RRP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RRP5
Entrez Gene ID	855269
Full Name	Rrp5p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182736.1	NP_013956.1	Rrp5p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B08239g	XP_451897.1
Schizosaccharomyces pombe (fission yeast)	SPCC1183.07	NP_587890.1
Eremothecium gossypii	AGOS_AFR566C	NP_986113.2
Magnaporthe oryzae (rice blast fungus)	MGG_05260	XP_003712817.1
Saccharomyces cerevisiae (baker's yeast)	RRP5	NP_013956.1
Neurospora crassa	NCU06272	XP_962876.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that ATP-bound DEAD-box protein Rok1, but not adenosine diphosphate (ADP)-bound Rok1, stabilizes Rrp5 protein binding to 40S ribosomes.
Title: The DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation.

Rrp5 provides specificity to the otherwise nonspecific biochemical activities of the Rok1 DEAD-domain.
Title: Cofactor-dependent specificity of a DEAD-box protein.

The results show that the large ribosomal subunit (LSU) biogenesis factor Noc1p of Saccharomyces cerevisiae can simultaneously interact with the LSU biogenesis factor Noc2p and Rrp5p.
Title: Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae.

association of Rrp5p with the pre-ribosome, Rrp5 binds to the RNA site with single-stranded U stretches
Title: Rrp5p, a trans-acting factor in yeast ribosome biogenesis, is an RNA-binding protein with a pronounced preference for U-rich sequences.

three distal S1 motifs of Saccharomyces cerevisiae Rrp5p have roles in pre-rRNA processing at site A(2) but not in production of functional 40S subunits
Title: Deletion of the three distal S1 motifs of Saccharomyces cerevisiae Rrp5p abolishes pre-rRNA processing at site A(2) without reducing the production of functional 40S subunits.

The following RRP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RRP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04224	NM_001182736.1 Latest version!	Saccharomyces cerevisiae S288C Rrp5p (RRP5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RRP5 ( NM_001182736.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013956.1 Saccharomyces cerevisiae S288C Rrp5p (RRP5), partial mRNA.

Price & Availability↑

CloneID	OSi04224
Clone ID Related Accession (Same CDS sequence)	NM_001182736.1
Accession Version	NM_001182736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Rrp5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTAGCTT CCACCAAAAG AAAGAGAGAT GAAGATTTTC CACTTTCGAG GGAAGACTCC 
ACAAAGCAGC CCTCTACCTC ATCATTAGTA AGGAACACTG AGGAAGTCTC TTTTCCAAGG 
GGTGGTGCGT CTGCTTTGAC TCCTTTAGAA CTGAAACAAG TTGCTAACGA AGCTGCAAGT 
GATGTGTTAT TTGGTAATGA AAGTGTCAAA GCATCTGAAC CAGCAAGTAG ACCCTTGAAA 
AAGAAGAAGA CAACAAAGAA ATCCACTTCT AAAGATTCCG AGGCCTCATC AGCAAACTCT 
GATGAAGCTA GAGCTGGACT AATTGAACAT GTCAACTTTA AAACGTTGAA GAATGGCTCT 
TCTTTGCTTG GTCAGATTTC TGCTATCACT AAACAAGATT TATGTATAAC ATTCACAGAC 
GGAATTTCTG GTTATGTGAA CCTAACACAT ATCTCAGAAG AATTTACTTC GATTTTGGAA 
GATCTGGATG AAGATATGGA TAGTGATACT GATGCTGCTG ATGAGAAAAA ATCCAAAGTT 
GAAGATGCAG AATACGAATC GTCCGACGAC GAAGATGAAA AATTAGATAA ATCTAATGAA 
TTACCTAATT TAAGAAGGTA CTTTCACATT GGTCAATGGC TAAGATGCTC TGTCATCAAG 
AATACCTCTT TAGAACCATC AACTAAGAAA TCCAAAAAGA AGAGAATAGA GTTAACCATC 
GAACCTTCAT CCGTCAACAT ATACGCAGAT GAAGATTTGG TTAAATCCAC GTCTATTCAA 
TGCGCGGTCA AATCCATCGA GGATCACGGT GCTACCTTAG ATGTTGGTCT GCCAGGCTTT 
ACAGGGTTCA TTGCTAAGAA GGATTTTGGC AATTTTGAAA AATTATTGCC GGGGGCTGTT 
TTCTTAGGTA ATATCACCAA AAAATCCGAC AGATCAATTG TTGTAAATAC AGACTTTTCG 
GACAAGAAAA ACAAAATTAC TCAAATCTCC TCAATTGATG CCATTATCCC TGGTCAAATC 
GTCGACTTAT TGTGTGAATC AATCACTAAA AATGGTATTG CCGGTAAAGT TTTTGGGTTA 
GTATCCGGTG TTGTCAACGT ATCACATTTA AGAACATTTT CCGAAGAAGA TTTAAAGCAT 
AAATTTGTTA TTGGTTCTAG TATTAGATGC CGAATTATTG CTTGTTTAGA AAACAAAAGC 
GGTGACAAGG TCTTGATTTT GTCAAACCTT CCACACATTT TAAAACTAGA AGACGCTTTG 
AGATCTACTG AAGGTCTTGA TGCATTCCCT ATCGGCTACA CATTTGAATC ATGCAGCATT 
AAAGGGCGTG ATTCCGAATA TCTATACTTA GCATTGGATG ATGATAGATT AGGTAAAGTA 
CATTCTTCAA GAGTTGGTGA AATTGAAAAT TCAGAAAATT TAAGTTCCAG GGTCCTTGGT 
TATAGCCCTG TTGATGACAT TTATCAATTG TCCACTGATC CAAAATACCT AAAATTAAAG 
TATCTAAGAA CAAATGATAT TCCAATCGGA GAATTATTAC CAAGTTGTGA AATAACTTCC 
GTCTCTAGTT CAGGTATTGA ATTGAAGATA TTTAACGGGC AATTTAAGGC TTCTGTTCCA 
CCATTACACA TTTCAGATAC CAGGCTAGTT TATCCAGAAA GGAAGTTCAA GATAGGTTCT 
AAGGTTAAAG GTAGAGTTAT ATCAGTTAAT TCAAGAGGTA ATGTGCATGT CACTTTGAAA 
AAATCATTAG TTAATATTGA AGACAACGAA TTACCACTGG TTTCCACTTA TGAAAACGCC 
AAAAATATCA AAGAAAAAAA TGAAAAGACT TTAGCAACTA TTCAAGTTTT CAAACCAAAT 
GGTTGCATCA TTTCCTTTTT TGGTGGTTTA TCGGGATTTT TACCTAATTC TGAGATCTCA 
GAAGTCTTTG TGAAGAGACC AGAAGAACAT TTAAGACTCG GTCAAACAGT AATAGTAAAA 
CTTCTTGACG TCGATGCTGA CAGGAGGAGA ATTATTGCCA CATGCAAAGT TTCAAATGAA 
CAGGCTGCCC AACAAAAAGA TACCATTGAA AATATTGTTC CAGGCAGAAC AATCATTACT 
GTCCATGTTA TCGAAAAAAC CAAAGATTCT GTTATTGTTG AAATTCCTGA TGTTGGCTTG 
CGTGGTGTCA TCTACGTTGG TCATCTCTCT GATTCCAGAA TTGAACAAAA TAGGGCTCAG 
TTGAAAAAGC TCAGAATCGG CACTGAACTG ACTGGTTTGG TAATTGATAA AGATACAAGA 
ACCCGCGTTT TCAATATGTC CCTCAAAAGT TCTCTGATTA AAGACGCTAA AAAGGAGACT 
CTTCCATTAA CATATGATGA TGTTAAGGAT CTAAATAAAG ATGTACCAAT GCACGCTTAC 
ATTAAATCCA TTTCTGATAA GGGTCTCTTT GTTGCATTTA ACGGTAAGTT CATTGGGTTG 
GTTTTACCAA GTTACGCTGT TGATAGTAGG GATATTGATA TCAGCAAGGC GTTTTATATT 
AACCAATCCG TTACCGTCTA TTTATTAAGA ACAGACGACA AAAACCAGAA ATTCCTATTA 
TCTCTAAAAG CTCCAAAGGT CAAAGAAGAG AAGAAGAAAG TTGAATCTAA TATCGAAGAT 
CCTGTTGATT CGTCTATCAA AAGCTGGGAC GATTTGTCTA TTGGTAGTAT TGTTAAAGCA 
AAAATCAAGA GCGTCAAGAA AAATCAATTG AATGTTATTT TAGCGGCAAA TTTGCATGGC 
AGAGTGGACA TTGCTGAAGT GTTTGACACG TATGAAGAAA TTACAGATAA GAAACAGCCG 
TTGAGTAATT ACAAAAAAGA CGATGTCATC AAGGTAAAAA TCATAGGTAA TCACGATGTC 
AAGAGCCATA AGTTCTTGCC GATTACACAC AAAATCTCAA AGGCTAGCGT TTTGGAATTA 
TCCATGAAAC CATCAGAATT AAAATCTAAG GAAGTGCACA CAAAAAGTCT TGAGGAAATC 
AACATTGGGC AAGAGTTAAC TGGCTTTGTC AACAATTCAT CAGGTAACCA CCTATGGCTA 
ACAATTTCTC CAGTTTTGAA GGCGAGAATT TCTCTACTAG ATTTAGCTGA TAACGATTCA 
AATTTTTCAG AAAATATTGA ATCTGTTTTC CCATTAGGTT CAGCCCTCCA AGTGAAAGTT 
GCCTCTATTG ACCGCGAGCA TGGCTTTGTC AATGCCATTG GAAAGAGTCA TGTTGACATA 
AATATGTCCA CAATTAAAGT TGGTGATGAA TTGCCAGGTC GTGTTCTAAA AATAGCTGAA 
AAGTATGTTT TGTTAGACCT TGGTAACAAA GTGACTGGTA TTTCCTTTAT TACGGATGCG 
TTAAATGATT TCTCCCTAAC ATTGAAGGAA GCTTTTGAAG ACAAAATTAA CAACGTCATT 
CCAACTACCG TTTTATCTGT TGATGAACAG AATAAGAAAA TTGAACTATC CCTTCGTCCT 
GCTACTGCAA AAACTCGTTC GATCAAATCT CATGAAGATT TGAAGCAAGG CGAGATTGTA 
GATGGTATTG TTAAGAATGT GAATGACAAA GGTATTTTTG TTTACTTGAG TAGGAAAGTT 
GAAGCCTTTG TCCCTGTTAG TAAACTATCT GATTCTTACT TGAAGGAATG GAAGAAGTTT 
TACAAGCCTA TGCAATACGT TTTGGGAAAA GTCGTTACAT GCGATGAAGA TTCTCGTATT 
TCTCTAACCC TAAGAGAATC CGAAATAAAT GGTGATTTAA AGGTTCTAAA GACCTATTCA 
GACATTAAGG CTGGTGATGT TTTCGAGGGT ACAATTAAAA GCGTCACTGA TTTTGGTGTT 
TTCGTCAAAC TAGATAACAC TGTTAATGTT ACTGGTTTGG CACATATTAC TGAAATTGCG 
GACAAAAAAC CTGAAGATCT TTCTGCCTTG TTTGGTGTTG GGGACAGAGT AAAAGCTATT 
GTTTTGAAAA CAAATCCAGA AAAAAAACAA ATTTCATTGA GCTTGAAAGC GTCTCATTTC 
TCTAAAGAAG CCGAATTGGC TTCTACTACC ACCACCACTA CTACTGTGGA TCAACTGGAA 
AAGGAGGATG AAGATGAAGT CATGGCAGAT GCTGGCTTCA ACGATTCCGA CAGCGAATCT 
GATATCGGAG ACCAAAACAC CGAAGTTGCC GATAGGAAGC CTGAAACATC TTCGGATGGG 
CTATCCTTGA GTGCTGGTTT TGATTGGACT GCTTCCATTT TGGATCAAGC GCAAGAAGAA 
GAAGAGTCTG ACCAAGATCA AGAAGACTTT ACTGAAAATA AGAAACACAA GCACAAGAGA 
AGAAAGGAAA ATGTTGTTCA GGATAAGACC ATTGATATCA ACACTAGGGC ACCAGAATCT 
GTTGCCGATT TTGAACGTCT ACTTATCGGT AATCCAAATT CGTCCGTAGT GTGGATGAAC 
TATATGGCTT TCCAACTACA ACTGAGTGAG ATCGAGAAGG CTCGAGAGCT CGCAGAACGT 
GCGCTAAAGA CCATCAACTT CAGAGAAGAA GCCGAGAAGT TGAATATTTG GATTGCAATG 
CTGAATTTAG AAAATACTTT TGGTACCGAA GAAACTTTAG AAGAAGTGTT TAGCAGGGCT 
TGTCAGTATA TGGACTCATA TACTATTCAT ACAAAACTGC TTGGTATTTA TGAAATAAGT 
GAAAAGTTCG ACAAAGCAGC AGAATTATTC AAAGCTACAG CTAAGAAATT TGGCGGCGAG 
AAAGTTTCTA TCTGGGTATC GTGGGGTGAT TTTTTGATCA GCCACAATGA AGAGCAAGAG 
GCACGTACTA TTTTAGGTAA TGCTTTGAAG GCCCTACCAA AGCGTAATCA TATCGAGGTT 
GTCCGCAAGT TTGCTCAATT AGAATTTGCA AAGGGTGACC CAGAAAGGGG TCGGTCATTA 
TTCGAAGGTT TGGTTGCTGA TGCGCCAAAG AGAATTGACC TGTGGAACGT TTACGTAGAC 
CAGGAAGTGA AGGCAAAAGA TAAGAAGAAG GTGGAAGATT TATTTGAGAG AATAATAACC 
AAAAAAATAA CAAGGAAGCA GGCTAAGTTT TTCTTTAATA AATGGTTACA ATTTGAGGAA 
TCTGAAGGTG ACGAAAAGAC CATTGAATAC GTTAAAGCTA AAGCTACTGA GTATGTCGCT 
AGCCATGAAT CTCAAAAAGC AGACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013956.1
CDS	1..5190
Translation	MVASTKRKRDEDFPLSREDSTKQPSTSSLVRNTEEVSFPRGGASALTPLELKQVANEAASDVLFGNESVKASEPASRPLKKKKTTKKSTSKDSEASSANSDEARAGLIEHVNFKTLKNGSSLLGQISAITKQDLCITFTDGISGYVNLTHISEEFTSILEDLDEDMDSDTDAADEKKSKVEDAEYESSDDEDEKLDKSNELPNLRRYFHIGQWLRCSVIKNTSLEPSTKKSKKKRIELTIEPSSVNIYADEDLVKSTSIQCAVKSIEDHGATLDVGLPGFTGFIAKKDFGNFEKLLPGAVFLGNITKKSDRSIVVNTDFSDKKNKITQISSIDAIIPGQIVDLLCESITKNGIAGKVFGLVSGVVNVSHLRTFSEEDLKHKFVIGSSIRCRIIACLENKSGDKVLILSNLPHILKLEDALRSTEGLDAFPIGYTFESCSIKGRDSEYLYLALDDDRLGKVHSSRVGEIENSENLSSRVLGYSPVDDIYQLSTDPKYLKLKYLRTNDIPIGELLPSCEITSVSSSGIELKIFNGQFKASVPPLHISDTRLVYPERKFKIGSKVKGRVISVNSRGNVHVTLKKSLVNIEDNELPLVSTYENAKNIKEKNEKTLATIQVFKPNGCIISFFGGLSGFLPNSEISEVFVKRPEEHLRLGQTVIVKLLDVDADRRRIIATCKVSNEQAAQQKDTIENIVPGRTIITVHVIEKTKDSVIVEIPDVGLRGVIYVGHLSDSRIEQNRAQLKKLRIGTELTGLVIDKDTRTRVFNMSLKSSLIKDAKKETLPLTYDDVKDLNKDVPMHAYIKSISDKGLFVAFNGKFIGLVLPSYAVDSRDIDISKAFYINQSVTVYLLRTDDKNQKFLLSLKAPKVKEEKKKVESNIEDPVDSSIKSWDDLSIGSIVKAKIKSVKKNQLNVILAANLHGRVDIAEVFDTYEEITDKKQPLSNYKKDDVIKVKIIGNHDVKSHKFLPITHKISKASVLELSMKPSELKSKEVHTKSLEEINIGQELTGFVNNSSGNHLWLTISPVLKARISLLDLADNDSNFSENIESVFPLGSALQVKVASIDREHGFVNAIGKSHVDINMSTIKVGDELPGRVLKIAEKYVLLDLGNKVTGISFITDALNDFSLTLKEAFEDKINNVIPTTVLSVDEQNKKIELSLRPATAKTRSIKSHEDLKQGEIVDGIVKNVNDKGIFVYLSRKVEAFVPVSKLSDSYLKEWKKFYKPMQYVLGKVVTCDEDSRISLTLRESEINGDLKVLKTYSDIKAGDVFEGTIKSVTDFGVFVKLDNTVNVTGLAHITEIADKKPEDLSALFGVGDRVKAIVLKTNPEKKQISLSLKASHFSKEAELASTTTTTTTVDQLEKEDEDEVMADAGFNDSDSESDIGDQNTEVADRKPETSSDGLSLSAGFDWTASILDQAQEEEESDQDQEDFTENKKHKHKRRKENVVQDKTIDINTRAPESVADFERLLIGNPNSSVVWMNYMAFQLQLSEIEKARELAERALKTINFREEAEKLNIWIAMLNLENTFGTEETLEEVFSRACQYMDSYTIHTKLLGIYEISEKFDKAAELFKATAKKFGGEKVSIWVSWGDFLISHNEEQEARTILGNALKALPKRNHIEVVRKFAQLEFAKGDPERGRSLFEGLVADAPKRIDLWNVYVDQEVKAKDKKKVEDLFERIITKKITRKQAKFFFNKWLQFEESEGDEKTIEYVKAKATEYVASHESQKADE

Target ORF information:

RefSeq Version	NM_001182736.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Rrp5p (RRP5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182736.1

ORF Insert Sequence:

ATGGTAGCTT CCACCAAAAG AAAGAGAGAT GAAGATTTTC CACTTTCGAG GGAAGACTCC 
ACAAAGCAGC CCTCTACCTC ATCATTAGTA AGGAACACTG AGGAAGTCTC TTTTCCAAGG 
GGTGGTGCGT CTGCTTTGAC TCCTTTAGAA CTGAAACAAG TTGCTAACGA AGCTGCAAGT 
GATGTGTTAT TTGGTAATGA AAGTGTCAAA GCATCTGAAC CAGCAAGTAG ACCCTTGAAA 
AAGAAGAAGA CAACAAAGAA ATCCACTTCT AAAGATTCCG AGGCCTCATC AGCAAACTCT 
GATGAAGCTA GAGCTGGACT AATTGAACAT GTCAACTTTA AAACGTTGAA GAATGGCTCT 
TCTTTGCTTG GTCAGATTTC TGCTATCACT AAACAAGATT TATGTATAAC ATTCACAGAC 
GGAATTTCTG GTTATGTGAA CCTAACACAT ATCTCAGAAG AATTTACTTC GATTTTGGAA 
GATCTGGATG AAGATATGGA TAGTGATACT GATGCTGCTG ATGAGAAAAA ATCCAAAGTT 
GAAGATGCAG AATACGAATC GTCCGACGAC GAAGATGAAA AATTAGATAA ATCTAATGAA 
TTACCTAATT TAAGAAGGTA CTTTCACATT GGTCAATGGC TAAGATGCTC TGTCATCAAG 
AATACCTCTT TAGAACCATC AACTAAGAAA TCCAAAAAGA AGAGAATAGA GTTAACCATC 
GAACCTTCAT CCGTCAACAT ATACGCAGAT GAAGATTTGG TTAAATCCAC GTCTATTCAA 
TGCGCGGTCA AATCCATCGA GGATCACGGT GCTACCTTAG ATGTTGGTCT GCCAGGCTTT 
ACAGGGTTCA TTGCTAAGAA GGATTTTGGC AATTTTGAAA AATTATTGCC GGGGGCTGTT 
TTCTTAGGTA ATATCACCAA AAAATCCGAC AGATCAATTG TTGTAAATAC AGACTTTTCG 
GACAAGAAAA ACAAAATTAC TCAAATCTCC TCAATTGATG CCATTATCCC TGGTCAAATC 
GTCGACTTAT TGTGTGAATC AATCACTAAA AATGGTATTG CCGGTAAAGT TTTTGGGTTA 
GTATCCGGTG TTGTCAACGT ATCACATTTA AGAACATTTT CCGAAGAAGA TTTAAAGCAT 
AAATTTGTTA TTGGTTCTAG TATTAGATGC CGAATTATTG CTTGTTTAGA AAACAAAAGC 
GGTGACAAGG TCTTGATTTT GTCAAACCTT CCACACATTT TAAAACTAGA AGACGCTTTG 
AGATCTACTG AAGGTCTTGA TGCATTCCCT ATCGGCTACA CATTTGAATC ATGCAGCATT 
AAAGGGCGTG ATTCCGAATA TCTATACTTA GCATTGGATG ATGATAGATT AGGTAAAGTA 
CATTCTTCAA GAGTTGGTGA AATTGAAAAT TCAGAAAATT TAAGTTCCAG GGTCCTTGGT 
TATAGCCCTG TTGATGACAT TTATCAATTG TCCACTGATC CAAAATACCT AAAATTAAAG 
TATCTAAGAA CAAATGATAT TCCAATCGGA GAATTATTAC CAAGTTGTGA AATAACTTCC 
GTCTCTAGTT CAGGTATTGA ATTGAAGATA TTTAACGGGC AATTTAAGGC TTCTGTTCCA 
CCATTACACA TTTCAGATAC CAGGCTAGTT TATCCAGAAA GGAAGTTCAA GATAGGTTCT 
AAGGTTAAAG GTAGAGTTAT ATCAGTTAAT TCAAGAGGTA ATGTGCATGT CACTTTGAAA 
AAATCATTAG TTAATATTGA AGACAACGAA TTACCACTGG TTTCCACTTA TGAAAACGCC 
AAAAATATCA AAGAAAAAAA TGAAAAGACT TTAGCAACTA TTCAAGTTTT CAAACCAAAT 
GGTTGCATCA TTTCCTTTTT TGGTGGTTTA TCGGGATTTT TACCTAATTC TGAGATCTCA 
GAAGTCTTTG TGAAGAGACC AGAAGAACAT TTAAGACTCG GTCAAACAGT AATAGTAAAA 
CTTCTTGACG TCGATGCTGA CAGGAGGAGA ATTATTGCCA CATGCAAAGT TTCAAATGAA 
CAGGCTGCCC AACAAAAAGA TACCATTGAA AATATTGTTC CAGGCAGAAC AATCATTACT 
GTCCATGTTA TCGAAAAAAC CAAAGATTCT GTTATTGTTG AAATTCCTGA TGTTGGCTTG 
CGTGGTGTCA TCTACGTTGG TCATCTCTCT GATTCCAGAA TTGAACAAAA TAGGGCTCAG 
TTGAAAAAGC TCAGAATCGG CACTGAACTG ACTGGTTTGG TAATTGATAA AGATACAAGA 
ACCCGCGTTT TCAATATGTC CCTCAAAAGT TCTCTGATTA AAGACGCTAA AAAGGAGACT 
CTTCCATTAA CATATGATGA TGTTAAGGAT CTAAATAAAG ATGTACCAAT GCACGCTTAC 
ATTAAATCCA TTTCTGATAA GGGTCTCTTT GTTGCATTTA ACGGTAAGTT CATTGGGTTG 
GTTTTACCAA GTTACGCTGT TGATAGTAGG GATATTGATA TCAGCAAGGC GTTTTATATT 
AACCAATCCG TTACCGTCTA TTTATTAAGA ACAGACGACA AAAACCAGAA ATTCCTATTA 
TCTCTAAAAG CTCCAAAGGT CAAAGAAGAG AAGAAGAAAG TTGAATCTAA TATCGAAGAT 
CCTGTTGATT CGTCTATCAA AAGCTGGGAC GATTTGTCTA TTGGTAGTAT TGTTAAAGCA 
AAAATCAAGA GCGTCAAGAA AAATCAATTG AATGTTATTT TAGCGGCAAA TTTGCATGGC 
AGAGTGGACA TTGCTGAAGT GTTTGACACG TATGAAGAAA TTACAGATAA GAAACAGCCG 
TTGAGTAATT ACAAAAAAGA CGATGTCATC AAGGTAAAAA TCATAGGTAA TCACGATGTC 
AAGAGCCATA AGTTCTTGCC GATTACACAC AAAATCTCAA AGGCTAGCGT TTTGGAATTA 
TCCATGAAAC CATCAGAATT AAAATCTAAG GAAGTGCACA CAAAAAGTCT TGAGGAAATC 
AACATTGGGC AAGAGTTAAC TGGCTTTGTC AACAATTCAT CAGGTAACCA CCTATGGCTA 
ACAATTTCTC CAGTTTTGAA GGCGAGAATT TCTCTACTAG ATTTAGCTGA TAACGATTCA 
AATTTTTCAG AAAATATTGA ATCTGTTTTC CCATTAGGTT CAGCCCTCCA AGTGAAAGTT 
GCCTCTATTG ACCGCGAGCA TGGCTTTGTC AATGCCATTG GAAAGAGTCA TGTTGACATA 
AATATGTCCA CAATTAAAGT TGGTGATGAA TTGCCAGGTC GTGTTCTAAA AATAGCTGAA 
AAGTATGTTT TGTTAGACCT TGGTAACAAA GTGACTGGTA TTTCCTTTAT TACGGATGCG 
TTAAATGATT TCTCCCTAAC ATTGAAGGAA GCTTTTGAAG ACAAAATTAA CAACGTCATT 
CCAACTACCG TTTTATCTGT TGATGAACAG AATAAGAAAA TTGAACTATC CCTTCGTCCT 
GCTACTGCAA AAACTCGTTC GATCAAATCT CATGAAGATT TGAAGCAAGG CGAGATTGTA 
GATGGTATTG TTAAGAATGT GAATGACAAA GGTATTTTTG TTTACTTGAG TAGGAAAGTT 
GAAGCCTTTG TCCCTGTTAG TAAACTATCT GATTCTTACT TGAAGGAATG GAAGAAGTTT 
TACAAGCCTA TGCAATACGT TTTGGGAAAA GTCGTTACAT GCGATGAAGA TTCTCGTATT 
TCTCTAACCC TAAGAGAATC CGAAATAAAT GGTGATTTAA AGGTTCTAAA GACCTATTCA 
GACATTAAGG CTGGTGATGT TTTCGAGGGT ACAATTAAAA GCGTCACTGA TTTTGGTGTT 
TTCGTCAAAC TAGATAACAC TGTTAATGTT ACTGGTTTGG CACATATTAC TGAAATTGCG 
GACAAAAAAC CTGAAGATCT TTCTGCCTTG TTTGGTGTTG GGGACAGAGT AAAAGCTATT 
GTTTTGAAAA CAAATCCAGA AAAAAAACAA ATTTCATTGA GCTTGAAAGC GTCTCATTTC 
TCTAAAGAAG CCGAATTGGC TTCTACTACC ACCACCACTA CTACTGTGGA TCAACTGGAA 
AAGGAGGATG AAGATGAAGT CATGGCAGAT GCTGGCTTCA ACGATTCCGA CAGCGAATCT 
GATATCGGAG ACCAAAACAC CGAAGTTGCC GATAGGAAGC CTGAAACATC TTCGGATGGG 
CTATCCTTGA GTGCTGGTTT TGATTGGACT GCTTCCATTT TGGATCAAGC GCAAGAAGAA 
GAAGAGTCTG ACCAAGATCA AGAAGACTTT ACTGAAAATA AGAAACACAA GCACAAGAGA 
AGAAAGGAAA ATGTTGTTCA GGATAAGACC ATTGATATCA ACACTAGGGC ACCAGAATCT 
GTTGCCGATT TTGAACGTCT ACTTATCGGT AATCCAAATT CGTCCGTAGT GTGGATGAAC 
TATATGGCTT TCCAACTACA ACTGAGTGAG ATCGAGAAGG CTCGAGAGCT CGCAGAACGT 
GCGCTAAAGA CCATCAACTT CAGAGAAGAA GCCGAGAAGT TGAATATTTG GATTGCAATG 
CTGAATTTAG AAAATACTTT TGGTACCGAA GAAACTTTAG AAGAAGTGTT TAGCAGGGCT 
TGTCAGTATA TGGACTCATA TACTATTCAT ACAAAACTGC TTGGTATTTA TGAAATAAGT 
GAAAAGTTCG ACAAAGCAGC AGAATTATTC AAAGCTACAG CTAAGAAATT TGGCGGCGAG 
AAAGTTTCTA TCTGGGTATC GTGGGGTGAT TTTTTGATCA GCCACAATGA AGAGCAAGAG 
GCACGTACTA TTTTAGGTAA TGCTTTGAAG GCCCTACCAA AGCGTAATCA TATCGAGGTT 
GTCCGCAAGT TTGCTCAATT AGAATTTGCA AAGGGTGACC CAGAAAGGGG TCGGTCATTA 
TTCGAAGGTT TGGTTGCTGA TGCGCCAAAG AGAATTGACC TGTGGAACGT TTACGTAGAC 
CAGGAAGTGA AGGCAAAAGA TAAGAAGAAG GTGGAAGATT TATTTGAGAG AATAATAACC 
AAAAAAATAA CAAGGAAGCA GGCTAAGTTT TTCTTTAATA AATGGTTACA ATTTGAGGAA 
TCTGAAGGTG ACGAAAAGAC CATTGAATAC GTTAAAGCTA AAGCTACTGA GTATGTCGCT 
AGCCATGAAT CTCAAAAAGC AGACGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RRP5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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