PMR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PMR1
Entrez Gene ID	852709
Full Name	Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1
Synonyms	BSD1,LDB1,SSC1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181032.1	NP_011348.1	Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ATP2C1	XP_001146246.2
Canis lupus familiaris (dog)	ATP2C1	XP_005634472.1
Bos taurus (cattle)	ATP2C1	NP_786979.1
Gallus gallus (chicken)	ATP2C1	XP_426010.4
Kluyveromyces lactis	KLLA0A03157g	XP_451138.1
	ATP2C1	XP_002803047.1
Caenorhabditis elegans (roundworm)	pmr-1	NP_001021862.1
Eremothecium gossypii	AGOS_AEL301W	NP_984559.2
Schizosaccharomyces pombe (fission yeast)	pmr1	NP_595098.1
Mus musculus (house mouse)	Atp2c1	NP_001240760.1
Drosophila melanogaster (fruit fly)	SPoCk	NP_730742.1
Magnaporthe oryzae (rice blast fungus)	MGG_11727	XP_003709985.1
Neurospora crassa	NCU03292	XP_964218.1
Rattus norvegicus (Norway rat)	Atp2c1	NP_571982.2
Danio rerio (zebrafish)	atp2c1	XP_003200287.2
Homo sapiens (human)	ATP2C1	NP_001186110.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012115	XP_320413.3
Saccharomyces cerevisiae (baker's yeast)	PMR1	NP_011348.1
Xenopus tropicalis (tropical clawed frog)	atp2c1	XP_002937789.2

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Tettelin H, Agostoni Carbone ML, Albermann K, Albers M, Arroyo J, Backes U, Barreiros T, Bertani I, Bjourson AJ, Br?ckner M, Bruschi CV, Carignani G, Castagnoli L, Cerdan E, Clemente ML, Coblenz A, Coglievina M, Coissac E, Defoor E, Del Bino S, Delius H, Delneri D, de Wergifosse P, Dujon B, Kleine K
Nature387(6632 Suppl)81-4(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that calcium stimulates endoplasmic reticulum and late endosome/trans- to cis-Golgi manganese delivery and bypasses the need for Ca(2+)/Mn(2+)-transporting P-type ATPase Pmr1.
Title: Manganese redistribution by calcium-stimulated vesicle trafficking bypasses the need for P-type ATPase function.

Pmr1p has a central role in cell survival in a Cd2+-rich environment.
Title: The Pmr1 protein, the major yeast Ca2+-ATPase in the Golgi, regulates intracellular levels of the cadmium ion.

PMR1 functions involved drug resistance and Golgi apparatus
Title: A phenomics approach in yeast links proton and calcium pump function in the Golgi.

Results establish that Pmr1-dependent Ca2+ and/or Mn2+ ion homeostasis is necessary for TOR signaling.
Title: Pmr1, a Golgi Ca2+/Mn2+-ATPase, is a regulator of the target of rapamycin (TOR) signaling pathway in yeast.

The following PMR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PMR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02085	NM_001181032.1 Latest version!	Saccharomyces cerevisiae S288C Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1 (PMR1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PMR1 ( NM_001181032.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011348.1 Saccharomyces cerevisiae S288C Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1 (PMR1), partial mRNA.

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CloneID	OSi02085
Clone ID Related Accession (Same CDS sequence)	NM_001181032.1
Accession Version	NM_001181032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTGACA ATCCATTTAA TGCGAGTCTT CTTGACGAGG ACTCAAACCG TGAGAGAGAA 
ATACTAGATG CCACAGCAGA GGCCCTTTCG AAACCAAGCC CTTCTTTAGA GTATTGTACT 
TTATCCGTGG ACGAAGCTCT AGAAAAACTG GACACTGACA AAAACGGTGG TTTACGATCA 
TCTAACGAGG CCAACAATAG GAGATCACTT TATGGCCCCA ATGAAATAAC CGTAGAAGAT 
GATGAAAGTC TTTTCAAGAA GTTCTTGTCA AATTTCATTG AGGATCGAAT GATTCTACTT 
TTAATAGGAT CCGCAGTGGT CTCTCTTTTT ATGGGTAACA TTGATGATGC TGTTAGTATC 
ACACTGGCCA TTTTCATAGT TGTCACTGTC GGTTTTGTCC AAGAATATAG GTCTGAAAAA 
TCTCTAGAAG CGTTGAATAA ATTGGTTCCT GCTGAATGTC ACTTAATGAG ATGTGGTCAA 
GAGAGTCATG TACTGGCTTC CACCTTGGTT CCTGGTGATT TAGTGCACTT CAGAATAGGT 
GACAGAATCC CCGCAGACAT TAGAATTATT GAAGCAATCG ATTTATCCAT CGATGAAAGT 
AATTTAACTG GTGAAAATGA ACCGGTACAT AAAACCTCAC AAACGATCGA AAAATCTTCC 
TTTAACGATC AGCCTAATTC AATTGTACCG ATTTCTGAGA GATCTTGTAT AGCTTATATG 
GGTACATTAG TCAAGGAAGG TCATGGTAAG GGTATCGTCG TAGGAACAGG TACAAACACA 
TCCTTTGGTG CCGTTTTTGA AATGATGAAT AATATTGAAA AACCGAAGAC TCCATTGCAG 
TTAACAATGG ACAAATTGGG AAAGGACTTG TCACTGGTTA GCTTCATAGT TATTGGTATG 
ATTTGTTTAG TTGGTATCAT ACAAGGTAGA TCTTGGTTAG AAATGTTCCA AATATCGGTA 
TCCTTAGCGG TTGCTGCTAT TCCAGAAGGG TTACCAATTA TTGTCACTGT TACTTTGGCA 
TTGGGTGTTC TGAGAATGGC CAAGCGTAAA GCCATCGTGA GAAGGTTACC AAGTGTCGAA 
ACTTTAGGCT CTGTCAACGT TATCTGCTCC GACAAAACAG GTACACTAAC CTCAAACCAC 
ATGACCGTAT CTAAACTTTG GTGCTTGGAC AGTATGTCCA ATAAGCTAAA CGTCCTCTCA 
TTAGACAAAA ATAAGAAGAC TAAAAATTCT AATGGAAATT TGAAAAACTA TTTGACTGAA 
GACGTTAGGG AAACTCTAAC TATCGGTAAT CTCTGTAATA ATGCATCTTT CTCTCAAGAA 
CATGCCATAT TTCTGGGAAA TCCTACTGAT GTAGCTCTTT TAGAGCAATT GGCAAACTTT 
GAAATGCCTG ATATCAGAAA CACCGTTCAA AAAGTTCAGG AACTTCCATT TAACTCGAAA 
AGAAAATTAA TGGCAACCAA GATTCTCAAC CCTGTCGACA ATAAGTGTAC AGTTTATGTT 
AAAGGTGCAT TTGAAAGAAT TCTTGAGTAC TCCACAAGTT ATTTGAAATC AAAGGGTAAA 
AAAACTGAAA AGTTGACTGA AGCCCAAAAA GCTACGATAA ATGAGTGCGC AAATTCTATG 
GCATCTGAAG GTTTGCGTGT CTTTGGATTT GCTAAACTAA CTTTGTCTGA TTCATCAACT 
CCTCTAACCG AAGACCTAAT CAAAGATTTA ACCTTTACTG GTTTAATCGG TATGAATGAC 
CCACCAAGAC CGAACGTTAA ATTTGCCATC GAACAATTAC TACAAGGTGG TGTCCATATT 
ATTATGATCA CTGGTGATTC TGAGAATACC GCAGTAAACA TTGCAAAACA AATTGGTATT 
CCAGTTATTG ATCCAAAGCT TTCCGTTTTA TCCGGTGATA AATTAGATGA AATGTCAGAT 
GATCAACTGG CCAATGTCAT CGACCACGTT AATATTTTTG CTCGTGCTAC GCCTGAGCAT 
AAGTTAAACA TTGTTCGTGC ATTAAGAAAG AGGGGTGATG TGGTAGCAAT GACTGGTGAT 
GGTGTTAACG ACGCTCCTGC GTTGAAACTT TCAGATATTG GTGTTTCTAT GGGTAGAATT 
GGTACAGATG TAGCCAAAGA AGCCTCAGAT ATGGTCTTAA CTGATGATGA CTTCAGTACT 
ATTTTAACTG CCATTGAAGA GGGTAAAGGT ATCTTTAATA ATATTCAGAA TTTCCTGACT 
TTTCAATTGT CTACTTCTGT TGCCGCACTA TCATTAGTTG CACTATCTAC AGCGTTTAAA 
CTACCCAATC CACTGAACGC AATGCAAATT CTTTGGATAA ATATTTTAAT GGATGGGCCA 
CCAGCTCAAT CCTTAGGTGT GGAACCTGTT GATCATGAAG TTATGAAAAA ACCTCCAAGA 
AAACGTACCG ATAAAATTTT GACCCATGAT GTAATGAAAC GTTTACTAAC CACCGCGGCC 
TGTATCATCG TTGGGACAGT TTACATTTTT GTTAAAGAGA TGGCCGAAGA TGGTAAAGTA 
ACTGCTAGAG ATACTACTAT GACATTTACT TGTTTTGTTT TTTTTGATAT GTTTAATGCT 
TTGGCCTGCA GACATAACAC AAAGTCAATC TTCGAAATCG GCTTTTTCAC GAACAAAATG 
TTCAACTACG CCGTTGGACT GTCTCTGTTA GGTCAAATGT GCGCTATATA TATACCATTT 
TTCCAAAGTA TCTTTAAAAC TGAGAAACTT GGTATCTCTG ATATACTATT GTTATTGCTC 
ATCAGCAGTA GCGTTTTCAT CGTTGATGAA TTGAGAAAAT TGTGGACGAG GAAAAAGAAT 
GAAGAAGACT CAACGTATTT CTCAAATGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011348.1
CDS	1..2853
Translation	MSDNPFNASLLDEDSNREREILDATAEALSKPSPSLEYCTLSVDEALEKLDTDKNGGLRSSNEANNRRSLYGPNEITVEDDESLFKKFLSNFIEDRMILLLIGSAVVSLFMGNIDDAVSITLAIFIVVTVGFVQEYRSEKSLEALNKLVPAECHLMRCGQESHVLASTLVPGDLVHFRIGDRIPADIRIIEAIDLSIDESNLTGENEPVHKTSQTIEKSSFNDQPNSIVPISERSCIAYMGTLVKEGHGKGIVVGTGTNTSFGAVFEMMNNIEKPKTPLQLTMDKLGKDLSLVSFIVIGMICLVGIIQGRSWLEMFQISVSLAVAAIPEGLPIIVTVTLALGVLRMAKRKAIVRRLPSVETLGSVNVICSDKTGTLTSNHMTVSKLWCLDSMSNKLNVLSLDKNKKTKNSNGNLKNYLTEDVRETLTIGNLCNNASFSQEHAIFLGNPTDVALLEQLANFEMPDIRNTVQKVQELPFNSKRKLMATKILNPVDNKCTVYVKGAFERILEYSTSYLKSKGKKTEKLTEAQKATINECANSMASEGLRVFGFAKLTLSDSSTPLTEDLIKDLTFTGLIGMNDPPRPNVKFAIEQLLQGGVHIIMITGDSENTAVNIAKQIGIPVIDPKLSVLSGDKLDEMSDDQLANVIDHVNIFARATPEHKLNIVRALRKRGDVVAMTGDGVNDAPALKLSDIGVSMGRIGTDVAKEASDMVLTDDDFSTILTAIEEGKGIFNNIQNFLTFQLSTSVAALSLVALSTAFKLPNPLNAMQILWINILMDGPPAQSLGVEPVDHEVMKKPPRKRTDKILTHDVMKRLLTTAACIIVGTVYIFVKEMAEDGKVTARDTTMTFTCFVFFDMFNALACRHNTKSIFEIGFFTNKMFNYAVGLSLLGQMCAIYIPFFQSIFKTEKLGISDILLLLLISSSVFIVDELRKLWTRKKNEEDSTYFSNV

Target ORF information:

RefSeq Version	NM_001181032.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ca(2+)/Mn(2+)-transporting P-type ATPase PMR1 (PMR1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181032.1

ORF Insert Sequence:

ATGAGTGACA ATCCATTTAA TGCGAGTCTT CTTGACGAGG ACTCAAACCG TGAGAGAGAA 
ATACTAGATG CCACAGCAGA GGCCCTTTCG AAACCAAGCC CTTCTTTAGA GTATTGTACT 
TTATCCGTGG ACGAAGCTCT AGAAAAACTG GACACTGACA AAAACGGTGG TTTACGATCA 
TCTAACGAGG CCAACAATAG GAGATCACTT TATGGCCCCA ATGAAATAAC CGTAGAAGAT 
GATGAAAGTC TTTTCAAGAA GTTCTTGTCA AATTTCATTG AGGATCGAAT GATTCTACTT 
TTAATAGGAT CCGCAGTGGT CTCTCTTTTT ATGGGTAACA TTGATGATGC TGTTAGTATC 
ACACTGGCCA TTTTCATAGT TGTCACTGTC GGTTTTGTCC AAGAATATAG GTCTGAAAAA 
TCTCTAGAAG CGTTGAATAA ATTGGTTCCT GCTGAATGTC ACTTAATGAG ATGTGGTCAA 
GAGAGTCATG TACTGGCTTC CACCTTGGTT CCTGGTGATT TAGTGCACTT CAGAATAGGT 
GACAGAATCC CCGCAGACAT TAGAATTATT GAAGCAATCG ATTTATCCAT CGATGAAAGT 
AATTTAACTG GTGAAAATGA ACCGGTACAT AAAACCTCAC AAACGATCGA AAAATCTTCC 
TTTAACGATC AGCCTAATTC AATTGTACCG ATTTCTGAGA GATCTTGTAT AGCTTATATG 
GGTACATTAG TCAAGGAAGG TCATGGTAAG GGTATCGTCG TAGGAACAGG TACAAACACA 
TCCTTTGGTG CCGTTTTTGA AATGATGAAT AATATTGAAA AACCGAAGAC TCCATTGCAG 
TTAACAATGG ACAAATTGGG AAAGGACTTG TCACTGGTTA GCTTCATAGT TATTGGTATG 
ATTTGTTTAG TTGGTATCAT ACAAGGTAGA TCTTGGTTAG AAATGTTCCA AATATCGGTA 
TCCTTAGCGG TTGCTGCTAT TCCAGAAGGG TTACCAATTA TTGTCACTGT TACTTTGGCA 
TTGGGTGTTC TGAGAATGGC CAAGCGTAAA GCCATCGTGA GAAGGTTACC AAGTGTCGAA 
ACTTTAGGCT CTGTCAACGT TATCTGCTCC GACAAAACAG GTACACTAAC CTCAAACCAC 
ATGACCGTAT CTAAACTTTG GTGCTTGGAC AGTATGTCCA ATAAGCTAAA CGTCCTCTCA 
TTAGACAAAA ATAAGAAGAC TAAAAATTCT AATGGAAATT TGAAAAACTA TTTGACTGAA 
GACGTTAGGG AAACTCTAAC TATCGGTAAT CTCTGTAATA ATGCATCTTT CTCTCAAGAA 
CATGCCATAT TTCTGGGAAA TCCTACTGAT GTAGCTCTTT TAGAGCAATT GGCAAACTTT 
GAAATGCCTG ATATCAGAAA CACCGTTCAA AAAGTTCAGG AACTTCCATT TAACTCGAAA 
AGAAAATTAA TGGCAACCAA GATTCTCAAC CCTGTCGACA ATAAGTGTAC AGTTTATGTT 
AAAGGTGCAT TTGAAAGAAT TCTTGAGTAC TCCACAAGTT ATTTGAAATC AAAGGGTAAA 
AAAACTGAAA AGTTGACTGA AGCCCAAAAA GCTACGATAA ATGAGTGCGC AAATTCTATG 
GCATCTGAAG GTTTGCGTGT CTTTGGATTT GCTAAACTAA CTTTGTCTGA TTCATCAACT 
CCTCTAACCG AAGACCTAAT CAAAGATTTA ACCTTTACTG GTTTAATCGG TATGAATGAC 
CCACCAAGAC CGAACGTTAA ATTTGCCATC GAACAATTAC TACAAGGTGG TGTCCATATT 
ATTATGATCA CTGGTGATTC TGAGAATACC GCAGTAAACA TTGCAAAACA AATTGGTATT 
CCAGTTATTG ATCCAAAGCT TTCCGTTTTA TCCGGTGATA AATTAGATGA AATGTCAGAT 
GATCAACTGG CCAATGTCAT CGACCACGTT AATATTTTTG CTCGTGCTAC GCCTGAGCAT 
AAGTTAAACA TTGTTCGTGC ATTAAGAAAG AGGGGTGATG TGGTAGCAAT GACTGGTGAT 
GGTGTTAACG ACGCTCCTGC GTTGAAACTT TCAGATATTG GTGTTTCTAT GGGTAGAATT 
GGTACAGATG TAGCCAAAGA AGCCTCAGAT ATGGTCTTAA CTGATGATGA CTTCAGTACT 
ATTTTAACTG CCATTGAAGA GGGTAAAGGT ATCTTTAATA ATATTCAGAA TTTCCTGACT 
TTTCAATTGT CTACTTCTGT TGCCGCACTA TCATTAGTTG CACTATCTAC AGCGTTTAAA 
CTACCCAATC CACTGAACGC AATGCAAATT CTTTGGATAA ATATTTTAAT GGATGGGCCA 
CCAGCTCAAT CCTTAGGTGT GGAACCTGTT GATCATGAAG TTATGAAAAA ACCTCCAAGA 
AAACGTACCG ATAAAATTTT GACCCATGAT GTAATGAAAC GTTTACTAAC CACCGCGGCC 
TGTATCATCG TTGGGACAGT TTACATTTTT GTTAAAGAGA TGGCCGAAGA TGGTAAAGTA 
ACTGCTAGAG ATACTACTAT GACATTTACT TGTTTTGTTT TTTTTGATAT GTTTAATGCT 
TTGGCCTGCA GACATAACAC AAAGTCAATC TTCGAAATCG GCTTTTTCAC GAACAAAATG 
TTCAACTACG CCGTTGGACT GTCTCTGTTA GGTCAAATGT GCGCTATATA TATACCATTT 
TTCCAAAGTA TCTTTAAAAC TGAGAAACTT GGTATCTCTG ATATACTATT GTTATTGCTC 
ATCAGCAGTA GCGTTTTCAT CGTTGATGAA TTGAGAAAAT TGTGGACGAG GAAAAAGAAT 
GAAGAAGACT CAACGTATTT CTCAAATGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
Nature387(6632 Suppl)81-4(1997 May)
Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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PMR1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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