ORF » Species Summary » Saccharomyces cerevisiae S288C » PCK1 cDNA ORF clone

PCK1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following PCK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi03149 NM_001179887.3
Latest version!		 Saccharomyces cerevisiae S288C phosphoenolpyruvate carboxykinase PCK1 (PCK1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PCK1 ( NM_001179887.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013023.3 Saccharomyces cerevisiae S288C phosphoenolpyruvate carboxykinase PCK1 (PCK1), partial mRNA.
    Price & Availability↑
    CloneID OSi03149
    Clone ID Related Accession (Same CDS sequence) NM_001179887.3
    Accession Version NM_001179887.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product phosphoenolpyruvate carboxykinase PCK1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). On Jul 30, 2012 this sequence version replaced NM_001179887.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCCCCTT CTAAAATGAA TGCTACAGTA GGATCTACTT CCGAAGTTGA ACAAAAAATC 
    AGACAAGAAT TGGCTCTTAG TGACGAAGTC ACCACCATCA GACGCAATGC TCCAGCTGCC 
    GTTTTGTATG AAGATGGTCT AAAAGAAAAT AAAACTGTCA TTTCATCAAG CGGTGCATTG 
    ATCGCTTATT CCGGTGTTAA AACCGGAAGA TCTCCAAAGG ACAAACGTAT TGTTGAAGAA 
    CCTACCTCGA AAGACGAAAT TTGGTGGGGT CCGGTCAATA AACCATGTTC TGAAAGAACA 
    TGGTCTATCA ACCGTGAAAG AGCTGCAGAT TACTTGAGAA CAAGAGACCA CATTTATATT 
    GTCGATGCAT TTGCAGGATG GGATCCAAAA TACAGAATCA AAGTCCGCGT TGTTTGTGCC 
    AGGGCTTACC ACGCTTTATT CATGACAAAT ATGCTTATTA GACCTACAGA AGAAGAATTA 
    GCCCATTTTG GAGAACCTGA TTTTACTGTC TGGAACGCTG GTCAGTTCCC AGCCAATTTA 
    CACACCCAGG ATATGTCTTC AAAGAGTACT ATAGAAATTA ACTTCAAAGC AATGGAAATG 
    ATCATTTTAG GTACCGAATA CGCCGGTGAA ATGAAAAAAG GTATTTTCAC AGTTATGTTT 
    TACTTGATGC CTGTGCACCA TAACGTTTTA ACTTTGCACT CTTCCGCCAA CCAGGGTATT 
    CAAAACGGTG ACGTTACTTT ATTCTTTGGC CTAAGTGGTA CCGGGAAAAC CACTTTATCC 
    GCAGACCCAC ATAGATTGTT GATCGGCGAT GATGAACATT GTTGGTCCGA CCATGGTGTC 
    TTCAATATCG AAGGTGGTTG TTACGCCAAG TGTATTAATT TATCTGCCGA AAAGGAGCCT 
    GAAATTTTCG ACGCTATCAA GTTTGGTTCT GTATTAGAAA ACGTTATCTA TGACGAGAAG 
    TCGCATGTAG TCGACTATGA CGACTCTTCT ATTACTGAAA ATACTAGATG TGCCTACCCA 
    ATTGACTACA TTCCAAGTGC CAAGATTCCA TGTTTGGCGG ACTCTCATCC AAAGAACATT 
    ATCCTGCTAA CTTGTGATGC TTCGGGTGTT TTACCACCAG TATCTAAATT GACTCCTGAA 
    CAAGTCATGT ACCATTTCAT CTCTGGTTAC ACTTCTAAAA TGGCTGGTAC TGAGCAAGGT 
    GTCACTGAAC CTGAACCAAC ATTTTCATCT TGTTTCGGAC AACCCTTCCT AGCCTTGCAC 
    CCTATTAGAT ACGCAACCAT GTTAGCTACA AAGATGTCTC AACATAAAGC TAATGCGTAC 
    TTAATCAACA CCGGCTGGAC TGGTTCTTCC TACGTATCTG GTGGTAAACG TTGCCCATTG 
    AAGTACACAA GGGCCATTCT GGATTCTATT CATGATGGTT CGTTAGCCAA TGAAACGTAC 
    GAAACTTTAC CGATTTTCAA TCTTCAAGTA CCTACCAAGG TTAACGGTGT TCCAGCTGAG 
    CTTTTGAATC CTGCTAAAAA CTGGTCTCAA GGTGAATCCA AATACAGAGG TGCAGTTACC 
    AACTTGGCCA ACTTGTTTGT TCAAAATTTC AAGATTTATC AAGACAGAGC CACACCAGAT 
    GTATTAGCCG CTGGTCCTCA ATTCGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_013023.3
    CDS1..1650
    Translation

    Target ORF information:

    RefSeq Version NM_001179887.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C phosphoenolpyruvate carboxykinase PCK1 (PCK1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001179887.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCCCCTT CTAAAATGAA TGCTACAGTA GGATCTACTT CCGAAGTTGA ACAAAAAATC 
    AGACAAGAAT TGGCTCTTAG TGACGAAGTC ACCACCATCA GACGCAATGC TCCAGCTGCC 
    GTTTTGTATG AAGATGGTCT AAAAGAAAAT AAAACTGTCA TTTCATCAAG CGGTGCATTG 
    ATCGCTTATT CCGGTGTTAA AACCGGAAGA TCTCCAAAGG ACAAACGTAT TGTTGAAGAA 
    CCTACCTCGA AAGACGAAAT TTGGTGGGGT CCGGTCAATA AACCATGTTC TGAAAGAACA 
    TGGTCTATCA ACCGTGAAAG AGCTGCAGAT TACTTGAGAA CAAGAGACCA CATTTATATT 
    GTCGATGCAT TTGCAGGATG GGATCCAAAA TACAGAATCA AAGTCCGCGT TGTTTGTGCC 
    AGGGCTTACC ACGCTTTATT CATGACAAAT ATGCTTATTA GACCTACAGA AGAAGAATTA 
    GCCCATTTTG GAGAACCTGA TTTTACTGTC TGGAACGCTG GTCAGTTCCC AGCCAATTTA 
    CACACCCAGG ATATGTCTTC AAAGAGTACT ATAGAAATTA ACTTCAAAGC AATGGAAATG 
    ATCATTTTAG GTACCGAATA CGCCGGTGAA ATGAAAAAAG GTATTTTCAC AGTTATGTTT 
    TACTTGATGC CTGTGCACCA TAACGTTTTA ACTTTGCACT CTTCCGCCAA CCAGGGTATT 
    CAAAACGGTG ACGTTACTTT ATTCTTTGGC CTAAGTGGTA CCGGGAAAAC CACTTTATCC 
    GCAGACCCAC ATAGATTGTT GATCGGCGAT GATGAACATT GTTGGTCCGA CCATGGTGTC 
    TTCAATATCG AAGGTGGTTG TTACGCCAAG TGTATTAATT TATCTGCCGA AAAGGAGCCT 
    GAAATTTTCG ACGCTATCAA GTTTGGTTCT GTATTAGAAA ACGTTATCTA TGACGAGAAG 
    TCGCATGTAG TCGACTATGA CGACTCTTCT ATTACTGAAA ATACTAGATG TGCCTACCCA 
    ATTGACTACA TTCCAAGTGC CAAGATTCCA TGTTTGGCGG ACTCTCATCC AAAGAACATT 
    ATCCTGCTAA CTTGTGATGC TTCGGGTGTT TTACCACCAG TATCTAAATT GACTCCTGAA 
    CAAGTCATGT ACCATTTCAT CTCTGGTTAC ACTTCTAAAA TGGCTGGTAC TGAGCAAGGT 
    GTCACTGAAC CTGAACCAAC ATTTTCATCT TGTTTCGGAC AACCCTTCCT AGCCTTGCAC 
    CCTATTAGAT ACGCAACCAT GTTAGCTACA AAGATGTCTC AACATAAAGC TAATGCGTAC 
    TTAATCAACA CCGGCTGGAC TGGTTCTTCC TACGTATCTG GTGGTAAACG TTGCCCATTG 
    AAGTACACAA GGGCCATTCT GGATTCTATT CATGATGGTT CGTTAGCCAA TGAAACGTAC 
    GAAACTTTAC CGATTTTCAA TCTTCAAGTA CCTACCAAGG TTAACGGTGT TCCAGCTGAG 
    CTTTTGAATC CTGCTAAAAA CTGGTCTCAA GGTGAATCCA AATACAGAGG TGCAGTTACC 
    AACTTGGCCA ACTTGTTTGT TCAAAATTTC AAGATTTATC AAGACAGAGC CACACCAGAT 
    GTATTAGCCG CTGGTCCTCA ATTCGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete DNA sequence of yeast chromosome XI.
    Nature369(6479)371-8(1994 Jun)
    Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW


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