TPK3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TPK3
Entrez Gene ID	853688
Full Name	cAMP-dependent protein kinase catalytic subunit TPK3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179732.1	NP_012755.1	cAMP-dependent protein kinase catalytic subunit TPK3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	prkacaa	NP_001076309.1
Kluyveromyces lactis	KLLA0B07205g	XP_451851.1
Pan troglodytes (chimpanzee)	PRKACG	XP_528314.3
Eremothecium gossypii	AGOS_AEL115C	NP_984746.2
Homo sapiens (human)	PRKACG	NP_002723.2
Saccharomyces cerevisiae (baker's yeast)	TPK3	NP_012755.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome XI.
Dujon B, Alexandraki D, Andr? B, Ansorge W, Baladron V, Ballesta JP, Banrevi A, Bolle PA, Bolotin-Fukuhara M, Bossier P, Bou G, Boyer J, Bultrago MJ, Cheret G, Colleaux L, Dalgnan-Fornler B, del Rey F, Dlon C, Domdey H, D?sterhoft A, D?sterhus S, Entlan KD, Erfle H, Esteban PF, Feldmann H, Fernandes L, Robo GM, Fritz C, Fukuhara H, Gabel C, Gaillon L, Carcia-Cantalejo JM, Garcia-Ramirez JJ, Gent NE, Ghazvini M, Goffeau A, Gonzal?z A, Grothues D, Guerreiro P, Hegemann J, Hewitt N, Hilger F, Hollenberg CP, Horaitis O, Indge KJ, Jacquier A, James CM, Jauniaux C, Jimenez A, Keuchel H, Kirchrath L, Kleine K, K?tter P, Legrain P, Liebl S, Louis EJ, Maia e Silva A, Marck C, Monnier AL, M?stl D, M?ller S, Obermaier B, Oliver SG, Pallier C, Pascolo S, Pfeiffer F, Philippsen P, Planta RJ, Pohl FM, Pohl TM, P?hlmann R, Portetelle D, Purnelle B, Puzos V, Ramezani Rad M, Rasmussen SW, Remacha M, Revuelta JL, Richard GF, Rieger M, Rodrigues-Pousada C, Rose M, Rupp T, Santos MA, Schwager C, Sensen C, Skala J, Soares H, Sor F, Stegemann J, Tettelin H, Thierry A, Tzermia M, Urrestarazu LA, van Dyck L, Van Vliet-Reedijk JC, Valens M, Vandenbo M, Vilela C, Vissers S, von Wettstein D, Voss H, Wiemann S, Xu G, Zimmermann J, Haasemann M, Becker I, Mewes HW
Nature369(6479)371-8(1994 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Rgt1 and tpk1/2/3 (cAMP-dependent protein kinases) participate in the control of SUC2 expression.
Title: The repressor Rgt1 and the cAMP-dependent protein kinases control the expression of the SUC2 gene in Saccharomyces cerevisiae.

Tpk3 is poorly phosphorylated on its activation loop site due to its weak interaction with PKB (protein kinase B)-activating kinase homologue Pkh1.
Title: The activation loop of PKA catalytic isoforms is differentially phosphorylated by Pkh protein kinases in Saccharomyces cerevisiae.

Trehalase activation is associated with phosphorylation of typical PKA sites.
Title: In vivo phosphorylation of Ser21 and Ser83 during nutrient-induced activation of the yeast protein kinase A (PKA) target trehalase.

Tpk2 and Tpk3 are associated with translation initiation factors Pab1 and Rps3 in exponentially growing cells.
Title: PKA isoforms coordinate mRNA fate during nutrient starvation.

Data show that the Ras2p localization is regulated by PKA, and that PKA down-regulates Ras2p activity and the protein association between Cdc25p and Ras2-GTP, which is due to a reduced Ras2-GEF Cdc25p activity.
Title: The membrane localization of Ras2p and the association between Cdc25p and Ras2-GTP are regulated by protein kinase A (PKA) in the yeast Saccharomyces cerevisiae.

The following TPK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02905	NM_001179732.1 Latest version!	Saccharomyces cerevisiae S288C cAMP-dependent protein kinase catalytic subunit TPK3 (TPK3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPK3 ( NM_001179732.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012755.1 Saccharomyces cerevisiae S288C cAMP-dependent protein kinase catalytic subunit TPK3 (TPK3), partial mRNA.

Price & Availability↑

CloneID	OSi02905
Clone ID Related Accession (Same CDS sequence)	NM_001179732.1
Accession Version	NM_001179732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	cAMP-dependent protein kinase catalytic subunit TPK3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001143). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTATGTTG ATCCGATGAA CAACAATGAA ATCAGGAAAT TAAGCATTAC TGCCAAGACA 
GAAACAACTC CAGATAACGT TGGACAAGAC ATTCCTGTAA ACGCACATTC GGTGCATGAG 
GAATGTTCTT CCAACACACC CGTGGAGATA AATGGAAGAA ACAGCGGAAA GTTGAAAGAA 
GAAGCGTCTG CAGGTATTTG TTTGGTTAAA AAACCAATGC TACAATATAG AGATACCTCA 
GGAAAGTATT CCCTAAGTGA CTTTCAGATT TTAAGAACTT TGGGAACTGG CTCATTTGGG 
AGAGTTCACC TAATTCGTTC CAATCACAAT GGGAGGTTTT ACGCTTTGAA GACATTGAAA 
AAGCACACTA TAGTGAAGCT GAAGCAGGTT GAACACACCA ATGACGAACG CCGAATGCTT 
TCAATTGTTT CACATCCATT CATCATTCGA ATGTGGGGAA CGTTCCAAGA TTCTCAGCAA 
GTTTTCATGG TAATGGACTA CATTGAAGGT GGTGAATTAT TTTCTTTACT ACGTAAATCT 
CAAAGATTTC CCAACCCAGT AGCCAAATTT TATGCCGCAG AGGTATGCTT AGCGTTGGAA 
TATTTGCACA GTAAGGATAT AATATATAGA GACTTGAAAC CAGAAAATAT CCTTCTAGAT 
AAAAACGGCC ATATCAAGAT AACCGACTTT GGCTTCGCAA AATACGTTCC CGATGTCACA 
TACACATTAT GTGGCACACC AGATTACATA GCGCCGGAAG TGGTCAGTAC AAAACCGTAT 
AATAAATCAG TGGATTGGTG GAGTTTTGGT GTGCTAATCT ATGAAATGCT TGCCGGATAC 
ACTCCATTTT ACAATTCGAA CACCATGAAA ACTTACGAAA ATATACTGAA CGCCGAATTG 
AAGTTCCCAC CATTTTTCCA TCCAGACGCG CAGGACTTAT TGAAGAAGCT AATTACCAGA 
GACTTAAGTG AAAGGTTGGG TAACTTACAA AATGGAAGTG AAGATGTCAA GAACCATCCG 
TGGTTTAACG AAGTGATATG GGAGAAATTG TTAGCAAGAT ACATAGAAAC GCCGTACGAA 
CCACCAATCC AACAGGGCCA AGGTGACACT TCTCAATTTG ACAGATACCC TGAAGAGGAA 
TTCAACTATG GAATTCAAGG GGAGGATCCA TATATGGATT TAATGAAAGA ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012755.1
CDS	1..1197
Translation	MYVDPMNNNEIRKLSITAKTETTPDNVGQDIPVNAHSVHEECSSNTPVEINGRNSGKLKEEASAGICLVKKPMLQYRDTSGKYSLSDFQILRTLGTGSFGRVHLIRSNHNGRFYALKTLKKHTIVKLKQVEHTNDERRMLSIVSHPFIIRMWGTFQDSQQVFMVMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFGFAKYVPDVTYTLCGTPDYIAPEVVSTKPYNKSVDWWSFGVLIYEMLAGYTPFYNSNTMKTYENILNAELKFPPFFHPDAQDLLKKLITRDLSERLGNLQNGSEDVKNHPWFNEVIWEKLLARYIETPYEPPIQQGQGDTSQFDRYPEEEFNYGIQGEDPYMDLMKEF

Target ORF information:

RefSeq Version	NM_001179732.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C cAMP-dependent protein kinase catalytic subunit TPK3 (TPK3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179732.1

ORF Insert Sequence:

ATGTATGTTG ATCCGATGAA CAACAATGAA ATCAGGAAAT TAAGCATTAC TGCCAAGACA 
GAAACAACTC CAGATAACGT TGGACAAGAC ATTCCTGTAA ACGCACATTC GGTGCATGAG 
GAATGTTCTT CCAACACACC CGTGGAGATA AATGGAAGAA ACAGCGGAAA GTTGAAAGAA 
GAAGCGTCTG CAGGTATTTG TTTGGTTAAA AAACCAATGC TACAATATAG AGATACCTCA 
GGAAAGTATT CCCTAAGTGA CTTTCAGATT TTAAGAACTT TGGGAACTGG CTCATTTGGG 
AGAGTTCACC TAATTCGTTC CAATCACAAT GGGAGGTTTT ACGCTTTGAA GACATTGAAA 
AAGCACACTA TAGTGAAGCT GAAGCAGGTT GAACACACCA ATGACGAACG CCGAATGCTT 
TCAATTGTTT CACATCCATT CATCATTCGA ATGTGGGGAA CGTTCCAAGA TTCTCAGCAA 
GTTTTCATGG TAATGGACTA CATTGAAGGT GGTGAATTAT TTTCTTTACT ACGTAAATCT 
CAAAGATTTC CCAACCCAGT AGCCAAATTT TATGCCGCAG AGGTATGCTT AGCGTTGGAA 
TATTTGCACA GTAAGGATAT AATATATAGA GACTTGAAAC CAGAAAATAT CCTTCTAGAT 
AAAAACGGCC ATATCAAGAT AACCGACTTT GGCTTCGCAA AATACGTTCC CGATGTCACA 
TACACATTAT GTGGCACACC AGATTACATA GCGCCGGAAG TGGTCAGTAC AAAACCGTAT 
AATAAATCAG TGGATTGGTG GAGTTTTGGT GTGCTAATCT ATGAAATGCT TGCCGGATAC 
ACTCCATTTT ACAATTCGAA CACCATGAAA ACTTACGAAA ATATACTGAA CGCCGAATTG 
AAGTTCCCAC CATTTTTCCA TCCAGACGCG CAGGACTTAT TGAAGAAGCT AATTACCAGA 
GACTTAAGTG AAAGGTTGGG TAACTTACAA AATGGAAGTG AAGATGTCAA GAACCATCCG 
TGGTTTAACG AAGTGATATG GGAGAAATTG TTAGCAAGAT ACATAGAAAC GCCGTACGAA 
CCACCAATCC AACAGGGCCA AGGTGACACT TCTCAATTTG ACAGATACCC TGAAGAGGAA 
TTCAACTATG GAATTCAAGG GGAGGATCCA TATATGGATT TAATGAAAGA ATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome XI.
Nature369(6479)371-8(1994 Jun)
Dujon B,Alexandraki D,Andr? B,Ansorge W,Baladron V,Ballesta JP,Banrevi A,Bolle PA,Bolotin-Fukuhara M,Bossier P,Bou G,Boyer J,Bultrago MJ,Cheret G,Colleaux L,Dalgnan-Fornler B,del Rey F,Dlon C,Domdey H,D?sterhoft A,D?sterhus S,Entlan KD,Erfle H,Esteban PF,Feldmann H,Fernandes L,Robo GM,Fritz C,Fukuhara H,Gabel C,Gaillon L,Carcia-Cantalejo JM,Garcia-Ramirez JJ,Gent NE,Ghazvini M,Goffeau A,Gonzal?z A,Grothues D,Guerreiro P,Hegemann J,Hewitt N,Hilger F,Hollenberg CP,Horaitis O,Indge KJ,Jacquier A,James CM,Jauniaux C,Jimenez A,Keuchel H,Kirchrath L,Kleine K,K?tter P,Legrain P,Liebl S,Louis EJ,Maia e Silva A,Marck C,Monnier AL,M?stl D,M?ller S,Obermaier B,Oliver SG,Pallier C,Pascolo S,Pfeiffer F,Philippsen P,Planta RJ,Pohl FM,Pohl TM,P?hlmann R,Portetelle D,Purnelle B,Puzos V,Ramezani Rad M,Rasmussen SW,Remacha M,Revuelta JL,Richard GF,Rieger M,Rodrigues-Pousada C,Rose M,Rupp T,Santos MA,Schwager C,Sensen C,Skala J,Soares H,Sor F,Stegemann J,Tettelin H,Thierry A,Tzermia M,Urrestarazu LA,van Dyck L,Van Vliet-Reedijk JC,Valens M,Vandenbo M,Vilela C,Vissers S,von Wettstein D,Voss H,Wiemann S,Xu G,Zimmermann J,Haasemann M,Becker I,Mewes HW

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TPK3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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