SUI1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
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Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The GTPase-activating protein eIF5 and beta-subunit of eIF2 promote transcriptional accuracy by controlling eIF1 dissociation and the stability of ternary complex binding to the preinitiation complex.
Title: Enhanced eIF1 binding to the 40S ribosome impedes conformational rearrangements of the preinitiation complex and elevates initiation accuracy.

substituting Lys-60 in helix alpha1, or either Lys-37 or Arg-33 in beta-hairpin loop-1, impairs binding of yeast eIF1 to 40 S.eIF1A complexes in vitro, and it confers increased initiation at UUG codons (Sui(-) phenotype)
Title: ?-Hairpin loop of eukaryotic initiation factor 1 (eIF1) mediates 40 S ribosome binding to regulate initiator tRNA(Met) recruitment and accuracy of AUG selection in vivo.

eIF1 autoregulates translation in yeast and discrimination against poor sequence context in vivo depends on specific domains and residues in eIF1, eIF1A, and eIF2beta
Title: Functional elements in initiation factors 1, 1A, and 2? discriminate against poor AUG context and non-AUG start codons.

Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo.
Title: Dissociation of eIF1 from the 40S ribosomal subunit is a key step in start codon selection in vivo.

Our results suggest that eIF1 and eIF1A promote an open, scanning-competent preinitiation complex that closes upon start codon recognition and eIF1 release to stabilize ternary complex binding and clamp down on mRNA.
Title: The eukaryotic translation initiation factors eIF1 and eIF1A induce an open conformation of the 40S ribosome.