ORF » Species Summary » Saccharomyces cerevisiae S288C » TOP1 cDNA ORF clone

TOP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

  • Gene

  • Clones

  • gRNAs

The following TOP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03310 NM_001183260.1
Latest version!		 Saccharomyces cerevisiae S288C DNA topoisomerase 1 (TOP1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    TOP1 ( NM_001183260.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014637.1 Saccharomyces cerevisiae S288C DNA topoisomerase 1 (TOP1), partial mRNA.
    Price & Availability↑
    CloneID OSi03310
    Clone ID Related Accession (Same CDS sequence) NM_001183260.1
    Accession Version NM_001183260.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2310bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product DNA topoisomerase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTATTG CTGATGCTTC CAAAGTTAAT CATGAGTTGT CTTCTGATGA CGATGACGAT 
    GTGCCATTAT CTCAAACTTT AAAAAAAAGA AAGGTGGCGT CCATGAACTC TGCCTCTCTT 
    CAAGACGAAG CGGAACCTTA TGATAGTGAT GAGGCAATCT CTAAGATTTC CAAGAAAAAG 
    ACTAAGAAAA TAAAGACCGA ACCAGTGCAA TCGTCGTCAT TACCATCGCC TCCAGCAAAG 
    AAAAGCGCGA CATCAAAGCC TAAAAAAATC AAGAAAGAAG ATGGTGATGT AAAGGTAAAA 
    ACAACTAAAA AGGAAGAACA GGAGAACGAA AAAAAGAAAC GAGAAGAAGA AGAAGAGGAG 
    GACAAGAAAG CGAAGGAGGA GGAGGAAGAA TATAAATGGT GGGAAAAAGA AAACGAAGAT 
    GACACCATAA AATGGGTCAC ACTGAAGCAT AACGGTGTTA TATTCCCTCC ACCATACCAG 
    CCCTTACCAT CTCACATCAA ATTATATTAC GATGGGAAGC CAGTAGATTT ACCTCCGCAA 
    GCTGAAGAAG TAGCCGGGTT CTTTGCTGCC CTATTAGAGA GTGATCATGC CAAAAATCCT 
    GTTTTCCAAA AGAACTTCTT CAATGATTTC TTGCAAGTAC TGAAAGAAAG TGGTGGTCCC 
    CTCAATGGAA TTGAGATAAA GGAATTTTCT CGTTGCGATT TCACCAAAAT GTTTGATTAC 
    TTCCAGTTAC AAAAAGAACA GAAAAAGCAA CTGACTTCCC AAGAAAAGAA ACAGATTCGT 
    TTGGAAAGAG AAAAATTCGA GGAAGATTAT AAATTCTGTG AATTAGATGG CAGAAGGGAA 
    CAAGTAGGGA ATTTCAAGGT TGAACCTCCT GATCTATTTA GAGGTCGTGG CGCTCACCCA 
    AAAACAGGCA AATTGAAGAG AAGAGTGAAT CCTGAGGATA TCGTTTTAAA TCTAAGTAAA 
    GACGCACCCG TTCCGCCAGC CCCAGAAGGG CACAAGTGGG GTGAAATCAG ACACGACAAT 
    ACCGTTCAAT GGTTAGCCAT GTGGAGAGAG AATATTTTCA ACTCATTCAA ATACGTCAGA 
    TTGGCAGCGA ACTCTTCATT GAAGGGTCAA AGTGACTACA AGAAGTTTGA AAAGGCGAGA 
    CAATTGAAAT CCTATATCGA TGCCATCAGA AGGGATTACA CGAGAAATTT GAAAAGCAAA 
    GTTATGCTAG AGCGCCAAAA GGCCGTAGCC ATTTATTTGA TCGATGTATT CGCTTTAAGA 
    GCCGGTGGTG AAAAATCCGA AGATGAAGCC GATACTGTGG GTTGTTGTTC ATTGCGATAT 
    GAGCATGTTA CTTTGAAACC TCCGAATACT GTTATCTTTG ATTTCTTAGG TAAGGATTCT 
    ATTAGATTTT ATCAAGAGGT AGAAGTTGAC AAACAAGTTT TCAAAAATTT GACAATTTTT 
    AAAAGGCCGC CCAAACAGCC AGGACATCAA CTGTTTGATC GTCTAGATCC ATCTATACTG 
    AACAAATATC TACAAAACTA CATGCCGGGA TTGACTGCTA AAGTTTTCCG TACATATAAT 
    GCTTCCAAAA CAATGCAAGA TCAACTGGAT CTAATTCCAA ATAAAGGATC TGTCGCAGAG 
    AAAATATTGA AGTACAACGC AGCAAATAGA ACTGTAGCCA TCCTATGTAA CCATCAAAGG 
    ACTGTCACGA AGGGGCATGC ACAAACAGTG GAAAAGGCCA ATAATAGAAT ACAAGAGTTG 
    GAATGGCAAA AGATTCGTTG CAAGAGGGCC ATTTTACAAT TGGATAAGGA TCTTTTAAAG 
    AAAGAGCCAA AATATTTCGA AGAAATCGAC GATTTGACGA AAGAAGATGA AGCCACCATT 
    CACAAGAGAA TTATTGATAG AGAAATTGAA AAATATCAGC GAAAATTTGT TAGGGAGAAC 
    GATAAGAGGA AATTTGAAAA GGAAGAATTA TTGCCGGAAA GTCAATTGAA GGAATGGTTG 
    GAGAAAGTCG ACGAAAAGAA ACAAGAATTC GAAAAGGAAT TGAAAACCGG TGAAGTGGAA 
    CTGAAATCAA GTTGGAATTC AGTCGAAAAA ATAAAAGCAC AAGTAGAGAA ATTAGAACAG 
    CGTATCCAAA CTAGTTCCAT TCAGTTGAAA GATAAAGAGG AAAACTCCCA GGTTTCACTG 
    GGCACTTCCA AAATCAATTA TATAGACCCT AGACTTTCTG TGGTATTTTG CAAAAAGTAT 
    GATGTTCCGA TTGAAAAGAT TTTTACAAAA ACCCTAAGAG AAAAATTCAA ATGGGCCATA 
    GAATCGGTAG ATGAAAATTG GAGGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014637.1
    CDS1..2310
    Translation

    Target ORF information:

    RefSeq Version NM_001183260.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C DNA topoisomerase 1 (TOP1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001183260.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGACTATTG CTGATGCTTC CAAAGTTAAT CATGAGTTGT CTTCTGATGA CGATGACGAT 
    GTGCCATTAT CTCAAACTTT AAAAAAAAGA AAGGTGGCGT CCATGAACTC TGCCTCTCTT 
    CAAGACGAAG CGGAACCTTA TGATAGTGAT GAGGCAATCT CTAAGATTTC CAAGAAAAAG 
    ACTAAGAAAA TAAAGACCGA ACCAGTGCAA TCGTCGTCAT TACCATCGCC TCCAGCAAAG 
    AAAAGCGCGA CATCAAAGCC TAAAAAAATC AAGAAAGAAG ATGGTGATGT AAAGGTAAAA 
    ACAACTAAAA AGGAAGAACA GGAGAACGAA AAAAAGAAAC GAGAAGAAGA AGAAGAGGAG 
    GACAAGAAAG CGAAGGAGGA GGAGGAAGAA TATAAATGGT GGGAAAAAGA AAACGAAGAT 
    GACACCATAA AATGGGTCAC ACTGAAGCAT AACGGTGTTA TATTCCCTCC ACCATACCAG 
    CCCTTACCAT CTCACATCAA ATTATATTAC GATGGGAAGC CAGTAGATTT ACCTCCGCAA 
    GCTGAAGAAG TAGCCGGGTT CTTTGCTGCC CTATTAGAGA GTGATCATGC CAAAAATCCT 
    GTTTTCCAAA AGAACTTCTT CAATGATTTC TTGCAAGTAC TGAAAGAAAG TGGTGGTCCC 
    CTCAATGGAA TTGAGATAAA GGAATTTTCT CGTTGCGATT TCACCAAAAT GTTTGATTAC 
    TTCCAGTTAC AAAAAGAACA GAAAAAGCAA CTGACTTCCC AAGAAAAGAA ACAGATTCGT 
    TTGGAAAGAG AAAAATTCGA GGAAGATTAT AAATTCTGTG AATTAGATGG CAGAAGGGAA 
    CAAGTAGGGA ATTTCAAGGT TGAACCTCCT GATCTATTTA GAGGTCGTGG CGCTCACCCA 
    AAAACAGGCA AATTGAAGAG AAGAGTGAAT CCTGAGGATA TCGTTTTAAA TCTAAGTAAA 
    GACGCACCCG TTCCGCCAGC CCCAGAAGGG CACAAGTGGG GTGAAATCAG ACACGACAAT 
    ACCGTTCAAT GGTTAGCCAT GTGGAGAGAG AATATTTTCA ACTCATTCAA ATACGTCAGA 
    TTGGCAGCGA ACTCTTCATT GAAGGGTCAA AGTGACTACA AGAAGTTTGA AAAGGCGAGA 
    CAATTGAAAT CCTATATCGA TGCCATCAGA AGGGATTACA CGAGAAATTT GAAAAGCAAA 
    GTTATGCTAG AGCGCCAAAA GGCCGTAGCC ATTTATTTGA TCGATGTATT CGCTTTAAGA 
    GCCGGTGGTG AAAAATCCGA AGATGAAGCC GATACTGTGG GTTGTTGTTC ATTGCGATAT 
    GAGCATGTTA CTTTGAAACC TCCGAATACT GTTATCTTTG ATTTCTTAGG TAAGGATTCT 
    ATTAGATTTT ATCAAGAGGT AGAAGTTGAC AAACAAGTTT TCAAAAATTT GACAATTTTT 
    AAAAGGCCGC CCAAACAGCC AGGACATCAA CTGTTTGATC GTCTAGATCC ATCTATACTG 
    AACAAATATC TACAAAACTA CATGCCGGGA TTGACTGCTA AAGTTTTCCG TACATATAAT 
    GCTTCCAAAA CAATGCAAGA TCAACTGGAT CTAATTCCAA ATAAAGGATC TGTCGCAGAG 
    AAAATATTGA AGTACAACGC AGCAAATAGA ACTGTAGCCA TCCTATGTAA CCATCAAAGG 
    ACTGTCACGA AGGGGCATGC ACAAACAGTG GAAAAGGCCA ATAATAGAAT ACAAGAGTTG 
    GAATGGCAAA AGATTCGTTG CAAGAGGGCC ATTTTACAAT TGGATAAGGA TCTTTTAAAG 
    AAAGAGCCAA AATATTTCGA AGAAATCGAC GATTTGACGA AAGAAGATGA AGCCACCATT 
    CACAAGAGAA TTATTGATAG AGAAATTGAA AAATATCAGC GAAAATTTGT TAGGGAGAAC 
    GATAAGAGGA AATTTGAAAA GGAAGAATTA TTGCCGGAAA GTCAATTGAA GGAATGGTTG 
    GAGAAAGTCG ACGAAAAGAA ACAAGAATTC GAAAAGGAAT TGAAAACCGG TGAAGTGGAA 
    CTGAAATCAA GTTGGAATTC AGTCGAAAAA ATAAAAGCAC AAGTAGAGAA ATTAGAACAG 
    CGTATCCAAA CTAGTTCCAT TCAGTTGAAA GATAAAGAGG AAAACTCCCA GGTTTCACTG 
    GGCACTTCCA AAATCAATTA TATAGACCCT AGACTTTCTG TGGTATTTTG CAAAAAGTAT 
    GATGTTCCGA TTGAAAAGAT TTTTACAAAA ACCCTAAGAG AAAAATTCAA ATGGGCCATA 
    GAATCGGTAG ATGAAAATTG GAGGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
    Nature387(6632 Suppl)98-102(1997 May)
    Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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