APN2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	APN2
Entrez Gene ID	852262
Full Name	DNA-(apurinic or apyrimidinic site) lyase APN2
Synonyms	ETH1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178259.1	NP_009534.1	DNA-(apurinic or apyrimidinic site) lyase APN2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	APN2	NP_009534.1
Kluyveromyces lactis	KLLA0E10473g	XP_454423.1
Eremothecium gossypii	AGOS_AFR016W	NP_985563.1
Rattus norvegicus (Norway rat)	Apex2l1	NP_001264325.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Direct physical evidence that Apn2 not only repair cellular base damage but also prevent break accumulation that can result from abasic sites being channeled into other base excision repair pathway(s).
Title: Apn1 and Apn2 endonucleases prevent accumulation of repair-associated DNA breaks in budding yeast as revealed by direct chromosomal analysis.

The following APN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01691	NM_001178259.1 Latest version!	Saccharomyces cerevisiae S288C DNA-(apurinic or apyrimidinic site) lyase APN2 (APN2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APN2 ( NM_001178259.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009534.1 Saccharomyces cerevisiae S288C DNA-(apurinic or apyrimidinic site) lyase APN2 (APN2), partial mRNA.

Price & Availability↑

CloneID	OSi01691
Clone ID Related Accession (Same CDS sequence)	NM_001178259.1
Accession Version	NM_001178259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA-(apurinic or apyrimidinic site) lyase APN2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCATCAA GCGAAAACAC GTTACTGGAT GGAAAGTCAG AGAACACAAT ACGATTTTTA 
ACTTTCAATG TCAATGGTAT AAGAACCTTT TTTCATTATC AACCATTTTC TCAAATGAAT 
CAATCCCTTA GATCTGTTTT CGACTTTTTT CGAGCAGACA TAATAACATT CCAAGAGCTC 
AAGACGGAAA AATTGTCTAT CTCTAAGTGG GGGAGAGTTG ATGGTTTTTA TTCTTTTATT 
TCTATCCCTC AAACCAGAAA GGGATATTCT GGCGTTGGCT GCTGGATTAG AATTCCGGAA 
AAGAACCACC CACTATACCA TGCATTACAA GTCGTTAAGG CAGAAGAAGG TATAACGGGT 
TACTTGACAA TAAAAAATGG TAAGCATTCA GCAATCTCCT ATAGAAACGA CGTAAATCAA 
GGAATTGGTG GTTACGATTC TTTAGATCCC GATTTAGATG AGAAAAGTGC ACTGGAACTA 
GATTCAGAAG GCAGATGTGT TATGGTTGAA CTGGCATGTG GAATAGTTAT TATCAGTGTA 
TATTGTCCCG CAAATTCGAA CTCATCGGAG GAGGGTGAGA TGTTTAGATT AAGGTTCTTG 
AAAGTTTTAT TAAGAAGAGT TCGGAATTTG GACAAAATTG GGAAGAAGAT TGTGCTAATG 
GGCGACGTAA ATGTTTGCCG GGATCTTATA GACAGTGCCG ATACATTAGA ACAATTCTCA 
ATTCCAATAA CAGATCCCAT GGGTGGAACA AAGTTAGAAG CACAATATAG GGATAAAGCA 
ATCCAATTTA TTATCAATCC GGACACGCCA CATCGGAGGA TATTTAATCA AATATTGGCT 
GATTCACTTT TACCAGACGC GAGTAAAAGG GGGATACTGA TAGACACTAC GAGGCTAATT 
CAAACAAGAA ATCGACTTAA AATGTATACA GTCTGGAATA TGTTAAAAAA TTTAAGACCT 
TCGAATTATG GCTCACGGAT AGATTTTATC CTAGTGTCCT TAAAGCTTGA ACGATGCATA 
AAAGCAGCTG ACATTCTTCC GGATATATTG GGCTCTGACC ATTGTCCTGT GTATTCTGAT 
TTAGATATAC TGGACGACAG AATTGAACCT GGTACGACAC AAGTTCCCAT ACCAAAATTC 
GAAGCAAGGT ACAAATATAA TTTAAGAAAC CATAATGTTT TAGAGATGTT TGCCAAAAAG 
GATACGAATA AAGAATCTAA TAAACAAAAA TATTGTGTAT CAAAAGTCAT GAATACCAAA 
AAAAACAGCA ACATCAAAAA CAAATCGCTC GACTCATTTT TCCAGAAGGT AAATGGAGAA 
AAAGATGACA GGATTAAAGA ATCCTCTGAA ATTCCACAGC AAGCTAAAAA AAGAATCTCC 
ACGCCAAAGT TGAATTTCAA GGATGTCTTT GGAAAGCCTC CCCTGTGCAG GCATGGGGAG 
GAATCCATGC TGAAAACATC GAAAACTTCG GCCAATCCAG GTAGAAAGTT CTGGATTTGC 
AAGAGATCTC GGGGTGATTC AAATAATACA GAATCATCTT GTGGGTTTTT TCAGTGGGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009534.1
CDS	1..1563
Translation	MSSSENTLLDGKSENTIRFLTFNVNGIRTFFHYQPFSQMNQSLRSVFDFFRADIITFQELKTEKLSISKWGRVDGFYSFISIPQTRKGYSGVGCWIRIPEKNHPLYHALQVVKAEEGITGYLTIKNGKHSAISYRNDVNQGIGGYDSLDPDLDEKSALELDSEGRCVMVELACGIVIISVYCPANSNSSEEGEMFRLRFLKVLLRRVRNLDKIGKKIVLMGDVNVCRDLIDSADTLEQFSIPITDPMGGTKLEAQYRDKAIQFIINPDTPHRRIFNQILADSLLPDASKRGILIDTTRLIQTRNRLKMYTVWNMLKNLRPSNYGSRIDFILVSLKLERCIKAADILPDILGSDHCPVYSDLDILDDRIEPGTTQVPIPKFEARYKYNLRNHNVLEMFAKKDTNKESNKQKYCVSKVMNTKKNSNIKNKSLDSFFQKVNGEKDDRIKESSEIPQQAKKRISTPKLNFKDVFGKPPLCRHGEESMLKTSKTSANPGRKFWICKRSRGDSNNTESSCGFFQWV

Target ORF information:

RefSeq Version	NM_001178259.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA-(apurinic or apyrimidinic site) lyase APN2 (APN2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178259.1

ORF Insert Sequence:

ATGTCATCAA GCGAAAACAC GTTACTGGAT GGAAAGTCAG AGAACACAAT ACGATTTTTA 
ACTTTCAATG TCAATGGTAT AAGAACCTTT TTTCATTATC AACCATTTTC TCAAATGAAT 
CAATCCCTTA GATCTGTTTT CGACTTTTTT CGAGCAGACA TAATAACATT CCAAGAGCTC 
AAGACGGAAA AATTGTCTAT CTCTAAGTGG GGGAGAGTTG ATGGTTTTTA TTCTTTTATT 
TCTATCCCTC AAACCAGAAA GGGATATTCT GGCGTTGGCT GCTGGATTAG AATTCCGGAA 
AAGAACCACC CACTATACCA TGCATTACAA GTCGTTAAGG CAGAAGAAGG TATAACGGGT 
TACTTGACAA TAAAAAATGG TAAGCATTCA GCAATCTCCT ATAGAAACGA CGTAAATCAA 
GGAATTGGTG GTTACGATTC TTTAGATCCC GATTTAGATG AGAAAAGTGC ACTGGAACTA 
GATTCAGAAG GCAGATGTGT TATGGTTGAA CTGGCATGTG GAATAGTTAT TATCAGTGTA 
TATTGTCCCG CAAATTCGAA CTCATCGGAG GAGGGTGAGA TGTTTAGATT AAGGTTCTTG 
AAAGTTTTAT TAAGAAGAGT TCGGAATTTG GACAAAATTG GGAAGAAGAT TGTGCTAATG 
GGCGACGTAA ATGTTTGCCG GGATCTTATA GACAGTGCCG ATACATTAGA ACAATTCTCA 
ATTCCAATAA CAGATCCCAT GGGTGGAACA AAGTTAGAAG CACAATATAG GGATAAAGCA 
ATCCAATTTA TTATCAATCC GGACACGCCA CATCGGAGGA TATTTAATCA AATATTGGCT 
GATTCACTTT TACCAGACGC GAGTAAAAGG GGGATACTGA TAGACACTAC GAGGCTAATT 
CAAACAAGAA ATCGACTTAA AATGTATACA GTCTGGAATA TGTTAAAAAA TTTAAGACCT 
TCGAATTATG GCTCACGGAT AGATTTTATC CTAGTGTCCT TAAAGCTTGA ACGATGCATA 
AAAGCAGCTG ACATTCTTCC GGATATATTG GGCTCTGACC ATTGTCCTGT GTATTCTGAT 
TTAGATATAC TGGACGACAG AATTGAACCT GGTACGACAC AAGTTCCCAT ACCAAAATTC 
GAAGCAAGGT ACAAATATAA TTTAAGAAAC CATAATGTTT TAGAGATGTT TGCCAAAAAG 
GATACGAATA AAGAATCTAA TAAACAAAAA TATTGTGTAT CAAAAGTCAT GAATACCAAA 
AAAAACAGCA ACATCAAAAA CAAATCGCTC GACTCATTTT TCCAGAAGGT AAATGGAGAA 
AAAGATGACA GGATTAAAGA ATCCTCTGAA ATTCCACAGC AAGCTAAAAA AAGAATCTCC 
ACGCCAAAGT TGAATTTCAA GGATGTCTTT GGAAAGCCTC CCCTGTGCAG GCATGGGGAG 
GAATCCATGC TGAAAACATC GAAAACTTCG GCCAATCCAG GTAGAAAGTT CTGGATTTGC 
AAGAGATCTC GGGGTGATTC AAATAATACA GAATCATCTT GTGGGTTTTT TCAGTGGGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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APN2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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