Gene Symbol | IST2 |
Entrez Gene ID | 852382 |
Full Name | Ist2p |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » IST2 cDNA ORF clone
Gene Symbol | IST2 |
Entrez Gene ID | 852382 |
Full Name | Ist2p |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001178434.1 | NP_009643.2 | Ist2p |
Kluyveromyces lactis | KLLA0B12287g | XP_452077.1 |
Saccharomyces cerevisiae (baker's yeast) | IST2 | NP_009643.2 |
Eremothecium gossypii | AGOS_ADL300W | NP_983796.1 |
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The authors show that, in BY4741 background, the lack of Ist2 results in the accumulation of higher amounts of sodium when the cells are exposed to the presence of this cation, demonstrating the importance of Ist2 for the maintenance of low intracellular levels of toxic sodium.
Title: Lack of cortical endoplasmic reticulum protein Ist2 alters sodium accumulation in Saccharomyces cerevisiae cells.
IST2 localization depends on the presence of a PM-binding domain, an intrinsically disordered linker region of sufficient length.
Title: Intrinsically disordered linker and plasma membrane-binding motif sort Ist2 and Ssy1 to junctions.
Ist2 in the yeast cortical endoplasmic reticulum promotes trafficking of the amino acid transporter Bap2 to the plasma membrane.
Title: Ist2 in the yeast cortical endoplasmic reticulum promotes trafficking of the amino acid transporter Bap2 to the plasma membrane.
Data show that binding of phosphatidylinositol phosphates leads to efficient accumulation of Ist2 at domains of the cortical ER from where the protein may reach the PM independently of the function of the sec-pathway.
Title: Binding of plasma membrane lipids recruits the yeast integral membrane protein Ist2 to the cortical ER.
Results suggest that a direct interaction of the Ist2 sorting signal with lipids at the plasma membrane places Ist2 at contact sites between cortical ER and plasma membrane.
Title: A signal comprising a basic cluster and an amphipathic alpha-helix interacts with lipids and is required for the transport of Ist2 to the yeast cortical ER.
The following IST2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IST2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi01795 | |
Clone ID Related Accession (Same CDS sequence) | NM_001178434.1 | |
Accession Version | NM_001178434.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | Ist2p | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGTCGCAGA CAATTACATC TCTAGATCCG AATTGTGTTA TTGTATTCAA TAAAACTTCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_009643.2 |
CDS | 1..2841 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001178434.1 |
1 | ATGTCGCAGA CAATTACATC TCTAGATCCG AATTGTGTTA TTGTATTCAA TAAAACTTCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Life with 6000 genes. |
Complete DNA sequence of yeast chromosome II. |