PAH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PAH1
Entrez Gene ID	855201
Full Name	phosphatidate phosphatase PAH1
Synonyms	SMP2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182669.1	NP_013888.1	phosphatidate phosphatase PAH1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	PAH1	NP_013888.1
Eremothecium gossypii	AGOS_AFL195W	NP_985355.1
Schizosaccharomyces pombe (fission yeast)	ned1	NP_594815.1
Magnaporthe oryzae (rice blast fungus)	MGG_01311	XP_003714235.1
Neurospora crassa	NCU03137	XP_964186.2
Kluyveromyces lactis	KLLA0D09867g	XP_453500.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Site-specific mutational analyses of the conserved residues within WRDPLVDID indicated that Trp-637 plays a crucial role in Pah1 function. This work also demonstrated that the catalytic activity of Pah1 is required but is not sufficient for its in vivo functions
Title: A conserved tryptophan within the WRDPLVDID domain of yeast Pah1 phosphatidate phosphatase is required for its

These results suggest that Pah1p plays an important role in vacuolar activity by negatively regulating the expression of V-ATPase genes. As such, we provide evidence to show the role of Pah1p in vacuolar acidification and fragmentation.
Title: Pah1p negatively regulates the expression of V-ATPase genes as well as vacuolar acidification.

The Pkc1-mediated degradation of Pah1 was attenuated in the cho1Delta mutant, which is deficient in PS synthase, supporting the notion that the phospholipid regulates Pkc1 activity in vivo.
Title: Phosphorylation of lipid metabolic enzymes by yeast protein kinase C requires phosphatidylserine and diacylglycerol.

The expression of Pah1 with combined mutations of S705D and 7A, which mimic its phosphorylation by CKII and lack of phosphorylation by Pho85-Pho80, caused an increase in triacylglycerol content and lipid droplet number in cells expressing the Nem1-Spo7 phosphatase complex.
Title: Phosphorylation of Yeast Pah1 Phosphatidate Phosphatase by Casein Kinase II Regulates Its Function in Lipid Metabolism.

shortened chronological life span of the pah1Delta mutant along with its growth defect on non-fermentable carbon sources and hypersensitivity to hydrogen peroxide was suppressed by the loss of Dgk1 diacylglycerol kinase
Title: Altered Lipid Synthesis by Lack of Yeast Pah1 Phosphatidate Phosphatase Reduces Chronological Life Span.

The following PAH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04160	NM_001182669.1 Latest version!	Saccharomyces cerevisiae S288C phosphatidate phosphatase PAH1 (PAH1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAH1 ( NM_001182669.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013888.1 Saccharomyces cerevisiae S288C phosphatidate phosphatase PAH1 (PAH1), partial mRNA.

Price & Availability↑

CloneID	OSi04160
Clone ID Related Accession (Same CDS sequence)	NM_001182669.1
Accession Version	NM_001182669.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphatidate phosphatase PAH1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCAGTACG TAGGCAGAGC TCTTGGGTCT GTGTCTAAAA CATGGTCTTC TATCAATCCG 
GCTACGCTAT CAGGTGCTAT AGATGTCATT GTAGTGGAGC ATCCAGACGG AAGGCTATCA 
TGTTCTCCCT TTCATGTGAG GTTCGGCAAA TTTCAAATTC TAAAGCCATC TCAAAAGAAA 
GTCCAAGTGT TTATAAATGA GAAACTGAGT AATATGCCAA TGAAACTGAG TGATTCTGGA 
GAAGCCTATT TCGTTTTCGA GATGGGTGAC CAGGTCACTG ATGTCCCTGA CGAATTGCTT 
GTGTCGCCCG TGATGAGCGC CACATCAAGC CCCCCTCAAT CACCTGAAAC ATCCATCTTA 
GAAGGAGGAA CCGAGGGTGA AGGTGAAGGT GAAAATGAAA ATAAGAAGAA GGAAAAGAAA 
GTGCTAGAGG AACCAGATTT TTTAGATATC AATGACACTG GAGATTCAGG CAGTAAAAAT 
AGTGAAACTA CAGGGTCGCT TTCTCCTACT GAATCCTCTA CAACGACACC ACCAGATTCA 
GTTGAAGAGA GGAAGCTTGT TGAGCAGCGT ACAAAGAACT TTCAGCAAAA ACTAAACAAA 
AAACTCACTG AAATCCATAT ACCCAGTAAA CTTGATAACA ATGGCGACTT ACTACTAGAC 
ACTGAAGGTT ACAAGCCAAA CAAGAATATG ATGCATGACA CAGACATACA ACTGAAGCAG 
TTGTTAAAGG ACGAATTCGG TAATGATTCA GATATTTCCA GTTTTATCAA GGAGGACAAA 
AATGGCAACA TCAAGATCGT AAATCCTTAC GAGCACCTTA CTGATTTATC TCCTCCAGGT 
ACGCCTCCAA CAATGGCCAC AAGCGGATCA GTTTTAGGCT TAGATGCAAT GGAATCAGGA 
AGTACTTTGA ATTCGTTATC TTCTTCACCT TCTGGTTCCG ATACTGAGGA CGAAACATCA 
TTTAGCAAAG AACAAAGCAG TAAAAGTGAA AAAACTAGCA AGAAAGGAAC AGCAGGGAGC 
GGTGAGACCG AGAAAAGATA CATACGAACG ATAAGATTGA CTAATGACCA GTTAAAGTGC 
CTAAATTTAA CTTATGGTGA AAATGATCTG AAATTTTCCG TAGATCACGG AAAAGCTATT 
GTTACGTCAA AATTATTCGT TTGGAGGTGG GATGTTCCAA TTGTTATCAG TGATATTGAT 
GGCACCATCA CAAAATCGGA CGCTTTAGGC CATGTTCTGG CAATGATAGG AAAAGACTGG 
ACGCACTTGG GTGTAGCCAA GTTATTTAGC GAGATCTCCA GGAATGGCTA TAATATACTC 
TATCTAACTG CAAGAAGTGC TGGACAAGCT GATTCCACGA GGAGTTATTT GCGATCAATT 
GAACAGAATG GCAGCAAACT ACCAAATGGG CCTGTGATTT TATCACCCGA TAGAACGATG 
GCTGCGTTAA GGCGGGAAGT AATACTAAAA AAACCTGAAG TCTTTAAAAT CGCGTGTCTA 
AACGACATAA GATCCTTGTA TTTTGAAGAC AGTGATAACG AAGTGGATAC AGAGGAAAAA 
TCAACACCAT TTTTTGCCGG CTTTGGTAAT AGGATTACTG ATGCTTTATC TTACAGAACT 
GTGGGGATAC CTAGTTCAAG AATTTTCACA ATAAATACAG AGGGTGAGGT TCATATGGAA 
TTATTGGAGT TAGCAGGTTA CAGAAGCTCC TATATTCATA TCAATGAGCT TGTCGATCAT 
TTCTTTCCAC CAGTCAGCCT TGATAGTGTC GATCTAAGAA CTAATACTTC CATGGTTCCT 
GGCTCCCCCC CTAATAGAAC GTTGGATAAC TTTGACTCAG AAATTACTTC AGGTCGCAAA 
ACGCTATTTA GAGGCAATCA GGAAGAGAAA TTCACAGACG TAAATTTTTG GAGAGACCCG 
TTAGTCGACA TCGACAACTT ATCGGATATT AGCAATGATG ATTCTGATAA CATCGATGAA 
GATACTGACG TATCACAACA AAGCAACATT AGTAGAAATA GGGCAAATTC AGTCAAAACC 
GCCAAGGTCA CTAAAGCCCC GCAAAGAAAT GTGAGCGGCA GCACAAATAA CAACGAAGTT 
TTAGCCGCTT CGTCTGATGT AGAAAATGCG TCTGACCTGG TGAGTTCCCA TAGTAGCTCA 
GGATCCACGC CCAATAAATC TACAATGTCC AAAGGGGACA TTGGAAAACA AATATATTTG 
GAGCTAGGTT CTCCACTTGC ATCGCCAAAA CTAAGATATT TAGACGATAT GGATGATGAA 
GACTCCAATT ACAATAGAAC TAAATCAAGG AGAGCATCTT CTGCAGCCGC GACTAGTATC 
GATAAAGAGT TCAAAAAGCT CTCTGTGTCA AAGGCCGGCG CTCCAACAAG AATTGTTTCA 
AAGATCAACG TTTCAAATGA CGTACATTCA CTTGGGAATT CAGATACCGA ATCACGAAGG 
GAGCAAAGTG TTAATGAAAC AGGGCGCAAT CAGCTACCCC ACAACTCAAT GGACGATAAA 
GATTTGGATT CAAGAGTAAG CGATGAATTC GATGACGATG AATTCGACGA AGATGAATTC 
GAAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013888.1
CDS	1..2589
Translation	MQYVGRALGSVSKTWSSINPATLSGAIDVIVVEHPDGRLSCSPFHVRFGKFQILKPSQKKVQVFINEKLSNMPMKLSDSGEAYFVFEMGDQVTDVPDELLVSPVMSATSSPPQSPETSILEGGTEGEGEGENENKKKEKKVLEEPDFLDINDTGDSGSKNSETTGSLSPTESSTTTPPDSVEERKLVEQRTKNFQQKLNKKLTEIHIPSKLDNNGDLLLDTEGYKPNKNMMHDTDIQLKQLLKDEFGNDSDISSFIKEDKNGNIKIVNPYEHLTDLSPPGTPPTMATSGSVLGLDAMESGSTLNSLSSSPSGSDTEDETSFSKEQSSKSEKTSKKGTAGSGETEKRYIRTIRLTNDQLKCLNLTYGENDLKFSVDHGKAIVTSKLFVWRWDVPIVISDIDGTITKSDALGHVLAMIGKDWTHLGVAKLFSEISRNGYNILYLTARSAGQADSTRSYLRSIEQNGSKLPNGPVILSPDRTMAALRREVILKKPEVFKIACLNDIRSLYFEDSDNEVDTEEKSTPFFAGFGNRITDALSYRTVGIPSSRIFTINTEGEVHMELLELAGYRSSYIHINELVDHFFPPVSLDSVDLRTNTSMVPGSPPNRTLDNFDSEITSGRKTLFRGNQEEKFTDVNFWRDPLVDIDNLSDISNDDSDNIDEDTDVSQQSNISRNRANSVKTAKVTKAPQRNVSGSTNNNEVLAASSDVENASDLVSSHSSSGSTPNKSTMSKGDIGKQIYLELGSPLASPKLRYLDDMDDEDSNYNRTKSRRASSAAATSIDKEFKKLSVSKAGAPTRIVSKINVSNDVHSLGNSDTESRREQSVNETGRNQLPHNSMDDKDLDSRVSDEFDDDEFDEDEFED

Target ORF information:

RefSeq Version	NM_001182669.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphatidate phosphatase PAH1 (PAH1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182669.1

ORF Insert Sequence:

ATGCAGTACG TAGGCAGAGC TCTTGGGTCT GTGTCTAAAA CATGGTCTTC TATCAATCCG 
GCTACGCTAT CAGGTGCTAT AGATGTCATT GTAGTGGAGC ATCCAGACGG AAGGCTATCA 
TGTTCTCCCT TTCATGTGAG GTTCGGCAAA TTTCAAATTC TAAAGCCATC TCAAAAGAAA 
GTCCAAGTGT TTATAAATGA GAAACTGAGT AATATGCCAA TGAAACTGAG TGATTCTGGA 
GAAGCCTATT TCGTTTTCGA GATGGGTGAC CAGGTCACTG ATGTCCCTGA CGAATTGCTT 
GTGTCGCCCG TGATGAGCGC CACATCAAGC CCCCCTCAAT CACCTGAAAC ATCCATCTTA 
GAAGGAGGAA CCGAGGGTGA AGGTGAAGGT GAAAATGAAA ATAAGAAGAA GGAAAAGAAA 
GTGCTAGAGG AACCAGATTT TTTAGATATC AATGACACTG GAGATTCAGG CAGTAAAAAT 
AGTGAAACTA CAGGGTCGCT TTCTCCTACT GAATCCTCTA CAACGACACC ACCAGATTCA 
GTTGAAGAGA GGAAGCTTGT TGAGCAGCGT ACAAAGAACT TTCAGCAAAA ACTAAACAAA 
AAACTCACTG AAATCCATAT ACCCAGTAAA CTTGATAACA ATGGCGACTT ACTACTAGAC 
ACTGAAGGTT ACAAGCCAAA CAAGAATATG ATGCATGACA CAGACATACA ACTGAAGCAG 
TTGTTAAAGG ACGAATTCGG TAATGATTCA GATATTTCCA GTTTTATCAA GGAGGACAAA 
AATGGCAACA TCAAGATCGT AAATCCTTAC GAGCACCTTA CTGATTTATC TCCTCCAGGT 
ACGCCTCCAA CAATGGCCAC AAGCGGATCA GTTTTAGGCT TAGATGCAAT GGAATCAGGA 
AGTACTTTGA ATTCGTTATC TTCTTCACCT TCTGGTTCCG ATACTGAGGA CGAAACATCA 
TTTAGCAAAG AACAAAGCAG TAAAAGTGAA AAAACTAGCA AGAAAGGAAC AGCAGGGAGC 
GGTGAGACCG AGAAAAGATA CATACGAACG ATAAGATTGA CTAATGACCA GTTAAAGTGC 
CTAAATTTAA CTTATGGTGA AAATGATCTG AAATTTTCCG TAGATCACGG AAAAGCTATT 
GTTACGTCAA AATTATTCGT TTGGAGGTGG GATGTTCCAA TTGTTATCAG TGATATTGAT 
GGCACCATCA CAAAATCGGA CGCTTTAGGC CATGTTCTGG CAATGATAGG AAAAGACTGG 
ACGCACTTGG GTGTAGCCAA GTTATTTAGC GAGATCTCCA GGAATGGCTA TAATATACTC 
TATCTAACTG CAAGAAGTGC TGGACAAGCT GATTCCACGA GGAGTTATTT GCGATCAATT 
GAACAGAATG GCAGCAAACT ACCAAATGGG CCTGTGATTT TATCACCCGA TAGAACGATG 
GCTGCGTTAA GGCGGGAAGT AATACTAAAA AAACCTGAAG TCTTTAAAAT CGCGTGTCTA 
AACGACATAA GATCCTTGTA TTTTGAAGAC AGTGATAACG AAGTGGATAC AGAGGAAAAA 
TCAACACCAT TTTTTGCCGG CTTTGGTAAT AGGATTACTG ATGCTTTATC TTACAGAACT 
GTGGGGATAC CTAGTTCAAG AATTTTCACA ATAAATACAG AGGGTGAGGT TCATATGGAA 
TTATTGGAGT TAGCAGGTTA CAGAAGCTCC TATATTCATA TCAATGAGCT TGTCGATCAT 
TTCTTTCCAC CAGTCAGCCT TGATAGTGTC GATCTAAGAA CTAATACTTC CATGGTTCCT 
GGCTCCCCCC CTAATAGAAC GTTGGATAAC TTTGACTCAG AAATTACTTC AGGTCGCAAA 
ACGCTATTTA GAGGCAATCA GGAAGAGAAA TTCACAGACG TAAATTTTTG GAGAGACCCG 
TTAGTCGACA TCGACAACTT ATCGGATATT AGCAATGATG ATTCTGATAA CATCGATGAA 
GATACTGACG TATCACAACA AAGCAACATT AGTAGAAATA GGGCAAATTC AGTCAAAACC 
GCCAAGGTCA CTAAAGCCCC GCAAAGAAAT GTGAGCGGCA GCACAAATAA CAACGAAGTT 
TTAGCCGCTT CGTCTGATGT AGAAAATGCG TCTGACCTGG TGAGTTCCCA TAGTAGCTCA 
GGATCCACGC CCAATAAATC TACAATGTCC AAAGGGGACA TTGGAAAACA AATATATTTG 
GAGCTAGGTT CTCCACTTGC ATCGCCAAAA CTAAGATATT TAGACGATAT GGATGATGAA 
GACTCCAATT ACAATAGAAC TAAATCAAGG AGAGCATCTT CTGCAGCCGC GACTAGTATC 
GATAAAGAGT TCAAAAAGCT CTCTGTGTCA AAGGCCGGCG CTCCAACAAG AATTGTTTCA 
AAGATCAACG TTTCAAATGA CGTACATTCA CTTGGGAATT CAGATACCGA ATCACGAAGG 
GAGCAAAGTG TTAATGAAAC AGGGCGCAAT CAGCTACCCC ACAACTCAAT GGACGATAAA 
GATTTGGATT CAAGAGTAAG CGATGAATTC GATGACGATG AATTCGACGA AGATGAATTC 
GAAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PAH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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