SIM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SIM1
Entrez Gene ID	854683
Full Name	putative glucosidase SIM1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179471.2	NP_012143.2	putative glucosidase SIM1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	SUN4	NP_014333.1
Saccharomyces cerevisiae (baker's yeast)	SIM1	NP_012143.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ssd1 regulates translation of its target message primarily through UTRs and the SIM1 message through multiple potential points of interaction, permitting fine translational control in various contexts.
Title: Cell morphogenesis proteins are translationally controlled through UTRs by the Ndr/LATS target Ssd1.

SUN family proteins Sun4p, Uth1p and Sim1p are secreted from Saccharomyces cerevisiae and produced dependently on oxygen level.
Title: SUN family proteins Sun4p, Uth1p and Sim1p are secreted from Saccharomyces cerevisiae and produced dependently on oxygen level.

TORC2 has been shown previously to negatively regulate calcineurin in part by phosphorylating two related proteins, Slm1 and Slm2, which associate with the PM via plextrin homology domains
Title: Plasma membrane recruitment and activation of the AGC kinase Ypk1 is mediated by target of rapamycin complex 2 (TORC2) and its effector proteins Slm1 and Slm2.

The following SIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03719	NM_001179471.2 Latest version!	Saccharomyces cerevisiae S288C putative glucosidase SIM1 (SIM1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIM1 ( NM_001179471.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012143.2 Saccharomyces cerevisiae S288C putative glucosidase SIM1 (SIM1), partial mRNA.

Price & Availability↑

CloneID	OSi03719
Clone ID Related Accession (Same CDS sequence)	NM_001179471.2
Accession Version	NM_001179471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	putative glucosidase SIM1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). On Apr 26, 2011 this sequence version replaced NM_001179471.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAATTCT CAACTGCCGT TACTACGTTG ATTAGTTCTG GTGCCATCGT GTCTGCTTTA 
CCACACGTGG ATGTTCACCA AGAAGATGCC CACCAACATA AGAGGGCCGT TGCGTACAAA 
TACGTTTACG AAACTGTTGT TGTCGATTCT GATGGCCACA CTGTAACTCC TGCTGCTTCA 
GAAGTCGCTA CTGCTGCTAC CTCTGCTATC ATTACAACAT CTGTGTTGGC TCCAACCTCC 
TCCGCAGCCG CTGCGGATAG CTCCGCTTCC ATTGCTGTTT CATCTGCTGC CTTAGCCAAG 
AATGAGAAAA TCTCTGATGC CGCTGCATCT GCCACTGCCT CAACATCTCA AGGGGCATCC 
TCCTCCTCCT CCTCCTCCTC GGCAACTTCT ACCCTAGAAA GCAGCTCTGT TTCTTCATCT 
AGTGAAGAAG CTGCTCCAAC ATCTACTGTC GTGTCAACTT CTTCCGCAAC CCAATCTAGT 
GCTTCTTCTG CCACTAAATC TAGTACTTCT TCCACTTCAC CATCTACTTC TACTTCTACT 
TCCACTTCTT CTACTTCCTC TTCCTCTTCC TCCTCCTCCT CCTCTTCTTC TTCTTCTTCT 
GGCAGTGGTA GTATCTACGG TGATTTGGCC GACTTTTCAG GCCCAAGTGA GAAATTCCAA 
GACGGCACTA TTCCATGTGA CAAATTCCCA TCTGGTCAAG GTGTCATTTC TATTGACTGG 
ATTGGCGAGG GTGGATGGTC CGGTGTGGAA AACACCGACA CTTCCACTGG CGGTTCATGC 
AAGGAGGGGT CCTACTGTTC CTACTCCTGC CAACCAGGTA TGTCTAAGAC CCAATGGCCA 
TCCGATCAAC CATCTGACGG TAGATCTGTC GGGGGTTTGT TGTGTAAAAA TGGTTATTTG 
TACCGTTCTA ACACTGACGC GGATTACTTA TGTGAATGGG GTGTCGAGGC TGCCTATGTT 
GTTTCTAAAC TAAGCAAGGG TGTCGCCATT TGCAGAACCG ACTACCCGGG CACTGAAAAC 
ATGGTTATCC CAACCTATGT TGAAGGGGGT AGCTCTTTGC CATTGACCGT TGTTGACCAA 
GATACTTACT TTACTTGGGA AGGCAAAAAG ACATCTGCTC AATACTACGT TAATAACGCC 
GGCGTCTCAG TTGAAGATGG GTGTATCTGG GGTACTTCTG GATCTGGTAT TGGTAACTGG 
GCACCATTAA ACTTTGGTGC TGGCTCCACT GGTGGAGTGA CATACTTATC ATTGATTCCT 
AACCCAAACA ACAGCGACGC ATTGAACTAC AACGTCAAGA TAGTTGCTGC TGATGATTCA 
TCCAATGTCA TCGGTGAATG TGTTTACGAA AATGGTGAGT TCTCTGGCGG TGCTGACGGG 
TGTACCGTCT CTGTTACTTC CGGTAAAGCT CATTTCGTCT TATACAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012143.2
CDS	1..1431
Translation	MKFSTAVTTLISSGAIVSALPHVDVHQEDAHQHKRAVAYKYVYETVVVDSDGHTVTPAASEVATAATSAIITTSVLAPTSSAAAADSSASIAVSSAALAKNEKISDAAASATASTSQGASSSSSSSSATSTLESSSVSSSSEEAAPTSTVVSTSSATQSSASSATKSSTSSTSPSTSTSTSTSSTSSSSSSSSSSSSSSSGSGSIYGDLADFSGPSEKFQDGTIPCDKFPSGQGVISIDWIGEGGWSGVENTDTSTGGSCKEGSYCSYSCQPGMSKTQWPSDQPSDGRSVGGLLCKNGYLYRSNTDADYLCEWGVEAAYVVSKLSKGVAICRTDYPGTENMVIPTYVEGGSSLPLTVVDQDTYFTWEGKKTSAQYYVNNAGVSVEDGCIWGTSGSGIGNWAPLNFGAGSTGGVTYLSLIPNPNNSDALNYNVKIVAADDSSNVIGECVYENGEFSGGADGCTVSVTSGKAHFVLYN

Target ORF information:

RefSeq Version	NM_001179471.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C putative glucosidase SIM1 (SIM1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179471.2

ORF Insert Sequence:

ATGAAATTCT CAACTGCCGT TACTACGTTG ATTAGTTCTG GTGCCATCGT GTCTGCTTTA 
CCACACGTGG ATGTTCACCA AGAAGATGCC CACCAACATA AGAGGGCCGT TGCGTACAAA 
TACGTTTACG AAACTGTTGT TGTCGATTCT GATGGCCACA CTGTAACTCC TGCTGCTTCA 
GAAGTCGCTA CTGCTGCTAC CTCTGCTATC ATTACAACAT CTGTGTTGGC TCCAACCTCC 
TCCGCAGCCG CTGCGGATAG CTCCGCTTCC ATTGCTGTTT CATCTGCTGC CTTAGCCAAG 
AATGAGAAAA TCTCTGATGC CGCTGCATCT GCCACTGCCT CAACATCTCA AGGGGCATCC 
TCCTCCTCCT CCTCCTCCTC GGCAACTTCT ACCCTAGAAA GCAGCTCTGT TTCTTCATCT 
AGTGAAGAAG CTGCTCCAAC ATCTACTGTC GTGTCAACTT CTTCCGCAAC CCAATCTAGT 
GCTTCTTCTG CCACTAAATC TAGTACTTCT TCCACTTCAC CATCTACTTC TACTTCTACT 
TCCACTTCTT CTACTTCCTC TTCCTCTTCC TCCTCCTCCT CCTCTTCTTC TTCTTCTTCT 
GGCAGTGGTA GTATCTACGG TGATTTGGCC GACTTTTCAG GCCCAAGTGA GAAATTCCAA 
GACGGCACTA TTCCATGTGA CAAATTCCCA TCTGGTCAAG GTGTCATTTC TATTGACTGG 
ATTGGCGAGG GTGGATGGTC CGGTGTGGAA AACACCGACA CTTCCACTGG CGGTTCATGC 
AAGGAGGGGT CCTACTGTTC CTACTCCTGC CAACCAGGTA TGTCTAAGAC CCAATGGCCA 
TCCGATCAAC CATCTGACGG TAGATCTGTC GGGGGTTTGT TGTGTAAAAA TGGTTATTTG 
TACCGTTCTA ACACTGACGC GGATTACTTA TGTGAATGGG GTGTCGAGGC TGCCTATGTT 
GTTTCTAAAC TAAGCAAGGG TGTCGCCATT TGCAGAACCG ACTACCCGGG CACTGAAAAC 
ATGGTTATCC CAACCTATGT TGAAGGGGGT AGCTCTTTGC CATTGACCGT TGTTGACCAA 
GATACTTACT TTACTTGGGA AGGCAAAAAG ACATCTGCTC AATACTACGT TAATAACGCC 
GGCGTCTCAG TTGAAGATGG GTGTATCTGG GGTACTTCTG GATCTGGTAT TGGTAACTGG 
GCACCATTAA ACTTTGGTGC TGGCTCCACT GGTGGAGTGA CATACTTATC ATTGATTCCT 
AACCCAAACA ACAGCGACGC ATTGAACTAC AACGTCAAGA TAGTTGCTGC TGATGATTCA 
TCCAATGTCA TCGGTGAATG TGTTTACGAA AATGGTGAGT TCTCTGGCGG TGCTGACGGG 
TGTACCGTCT CTGTTACTTC CGGTAAAGCT CATTTCGTCT TATACAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SIM1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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