ORF » Species Summary » Saccharomyces cerevisiae S288C » LEU4 cDNA ORF clone

LEU4 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following LEU4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LEU4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04528 NM_001182942.1
Latest version!		 Saccharomyces cerevisiae S288C 2-isopropylmalate synthase LEU4 (LEU4), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LEU4 ( NM_001182942.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014295.1 Saccharomyces cerevisiae S288C 2-isopropylmalate synthase LEU4 (LEU4), partial mRNA.
    Price & Availability↑
    CloneID OSi04528
    Clone ID Related Accession (Same CDS sequence) NM_001182942.1
    Accession Version NM_001182942.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product 2-isopropylmalate synthase LEU4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGTTAAAG AGAGTATTAT TGCTCTTGCT GAGCATGCGG CCTCCAGAGC CTCAAGAGTT 
    ATTCCTCCAG TGAAGCTAGC CTATAAAAAT ATGCTTAAGG ACCCTTCCTC CAAGTACAAG 
    CCATTTAACG CTCCAAAGCT ATCTAATAGA AAGTGGCCGG ATAACCGGAT CACGAGGGCT 
    CCTCGTTGGT TATCAACAGA TTTGAGAGAT GGTAACCAAT CTCTGCCGGA TCCCATGTCA 
    GTGGAACAAA AGAAAGAATA CTTTCACAAG CTGGTCAATA TTGGGTTCAA AGAAATCGAG 
    GTTTCCTTCC CCTCTGCATC TCAAACAGAT TTCGACTTCA CTAGATATGC TGTAGAAAAC 
    GCCCCAGACG ATGTTAGTAT TCAATGTCTT GTCCAATCTA GAGAACACTT GATTAAGAGA 
    ACGGTGGAAG CATTAACAGG TGCTAAAAAG GCTACTATAC ATACTTACTT GGCAACAAGT 
    GATATGTTCC GTGAAATTGT TTTTAATATG TCTAGAGAGG AAGCTATTTC CAAGGCAGTA 
    GAGGCCACCA AACTAGTTAG GAAACTAACT AAGGATGACC CTTCCCAACA AGCCACTCGT 
    TGGTCCTATG AGTTTTCCCC CGAATGTTTC AGTGATACTC CAGGTGAATT TGCTGTAGAA 
    ATTTGCGAAG CTGTTAAGAA GGCTTGGGAA CCTACCGAGG AAAATCCAAT CATTTTCAAC 
    TTACCTGCTA CCGTAGAAGT TGCCTCTCCA AATGTTTATG CTGATCAGAT TGAATACTTC 
    GCTACCCATA TTACTGAGCG TGAGAAGGTT TGCATCTCTA CACATTGTCA CAATGACCGT 
    GGTTGCGGTG TCGCCGCCAC AGAGTTAGGT ATGCTTGCAG GTGCCGACCG TGTAGAAGGA 
    TGTCTCTTTG GTAATGGTGA ACGTACAGGT AATGTGGACT TGGTTACTGT TGCTATGAAT 
    ATGTATACCC AAGGTGTTTC TCCTAATTTG GATTTCTCTG ACTTGACCTC TGTCCTAGAT 
    GTGGTTGAGC GTTGTAATAA GATCCCAGTA TCGCAAAGAG CACCATACGG CGGTGACTTG 
    GTCGTTTGTG CCTTTTCCGG TTCTCACCAA GACGCCATTA AGAAGGGTTT CAACTTACAA 
    AACAAGAAGC GTGCTCAAGG TGAAACTCAA TGGAGAATCC CATACTTGCC ATTGGATCCA 
    AAGGACATTG GCCGTGATTA CGAAGCTGTC ATCAGAGTCA ACTCTCAGTC TGGTAAAGGT 
    GGTGCCGCTT GGGTTATTTT GAGATCTTTG GGTTTGGATC TACCAAGAAA CATGCAAATC 
    GAATTTTCTA GCGCCGTTCA AGACCATGCT GACTCCTTGG GTAGAGAACT AAAATCAGAT 
    GAGATTTCCA AGTTATTCAA AGAGGCTTAC AACTACAATG ACGAACAGTA CCAAGCTATT 
    AGTTTAGTCA ATTATAATGT TGAAAAATTC GGCACTGAAC GTAGAGTGTT CACTGGTCAA 
    GTCAAAGTAG GCGACCAGAT CGTCGATATT GAAGGTACAG GTAATGGTCC AATCTCTTCT 
    TTGGTCGACG CCCTATCAAA CTTGTTGAAC GTGAGATTTG CCGTAGCAAA CTACACAGAG 
    CATTCTCTAG GTTCTGGTTC TTCTACGCAA GCTGCTTCTT ACATCCATCT ATCGTATAGG 
    CGTAATGCCG ACAACGAAAA GGCCTACAAA TGGGGTGTAG GTGTCTCCGA AGATGTCGGT 
    GATTCTTCAG TGAGAGCCAT CTTTGCCACC ATTAACAATA TTATCCATTC TGGTGATGTG 
    TCCATTCCAT CTTTGGCCGA GGTCGAAGGT AAGAATGCTG CGGCATCTGG CTCTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014295.1
    CDS1..1860
    Translation

    Target ORF information:

    RefSeq Version NM_001182942.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C 2-isopropylmalate synthase LEU4 (LEU4), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001182942.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGTTAAAG AGAGTATTAT TGCTCTTGCT GAGCATGCGG CCTCCAGAGC CTCAAGAGTT 
    ATTCCTCCAG TGAAGCTAGC CTATAAAAAT ATGCTTAAGG ACCCTTCCTC CAAGTACAAG 
    CCATTTAACG CTCCAAAGCT ATCTAATAGA AAGTGGCCGG ATAACCGGAT CACGAGGGCT 
    CCTCGTTGGT TATCAACAGA TTTGAGAGAT GGTAACCAAT CTCTGCCGGA TCCCATGTCA 
    GTGGAACAAA AGAAAGAATA CTTTCACAAG CTGGTCAATA TTGGGTTCAA AGAAATCGAG 
    GTTTCCTTCC CCTCTGCATC TCAAACAGAT TTCGACTTCA CTAGATATGC TGTAGAAAAC 
    GCCCCAGACG ATGTTAGTAT TCAATGTCTT GTCCAATCTA GAGAACACTT GATTAAGAGA 
    ACGGTGGAAG CATTAACAGG TGCTAAAAAG GCTACTATAC ATACTTACTT GGCAACAAGT 
    GATATGTTCC GTGAAATTGT TTTTAATATG TCTAGAGAGG AAGCTATTTC CAAGGCAGTA 
    GAGGCCACCA AACTAGTTAG GAAACTAACT AAGGATGACC CTTCCCAACA AGCCACTCGT 
    TGGTCCTATG AGTTTTCCCC CGAATGTTTC AGTGATACTC CAGGTGAATT TGCTGTAGAA 
    ATTTGCGAAG CTGTTAAGAA GGCTTGGGAA CCTACCGAGG AAAATCCAAT CATTTTCAAC 
    TTACCTGCTA CCGTAGAAGT TGCCTCTCCA AATGTTTATG CTGATCAGAT TGAATACTTC 
    GCTACCCATA TTACTGAGCG TGAGAAGGTT TGCATCTCTA CACATTGTCA CAATGACCGT 
    GGTTGCGGTG TCGCCGCCAC AGAGTTAGGT ATGCTTGCAG GTGCCGACCG TGTAGAAGGA 
    TGTCTCTTTG GTAATGGTGA ACGTACAGGT AATGTGGACT TGGTTACTGT TGCTATGAAT 
    ATGTATACCC AAGGTGTTTC TCCTAATTTG GATTTCTCTG ACTTGACCTC TGTCCTAGAT 
    GTGGTTGAGC GTTGTAATAA GATCCCAGTA TCGCAAAGAG CACCATACGG CGGTGACTTG 
    GTCGTTTGTG CCTTTTCCGG TTCTCACCAA GACGCCATTA AGAAGGGTTT CAACTTACAA 
    AACAAGAAGC GTGCTCAAGG TGAAACTCAA TGGAGAATCC CATACTTGCC ATTGGATCCA 
    AAGGACATTG GCCGTGATTA CGAAGCTGTC ATCAGAGTCA ACTCTCAGTC TGGTAAAGGT 
    GGTGCCGCTT GGGTTATTTT GAGATCTTTG GGTTTGGATC TACCAAGAAA CATGCAAATC 
    GAATTTTCTA GCGCCGTTCA AGACCATGCT GACTCCTTGG GTAGAGAACT AAAATCAGAT 
    GAGATTTCCA AGTTATTCAA AGAGGCTTAC AACTACAATG ACGAACAGTA CCAAGCTATT 
    AGTTTAGTCA ATTATAATGT TGAAAAATTC GGCACTGAAC GTAGAGTGTT CACTGGTCAA 
    GTCAAAGTAG GCGACCAGAT CGTCGATATT GAAGGTACAG GTAATGGTCC AATCTCTTCT 
    TTGGTCGACG CCCTATCAAA CTTGTTGAAC GTGAGATTTG CCGTAGCAAA CTACACAGAG 
    CATTCTCTAG GTTCTGGTTC TTCTACGCAA GCTGCTTCTT ACATCCATCT ATCGTATAGG 
    CGTAATGCCG ACAACGAAAA GGCCTACAAA TGGGGTGTAG GTGTCTCCGA AGATGTCGGT 
    GATTCTTCAG TGAGAGCCAT CTTTGCCACC ATTAACAATA TTATCCATTC TGGTGATGTG 
    TCCATTCCAT CTTTGGCCGA GGTCGAAGGT AAGAATGCTG CGGCATCTGG CTCTGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
    Nature387(6632 Suppl)93-8(1997 May)
    Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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