NIT2 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following NIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02589 NM_001181559.1
Latest version!
Saccharomyces cerevisiae S288C putative hydrolase (NIT2), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSi02589
    Clone ID Related Accession (Same CDS sequence) NM_001181559.1
    Accession Version NM_001181559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product putative hydrolase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTAGTA AATTAAAACG GGTTGCTGTC GCACAATTAT GTTCATCCGC TGACCTGACC 
    AAAAACTTAA AAGTGGTAAA AGAATTGATA TCTGAAGCCA TTCAAAAAAA GGCGGATGTC
    GTTTTTCTCC CTGAGGCAAG TGACTATCTT TCTCAAAACC CTCTTCATTC CAGGTATTTG
    GCACAGAAAA GCCCCAAATT TATTCGGCAA TTACAGTCAA GCATTACCGA CCTTGTCAGA
    GATAATTCAC GGAATATAGA TGTTTCCATT GGGGTACACT TGCCGCCCTC AGAACAAGAT
    TTACTTGAAG GCAATGATCG AGTCAGAAAC GTTCTATTAT ATATCGATCA TGAAGGGAAA
    ATATTGCAAG AATACCAAAA ACTGCATTTG TTTGACGTAG ACGTGCCAAA TGGACCGATA
    TTGAAAGAAT CAAAATCTGT CCAGCCTGGC AAAGCCATTC CTGACATTAT AGAGTCTCCA
    TTGGGAAAAT TGGGCAGTGC CATTTGTTAT GATATTCGCT TTCCCGAATT TTCTTTGAAA
    TTACGATCAA TGGGGGCTGA AATCTTATGT TTTCCCAGTG CGTTCACTAT TAAAACAGGG
    GAAGCACATT GGGAGTTACT AGGCAGAGCA AGAGCGGTTG ACACTCAATG CTATGTGCTC
    ATGCCAGGGC AGGTTGGAAT GCACGATCTC AGTGATCCAG AGTGGGAGAA ACAATCACAT
    ATGAGCGCTC TCGAAAAAAG CTCCAGGAGA GAATCTTGGG GACACTCAAT GGTCATTGAT
    CCTTGGGGTA AAATAATAGC GCATGCCGAT CCCTCTACTG TTGGACCACA ACTAATTCTC
    GCTGATTTGG ATCGTGAGCT TTTGCAAGAA ATAAGGAACA AAATGCCCTT ATGGAACCAG
    AGAAGAGACG ATTTATTTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_012409.1
    CDS1..924
    Translation

    Target ORF information:

    RefSeq Version NM_001181559.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C putative hydrolase (NIT2), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001181559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTAGTA AATTAAAACG GGTTGCTGTC GCACAATTAT GTTCATCCGC TGACCTGACC 
    AAAAACTTAA AAGTGGTAAA AGAATTGATA TCTGAAGCCA TTCAAAAAAA GGCGGATGTC
    GTTTTTCTCC CTGAGGCAAG TGACTATCTT TCTCAAAACC CTCTTCATTC CAGGTATTTG
    GCACAGAAAA GCCCCAAATT TATTCGGCAA TTACAGTCAA GCATTACCGA CCTTGTCAGA
    GATAATTCAC GGAATATAGA TGTTTCCATT GGGGTACACT TGCCGCCCTC AGAACAAGAT
    TTACTTGAAG GCAATGATCG AGTCAGAAAC GTTCTATTAT ATATCGATCA TGAAGGGAAA
    ATATTGCAAG AATACCAAAA ACTGCATTTG TTTGACGTAG ACGTGCCAAA TGGACCGATA
    TTGAAAGAAT CAAAATCTGT CCAGCCTGGC AAAGCCATTC CTGACATTAT AGAGTCTCCA
    TTGGGAAAAT TGGGCAGTGC CATTTGTTAT GATATTCGCT TTCCCGAATT TTCTTTGAAA
    TTACGATCAA TGGGGGCTGA AATCTTATGT TTTCCCAGTG CGTTCACTAT TAAAACAGGG
    GAAGCACATT GGGAGTTACT AGGCAGAGCA AGAGCGGTTG ACACTCAATG CTATGTGCTC
    ATGCCAGGGC AGGTTGGAAT GCACGATCTC AGTGATCCAG AGTGGGAGAA ACAATCACAT
    ATGAGCGCTC TCGAAAAAAG CTCCAGGAGA GAATCTTGGG GACACTCAAT GGTCATTGAT
    CCTTGGGGTA AAATAATAGC GCATGCCGAT CCCTCTACTG TTGGACCACA ACTAATTCTC
    GCTGATTTGG ATCGTGAGCT TTTGCAAGAA ATAAGGAACA AAATGCCCTT ATGGAACCAG
    AGAAGAGACG ATTTATTTCA TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
    The EMBO journal15(9)2031-49(1996 May)
    Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L