ZUO1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Ssz1 is catalytically inert and cannot adopt the closed conformation, but the substrate binding domain beta is completed by Zuo1.
Title: Structural insights into a unique Hsp70-Hsp40 interaction in the eukaryotic ribosome-associated complex.

This study presents new structural and cross-linking data allowing more precise positioning of Saccharomyces cerevisiae ribosome-associated J protein-Hsp70 chaperone Zuo1 near the 60S polypeptide-exit site and suggesting interactions of Zuo1 with the ribosomal protein eL31 and 25S rRNA helix 24.
Title: Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunits.

The yeast ribosome-associated complex is composed of HSP40 (Zuotin) and HSP70 (Ssz1). RAC stabilizes the 80S ribosome in nonrotated, classical conformation.
Title: Structural basis for interaction of a cotranslational chaperone with the eukaryotic ribosome.

Unfolding of Zuo1's C-terminal helical bundle domain results in ribosome dissociation followed by activation of Pdr1 via a direct interaction.
Title: Unfolding of the C-terminal domain of the J-protein Zuo1 releases autoinhibition and activates Pdr1-dependent transcription.

study presents structural analyses of the ribosome-associated complex (RAC) consisting (Hsp70) Ssz1 and the Hsp40 Zuo1; a unique alpha-helical domain in Zuo1 mediates ribosome interaction of RAC near the ribosomal proteins L22e and L31e and ribosomal RNA helix H59
Title: Structural characterization of a eukaryotic chaperone--the ribosome-associated complex.