RAD54 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD54
Entrez Gene ID	852713
Full Name	DNA-dependent ATPase RAD54
Synonyms	XRS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181028.1	NP_011352.1	DNA-dependent ATPase RAD54

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	rad54l	NP_957438.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008748	XP_557901.3
Arabidopsis thaliana (thale cress)	RAD54	NP_188552.3
Xenopus tropicalis (tropical clawed frog)	rad54l	NP_001001241.1
Pan troglodytes (chimpanzee)	RAD54L	XP_513146.3
Bos taurus (cattle)	RAD54L	NP_001124238.1
Mus musculus (house mouse)	Rad54l	NP_033041.3
Gallus gallus (chicken)	RAD54L	XP_422447.3
Caenorhabditis elegans (roundworm)	rad-54	NP_492438.1
Saccharomyces cerevisiae (baker's yeast)	RAD54	NP_011352.1
Kluyveromyces lactis	KLLA0A03069g	XP_451134.1
Eremothecium gossypii	AGOS_AEL297W	NP_984563.2
Drosophila melanogaster (fruit fly)	okr	NP_476661.1
Magnaporthe oryzae (rice blast fungus)	MGG_03549	XP_003716366.1
Neurospora crassa	NCU11255	XP_959470.1
Oryza sativa (rice)	Os02g0762800	NP_001048207.2
Homo sapiens (human)	RAD54L	NP_003570.2
Canis lupus familiaris (dog)	RAD54L	XP_532592.3
Rattus norvegicus (Norway rat)	Rad54l	NP_001128432.1
Schizosaccharomyces pombe (fission yeast)	rhp54	NP_594290.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ad51-mediated recombination occurring in the absence of Hed1 phosphorylation results in a significant increase in non-exchange chromosomes despite wild-type levels of crossovers, confirming previous results indicating a defect in crossover assurance. We propose that Rad51 function in meiosis is regulated in part by the coordinated phosphorylation of Rad54 and Hed1 by Mek1
Title: Mek1 Down Regulates Rad51 Activity during Yeast Meiosis by Phosphorylation of Hed1.

Rad54 acts as an hDNA pump that drives D loop formation while simultaneously removing Rad51 from hDNA, consolidating both ATP-dependent activities of Rad54 into a single mechanistic step.
Title: Rad54 functions as a heteroduplex DNA pump modulated by its DNA substrates and Rad51 during D loop formation.

SN specifically targets the RAD54 ATPase, but not DNA binding, through direct interaction with RAD54 and generation of reactive oxygen species.
Title: Analysis of the activities of RAD54, a SWI2/SNF2 protein, using a specific small-molecule inhibitor.

Our study identified a novel sequence motif critical for Rad54 function and showed that even perfect matches to the PIP box consensus may not necessarily identify PCNA interaction sites.
Title: A conserved sequence extending motif III of the motor domain in the Snf2-family DNA translocase Rad54 is critical for ATPase activity.

Rad51-Rad54 function together to promote intersister DNA strand exchange, whereas Dmc1-Tid1 tilt the bias toward interhomolog DNA strand exchange.
Title: Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination.

The following RAD54 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD54 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02088	NM_001181028.1 Latest version!	Saccharomyces cerevisiae S288C DNA-dependent ATPase RAD54 (RAD54), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD54 ( NM_001181028.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011352.1 Saccharomyces cerevisiae S288C DNA-dependent ATPase RAD54 (RAD54), partial mRNA.

Price & Availability↑

CloneID	OSi02088
Clone ID Related Accession (Same CDS sequence)	NM_001181028.1
Accession Version	NM_001181028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-dependent ATPase RAD54
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCAAGAC GCAGATTACC AGACAGACCA CCAAATGGAA TAGGAGCCGG TGAACGGCCG 
AGACTGGTAC CTAGGCCTAT TAACGTACAA GACTCGGTGA ACCGACTAAC GAAACCGTTC 
AGGGTCCCGT ACAAGAACAC GCACATCCCG CCCGCTGCTG GTAGAATCGC CACCGGGTCT 
GATAATATCG TAGGAGGAAG GAGCTTGAGG AAAAGATCAG CGACTGTATG TTATTCCGGC 
TTGGATATAA ATGCGGACGA AGCAGAGTAC AACAGCCAAG ACATAAGTTT TTCTCAGTTG 
ACTAAACGAC GGAAGGATGC TCTTAGTGCT CAAAGGTTGG CCAAGGATCC GACAAGACTG 
AGTCATATCC AGTACACTTT GAGAAGATCT TTCACTGTGC CAATCAAGGG ATATGTACAA 
AGACACAGTC TGCCATTAAC GTTAGGAATG AAAAAAAAAA TTACTCCAGA ACCTCGACCT 
CTACATGACC CTACAGACGA ATTTGCCATA GTGCTTTATG ACCCGTCCGT TGACGGTGAA 
ATGATTGTCC ATGACACATC TATGGATAAC AAGGAAGAAG AATCAAAGAA AATGATCAAA 
AGCACACAGG AAAAGGATAA TATTAACAAG GAAAAGAATA GCCAGGAAGA AAGACCCACA 
CAAAGAATAG GACGCCATCC AGCTTTGATG ACAAATGGTG TGAGAAACAA ACCGTTACGT 
GAGCTACTCG GTGATTCGGA AAACTCTGCG GAAAACAAGA AGAAATTTGC TAGTGTCCCT 
GTGGTTATTG ATCCAAAACT GGCCAAGATT TTAAGACCCC ATCAAGTCGA AGGGGTGAGA 
TTCTTATACC GTTGTGTCAC AGGGCTTGTC ATGAAAGATT ACTTAGAAGC TGAGGCGTTC 
AACACTTCAA GCGAAGATCC CTTAAAGAGC GACGAGAAGG CACTTACTGA ATCTCAAAAG 
ACTGAACAAA ACAATAGAGG GGCCTACGGC TGCATCATGG CCGATGAAAT GGGGCTGGGT 
AAGACATTGC AGTGTATAGC ACTTATGTGG ACATTATTAA GACAGGGCCC GCAGGGTAAA 
AGACTTATCG ACAAATGTAT CATTGTTTGT CCATCTTCTT TAGTCAACAA CTGGGCTAAT 
GAATTGATAA AATGGTTGGG TCCCAATACT CTAACTCCGT TAGCGGTTGA TGGGAAAAAA 
TCTTCAATGG GTGGTGGTAA TACAACAGTT TCGCAAGCTA TTCATGCCTG GGCTCAGGCA 
CAGGGAAGAA ATATTGTTAA GCCCGTTTTA ATCATATCGT ATGAAACATT ACGTCGAAAT 
GTTGATCAAT TGAAAAACTG CAATGTGGGT TTAATGCTGG CAGATGAAGG GCACCGTTTG 
AAAAACGGTG ACTCTTTAAC ATTCACAGCT TTGGATAGCA TCAGTTGTCC TAGAAGAGTT 
ATATTATCTG GTACACCCAT TCAAAACGAT CTTTCCGAAT ATTTTGCTCT ACTGAGTTTT 
TCGAATCCTG GCTTATTAGG TTCCAGAGCA GAATTTAGGA AAAATTTTGA GAATCCAATT 
TTGCGAGGGC GTGATGCTGA TGCTACTGAT AAAGAAATCA CAAAGGGCGA GGCGCAATTA 
CAGAAGCTAT CTACCATTGT CTCCAAATTT ATTATCCGTC GTACTAACGA TATTTTGGCC 
AAATATTTGC CTTGCAAGTA TGAGCACGTT ATTTTCGTAA ACTTGAAGCC GTTGCAGAAC 
GAGCTCTACA ATAAATTAAT TAAGTCAAGA GAAGTGAAAA AAGTAGTAAA AGGTGTTGGT 
GGGTCTCAGC CCCTGAGGGC TATTGGTATT TTAAAGAAGC TATGTAATCA CCCTAATCTC 
TTGAATTTTG AAGATGAATT TGACGATGAA GATGATTTGG AACTACCTGA CGATTATAAT 
ATGCCGGGTT CAAAAGCCAG GGACGTACAA ACAAAATATT CAGCTAAATT TTCCATTTTG 
GAAAGATTTC TCCATAAGAT AAAGACCGAA TCCGATGATA AGATTGTTTT GATCTCCAAC 
TATACCCAAA CCTTGGACCT CATTGAAAAA ATGTGTAGAT ATAAGCACTA CAGTGCTGTA 
CGGTTGGATG GTACAATGTC AATTAACAAA AGACAAAAAT TGGTGGATAG GTTCAACGAT 
CCTGAAGGCC AAGAGTTCAT CTTCCTCTTA AGTTCTAAAG CAGGTGGGTG TGGTATCAAT 
TTGATTGGTG CAAATCGATT AATTTTGATG GATCCGGATT GGAATCCTGC TGCTGATCAA 
CAAGCTTTGG CACGTGTTTG GAGAGACGGT CAAAAAAAGG ATTGTTTCAT TTACAGATTC 
ATATCGACTG GTACCATAGA GGAAAAAATC TTTCAAAGAC AATCTATGAA AATGAGTTTG 
AGTTCATGCG TGGTCGATGC GAAAGAAGAC GTTGAAAGGT TGTTTAGTTC TGACAATTTA 
AGACAGTTGT TTCAAAAAAA TGAGAATACG ATATGTGAAA CACACGAAAC ATACCATTGT 
AAACGTTGTA ATGCTCAGGG TAAACAATTA AAGAGAGCCC CTGCAATGTT ATATGGTGAT 
GCGACAACTT GGAATCATTT GAACCATGAC GCATTGGAAA AAACAAATGA TCATCTACTA 
AAAAACGAGC ATCATTACAA TGATATCAGT TTTGCATTTC AATATATTTC ACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011352.1
CDS	1..2697
Translation	MARRRLPDRPPNGIGAGERPRLVPRPINVQDSVNRLTKPFRVPYKNTHIPPAAGRIATGSDNIVGGRSLRKRSATVCYSGLDINADEAEYNSQDISFSQLTKRRKDALSAQRLAKDPTRLSHIQYTLRRSFTVPIKGYVQRHSLPLTLGMKKKITPEPRPLHDPTDEFAIVLYDPSVDGEMIVHDTSMDNKEEESKKMIKSTQEKDNINKEKNSQEERPTQRIGRHPALMTNGVRNKPLRELLGDSENSAENKKKFASVPVVIDPKLAKILRPHQVEGVRFLYRCVTGLVMKDYLEAEAFNTSSEDPLKSDEKALTESQKTEQNNRGAYGCIMADEMGLGKTLQCIALMWTLLRQGPQGKRLIDKCIIVCPSSLVNNWANELIKWLGPNTLTPLAVDGKKSSMGGGNTTVSQAIHAWAQAQGRNIVKPVLIISYETLRRNVDQLKNCNVGLMLADEGHRLKNGDSLTFTALDSISCPRRVILSGTPIQNDLSEYFALLSFSNPGLLGSRAEFRKNFENPILRGRDADATDKEITKGEAQLQKLSTIVSKFIIRRTNDILAKYLPCKYEHVIFVNLKPLQNELYNKLIKSREVKKVVKGVGGSQPLRAIGILKKLCNHPNLLNFEDEFDDEDDLELPDDYNMPGSKARDVQTKYSAKFSILERFLHKIKTESDDKIVLISNYTQTLDLIEKMCRYKHYSAVRLDGTMSINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGINLIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFISTGTIEEKIFQRQSMKMSLSSCVVDAKEDVERLFSSDNLRQLFQKNENTICETHETYHCKRCNAQGKQLKRAPAMLYGDATTWNHLNHDALEKTNDHLLKNEHHYNDISFAFQYISH

Target ORF information:

RefSeq Version	NM_001181028.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-dependent ATPase RAD54 (RAD54), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181028.1

ORF Insert Sequence:

ATGGCAAGAC GCAGATTACC AGACAGACCA CCAAATGGAA TAGGAGCCGG TGAACGGCCG 
AGACTGGTAC CTAGGCCTAT TAACGTACAA GACTCGGTGA ACCGACTAAC GAAACCGTTC 
AGGGTCCCGT ACAAGAACAC GCACATCCCG CCCGCTGCTG GTAGAATCGC CACCGGGTCT 
GATAATATCG TAGGAGGAAG GAGCTTGAGG AAAAGATCAG CGACTGTATG TTATTCCGGC 
TTGGATATAA ATGCGGACGA AGCAGAGTAC AACAGCCAAG ACATAAGTTT TTCTCAGTTG 
ACTAAACGAC GGAAGGATGC TCTTAGTGCT CAAAGGTTGG CCAAGGATCC GACAAGACTG 
AGTCATATCC AGTACACTTT GAGAAGATCT TTCACTGTGC CAATCAAGGG ATATGTACAA 
AGACACAGTC TGCCATTAAC GTTAGGAATG AAAAAAAAAA TTACTCCAGA ACCTCGACCT 
CTACATGACC CTACAGACGA ATTTGCCATA GTGCTTTATG ACCCGTCCGT TGACGGTGAA 
ATGATTGTCC ATGACACATC TATGGATAAC AAGGAAGAAG AATCAAAGAA AATGATCAAA 
AGCACACAGG AAAAGGATAA TATTAACAAG GAAAAGAATA GCCAGGAAGA AAGACCCACA 
CAAAGAATAG GACGCCATCC AGCTTTGATG ACAAATGGTG TGAGAAACAA ACCGTTACGT 
GAGCTACTCG GTGATTCGGA AAACTCTGCG GAAAACAAGA AGAAATTTGC TAGTGTCCCT 
GTGGTTATTG ATCCAAAACT GGCCAAGATT TTAAGACCCC ATCAAGTCGA AGGGGTGAGA 
TTCTTATACC GTTGTGTCAC AGGGCTTGTC ATGAAAGATT ACTTAGAAGC TGAGGCGTTC 
AACACTTCAA GCGAAGATCC CTTAAAGAGC GACGAGAAGG CACTTACTGA ATCTCAAAAG 
ACTGAACAAA ACAATAGAGG GGCCTACGGC TGCATCATGG CCGATGAAAT GGGGCTGGGT 
AAGACATTGC AGTGTATAGC ACTTATGTGG ACATTATTAA GACAGGGCCC GCAGGGTAAA 
AGACTTATCG ACAAATGTAT CATTGTTTGT CCATCTTCTT TAGTCAACAA CTGGGCTAAT 
GAATTGATAA AATGGTTGGG TCCCAATACT CTAACTCCGT TAGCGGTTGA TGGGAAAAAA 
TCTTCAATGG GTGGTGGTAA TACAACAGTT TCGCAAGCTA TTCATGCCTG GGCTCAGGCA 
CAGGGAAGAA ATATTGTTAA GCCCGTTTTA ATCATATCGT ATGAAACATT ACGTCGAAAT 
GTTGATCAAT TGAAAAACTG CAATGTGGGT TTAATGCTGG CAGATGAAGG GCACCGTTTG 
AAAAACGGTG ACTCTTTAAC ATTCACAGCT TTGGATAGCA TCAGTTGTCC TAGAAGAGTT 
ATATTATCTG GTACACCCAT TCAAAACGAT CTTTCCGAAT ATTTTGCTCT ACTGAGTTTT 
TCGAATCCTG GCTTATTAGG TTCCAGAGCA GAATTTAGGA AAAATTTTGA GAATCCAATT 
TTGCGAGGGC GTGATGCTGA TGCTACTGAT AAAGAAATCA CAAAGGGCGA GGCGCAATTA 
CAGAAGCTAT CTACCATTGT CTCCAAATTT ATTATCCGTC GTACTAACGA TATTTTGGCC 
AAATATTTGC CTTGCAAGTA TGAGCACGTT ATTTTCGTAA ACTTGAAGCC GTTGCAGAAC 
GAGCTCTACA ATAAATTAAT TAAGTCAAGA GAAGTGAAAA AAGTAGTAAA AGGTGTTGGT 
GGGTCTCAGC CCCTGAGGGC TATTGGTATT TTAAAGAAGC TATGTAATCA CCCTAATCTC 
TTGAATTTTG AAGATGAATT TGACGATGAA GATGATTTGG AACTACCTGA CGATTATAAT 
ATGCCGGGTT CAAAAGCCAG GGACGTACAA ACAAAATATT CAGCTAAATT TTCCATTTTG 
GAAAGATTTC TCCATAAGAT AAAGACCGAA TCCGATGATA AGATTGTTTT GATCTCCAAC 
TATACCCAAA CCTTGGACCT CATTGAAAAA ATGTGTAGAT ATAAGCACTA CAGTGCTGTA 
CGGTTGGATG GTACAATGTC AATTAACAAA AGACAAAAAT TGGTGGATAG GTTCAACGAT 
CCTGAAGGCC AAGAGTTCAT CTTCCTCTTA AGTTCTAAAG CAGGTGGGTG TGGTATCAAT 
TTGATTGGTG CAAATCGATT AATTTTGATG GATCCGGATT GGAATCCTGC TGCTGATCAA 
CAAGCTTTGG CACGTGTTTG GAGAGACGGT CAAAAAAAGG ATTGTTTCAT TTACAGATTC 
ATATCGACTG GTACCATAGA GGAAAAAATC TTTCAAAGAC AATCTATGAA AATGAGTTTG 
AGTTCATGCG TGGTCGATGC GAAAGAAGAC GTTGAAAGGT TGTTTAGTTC TGACAATTTA 
AGACAGTTGT TTCAAAAAAA TGAGAATACG ATATGTGAAA CACACGAAAC ATACCATTGT 
AAACGTTGTA ATGCTCAGGG TAAACAATTA AAGAGAGCCC CTGCAATGTT ATATGGTGAT 
GCGACAACTT GGAATCATTT GAACCATGAC GCATTGGAAA AAACAAATGA TCATCTACTA 
AAAAACGAGC ATCATTACAA TGATATCAGT TTTGCATTTC AATATATTTC ACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RAD54 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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