NAM7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	NAM7
Entrez Gene ID	855104
Full Name	ATP-dependent RNA helicase NAM7
Synonyms	IFS2,MOF4,SUP113,SUT2,UPF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182579.1	NP_013797.1	ATP-dependent RNA helicase NAM7

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UPF1	XP_512972.4
Mus musculus (house mouse)	Upf1	NP_001116301.1
Eremothecium gossypii	AGOS_ABR022C	NP_982968.4
	LOC720288	XP_002808229.1
Canis lupus familiaris (dog)	UPF1	XP_005632737.1
Bos taurus (cattle)	UPF1	XP_005208537.1
Saccharomyces cerevisiae (baker's yeast)	NAM7	NP_013797.1
Schizosaccharomyces pombe (fission yeast)	upf1	NP_593080.1
Magnaporthe oryzae (rice blast fungus)	MGG_00976	XP_003717973.1
Gallus gallus (chicken)	UPF1	XP_418237.3
Danio rerio (zebrafish)	upf1	NP_998639.1
Drosophila melanogaster (fruit fly)	Upf1	NP_572767.1
Oryza sativa (rice)	Os07g0495900	NP_001059698.1
Homo sapiens (human)	UPF1	NP_002902.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001133	XP_322028.4
Caenorhabditis elegans (roundworm)	smg-2	NP_490829.1
Kluyveromyces lactis	KLLA0B06435g	XP_451821.1
Neurospora crassa	NCU04242	XP_961233.1
Rattus norvegicus (Norway rat)	Upf1	XP_003751614.1
Arabidopsis thaliana (thale cress)	LBA1	NP_199512.2
Xenopus tropicalis (tropical clawed frog)	upf1	XP_002938872.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B
Nature387(6632 Suppl)90-3(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The propose that the Sse1/Hsp70 complex maintains the solubility of FLAG-Pgk1-300, thereby stimulating its Upf-dependent degradation by the proteasomes.
Title: Crucial role of ATP-bound Sse1 in Upf1-dependent degradation of the truncated product.

The study provides evidence that two tyrosines within a phospho-motif of Upf1 act redundantly to promote ATP hydrolysis, Nonsense-Mediated mRNA Decay efficiency and translation termination fidelity.
Title: Identification and functional analysis of novel phosphorylation sites in the RNA surveillance protein Upf1.

The cysteine- and histidine-rich (CH) domain and the RNA helicase domain of yeast Upf1 can engage in two new types of molecular interactions: an intramolecular interaction between these two domains and self-association of each of these domains.
Title: Intra- and intermolecular regulatory interactions in Upf1, the RNA helicase central to nonsense-mediated mRNA decay in yeast.

The association of Upf1 with the Rps26 40S subunit is consistent with the notion that this RNA helicase not only triggers rapid decay of nonsense-containing mRNAs, but may also have a role in dissociation of the premature termination complex.
Title: Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit.

The upf1Delta mutant contained elevated copper levels in the vacuole relative to wild-type yeast cells, after both strains were exposed to elevated copper levels. Copper tolerance by the upf1Delta mutant is dependent on the presence of CTR2.
Title: Physiological basis of copper tolerance of Saccharomyces cerevisiae nonsense-mediated mRNA decay mutants.

The following NAM7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAM7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04078	NM_001182579.1 Latest version!	Saccharomyces cerevisiae S288C ATP-dependent RNA helicase NAM7 (NAM7), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAM7 ( NM_001182579.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013797.1 Saccharomyces cerevisiae S288C ATP-dependent RNA helicase NAM7 (NAM7), partial mRNA.

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CloneID	OSi04078
Clone ID Related Accession (Same CDS sequence)	NM_001182579.1
Accession Version	NM_001182579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ATP-dependent RNA helicase NAM7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTCGGTT CCGGTTCTCA CACTCCTTAT GATATATCAA ACTCTCCATC TGATGTAAAT 
GTCCAACCCG CAACACAACT AAATTCCACC TTGGTGGAGG ATGACGATGT AGATAATCAG 
CTATTTGAAG AGGCTCAAGT CACTGAGACT GGATTCCGTT CGCCTTCAGC TTCAGACAAT 
TCATGTGCGT ATTGTGGTAT AGATTCTGCA AAGTGTGTCA TCAAATGTAA TTCATGTAAG 
AAATGGTTTT GTAACACTAA AAACGGTACA AGCAGCTCCC ACATTGTTAA TCACTTAGTT 
TTATCCCACC ATAACGTAGT TTCTTTACAT CCAGATTCTG ACTTAGGGGA TACCGTTTTG 
GAATGTTATA ACTGTGGACG TAAGAACGTG TTTTTATTGG GATTTGTTTC CGCTAAAAGT 
GAGGCCGTGG TTGTTTTACT TTGTAGAATA CCTTGTGCCC AGACGAAAAA TGCGAACTGG 
GATACTGATC AATGGCAACC ATTAATTGAA GACAGACAAC TTTTATCATG GGTCGCAGAG 
CAACCAACTG AAGAAGAAAA ATTGAAAGCT CGTTTAATCA CTCCTAGCCA AATTTCCAAG 
TTGGAGGCAA AATGGAGATC CAATAAAGAC GCTACAATTA ATGATATTGA CGCCCCAGAG 
GAACAGGAAG CAATCCCACC TTTACTATTG AGATATCAAG ACGCCTACGA ATACCAAAGA 
TCTTACGGGC CTTTAATCAA ATTGGAGGCC GACTATGATA AACAACTCAA GGAATCTCAA 
GCTTTAGAAC ATATTTCTGT TTCATGGTCC TTAGCTTTAA ATAATAGGCA TTTAGCATCT 
TTCACTTTAT CTACTTTCGA ATCTAACGAG TTGAAAGTTG CCATCGGTGA TGAAATGATA 
CTATGGTACT CTGGCATGCA ACATCCTGAT TGGGAAGGTC GTGGTTACAT TGTTCGGTTA 
CCAAATAGCT TCCAGGACAC ATTCACATTA GAGTTAAAAC CAAGTAAAAC GCCACCTCCA 
ACACATTTGA CCACTGGTTT TACTGCTGAG TTCATCTGGA AAGGTACCTC TTATGACAGG 
ATGCAAGACG CATTGAAAAA ATTTGCCATT GATAAAAAAT CTATTTCAGG TTATTTGTAC 
TATAAAATTT TAGGCCATCA AGTGGTTGAC ATTTCATTTG ATGTCCCATT ACCTAAGGAG 
TTTTCAATTC CGAATTTTGC ACAATTAAAC TCATCCCAGT CGAACGCTGT TAGTCATGTA 
TTACAACGTC CGTTATCTTT AATTCAAGGC CCACCAGGCA CTGGTAAAAC AGTTACTTCA 
GCAACGATTG TGTATCACCT TTCCAAAATA CACAAGGATA GAATATTGGT GTGTGCCCCA 
TCAAACGTTG CTGTAGATCA TTTGGCTGCC AAATTACGTG ACTTGGGTTT AAAAGTTGTT 
AGACTTACCG CGAAAAGTAG AGAAGATGTG GAGAGTTCCG TCTCCAACTT AGCATTGCAT 
AATTTGGTTG GCCGTGGTGC TAAAGGGGAA TTAAAAAACC TATTAAAGTT AAAGGATGAA 
GTTGGCGAAT TATCTGCTTC TGATACAAAA CGGTTTGTTA AATTAGTAAG GAAAACAGAA 
GCAGAAATTC TCAATAAGGC AGATGTCGTA TGTTGCACAT GTGTTGGTGC TGGTGATAAG 
CGCTTAGACA CTAAATTTAG GACTGTGTTA ATTGATGAAA GTACTCAAGC TTCTGAGCCG 
GAATGTTTAA TCCCAATCGT TAAAGGTGCG AAACAAGTTA TACTTGTTGG TGATCACCAG 
CAACTGGGCC CAGTCATATT GGAACGAAAG GCGGCAGACG CTGGTTTGAA ACAATCTCTC 
TTTGAAAGAT TAATCTCTCT AGGCCACGTA CCGATTCGTT TGGAAGTTCA ATACCGTATG 
AATCCTTATT TGAGTGAGTT TCCAAGTAAC ATGTTTTATG AAGGCAGCCT ACAAAATGGT 
GTAACGATTG AACAGCGTAC CGTTCCCAAC AGCAAATTCC CATGGCCAAT TCGCGGTATA 
CCAATGATGT TTTGGGCCAA TTACGGTAGA GAGGAGATTT CTGCTAACGG TACTTCCTTC 
TTAAACAGAA TTGAAGCCAT GAATTGTGAA CGAATCATCA CTAAACTTTT CAGAGACGGT 
GTCAAGCCCG AGCAAATTGG TGTTATCACA CCATATGAGG GACAAAGAGC TTATATTTTA 
CAATATATGC AAATGAATGG TTCATTGGAT AAGGATTTGT ATATCAAAGT GGAAGTTGCC 
TCAGTTGATG CATTCCAAGG TCGTGAAAAG GATTACATAA TCTTATCGTG TGTTCGTGCC 
AATGAACAAC AGGCCATTGG TTTCTTACGT GATCCTCGTC GTCTAAACGT GGGTCTAACC 
CGTGCCAAAT ATGGTCTAGT TATTCTTGGT AATCCTAGAT CTTTGGCAAG AAACACATTA 
TGGAACCATC TGTTAATCCA CTTCAGAGAG AAGGGTTGTT TAGTCGAAGG TACGTTGGAT 
AACTTACAGT TATGCACTGT TCAATTAGTT CGTCCTCAGC CAAGAAAGAC TGAACGGCCA 
ATGAACGCTC AATTTAACGT AGAATCTGAA ATGGGTGACT TTCCGAAGTT CCAGGATTTT 
GATGCACAGA GTATGGTGTC ATTCAGTGGT CAAATTGGGG ACTTTGGTAA TGCATTTGTT 
GACAACACAG AACTTTCTTC TTACATCAAT AATGAATATT GGAATTTTGA GAATTTTAAA 
AGTGCTTTTT CTCAAAAGCA AAATCGCAAT GAAATTGACG ATAGAAATTT GTACCAGGAG 
GAGGCTTCTC ATTTGAACTC TAACTTCGCG AGAGAGTTAC AGAGAGAAGA ACAAAAGCAT 
GAATTGTCAA AAGACTTCAG CAATTTGGGA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013797.1
CDS	1..2916
Translation	MVGSGSHTPYDISNSPSDVNVQPATQLNSTLVEDDDVDNQLFEEAQVTETGFRSPSASDNSCAYCGIDSAKCVIKCNSCKKWFCNTKNGTSSSHIVNHLVLSHHNVVSLHPDSDLGDTVLECYNCGRKNVFLLGFVSAKSEAVVVLLCRIPCAQTKNANWDTDQWQPLIEDRQLLSWVAEQPTEEEKLKARLITPSQISKLEAKWRSNKDATINDIDAPEEQEAIPPLLLRYQDAYEYQRSYGPLIKLEADYDKQLKESQALEHISVSWSLALNNRHLASFTLSTFESNELKVAIGDEMILWYSGMQHPDWEGRGYIVRLPNSFQDTFTLELKPSKTPPPTHLTTGFTAEFIWKGTSYDRMQDALKKFAIDKKSISGYLYYKILGHQVVDISFDVPLPKEFSIPNFAQLNSSQSNAVSHVLQRPLSLIQGPPGTGKTVTSATIVYHLSKIHKDRILVCAPSNVAVDHLAAKLRDLGLKVVRLTAKSREDVESSVSNLALHNLVGRGAKGELKNLLKLKDEVGELSASDTKRFVKLVRKTEAEILNKADVVCCTCVGAGDKRLDTKFRTVLIDESTQASEPECLIPIVKGAKQVILVGDHQQLGPVILERKAADAGLKQSLFERLISLGHVPIRLEVQYRMNPYLSEFPSNMFYEGSLQNGVTIEQRTVPNSKFPWPIRGIPMMFWANYGREEISANGTSFLNRIEAMNCERIITKLFRDGVKPEQIGVITPYEGQRAYILQYMQMNGSLDKDLYIKVEVASVDAFQGREKDYIILSCVRANEQQAIGFLRDPRRLNVGLTRAKYGLVILGNPRSLARNTLWNHLLIHFREKGCLVEGTLDNLQLCTVQLVRPQPRKTERPMNAQFNVESEMGDFPKFQDFDAQSMVSFSGQIGDFGNAFVDNTELSSYINNEYWNFENFKSAFSQKQNRNEIDDRNLYQEEASHLNSNFARELQREEQKHELSKDFSNLGI

Target ORF information:

RefSeq Version	NM_001182579.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ATP-dependent RNA helicase NAM7 (NAM7), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182579.1

ORF Insert Sequence:

ATGGTCGGTT CCGGTTCTCA CACTCCTTAT GATATATCAA ACTCTCCATC TGATGTAAAT 
GTCCAACCCG CAACACAACT AAATTCCACC TTGGTGGAGG ATGACGATGT AGATAATCAG 
CTATTTGAAG AGGCTCAAGT CACTGAGACT GGATTCCGTT CGCCTTCAGC TTCAGACAAT 
TCATGTGCGT ATTGTGGTAT AGATTCTGCA AAGTGTGTCA TCAAATGTAA TTCATGTAAG 
AAATGGTTTT GTAACACTAA AAACGGTACA AGCAGCTCCC ACATTGTTAA TCACTTAGTT 
TTATCCCACC ATAACGTAGT TTCTTTACAT CCAGATTCTG ACTTAGGGGA TACCGTTTTG 
GAATGTTATA ACTGTGGACG TAAGAACGTG TTTTTATTGG GATTTGTTTC CGCTAAAAGT 
GAGGCCGTGG TTGTTTTACT TTGTAGAATA CCTTGTGCCC AGACGAAAAA TGCGAACTGG 
GATACTGATC AATGGCAACC ATTAATTGAA GACAGACAAC TTTTATCATG GGTCGCAGAG 
CAACCAACTG AAGAAGAAAA ATTGAAAGCT CGTTTAATCA CTCCTAGCCA AATTTCCAAG 
TTGGAGGCAA AATGGAGATC CAATAAAGAC GCTACAATTA ATGATATTGA CGCCCCAGAG 
GAACAGGAAG CAATCCCACC TTTACTATTG AGATATCAAG ACGCCTACGA ATACCAAAGA 
TCTTACGGGC CTTTAATCAA ATTGGAGGCC GACTATGATA AACAACTCAA GGAATCTCAA 
GCTTTAGAAC ATATTTCTGT TTCATGGTCC TTAGCTTTAA ATAATAGGCA TTTAGCATCT 
TTCACTTTAT CTACTTTCGA ATCTAACGAG TTGAAAGTTG CCATCGGTGA TGAAATGATA 
CTATGGTACT CTGGCATGCA ACATCCTGAT TGGGAAGGTC GTGGTTACAT TGTTCGGTTA 
CCAAATAGCT TCCAGGACAC ATTCACATTA GAGTTAAAAC CAAGTAAAAC GCCACCTCCA 
ACACATTTGA CCACTGGTTT TACTGCTGAG TTCATCTGGA AAGGTACCTC TTATGACAGG 
ATGCAAGACG CATTGAAAAA ATTTGCCATT GATAAAAAAT CTATTTCAGG TTATTTGTAC 
TATAAAATTT TAGGCCATCA AGTGGTTGAC ATTTCATTTG ATGTCCCATT ACCTAAGGAG 
TTTTCAATTC CGAATTTTGC ACAATTAAAC TCATCCCAGT CGAACGCTGT TAGTCATGTA 
TTACAACGTC CGTTATCTTT AATTCAAGGC CCACCAGGCA CTGGTAAAAC AGTTACTTCA 
GCAACGATTG TGTATCACCT TTCCAAAATA CACAAGGATA GAATATTGGT GTGTGCCCCA 
TCAAACGTTG CTGTAGATCA TTTGGCTGCC AAATTACGTG ACTTGGGTTT AAAAGTTGTT 
AGACTTACCG CGAAAAGTAG AGAAGATGTG GAGAGTTCCG TCTCCAACTT AGCATTGCAT 
AATTTGGTTG GCCGTGGTGC TAAAGGGGAA TTAAAAAACC TATTAAAGTT AAAGGATGAA 
GTTGGCGAAT TATCTGCTTC TGATACAAAA CGGTTTGTTA AATTAGTAAG GAAAACAGAA 
GCAGAAATTC TCAATAAGGC AGATGTCGTA TGTTGCACAT GTGTTGGTGC TGGTGATAAG 
CGCTTAGACA CTAAATTTAG GACTGTGTTA ATTGATGAAA GTACTCAAGC TTCTGAGCCG 
GAATGTTTAA TCCCAATCGT TAAAGGTGCG AAACAAGTTA TACTTGTTGG TGATCACCAG 
CAACTGGGCC CAGTCATATT GGAACGAAAG GCGGCAGACG CTGGTTTGAA ACAATCTCTC 
TTTGAAAGAT TAATCTCTCT AGGCCACGTA CCGATTCGTT TGGAAGTTCA ATACCGTATG 
AATCCTTATT TGAGTGAGTT TCCAAGTAAC ATGTTTTATG AAGGCAGCCT ACAAAATGGT 
GTAACGATTG AACAGCGTAC CGTTCCCAAC AGCAAATTCC CATGGCCAAT TCGCGGTATA 
CCAATGATGT TTTGGGCCAA TTACGGTAGA GAGGAGATTT CTGCTAACGG TACTTCCTTC 
TTAAACAGAA TTGAAGCCAT GAATTGTGAA CGAATCATCA CTAAACTTTT CAGAGACGGT 
GTCAAGCCCG AGCAAATTGG TGTTATCACA CCATATGAGG GACAAAGAGC TTATATTTTA 
CAATATATGC AAATGAATGG TTCATTGGAT AAGGATTTGT ATATCAAAGT GGAAGTTGCC 
TCAGTTGATG CATTCCAAGG TCGTGAAAAG GATTACATAA TCTTATCGTG TGTTCGTGCC 
AATGAACAAC AGGCCATTGG TTTCTTACGT GATCCTCGTC GTCTAAACGT GGGTCTAACC 
CGTGCCAAAT ATGGTCTAGT TATTCTTGGT AATCCTAGAT CTTTGGCAAG AAACACATTA 
TGGAACCATC TGTTAATCCA CTTCAGAGAG AAGGGTTGTT TAGTCGAAGG TACGTTGGAT 
AACTTACAGT TATGCACTGT TCAATTAGTT CGTCCTCAGC CAAGAAAGAC TGAACGGCCA 
ATGAACGCTC AATTTAACGT AGAATCTGAA ATGGGTGACT TTCCGAAGTT CCAGGATTTT 
GATGCACAGA GTATGGTGTC ATTCAGTGGT CAAATTGGGG ACTTTGGTAA TGCATTTGTT 
GACAACACAG AACTTTCTTC TTACATCAAT AATGAATATT GGAATTTTGA GAATTTTAAA 
AGTGCTTTTT CTCAAAAGCA AAATCGCAAT GAAATTGACG ATAGAAATTT GTACCAGGAG 
GAGGCTTCTC ATTTGAACTC TAACTTCGCG AGAGAGTTAC AGAGAGAAGA ACAAAAGCAT 
GAATTGTCAA AAGACTTCAG CAATTTGGGA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
Nature387(6632 Suppl)90-3(1997 May)
Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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NAM7 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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