHDFVGFMNKISQINRDLDKYDHTINQVDSLHKRLLTEVNEEQASHLRHSLDNFVAQATDLQFKLKNEIKSAQRDGIHDTNKQAQAENSRQRFLKLIQDYRIVDSNYKEENKEQAKRQYMIIQPEATEDEVEAAISDVGGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKSMAELTQLFNDMEELVIEQQENVDVIDKNVEDAQLDVEQGVGHTDKAVKSARKARKNKIRCWLIVFAIIVVVVVVVVVPAVVKTR

Target ORF information:

RefSeq Version	NM_001184046.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C syntaxin (SSO1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184046.1

ORF Insert Sequence:

ATGAGTTATA ATAATCCGTA CCAGTTGGAA ACCCCTTTTG AAGAGTCATA CGAGTTGGAC 
GAAGGTTCGA GCGCTATCGG TGCTGAAGGC CACGATTTCG TGGGCTTCAT GAATAAGATC 
AGTCAAATCA ATCGCGATCT CGATAAGTAC GACCATACCA TCAACCAGGT CGATTCTTTG 
CATAAGAGGC TACTGACCGA AGTTAATGAG GAGCAAGCAA GTCACTTAAG GCACTCCCTG 
GACAACTTCG TCGCACAAGC CACGGACTTG CAGTTCAAAC TGAAAAATGA GATTAAAAGT 
GCCCAAAGGG ATGGGATACA TGACACCAAC AAGCAAGCTC AGGCGGAAAA CTCCAGACAA 
AGATTTTTGA AGCTTATCCA GGACTACAGA ATTGTGGATT CCAACTACAA GGAGGAGAAT 
AAAGAGCAAG CCAAGAGGCA GTATATGATC ATTCAACCAG AGGCCACCGA AGATGAAGTT 
GAAGCAGCCA TAAGCGATGT AGGGGGCCAG CAGATCTTCT CACAAGCATT GTTGAATGCT 
AACAGACGTG GGGAAGCCAA GACTGCTCTT GCGGAAGTCC AGGCAAGGCA CCAAGAGTTA 
TTGAAACTAG AAAAATCCAT GGCAGAACTT ACTCAATTGT TTAATGACAT GGAAGAACTG 
GTAATAGAAC AACAAGAAAA CGTAGACGTC ATCGACAAGA ACGTTGAAGA CGCTCAACTC 
GACGTAGAAC AGGGTGTCGG TCATACCGAT AAAGCCGTCA AGAGTGCCAG AAAAGCAAGA 
AAGAACAAGA TTAGATGTTG GTTGATTGTA TTCGCCATCA TTGTAGTCGT TGTTGTTGTC 
GTTGTTGTCC CAGCCGTTGT CAAAACGCGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SSO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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