ORF » Species Summary » Saccharomyces cerevisiae S288C » NCL1 cDNA ORF clone

NCL1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following NCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01686 NM_001178264.1
Latest version!		 Saccharomyces cerevisiae S288C tRNA (cytosine-C5-)-methyltransferase (NCL1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NCL1 ( NM_001178264.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009529.1 Saccharomyces cerevisiae S288C tRNA (cytosine-C5-)-methyltransferase (NCL1), partial mRNA.
    Price & Availability↑
    CloneID OSi01686
    Clone ID Related Accession (Same CDS sequence) NM_001178264.1
    Accession Version NM_001178264.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product tRNA (cytosine-C5-)-methyltransferase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCTAGAA GAAAGAATTT CAAAAAAGGG AACAAGAAGA CTTTTGGTGC TCGTGATGAC 
    TCGAGAGCTC AAAAAAACTG GTCTGAACTG GTAAAGGAAA ATGAAAAATG GGAAAAATAC 
    TATAAGACTT TAGCTCTTTT CCCAGAAGAT CAATGGGAAG AATTTAAAAA GACATGTCAA 
    GCTCCACTTC CTCTAACTTT TAGAATTACA GGTTCTAGAA AGCATGCCGG TGAGGTCCTG 
    AATTTGTTTA AAGAAAGACA TCTACCAAAC TTGACTAATG TTGAGTTTGA AGGTGAGAAG 
    ATTAAGGCCC CTGTAGAATT ACCTTGGTAT CCAGACCATC TTGCTTGGCA ATTGGACGTT 
    CCTAAGACGG TTATTAGAAA GAATGAACAA TTCGCAAAAA CTCAGAGATT TTTAGTTGTT 
    GAAAATGCCG TTGGTAATAT CTCAAGACAA GAAGCCGTTT CAATGATTCC TCCAATCGTT 
    CTAGAAGTAA AACCTCATCA CACTGTTTTA GATATGTGTG CTGCTCCTGG CTCCAAAACT 
    GCTCAATTAA TCGAAGCCTT GCACAAGGAT ACAGATGAAC CATCTGGTTT CGTTGTAGCT 
    AATGATGCCG ATGCCAGAAG ATCTCATATG TTGGTTCACC AATTGAAGAG ATTGAACAGT 
    GCCAACTTGA TGGTTGTCAA CCATGACGCC CAATTCTTCC CACGTATCAG ATTACATGGC 
    AACTCAAATA ACAAGAATGA TGTTTTAAAA TTTGACAGAA TCCTGTGTGA CGTTCCATGT 
    TCTGGTGATG GTACCATGAG GAAAAATGTT AATGTTTGGA AAGACTGGAA CACACAAGCA 
    GGTCTTGGTT TGCATGCTGT TCAGCTGAAT ATATTAAACA GGGGTTTGCA TCTTCTAAAG 
    AACAACGGTA GATTGGTTTA CTCAACCTGT TCTTTAAATC CTATTGAAAA TGAAGCGGTT 
    GTTGCCGAAG CGTTAAGAAA GTGGGGTGAC AAGATTAGAT TAGTTAACTG TGATGATAAG 
    CTTCCTGGCC TAATAAGATC CAAGGGTGTA TCCAAATGGC CTGTCTATGA CAGAAATTTG 
    ACTGAGAAAA CCAAAGGAGA CGAAGGTACA CTAGATAGTT TCTTTTCACC ATCTGAAGAA 
    GAGGCATCGA AATTCAATTT ACAAAATTGT ATGAGGGTTT ATCCTCACCA ACAAAACACA 
    GGCGGATTTT TCATTACTGT TTTCGAAAAA GTCGAAGATA GCACTGAGGC GGCTACAGAG 
    AAACTATCTT CTGAAACCCC AGCTCTAGAG TCTGAAGGAC CTCAAACAAA GAAAATAAAG 
    GTAGAAGAAG TCCAAAAGAA AGAAAGACTA CCACGTGACG CGAACGAAGA GCCTTTTGTT 
    TTCGTTGATC CACAGCACGA AGCTTTAAAA GTTTGTTGGG ATTTCTACGG CATCGATAAT 
    ATTTTCGACA GAAACACTTG TTTAGTGCGT AACGCCACTG GTGAACCAAC AAGAGTGGTT 
    TACACTGTGT GTCCAGCATT GAAGGATGTT ATTCAAGCGA ATGACGATAG GTTGAAGATT 
    ATTTATTCTG GTGTAAAATT GTTTGTCTCT CAAAGAAGTG ATATCGAATG TTCATGGAGA 
    ATCCAAAGTG AATCATTGCC AATAATGAAA CACCATATGA AATCTAATAG AATTGTTGAA 
    GCTAATTTAG AGATGTTAAA ACACTTGTTA ATCGAATCTT TCCCTAACTT TGACGACATT 
    CGTTCGAAGA ACATCGATAA TGATTTTGTT GAAAAGATGA CAAAATTAAG CTCTGGTTGC 
    GCCTTTATTG ATGTGTCAAG AAATGACCCT GCCAAAGAAA ACTTATTCTT GCCTGTGTGG 
    AAAGGCAACA AGTGTATCAA TTTGATGGTT TGTAAAGAAG ATACTCATGA GCTATTATAT 
    AGGATCTTTG GTATTGATGC GAATGCCAAG GCTACTCCAA GCGCTGAAGA AAAAGAAAAA 
    GAAAAAGAAA CGACTGAATC TCCCGCAGAA ACTACTACCG GAACCTCTAC TGAAGCTCCT 
    AGCGCTGCTA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009529.1
    CDS1..2055
    Translation

    Target ORF information:

    RefSeq Version NM_001178264.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C tRNA (cytosine-C5-)-methyltransferase (NCL1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178264.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCTAGAA GAAAGAATTT CAAAAAAGGG AACAAGAAGA CTTTTGGTGC TCGTGATGAC 
    TCGAGAGCTC AAAAAAACTG GTCTGAACTG GTAAAGGAAA ATGAAAAATG GGAAAAATAC 
    TATAAGACTT TAGCTCTTTT CCCAGAAGAT CAATGGGAAG AATTTAAAAA GACATGTCAA 
    GCTCCACTTC CTCTAACTTT TAGAATTACA GGTTCTAGAA AGCATGCCGG TGAGGTCCTG 
    AATTTGTTTA AAGAAAGACA TCTACCAAAC TTGACTAATG TTGAGTTTGA AGGTGAGAAG 
    ATTAAGGCCC CTGTAGAATT ACCTTGGTAT CCAGACCATC TTGCTTGGCA ATTGGACGTT 
    CCTAAGACGG TTATTAGAAA GAATGAACAA TTCGCAAAAA CTCAGAGATT TTTAGTTGTT 
    GAAAATGCCG TTGGTAATAT CTCAAGACAA GAAGCCGTTT CAATGATTCC TCCAATCGTT 
    CTAGAAGTAA AACCTCATCA CACTGTTTTA GATATGTGTG CTGCTCCTGG CTCCAAAACT 
    GCTCAATTAA TCGAAGCCTT GCACAAGGAT ACAGATGAAC CATCTGGTTT CGTTGTAGCT 
    AATGATGCCG ATGCCAGAAG ATCTCATATG TTGGTTCACC AATTGAAGAG ATTGAACAGT 
    GCCAACTTGA TGGTTGTCAA CCATGACGCC CAATTCTTCC CACGTATCAG ATTACATGGC 
    AACTCAAATA ACAAGAATGA TGTTTTAAAA TTTGACAGAA TCCTGTGTGA CGTTCCATGT 
    TCTGGTGATG GTACCATGAG GAAAAATGTT AATGTTTGGA AAGACTGGAA CACACAAGCA 
    GGTCTTGGTT TGCATGCTGT TCAGCTGAAT ATATTAAACA GGGGTTTGCA TCTTCTAAAG 
    AACAACGGTA GATTGGTTTA CTCAACCTGT TCTTTAAATC CTATTGAAAA TGAAGCGGTT 
    GTTGCCGAAG CGTTAAGAAA GTGGGGTGAC AAGATTAGAT TAGTTAACTG TGATGATAAG 
    CTTCCTGGCC TAATAAGATC CAAGGGTGTA TCCAAATGGC CTGTCTATGA CAGAAATTTG 
    ACTGAGAAAA CCAAAGGAGA CGAAGGTACA CTAGATAGTT TCTTTTCACC ATCTGAAGAA 
    GAGGCATCGA AATTCAATTT ACAAAATTGT ATGAGGGTTT ATCCTCACCA ACAAAACACA 
    GGCGGATTTT TCATTACTGT TTTCGAAAAA GTCGAAGATA GCACTGAGGC GGCTACAGAG 
    AAACTATCTT CTGAAACCCC AGCTCTAGAG TCTGAAGGAC CTCAAACAAA GAAAATAAAG 
    GTAGAAGAAG TCCAAAAGAA AGAAAGACTA CCACGTGACG CGAACGAAGA GCCTTTTGTT 
    TTCGTTGATC CACAGCACGA AGCTTTAAAA GTTTGTTGGG ATTTCTACGG CATCGATAAT 
    ATTTTCGACA GAAACACTTG TTTAGTGCGT AACGCCACTG GTGAACCAAC AAGAGTGGTT 
    TACACTGTGT GTCCAGCATT GAAGGATGTT ATTCAAGCGA ATGACGATAG GTTGAAGATT 
    ATTTATTCTG GTGTAAAATT GTTTGTCTCT CAAAGAAGTG ATATCGAATG TTCATGGAGA 
    ATCCAAAGTG AATCATTGCC AATAATGAAA CACCATATGA AATCTAATAG AATTGTTGAA 
    GCTAATTTAG AGATGTTAAA ACACTTGTTA ATCGAATCTT TCCCTAACTT TGACGACATT 
    CGTTCGAAGA ACATCGATAA TGATTTTGTT GAAAAGATGA CAAAATTAAG CTCTGGTTGC 
    GCCTTTATTG ATGTGTCAAG AAATGACCCT GCCAAAGAAA ACTTATTCTT GCCTGTGTGG 
    AAAGGCAACA AGTGTATCAA TTTGATGGTT TGTAAAGAAG ATACTCATGA GCTATTATAT 
    AGGATCTTTG GTATTGATGC GAATGCCAAG GCTACTCCAA GCGCTGAAGA AAAAGAAAAA 
    GAAAAAGAAA CGACTGAATC TCCCGCAGAA ACTACTACCG GAACCTCTAC TGAAGCTCCT 
    AGCGCTGCTA ATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete DNA sequence of yeast chromosome II.
    The EMBO journal13(24)5795-809(1994 Dec)
    Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K


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