SEC17 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SEC17
Entrez Gene ID	852230
Full Name	Sec17p
Synonyms	RNS3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178290.1	NP_009503.1	Sec17p

Pathways from BioSystems

Homologies

	NAPA	XP_001113230.1
Bos taurus (cattle)	NAPA	NP_001179416.1
Rattus norvegicus (Norway rat)	Napa	NP_542152.1
Danio rerio (zebrafish)	napab	NP_956060.1
Drosophila melanogaster (fruit fly)	Snap	NP_524180.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003192	XP_312889.2
Magnaporthe oryzae (rice blast fungus)	MGG_01121	XP_003717813.1
Caenorhabditis elegans (roundworm)	snap-1	NP_505099.2
Eremothecium gossypii	AGOS_ABR155C	NP_983103.1
Schizosaccharomyces pombe (fission yeast)	sec17	NP_594169.1
Neurospora crassa	NCU01674	XP_956880.2
Saccharomyces cerevisiae (baker's yeast)	SEC17	NP_009503.1
Kluyveromyces lactis	KLLA0E05919g	XP_454213.1
Xenopus tropicalis (tropical clawed frog)	napa	NP_001011280.1
Homo sapiens (human)	NAPA	NP_003818.2
Pan troglodytes (chimpanzee)	NAPA	NP_001233558.1
Canis lupus familiaris (dog)	NAPA	XP_852154.1
Mus musculus (house mouse)	Napa	NP_080174.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sec17 binds to trans-SNARE complexes, oligomerizes, and inserts apolar loops into the apposed membranes, locally disturbing the lipid bilayer and lowering the energy barrier for fusion without Sec18
Title: Sec17 can trigger fusion of trans-SNARE paired membranes without Sec18.

Using a reconstituted proteoliposome fusion system, the authors show that trans-SNARE complex, like cis-SNARE complex, is sensitive to Sec17p/Sec18p mediated disassembly.
Title: HOPS prevents the disassembly of trans-SNARE complexes by Sec17p/Sec18p during membrane fusion.

Data show that the ternary synergy of phosphoinositides and 2 SNARE chaperone systems (SNARE Vam7p and Sec17p/Sec18p/ATP
Title: Phosphoinositides and SNARE chaperones synergistically assemble and remodel SNARE complexes for membrane fusion.

The following SEC17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEC17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01660	NM_001178290.1 Latest version!	Saccharomyces cerevisiae S288C Sec17p (SEC17), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEC17 ( NM_001178290.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009503.1 Saccharomyces cerevisiae S288C Sec17p (SEC17), partial mRNA.

Price & Availability↑

CloneID	OSi01660
Clone ID Related Accession (Same CDS sequence)	NM_001178290.1
Accession Version	NM_001178290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Sec17p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAGACC CTGTAGAGTT ATTGAAAAGA GCTGAGAAGA AGGGTGTTCC TTCATCGGGT 
TTCATGAAAT TGTTTAGCGG TTCTGATTCA TACAAGTTTG AGGAGGCTGC TGATCTTTGT 
GTCCAAGCAG CCACCATTTA CCGTCTAAGA AAAGAGTTAA ACTTGGCAGG AGACTCGTTT 
TTGAAAGCTG CTGACTATCA GAAAAAGGCT GGTAATGAAG ACGAAGCAGG AAATACCTAC 
GTAGAGGCTT ATAAATGCTT TAAAAGCGGT GGAAACTCTG TGAACGCCGT GGATTCATTA 
GAAAATGCTA TTCAAATTTT TACTCATAGG GGGCAGTTCC GGAGAGGTGC TAATTTCAAG 
TTTGAGCTTG GAGAAATTCT AGAAAATGAT TTGCATGACT ATGCAAAAGC TATAGATTGC 
TATGAGCTCG CTGGTGAGTG GTATGCCCAA GACCAGTCGG TAGCATTATC GAATAAGTGT 
TTTATCAAAT GCGCAGATCT AAAGGCTCTT GACGGTCAAT ATATTGAAGC AAGTGATATA 
TATTCGAAGT TGATCAAGAG CAGCATGGGC AATAGATTGA GCCAATGGAG TTTGAAGGAT 
TACTTCCTAA AAAAAGGGCT TTGTCAGCTA GCTGCTACTG ATGCAGTCGC TGCTGCAAGA 
ACTTTACAAG AGGGTCAAAG TGAAGATCCG AATTTTGCGG ATTCAAGGGA GTCAAATTTC 
TTGAAAAGTT TAATCGATGC TGTTAATGAA GGTGATAGCG AGCAGCTAAG CGAACACTGT 
AAGGAGTTTG ACAATTTTAT GAGACTAGAT AAATGGAAAA TTACCATTTT GAATAAAATT 
AAGGAGTCCA TCCAGCAACA AGAAGATGAT TTGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009503.1
CDS	1..879
Translation	MSDPVELLKRAEKKGVPSSGFMKLFSGSDSYKFEEAADLCVQAATIYRLRKELNLAGDSFLKAADYQKKAGNEDEAGNTYVEAYKCFKSGGNSVNAVDSLENAIQIFTHRGQFRRGANFKFELGEILENDLHDYAKAIDCYELAGEWYAQDQSVALSNKCFIKCADLKALDGQYIEASDIYSKLIKSSMGNRLSQWSLKDYFLKKGLCQLAATDAVAAARTLQEGQSEDPNFADSRESNFLKSLIDAVNEGDSEQLSEHCKEFDNFMRLDKWKITILNKIKESIQQQEDDLL

Target ORF information:

RefSeq Version	NM_001178290.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Sec17p (SEC17), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178290.1

ORF Insert Sequence:

ATGTCAGACC CTGTAGAGTT ATTGAAAAGA GCTGAGAAGA AGGGTGTTCC TTCATCGGGT 
TTCATGAAAT TGTTTAGCGG TTCTGATTCA TACAAGTTTG AGGAGGCTGC TGATCTTTGT 
GTCCAAGCAG CCACCATTTA CCGTCTAAGA AAAGAGTTAA ACTTGGCAGG AGACTCGTTT 
TTGAAAGCTG CTGACTATCA GAAAAAGGCT GGTAATGAAG ACGAAGCAGG AAATACCTAC 
GTAGAGGCTT ATAAATGCTT TAAAAGCGGT GGAAACTCTG TGAACGCCGT GGATTCATTA 
GAAAATGCTA TTCAAATTTT TACTCATAGG GGGCAGTTCC GGAGAGGTGC TAATTTCAAG 
TTTGAGCTTG GAGAAATTCT AGAAAATGAT TTGCATGACT ATGCAAAAGC TATAGATTGC 
TATGAGCTCG CTGGTGAGTG GTATGCCCAA GACCAGTCGG TAGCATTATC GAATAAGTGT 
TTTATCAAAT GCGCAGATCT AAAGGCTCTT GACGGTCAAT ATATTGAAGC AAGTGATATA 
TATTCGAAGT TGATCAAGAG CAGCATGGGC AATAGATTGA GCCAATGGAG TTTGAAGGAT 
TACTTCCTAA AAAAAGGGCT TTGTCAGCTA GCTGCTACTG ATGCAGTCGC TGCTGCAAGA 
ACTTTACAAG AGGGTCAAAG TGAAGATCCG AATTTTGCGG ATTCAAGGGA GTCAAATTTC 
TTGAAAAGTT TAATCGATGC TGTTAATGAA GGTGATAGCG AGCAGCTAAG CGAACACTGT 
AAGGAGTTTG ACAATTTTAT GAGACTAGAT AAATGGAAAA TTACCATTTT GAATAAAATT 
AAGGAGTCCA TCCAGCAACA AGAAGATGAT TTGTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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SEC17 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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