ORF » Species Summary » Saccharomyces cerevisiae S288C » THI20 cDNA ORF clone

THI20 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following THI20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THI20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03264 NM_001183310.1
Latest version!		 Saccharomyces cerevisiae S288C trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase (THI20), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    THI20 ( NM_001183310.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014586.1 Saccharomyces cerevisiae S288C trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase (THI20), partial mRNA.
    Price & Availability↑
    CloneID OSi03264
    Clone ID Related Accession (Same CDS sequence) NM_001183310.1
    Accession Version NM_001183310.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCTATT CTACAGTTAG CATCAACACG CCTCCACCAT ATCTTACTTT AGCCTGCAAC 
    GAAAAGTTGC CAACAGTTTT ATCCATTGCT GGAACAGATC CAAGTGGTGG AGCCGGTATC 
    GAAGCTGATG TCAAAACTAT CACAGCACAC AGATGTTATG CCATGACATG TATCACTGCT 
    TTAAATGCTC AAACTCCAGT TAAAGTCTAC AGCATAAATA ACACACCAAA AGAAGTGGTT 
    TTCCAAACTT TGGAATCCAA TTTAAAGGAT ATGAAATGTA ATGTTATCAA AACCGGTATG 
    CTTACCGCAG CTGCTATCGA AGTCTTGCAC GAAAAACTGC TGCAACTCGG CGAAAATAGA 
    CCTAAGTTGG TTGTTGATCC AGTTCTTGTC GCTACTTCTG GTTCTTCTTT AGCTGGAAAG 
    GATATAGTTA GTTTGATTAC GGAGAAGGTT GCACCTTTTG CTGATATTTT GACTCCTAAC 
    ATCCCAGAGT GTTACAAATT GTTAGGTGAG GAAAGAAAGG TTAACGGATT ACAGGATATT 
    TTCCAAATCG CAAAAGACCT TGCTAAAATA ACCAAGTGTT CGAATATACT AGTGAAGGGT 
    GGCCATATTC CATGGAATGA TGAAAAGGAA AAGTACATTA CCGACGTTCT TTTCTTGGGT 
    GCTGAACAAA AATTCATTAT CTTTAAAGGC AATTTCGTTA ACACCACGCA TACACATGGT 
    ACTGGGTGCA CACTAGCTTC TGCCATTGCA TCAAACTTGG CTCGTGGTTA TTCTCTTCCT 
    CAGTCTGTTT ATGGTGGTAT TGAATACGTC CAAAATGCCG TGGCAATTGG TTGTGATGTC 
    ACAAAAGAAA CTGTCAAGGA TAATGGACCA ATTAATCATG TTTACGCCGT GGAAATTCCA 
    TTGGAGAAAA TGCTCAGTGA TGAATGTTTT ACTGCATCGG ATGTTATACC TAAAAAGCCG 
    CTCAAAAGTG CTGCTGACAA AATCCCAGGC GGTAATTTCT ATGAATACCT GATCAATCAC 
    CCTAAAGTTA AGCCACACTG GGATTCCTAT ATTAACCATG AGTTTGTTAA AAAGGTTGCG 
    GACGGTACAT TAGAACGTAA GAAGTTTCAG TTCTTTATTG AACAAGATTA TGCATACTTG 
    GTTGATTATG CTAGAGTTCA TTGTATCGCG GGTAGTAAAG CTCCATGCCT AGAAGATATG 
    GAAAAGGAGC TAGTCATTGT TGGAGGTGTT CGTACTGAAA TGGGTCAACA TGAAAAGAGA 
    TTAAAAGAAG TATTTGGTGT TAAAGATCCT GATTACTTTC AAAAGATTAA GAGAGGACCT 
    GCATTAAGAG CTTACTCTCG TTACTTTAAC GATGTTTCCA GAAGAGGTAA CTGGCAAGAA 
    TTGGTTGCAT CCCTTACTCC TTGTTTGATG GGTTATGGTG AAGCTTTAAC TAAAATGAAG 
    GGTAAAGTCA CCGCACCTGA AGGGTCCGTT TACCACGAAT GGTGTGAAAC TTACGCATCT 
    TCATGGTATC GTGAAGCCAT GGATGAAGGT GAAAAACTTT TGAACCACAT CTTAGAAACA 
    TACCCTCCAG AGCAACTTGA CACTTTAGTA ACAATCTACG CTGAAGTTTG TGAATTAGAA 
    ACAAATTTTT GGACCGCTGC GCTAGAATAC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_014586.1
    CDS1..1656
    Translation

    Target ORF information:

    RefSeq Version NM_001183310.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase (THI20), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001183310.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGACCTATT CTACAGTTAG CATCAACACG CCTCCACCAT ATCTTACTTT AGCCTGCAAC 
    GAAAAGTTGC CAACAGTTTT ATCCATTGCT GGAACAGATC CAAGTGGTGG AGCCGGTATC 
    GAAGCTGATG TCAAAACTAT CACAGCACAC AGATGTTATG CCATGACATG TATCACTGCT 
    TTAAATGCTC AAACTCCAGT TAAAGTCTAC AGCATAAATA ACACACCAAA AGAAGTGGTT 
    TTCCAAACTT TGGAATCCAA TTTAAAGGAT ATGAAATGTA ATGTTATCAA AACCGGTATG 
    CTTACCGCAG CTGCTATCGA AGTCTTGCAC GAAAAACTGC TGCAACTCGG CGAAAATAGA 
    CCTAAGTTGG TTGTTGATCC AGTTCTTGTC GCTACTTCTG GTTCTTCTTT AGCTGGAAAG 
    GATATAGTTA GTTTGATTAC GGAGAAGGTT GCACCTTTTG CTGATATTTT GACTCCTAAC 
    ATCCCAGAGT GTTACAAATT GTTAGGTGAG GAAAGAAAGG TTAACGGATT ACAGGATATT 
    TTCCAAATCG CAAAAGACCT TGCTAAAATA ACCAAGTGTT CGAATATACT AGTGAAGGGT 
    GGCCATATTC CATGGAATGA TGAAAAGGAA AAGTACATTA CCGACGTTCT TTTCTTGGGT 
    GCTGAACAAA AATTCATTAT CTTTAAAGGC AATTTCGTTA ACACCACGCA TACACATGGT 
    ACTGGGTGCA CACTAGCTTC TGCCATTGCA TCAAACTTGG CTCGTGGTTA TTCTCTTCCT 
    CAGTCTGTTT ATGGTGGTAT TGAATACGTC CAAAATGCCG TGGCAATTGG TTGTGATGTC 
    ACAAAAGAAA CTGTCAAGGA TAATGGACCA ATTAATCATG TTTACGCCGT GGAAATTCCA 
    TTGGAGAAAA TGCTCAGTGA TGAATGTTTT ACTGCATCGG ATGTTATACC TAAAAAGCCG 
    CTCAAAAGTG CTGCTGACAA AATCCCAGGC GGTAATTTCT ATGAATACCT GATCAATCAC 
    CCTAAAGTTA AGCCACACTG GGATTCCTAT ATTAACCATG AGTTTGTTAA AAAGGTTGCG 
    GACGGTACAT TAGAACGTAA GAAGTTTCAG TTCTTTATTG AACAAGATTA TGCATACTTG 
    GTTGATTATG CTAGAGTTCA TTGTATCGCG GGTAGTAAAG CTCCATGCCT AGAAGATATG 
    GAAAAGGAGC TAGTCATTGT TGGAGGTGTT CGTACTGAAA TGGGTCAACA TGAAAAGAGA 
    TTAAAAGAAG TATTTGGTGT TAAAGATCCT GATTACTTTC AAAAGATTAA GAGAGGACCT 
    GCATTAAGAG CTTACTCTCG TTACTTTAAC GATGTTTCCA GAAGAGGTAA CTGGCAAGAA 
    TTGGTTGCAT CCCTTACTCC TTGTTTGATG GGTTATGGTG AAGCTTTAAC TAAAATGAAG 
    GGTAAAGTCA CCGCACCTGA AGGGTCCGTT TACCACGAAT GGTGTGAAAC TTACGCATCT 
    TCATGGTATC GTGAAGCCAT GGATGAAGGT GAAAAACTTT TGAACCACAT CTTAGAAACA 
    TACCCTCCAG AGCAACTTGA CACTTTAGTA ACAATCTACG CTGAAGTTTG TGAATTAGAA 
    ACAAATTTTT GGACCGCTGC GCTAGAATAC GAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
    Nature387(6632 Suppl)98-102(1997 May)
    Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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