ORF » Species Summary » Saccharomyces cerevisiae S288C » MDE1 cDNA ORF clone

MDE1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following MDE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02723 NM_001181682.3
Latest version!		 Saccharomyces cerevisiae S288C methylthioribulose 1-phosphate dehydratase MDE1 (MDE1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MDE1 ( NM_001181682.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012558.3 Saccharomyces cerevisiae S288C methylthioribulose 1-phosphate dehydratase MDE1 (MDE1), partial mRNA.
    Price & Availability↑
    CloneID OSi02723
    Clone ID Related Accession (Same CDS sequence) NM_001181682.3
    Accession Version NM_001181682.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product methylthioribulose 1-phosphate dehydratase MDE1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). On Jul 30, 2012 this sequence version replaced NM_001181682.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCTTCAC AAGATGTTCT GATACATTCA GATGATCCGT GCCATCCAGC AAATCTCATT 
    TGCACGCTAT GCAAGCAGTT TTTTCATAAT AATTGGTGCA CCGGAACTGG AGGTGGGATT 
    TCAATAAAAG ATCCTAACAC GAATTATTAT TATCTCGCCC CATCTGGGGT GCAGAAGGAG 
    AAGATGATAC CTGAGGATTT GTTTGTCATG GACGCTCAGA CATTAGAGTA TTTGCGCTCT 
    CCCAAGCTTT ACAAGCCAAG CGCATGTACT CCATTGTTCT TAGCGTGCTA CCAAAAGAAA 
    AATGCAGGTG CTATAATACA TACACACTCC CAAAATGCTG TGATATGCTC CTTGCTATTT 
    GGCGATGAGT TCAGGATTGC TAACATCGAA CAAATCAAAG CAATTCCAAG CGGAAAGGTG 
    GACCCTGTGA CCAAGAAACC CATGGCGCTA TCGTTTTTTG ATACGCTGAA GATTCCCATC 
    ATTGAAAACA TGGCGCATGA GGACGAGTTG ATCGATGACT TGCACAAGAC TTTTAAAGAT 
    TATCCAGACA CATGCGCCGT AATTGTGAGG AGACATGGCA TTTTTGTTTG GGGTCCGACA 
    ATCGATAAGG CCAAAATTTT CAACGAAGCC ATCGATTATT TGATGGAGCT GGCCATAAAG 
    ATGTACCAAA TGGGGATTCC TCCCGACTGT GGCATTGGCG AAGAGAAGAA GCATCTGAAG 
    ATGGCCAGTC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_012558.3
    CDS1..735
    Translation

    Target ORF information:

    RefSeq Version NM_001181682.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C methylthioribulose 1-phosphate dehydratase MDE1 (MDE1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181682.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCTTCAC AAGATGTTCT GATACATTCA GATGATCCGT GCCATCCAGC AAATCTCATT 
    TGCACGCTAT GCAAGCAGTT TTTTCATAAT AATTGGTGCA CCGGAACTGG AGGTGGGATT 
    TCAATAAAAG ATCCTAACAC GAATTATTAT TATCTCGCCC CATCTGGGGT GCAGAAGGAG 
    AAGATGATAC CTGAGGATTT GTTTGTCATG GACGCTCAGA CATTAGAGTA TTTGCGCTCT 
    CCCAAGCTTT ACAAGCCAAG CGCATGTACT CCATTGTTCT TAGCGTGCTA CCAAAAGAAA 
    AATGCAGGTG CTATAATACA TACACACTCC CAAAATGCTG TGATATGCTC CTTGCTATTT 
    GGCGATGAGT TCAGGATTGC TAACATCGAA CAAATCAAAG CAATTCCAAG CGGAAAGGTG 
    GACCCTGTGA CCAAGAAACC CATGGCGCTA TCGTTTTTTG ATACGCTGAA GATTCCCATC 
    ATTGAAAACA TGGCGCATGA GGACGAGTTG ATCGATGACT TGCACAAGAC TTTTAAAGAT 
    TATCCAGACA CATGCGCCGT AATTGTGAGG AGACATGGCA TTTTTGTTTG GGGTCCGACA 
    ATCGATAAGG CCAAAATTTT CAACGAAGCC ATCGATTATT TGATGGAGCT GGCCATAAAG 
    ATGTACCAAA TGGGGATTCC TCCCGACTGT GGCATTGGCG AAGAGAAGAA GCATCTGAAG 
    ATGGCCAGTC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
    The EMBO journal15(9)2031-49(1996 May)
    Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L


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