RPN2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RPN2
Entrez Gene ID	854735
Full Name	proteasome regulatory particle base subunit RPN2
Synonyms	SEN3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179425.1	NP_012190.1	proteasome regulatory particle base subunit RPN2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PSMD1	NP_001233478.1
Canis lupus familiaris (dog)	PSMD1	XP_003433398.1
Rattus norvegicus (Norway rat)	Psmd1	NP_114184.1
Saccharomyces cerevisiae (baker's yeast)	RPN2	NP_012190.1
	PSMD1	XP_001113035.1
Danio rerio (zebrafish)	psmd1	NP_957478.1
Drosophila melanogaster (fruit fly)	Rpn2	NP_001263057.1
Neurospora crassa	NCU09450	XP_958036.1
Arabidopsis thaliana (thale cress)	AT2G32730	NP_180832.1
Xenopus tropicalis (tropical clawed frog)	psmd1	NP_998867.1
Homo sapiens (human)	PSMD1	NP_002798.2
Mus musculus (house mouse)	Psmd1	NP_081633.1
Kluyveromyces lactis	KLLA0C09284g	XP_452613.1
Eremothecium gossypii	AGOS_ACL033C	NP_983371.1
Schizosaccharomyces pombe (fission yeast)	rpn2	NP_596381.1
Gallus gallus (chicken)	PSMD1	NP_001012618.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001745	XP_321340.5
Oryza sativa (rice)	Os08g0224700	NP_001061278.1
Bos taurus (cattle)	PSMD1	NP_001094547.1
Magnaporthe oryzae (rice blast fungus)	MGG_05991	XP_003711836.1
Arabidopsis thaliana (thale cress)	AT1G04810	NP_171973.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.
Title: Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.

Comparison of the full-length Rpn1 and Rpn2 with the truncated proteins showed molecules similar in size and shape, indicating that their terminal regions are flexible and thus smeared to invisibility in the averaged images.
Title: Electron microscopic evidence in support of alpha-solenoid models of proteasomal subunits Rpn1 and Rpn2.

Rpn2 interfaces with the 20S, whereas Rpn1 sits atop Rpn2, serving as a docking site for a substrate-recruitment factor. The 19S ATPases encircle the Rpn1-Rpn2 stack, covering the remainder of the 20S surface.
Title: The central unit within the 19S regulatory particle of the proteasome.

The following RPN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03763	NM_001179425.1 Latest version!	Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN2 (RPN2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPN2 ( NM_001179425.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012190.1 Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN2 (RPN2), partial mRNA.

Price & Availability↑

CloneID	OSi03763
Clone ID Related Accession (Same CDS sequence)	NM_001179425.1
Accession Version	NM_001179425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	proteasome regulatory particle base subunit RPN2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTTTGA CGACTGCTGC TCCTCTTTTA GCATTGTTGA GAGAGAATCA AGATTCTGTT 
AAAACATATG CCCTAGAATC TATAAATAAT GTTGTGGACC AATTATGGTC TGAGATCTCG 
AATGAACTGC CGGATATTGA GGCATTGTAT GATGACGATA CCTTTTCAGA CCGTGAAATG 
GCTGCTTTGA TTGCATCCAA AGTTTATTAC AATCTTGGTG AATACGAATC GGCTGTGAAG 
TATGCACTTG CCGCAAAGGA TCGTTTTGAT ATAGACGAAA AGTCACAATT TGTTGAAACT 
ATAGTCTCAA AGAGTATTGA GATGTACGTC CAAGAGGCTT CCAAGCAATA CACTAAGGAC 
GAACAATTTT ACACCAAGGA TATTATTGAC CCAAAATTAA CCTCTATTTT TGAGCGAATG 
ATAGAAAAAT GTTTGAAAGC TTCAGAATTA AAATTAGCTT TAGGAATTGC CTTAGAAGGT 
TACAGATTGG ATATCATTGA AAGTGCTTTA AAGAGCAAAT TGGATCAGGA TTCCACTTCT 
GAAAACGTTA AAATTATTAA CTACTTATTG ACCTTAGCTA TAACTACTGT GACAAATTCC 
AAGTTCAGAT CCTCGATATT GAGAAAATCA TTTGATTTCC TAATGAATAT GCCTAACTGC 
GATTACTTAA CGCTCAATAA AGTAGTTGTA AATTTGAATG ACGCCGGATT GGCACTTCAG 
CTATTTAAAA AATTAAAGGA AGAGAACGAT GAAGGATTAT CTGCTCAAAT TGCATTTGAT 
TTGGTTTCGT CTGCGTCTCA GCAGTTGCTT GAGATTTTAG TAACCGAACT AACTGCCCAG 
GGTTACGATC CTGCCTTATT GAATATTCTA TCTGGGCTAC CGACTTGTGA TTATTACAAT 
ACCTTCCTTT TGAATAATAA AAACATTGAC ATCGGTCTTT TAAACAAGTC TAAATCATCT 
TTGGATGGGA AATTTTCTTT GTTCCACACC GCAGTCAGCG TTGCCAACGG TTTTATGCAC 
GCTGGTACTA CCGACAATTC ATTTATCAAG GCGAACTTGC CATGGTTAGG TAAGGCTCAA 
AATTGGGCTA AATTTACAGC TACAGCCTCT TTAGGTGTAA TTCATAAAGG CAATTTATTG 
GAGGGTAAAA AAGTCATGGC CCCTTACTTG CCAGGCAGTC GAGCTTCTTC TAGGTTTATC 
AAAGGTGGTT CATTGTATGG TTTGGGTTTA ATTTATGCTG GCTTTGGTCG TGATACAACT 
GACTATCTGA AAAACATAAT AGTTGAGAAT AGCGGAACTT CAGGCGATGA AGACGTAGAT 
GTATTGTTAC ATGGTGCTTC ACTGGGTATC GGTCTTGCTG CTATGGGTTC TGCTAATATT 
GAAGTTTATG AAGCCTTAAA GGAAGTTCTG TACAACGATT CTGCCACATC TGGAGAAGCA 
GCTGCATTAG GTATGGGTCT CTGTATGCTC GGAACAGGTA AGCCTGAAGC GATTCACGAT 
ATGTTTACAT ATTCCCAAGA AACGCAGCAT GGCAATATTA CTCGTGGTCT AGCCGTTGGT 
TTAGCACTGA TAAATTACGG CCGTCAAGAG TTAGCTGATG ATTTAATTAC TAAGATGTTA 
GCAAGTGATG AGTCCTTGTT ACGCTATGGA GGTGCATTCA CAATCGCGTT AGCGTATGCG 
GGTACGGGAA ATAATTCAGC TGTCAAGAGA TTACTGCATG TGGCAGTCTC GGATTCTAAC 
GATGATGTCA GAAGGGCAGC AGTCATCGCA TTAGGGTTTG TTCTCTTGCG TGATTATACC 
ACTGTACCAA GGATTGTCCA ACTTCTATCT AAATCGCATA ACGCTCATGT CAGATGTGGT 
ACCGCATTTG CTCTTGGGAT TGCTTGTGCC GGCAAGGGTT TACAATCAGC TATTGACGTT 
TTAGATCCGC TAACAAAGGA CCCAGTAGAT TTTGTTCGTC AGGCTGCCAT GATAGCTTTA 
TCCATGATTT TGATTCAACA AACAGAAAAA CTAAACCCTC AAGTTGCTGA TATTAACAAA 
AATTTTTTGA GCGTGATTAC AAACAAACAC CAAGAAGGTT TAGCCAAATT CGGTGCATGT 
GTGGCCCAAG GTATAATGAA TGCCGGTGGG CGTAATGTTA CAATTCAGCT GGAAAATGCG 
GATACAGGCA CATTGGATAC CAAGTCCGTA GTTGGTTTGG TCATGTTCTC ACAATTCTGG 
TACTGGTTCC CACTGGCTCA CTTTTTATCC CTTTCTTTTA CACCAACAAC TGTCATTGGT 
ATTCGTGGTA GCGATCAAGC TATACCCAAA TTTCAAATGA ATTGTTACGC TAAAGAAGAT 
GCATTCAGTT ATCCGAGGAT GTATGAGGAA GCAAGTGGTA AGGAGGTAGA GAAGGTAGCA 
ACAGCTGTTC TTTCCACGAC AGCAAGAGCA AAAGCAAGGG CAAAGAAGAC AAAGAAGGAA 
AAGGGTCCAA ATGAGGAAGA AAAGAAAAAA GAGCATGAGG AAAAAGAAAA GGAAAGAGAG 
ACGAATAAAA AAGGCATAAA AGAAACAAAA GAAAACGACG AAGAATTTTA CAAAAATAAG 
TACTCTTCTA AACCTTATAA AGTCGACAAT ATGACCCGTA TACTACCACA ACAATCGAGA 
TATATTTCCT TTATTAAGGA CGACAGATTT GTTCCTGTAC GTAAATTCAA AGGAAATAAC 
GGTGTGGTGG TTTTAAGAGA TAGGGAACCC AAGGAACCTG TAGCACTAAT TGAAACTGTT 
AGGCAAATGA AAGATGTTAA CGCTCCACTT CCAACCCCAT TTAAGGTCGA TGATAATGTC 
GACTTCCCTA GTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012190.1
CDS	1..2838
Translation	MSLTTAAPLLALLRENQDSVKTYALESINNVVDQLWSEISNELPDIEALYDDDTFSDREMAALIASKVYYNLGEYESAVKYALAAKDRFDIDEKSQFVETIVSKSIEMYVQEASKQYTKDEQFYTKDIIDPKLTSIFERMIEKCLKASELKLALGIALEGYRLDIIESALKSKLDQDSTSENVKIINYLLTLAITTVTNSKFRSSILRKSFDFLMNMPNCDYLTLNKVVVNLNDAGLALQLFKKLKEENDEGLSAQIAFDLVSSASQQLLEILVTELTAQGYDPALLNILSGLPTCDYYNTFLLNNKNIDIGLLNKSKSSLDGKFSLFHTAVSVANGFMHAGTTDNSFIKANLPWLGKAQNWAKFTATASLGVIHKGNLLEGKKVMAPYLPGSRASSRFIKGGSLYGLGLIYAGFGRDTTDYLKNIIVENSGTSGDEDVDVLLHGASLGIGLAAMGSANIEVYEALKEVLYNDSATSGEAAALGMGLCMLGTGKPEAIHDMFTYSQETQHGNITRGLAVGLALINYGRQELADDLITKMLASDESLLRYGGAFTIALAYAGTGNNSAVKRLLHVAVSDSNDDVRRAAVIALGFVLLRDYTTVPRIVQLLSKSHNAHVRCGTAFALGIACAGKGLQSAIDVLDPLTKDPVDFVRQAAMIALSMILIQQTEKLNPQVADINKNFLSVITNKHQEGLAKFGACVAQGIMNAGGRNVTIQLENADTGTLDTKSVVGLVMFSQFWYWFPLAHFLSLSFTPTTVIGIRGSDQAIPKFQMNCYAKEDAFSYPRMYEEASGKEVEKVATAVLSTTARAKARAKKTKKEKGPNEEEKKKEHEEKEKERETNKKGIKETKENDEEFYKNKYSSKPYKVDNMTRILPQQSRYISFIKDDRFVPVRKFKGNNGVVVLRDREPKEPVALIETVRQMKDVNAPLPTPFKVDDNVDFPSA

Target ORF information:

RefSeq Version	NM_001179425.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C proteasome regulatory particle base subunit RPN2 (RPN2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179425.1

ORF Insert Sequence:

ATGTCTTTGA CGACTGCTGC TCCTCTTTTA GCATTGTTGA GAGAGAATCA AGATTCTGTT 
AAAACATATG CCCTAGAATC TATAAATAAT GTTGTGGACC AATTATGGTC TGAGATCTCG 
AATGAACTGC CGGATATTGA GGCATTGTAT GATGACGATA CCTTTTCAGA CCGTGAAATG 
GCTGCTTTGA TTGCATCCAA AGTTTATTAC AATCTTGGTG AATACGAATC GGCTGTGAAG 
TATGCACTTG CCGCAAAGGA TCGTTTTGAT ATAGACGAAA AGTCACAATT TGTTGAAACT 
ATAGTCTCAA AGAGTATTGA GATGTACGTC CAAGAGGCTT CCAAGCAATA CACTAAGGAC 
GAACAATTTT ACACCAAGGA TATTATTGAC CCAAAATTAA CCTCTATTTT TGAGCGAATG 
ATAGAAAAAT GTTTGAAAGC TTCAGAATTA AAATTAGCTT TAGGAATTGC CTTAGAAGGT 
TACAGATTGG ATATCATTGA AAGTGCTTTA AAGAGCAAAT TGGATCAGGA TTCCACTTCT 
GAAAACGTTA AAATTATTAA CTACTTATTG ACCTTAGCTA TAACTACTGT GACAAATTCC 
AAGTTCAGAT CCTCGATATT GAGAAAATCA TTTGATTTCC TAATGAATAT GCCTAACTGC 
GATTACTTAA CGCTCAATAA AGTAGTTGTA AATTTGAATG ACGCCGGATT GGCACTTCAG 
CTATTTAAAA AATTAAAGGA AGAGAACGAT GAAGGATTAT CTGCTCAAAT TGCATTTGAT 
TTGGTTTCGT CTGCGTCTCA GCAGTTGCTT GAGATTTTAG TAACCGAACT AACTGCCCAG 
GGTTACGATC CTGCCTTATT GAATATTCTA TCTGGGCTAC CGACTTGTGA TTATTACAAT 
ACCTTCCTTT TGAATAATAA AAACATTGAC ATCGGTCTTT TAAACAAGTC TAAATCATCT 
TTGGATGGGA AATTTTCTTT GTTCCACACC GCAGTCAGCG TTGCCAACGG TTTTATGCAC 
GCTGGTACTA CCGACAATTC ATTTATCAAG GCGAACTTGC CATGGTTAGG TAAGGCTCAA 
AATTGGGCTA AATTTACAGC TACAGCCTCT TTAGGTGTAA TTCATAAAGG CAATTTATTG 
GAGGGTAAAA AAGTCATGGC CCCTTACTTG CCAGGCAGTC GAGCTTCTTC TAGGTTTATC 
AAAGGTGGTT CATTGTATGG TTTGGGTTTA ATTTATGCTG GCTTTGGTCG TGATACAACT 
GACTATCTGA AAAACATAAT AGTTGAGAAT AGCGGAACTT CAGGCGATGA AGACGTAGAT 
GTATTGTTAC ATGGTGCTTC ACTGGGTATC GGTCTTGCTG CTATGGGTTC TGCTAATATT 
GAAGTTTATG AAGCCTTAAA GGAAGTTCTG TACAACGATT CTGCCACATC TGGAGAAGCA 
GCTGCATTAG GTATGGGTCT CTGTATGCTC GGAACAGGTA AGCCTGAAGC GATTCACGAT 
ATGTTTACAT ATTCCCAAGA AACGCAGCAT GGCAATATTA CTCGTGGTCT AGCCGTTGGT 
TTAGCACTGA TAAATTACGG CCGTCAAGAG TTAGCTGATG ATTTAATTAC TAAGATGTTA 
GCAAGTGATG AGTCCTTGTT ACGCTATGGA GGTGCATTCA CAATCGCGTT AGCGTATGCG 
GGTACGGGAA ATAATTCAGC TGTCAAGAGA TTACTGCATG TGGCAGTCTC GGATTCTAAC 
GATGATGTCA GAAGGGCAGC AGTCATCGCA TTAGGGTTTG TTCTCTTGCG TGATTATACC 
ACTGTACCAA GGATTGTCCA ACTTCTATCT AAATCGCATA ACGCTCATGT CAGATGTGGT 
ACCGCATTTG CTCTTGGGAT TGCTTGTGCC GGCAAGGGTT TACAATCAGC TATTGACGTT 
TTAGATCCGC TAACAAAGGA CCCAGTAGAT TTTGTTCGTC AGGCTGCCAT GATAGCTTTA 
TCCATGATTT TGATTCAACA AACAGAAAAA CTAAACCCTC AAGTTGCTGA TATTAACAAA 
AATTTTTTGA GCGTGATTAC AAACAAACAC CAAGAAGGTT TAGCCAAATT CGGTGCATGT 
GTGGCCCAAG GTATAATGAA TGCCGGTGGG CGTAATGTTA CAATTCAGCT GGAAAATGCG 
GATACAGGCA CATTGGATAC CAAGTCCGTA GTTGGTTTGG TCATGTTCTC ACAATTCTGG 
TACTGGTTCC CACTGGCTCA CTTTTTATCC CTTTCTTTTA CACCAACAAC TGTCATTGGT 
ATTCGTGGTA GCGATCAAGC TATACCCAAA TTTCAAATGA ATTGTTACGC TAAAGAAGAT 
GCATTCAGTT ATCCGAGGAT GTATGAGGAA GCAAGTGGTA AGGAGGTAGA GAAGGTAGCA 
ACAGCTGTTC TTTCCACGAC AGCAAGAGCA AAAGCAAGGG CAAAGAAGAC AAAGAAGGAA 
AAGGGTCCAA ATGAGGAAGA AAAGAAAAAA GAGCATGAGG AAAAAGAAAA GGAAAGAGAG 
ACGAATAAAA AAGGCATAAA AGAAACAAAA GAAAACGACG AAGAATTTTA CAAAAATAAG 
TACTCTTCTA AACCTTATAA AGTCGACAAT ATGACCCGTA TACTACCACA ACAATCGAGA 
TATATTTCCT TTATTAAGGA CGACAGATTT GTTCCTGTAC GTAAATTCAA AGGAAATAAC 
GGTGTGGTGG TTTTAAGAGA TAGGGAACCC AAGGAACCTG TAGCACTAAT TGAAACTGTT 
AGGCAAATGA AAGATGTTAA CGCTCCACTT CCAACCCCAT TTAAGGTCGA TGATAATGTC 
GACTTCCCTA GTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RPN2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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