VHC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VHC1
Entrez Gene ID	852537
Full Name	Vhc1p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178583.3	NP_009794.3	Vhc1p

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC12A9	NP_064631.2
	SLC12A9	XP_001105778.2
Caenorhabditis elegans (roundworm)	T04B8.5	NP_001022305.1
Saccharomyces cerevisiae (baker's yeast)	VHC1	NP_009794.3
Kluyveromyces lactis	KLLA0F10549g	XP_455560.1
Eremothecium gossypii	AGOS_AFL025C	NP_985523.1
Mus musculus (house mouse)	Slc12a9	NP_113583.2
Gallus gallus (chicken)	SLC12A9	XP_003641839.2
Danio rerio (zebrafish)	slc12a9	NP_001122020.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010249	XP_319440.3
Xenopus tropicalis (tropical clawed frog)	LOC100498108	XP_002937395.2
Bos taurus (cattle)	SLC12A9	NP_001193215.1
Drosophila melanogaster (fruit fly)	CG10413	NP_609887.1
Schizosaccharomyces pombe (fission yeast)	can1	NP_595740.1
Neurospora crassa	NCU03784	XP_961130.1
Pan troglodytes (chimpanzee)	SLC12A9	XP_003318707.1
Canis lupus familiaris (dog)	SLC12A9	XP_005621083.1
Rattus norvegicus (Norway rat)	Slc12a9	NP_599232.1
Magnaporthe oryzae (rice blast fungus)	MGG_16806	XP_003712279.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of mammalian plasma-membrane Na+-K+-2Cl- co-transporter NKCC2 in yeast cells complements the phenotypes resulting from the deletion of the VHC1 gene.
Title: Human NKCC2 cation?Cl? co-transporter complements lack of Vhc1 transporter in yeast vacuolar membranes.

Vhc1 is a novel transporter belonging to the family of electroneutral cation-Cl(-) cotransporters which participates in the regulation of cation content and morphology of Saccharomyces cerevisiae vacuoles
Title: Vhc1, a novel transporter belonging to the family of electroneutral cation-Cl(-) cotransporters, participates in the regulation of cation content and morphology of Saccharomyces cerevisiae vacuoles.

The following VHC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VHC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01931	NM_001178583.3 Latest version!	Saccharomyces cerevisiae S288C Vhc1p (VHC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VHC1 ( NM_001178583.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009794.3 Saccharomyces cerevisiae S288C Vhc1p (VHC1), partial mRNA.

Price & Availability↑

CloneID	OSi01931
Clone ID Related Accession (Same CDS sequence)	NM_001178583.3
Accession Version	NM_001178583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Vhc1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178583.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTTAGTA GGTTTTATCA GATTCCAGGT ACGCATCGGC CATCGTCGGC AATATCTTCT 
TCAAATGAAT CTTCTTCACT GTTGTCTGCA AGGAGAATAA GTCAAACATA TTTCAATTAT 
CAGGCGACCC CTGAATGCCA AAAAGTTTCC TCTAAGTATG ATCCTGATAA CCCAAACAAA 
GATAAGTTGG GAACATACGA TGGGGTATTT GTGCCTACTG CTTTAAACGT ATTGTCTATC 
CTTATGTTTC TTCGTTTTGG CTTCATTTTG GGTCAGTTAG GTATTATATG CACCATCGGT 
CTTCTGCTAT TGAGTTACAC TATTAATCTT CTCACCACGT TAAGTATATC TGCTATATCT 
ACAAACGGGA CTGTTAGGGG CGGGGGTGCT TATTATATGA TTTCAAGAAG TTTAGGACCT 
GAATTTGGTG GATCCATTGG GCTTGTCTTT TTCTTAGGGC AGGTGTTCAA TGCAGGTATG 
AACGCAGTGG GTATTATCGA ACCTTTACTC TATAACTTGG GTTATTCTGC TCAAGGCGAG 
CCACCTGCAG CTTTGGGAGA GCTACTACCA AGAGGGCATT GGCACGAATT TACATATGCC 
ACGGTCATTC TTTTCTTATG TTTTTCTGTG GCTTTTGTTG GCTCGCAAAC AGTGTCAAGA 
GCAGGGAATA TTCTTTTTTT GGTACTGGCA GCTTCTATAT TTTCCATTCC ACTTTCTGCA 
TTGATAAGAT CGCCATTCAC TGAAGGCGGT ATCAGTTATA CAGGTCCTTC ATGGCAAACT 
TTTCATGATA ACCTGCTCCC TCACTTAACA AAAGGTGCAG CAGGTTCGTT ACTCAAAGGT 
AAGGAAACAT TCAATGATTT ATTTGGCGTT TTTTTTCCTG CTACAGCTGG TATATTCGCG 
GGCGCAGGAA TGTCAAGTGA ATTGAGAAAG CCCTCTAAAT CAATTCCTAA GGGTACCTTA 
TGGGGTTTAC TTTTCACTTT TATCTGTTAT GCCGTTGTTG TCTTCTCCAT GGGTTGTTCC 
ATTCCCAGAA GATCGTTATA TGATGAAGTA CAAATTATAC AGACGATCAG TTCCGTTCAG 
TGGGTTATAT TCATGGGTGA AATGGCTACT TCTCTTTTCT CCATCATAGT GGGGATGCTC 
GGTGCTGCTT ATGTATTAGA AGCAATTGCA AAGGATAACA TTATTCCTGG TTTGGAGATT 
TTTGCTCATT CACCGTTATA TTCATTGATT TTTACTTGGA TTCTAACACA GCTATGTTTG 
TTTTCAGACG TCAATAAAAT CGCCACATTT ATCACAATGA CATTCTTAAT GACGTTTGTG 
GTCATGAATT TGGCGTGCTT CCTATTAGGT ATTTCCTCTG CTCCCAATTT CAGGCCCTCT 
TTCAAATACT TTAACAGGTA TACTACAGCA ATTGGCGCAT TACTTTCTGT TGTTGCGATG 
TTAATAGTTG ATGGCATCTC AGCGTCTGTC TTGTTTTTGG CTATGATTTT GCTATTTTTA 
TTCATTCATT ATTTTTCACC ACCGAAGTCT TGGGGTGATG TGTCTCAAAG TTTAATATAT 
CATCAAGTGA GAAAGTATTT GCTTCGCCTA CGTCAAGACA ATATTAAATA CTGGAGGCCT 
CAAATATTGC TGTTTGTAGA TAATCCAAGG ACGAGCTGGA ACTTAATAAG GTTTTGCAAT 
CATTTGAAAA AAGGTGGTTT GTATATTTTA GGCCATGTTG CAGTAACTGC GGATTTTCCG 
AAGCAACTAA ATGAATTAAA AACTCAGCAG AAAGCTTGGA TGAAGATCAG AGACATGGCA 
GCAATAAAGG CTTTTGTCCA AGTTGGGACT GGTCCCTCCC TTATATGGGG AATTAGGAAT 
GTTTTTATTG GTTCCGGGCT TGGAGGTATG AAACCAAACA TTACTGTTGT CGGTTTTTTT 
GACCTCGAAA GTTATAGAAA GCACATCCCA CAAAGTCGCA GCCAAAATAA CCTTCAGAAA 
CAGGTGGAAA TAAAAGCTAC AGTGCCACGT AGTACATGCT CTGATGTCAA AATCAATGTT 
CCATTGCCTA CGGATGAGTG TAAAAATGAA ACCAAAGTTA ATGTACAACA ATGGGTTCAA 
ATTGTAGAAG ATCTATCACT GATGCAATCC AATATTGCCA TAGCGCATGG ATTCAAAAAT 
TTAGAGATAC CGAACAAAAG GGACAGCTGC TTTCCAAAAA AAACTATAGA TCTTTATCCC 
ATCCAAATGT GCGGAAAAGT TGAGGCAAAG GGTGACCAGC CTGCGGCTAT CACTACTAAC 
TTTGATACGT ATACACTGAT TCTACAATTA GCTGCCATTT TGGTTACTGT TCCTGAATGG 
AAGCGCACAC ATTCGCTTCG AGTTATTTTA TTTGTGGAAC AAGAATATCA TAGAACGAAC 
GAAACTCAAC GAATGAAAAA GCTATTACAG GTTCTAAGAA TTGACGCAGA GGTTTTGGTA 
GTATCTTTAG ATCAATTCAG AGTATACAAC ACAATTGTCA AGGGAGACCC AATCGTGTTT 
GATTATGTCA ACTCGAAATT GGCCGATAAT GAATGGTGGA AAGATTTGGT TGAAGCTCGT 
GATACATTAA AACCGAAGCG GAGGTTTTCC ACTATTGAAC CTCAAACGAT AGCAAAGCAA 
TTTACACAAT CAAGAAAATA TACGTCTGGG GTTCAGAAAT TGGGTGTCTC ATTTACAATG 
AATACGAGAA TGCCAACTAA CCGCATTGAT ACCCCATGCG AGAGTGAGGA CTCCGATTTG 
GATACAGATC TTACGTCAAT TCGTGATGCC TTCTCGGCAT CGACTAATAT TTCAGTAGGT 
AAGGACTTAA CGACCAAATC GAAGACCGGT TCCGACAGAA CAAATTTACT CGTAAAGAAT 
TTACAAAGCG ATGTGTCAAC ACAGTCGTTA AGACCCGTTT TCTCGAGTAA TACTTTGCCG 
AGGACGAGAG TCGTGGAAGA TGGCACGGGT GAACAGCCGA CACTGATCCC AATTGCCGAA 
CCTGACCTTT CGAATGGGAA CGGTACTGGG AGCGGTATTG GCAACGGCAA CAAATTAAAG 
AAACCGGTTC TTCCCGAATT ATCACCTTGT TGTTCAAAAG ATAGTTTAGT CACAGCGATG 
CAAAATTTAG GTTTCAATGA TCTTCCCAGC ACTGCTCAAC ATTTGGTTCT GAATGATGTG 
ATGACACAAA TGTCAAAGAG TTCGGATTTA ATTTTTTCAA CCTTGCCAGT TCCAGCTCTT 
GGAACACATG AAGATCACGA TGCAAGTTTA CAATATGTCG AGGACTTGGA TATTTGGTTG 
GAGGGCTTAC CTCCATGCAT GTTAATCAAT TCGCAAACCA TGACAGTAAC TACTGCATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009794.3
CDS	1..3363
Translation	MVSRFYQIPGTHRPSSAISSSNESSSLLSARRISQTYFNYQATPECQKVSSKYDPDNPNKDKLGTYDGVFVPTALNVLSILMFLRFGFILGQLGIICTIGLLLLSYTINLLTTLSISAISTNGTVRGGGAYYMISRSLGPEFGGSIGLVFFLGQVFNAGMNAVGIIEPLLYNLGYSAQGEPPAALGELLPRGHWHEFTYATVILFLCFSVAFVGSQTVSRAGNILFLVLAASIFSIPLSALIRSPFTEGGISYTGPSWQTFHDNLLPHLTKGAAGSLLKGKETFNDLFGVFFPATAGIFAGAGMSSELRKPSKSIPKGTLWGLLFTFICYAVVVFSMGCSIPRRSLYDEVQIIQTISSVQWVIFMGEMATSLFSIIVGMLGAAYVLEAIAKDNIIPGLEIFAHSPLYSLIFTWILTQLCLFSDVNKIATFITMTFLMTFVVMNLACFLLGISSAPNFRPSFKYFNRYTTAIGALLSVVAMLIVDGISASVLFLAMILLFLFIHYFSPPKSWGDVSQSLIYHQVRKYLLRLRQDNIKYWRPQILLFVDNPRTSWNLIRFCNHLKKGGLYILGHVAVTADFPKQLNELKTQQKAWMKIRDMAAIKAFVQVGTGPSLIWGIRNVFIGSGLGGMKPNITVVGFFDLESYRKHIPQSRSQNNLQKQVEIKATVPRSTCSDVKINVPLPTDECKNETKVNVQQWVQIVEDLSLMQSNIAIAHGFKNLEIPNKRDSCFPKKTIDLYPIQMCGKVEAKGDQPAAITTNFDTYTLILQLAAILVTVPEWKRTHSLRVILFVEQEYHRTNETQRMKKLLQVLRIDAEVLVVSLDQFRVYNTIVKGDPIVFDYVNSKLADNEWWKDLVEARDTLKPKRRFSTIEPQTIAKQFTQSRKYTSGVQKLGVSFTMNTRMPTNRIDTPCESEDSDLDTDLTSIRDAFSASTNISVGKDLTTKSKTGSDRTNLLVKNLQSDVSTQSLRPVFSSNTLPRTRVVEDGTGEQPTLIPIAEPDLSNGNGTGSGIGNGNKLKKPVLPELSPCCSKDSLVTAMQNLGFNDLPSTAQHLVLNDVMTQMSKSSDLIFSTLPVPALGTHEDHDASLQYVEDLDIWLEGLPPCMLINSQTMTVTTAL

Target ORF information:

RefSeq Version	NM_001178583.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Vhc1p (VHC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178583.3

ORF Insert Sequence:

ATGGTTAGTA GGTTTTATCA GATTCCAGGT ACGCATCGGC CATCGTCGGC AATATCTTCT 
TCAAATGAAT CTTCTTCACT GTTGTCTGCA AGGAGAATAA GTCAAACATA TTTCAATTAT 
CAGGCGACCC CTGAATGCCA AAAAGTTTCC TCTAAGTATG ATCCTGATAA CCCAAACAAA 
GATAAGTTGG GAACATACGA TGGGGTATTT GTGCCTACTG CTTTAAACGT ATTGTCTATC 
CTTATGTTTC TTCGTTTTGG CTTCATTTTG GGTCAGTTAG GTATTATATG CACCATCGGT 
CTTCTGCTAT TGAGTTACAC TATTAATCTT CTCACCACGT TAAGTATATC TGCTATATCT 
ACAAACGGGA CTGTTAGGGG CGGGGGTGCT TATTATATGA TTTCAAGAAG TTTAGGACCT 
GAATTTGGTG GATCCATTGG GCTTGTCTTT TTCTTAGGGC AGGTGTTCAA TGCAGGTATG 
AACGCAGTGG GTATTATCGA ACCTTTACTC TATAACTTGG GTTATTCTGC TCAAGGCGAG 
CCACCTGCAG CTTTGGGAGA GCTACTACCA AGAGGGCATT GGCACGAATT TACATATGCC 
ACGGTCATTC TTTTCTTATG TTTTTCTGTG GCTTTTGTTG GCTCGCAAAC AGTGTCAAGA 
GCAGGGAATA TTCTTTTTTT GGTACTGGCA GCTTCTATAT TTTCCATTCC ACTTTCTGCA 
TTGATAAGAT CGCCATTCAC TGAAGGCGGT ATCAGTTATA CAGGTCCTTC ATGGCAAACT 
TTTCATGATA ACCTGCTCCC TCACTTAACA AAAGGTGCAG CAGGTTCGTT ACTCAAAGGT 
AAGGAAACAT TCAATGATTT ATTTGGCGTT TTTTTTCCTG CTACAGCTGG TATATTCGCG 
GGCGCAGGAA TGTCAAGTGA ATTGAGAAAG CCCTCTAAAT CAATTCCTAA GGGTACCTTA 
TGGGGTTTAC TTTTCACTTT TATCTGTTAT GCCGTTGTTG TCTTCTCCAT GGGTTGTTCC 
ATTCCCAGAA GATCGTTATA TGATGAAGTA CAAATTATAC AGACGATCAG TTCCGTTCAG 
TGGGTTATAT TCATGGGTGA AATGGCTACT TCTCTTTTCT CCATCATAGT GGGGATGCTC 
GGTGCTGCTT ATGTATTAGA AGCAATTGCA AAGGATAACA TTATTCCTGG TTTGGAGATT 
TTTGCTCATT CACCGTTATA TTCATTGATT TTTACTTGGA TTCTAACACA GCTATGTTTG 
TTTTCAGACG TCAATAAAAT CGCCACATTT ATCACAATGA CATTCTTAAT GACGTTTGTG 
GTCATGAATT TGGCGTGCTT CCTATTAGGT ATTTCCTCTG CTCCCAATTT CAGGCCCTCT 
TTCAAATACT TTAACAGGTA TACTACAGCA ATTGGCGCAT TACTTTCTGT TGTTGCGATG 
TTAATAGTTG ATGGCATCTC AGCGTCTGTC TTGTTTTTGG CTATGATTTT GCTATTTTTA 
TTCATTCATT ATTTTTCACC ACCGAAGTCT TGGGGTGATG TGTCTCAAAG TTTAATATAT 
CATCAAGTGA GAAAGTATTT GCTTCGCCTA CGTCAAGACA ATATTAAATA CTGGAGGCCT 
CAAATATTGC TGTTTGTAGA TAATCCAAGG ACGAGCTGGA ACTTAATAAG GTTTTGCAAT 
CATTTGAAAA AAGGTGGTTT GTATATTTTA GGCCATGTTG CAGTAACTGC GGATTTTCCG 
AAGCAACTAA ATGAATTAAA AACTCAGCAG AAAGCTTGGA TGAAGATCAG AGACATGGCA 
GCAATAAAGG CTTTTGTCCA AGTTGGGACT GGTCCCTCCC TTATATGGGG AATTAGGAAT 
GTTTTTATTG GTTCCGGGCT TGGAGGTATG AAACCAAACA TTACTGTTGT CGGTTTTTTT 
GACCTCGAAA GTTATAGAAA GCACATCCCA CAAAGTCGCA GCCAAAATAA CCTTCAGAAA 
CAGGTGGAAA TAAAAGCTAC AGTGCCACGT AGTACATGCT CTGATGTCAA AATCAATGTT 
CCATTGCCTA CGGATGAGTG TAAAAATGAA ACCAAAGTTA ATGTACAACA ATGGGTTCAA 
ATTGTAGAAG ATCTATCACT GATGCAATCC AATATTGCCA TAGCGCATGG ATTCAAAAAT 
TTAGAGATAC CGAACAAAAG GGACAGCTGC TTTCCAAAAA AAACTATAGA TCTTTATCCC 
ATCCAAATGT GCGGAAAAGT TGAGGCAAAG GGTGACCAGC CTGCGGCTAT CACTACTAAC 
TTTGATACGT ATACACTGAT TCTACAATTA GCTGCCATTT TGGTTACTGT TCCTGAATGG 
AAGCGCACAC ATTCGCTTCG AGTTATTTTA TTTGTGGAAC AAGAATATCA TAGAACGAAC 
GAAACTCAAC GAATGAAAAA GCTATTACAG GTTCTAAGAA TTGACGCAGA GGTTTTGGTA 
GTATCTTTAG ATCAATTCAG AGTATACAAC ACAATTGTCA AGGGAGACCC AATCGTGTTT 
GATTATGTCA ACTCGAAATT GGCCGATAAT GAATGGTGGA AAGATTTGGT TGAAGCTCGT 
GATACATTAA AACCGAAGCG GAGGTTTTCC ACTATTGAAC CTCAAACGAT AGCAAAGCAA 
TTTACACAAT CAAGAAAATA TACGTCTGGG GTTCAGAAAT TGGGTGTCTC ATTTACAATG 
AATACGAGAA TGCCAACTAA CCGCATTGAT ACCCCATGCG AGAGTGAGGA CTCCGATTTG 
GATACAGATC TTACGTCAAT TCGTGATGCC TTCTCGGCAT CGACTAATAT TTCAGTAGGT 
AAGGACTTAA CGACCAAATC GAAGACCGGT TCCGACAGAA CAAATTTACT CGTAAAGAAT 
TTACAAAGCG ATGTGTCAAC ACAGTCGTTA AGACCCGTTT TCTCGAGTAA TACTTTGCCG 
AGGACGAGAG TCGTGGAAGA TGGCACGGGT GAACAGCCGA CACTGATCCC AATTGCCGAA 
CCTGACCTTT CGAATGGGAA CGGTACTGGG AGCGGTATTG GCAACGGCAA CAAATTAAAG 
AAACCGGTTC TTCCCGAATT ATCACCTTGT TGTTCAAAAG ATAGTTTAGT CACAGCGATG 
CAAAATTTAG GTTTCAATGA TCTTCCCAGC ACTGCTCAAC ATTTGGTTCT GAATGATGTG 
ATGACACAAA TGTCAAAGAG TTCGGATTTA ATTTTTTCAA CCTTGCCAGT TCCAGCTCTT 
GGAACACATG AAGATCACGA TGCAAGTTTA CAATATGTCG AGGACTTGGA TATTTGGTTG 
GAGGGCTTAC CTCCATGCAT GTTAATCAAT TCGCAAACCA TGACAGTAAC TACTGCATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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VHC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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