ORF » Species Summary » Saccharomyces cerevisiae S288C » SEF1 cDNA ORF clone

SEF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following SEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi01646 NM_001178306.1
Latest version!		 Saccharomyces cerevisiae S288C Sef1p (SEF1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SEF1 ( NM_001178306.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009487.2 Saccharomyces cerevisiae S288C Sef1p (SEF1), partial mRNA.
    Price & Availability↑
    CloneID OSi01646
    Clone ID Related Accession (Same CDS sequence) NM_001178306.1
    Accession Version NM_001178306.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product Sef1p
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTGAAGG ATAATCGAGA TTCTGACCAA GACCAAGATT TTAGTTCTGC TCACATGAAA 
    AGACAACCGG AGCAGCAACA GTTGCAACAG CACCAGTTCC CAAGTAAGAA ACAACGAATA 
    TCTCACCATG ATGACAGTCA TCAAATCAAC CATAGACCAG TTACCTCATG TACACATTGT 
    AGACAGCACA AAATCAAATG CGATGCTAGT CAAAATTTCC CTCATCCTTG CTCCAGATGC 
    GAAAAAATTG GTCTCCACTG TGAAATCAAT CCTCAATTCA GGCCTAAGAA GGGCTCACAG 
    TTGCAACTAC TGAGACAAGA TGTGGATGAA ATCAAATCTA AACTCGATAC TCTTCTGGCC 
    AATGACAGCG TTTTCGTTCA TCTTTTACAA CAGATTCCCA TGGGCAATAG CCTTTTGAAT 
    AAGCTCAATC TGCATCCAAC TCCAACTCCG GGTACTATTA TCCCTAACCC AGATTCTTCT 
    CCTTCCTCAG GTTCTCCAAC TTCTTCCGCG GCTCAACGAG ATTCTAAGGT TTCAGTTCAA 
    ACTTATTTGT CCAGGGAACC CCAACTCTTA CAAGCAAATC AGGGCAGCAA TACGAATAAA 
    TTTAAAGCAA ATAATGAAGC ATCTTCTCAC ATGACGTTGC GCGCATCTTC TTTAGCGCAA 
    GATTCGAAAG GCTTGGTTGC AACAGAGCCA AATAAGCTGC CCCCGCTGCT AAATGACTCA 
    GCATTGCCTA ATAATTCAAA AGAATCTTTA CCTCCTGCTT TGCAAATGGC TTTTTATAAG 
    AACAACTCTG CAGGTAACAC TCCGAACGGC CCCTTCTCTC CAATTCAAAA AACATATTCC 
    CCTCATACTA CGTCGACCAC CGTTACAACG ACAACAAATC AACCACCATT TGCAGCAACA 
    AGCCACGTAG CAACAAATAA CAATGCAGAT AGGACGAAGA CGCCGGTAGT AGCCACCACC 
    ACGACTATGC CATTATTGCC TTCGCCGCAT GCAAATGTAG ATGAGTTTGT ACTGGGCGAT 
    ATTAGTATTT CCATTGAAAA AGCGAATAGA TTACACCATA TTTTCGTGAC TAGGTATCTG 
    CCGTATTTTC CTATTATGTA TTCCAATAAC GCCACCGAAT TATACTCCCA ATCTCAGTTG 
    CTTTTCTGGA CCGTGATGTT GACGGCATGT CTGTCTGATC CTGAACCGAC GATGTATTGC 
    AAGCTAAGCT CTTTGATCAA GCAACTTGCC ATAGAGACCT GCTGGATAAG AACACCTAGA 
    TCCACACATA TTTCGCAAGC TTTGTTAATA TTGTGCATTT GGCCTTTGCC TAACCAAAAA 
    GTCCTAGATG ATTGTTCTTA CCGTTTTGTA GGATTAGCAA AGTCACTGTC TTATCAATTA 
    GGTTTGCACA GAGGTGAATT CATTTCTGAA TTCACAAGAA CTCAAACATC AATGCCAAAT 
    GCAGAAAAGT GGAGAACTAG GACTTGGCTG GGAATATTTT TTGCCGAACT TTGTTGGGCG 
    AGTATCCTTG GTTTGCCACC AACTTCACAG ACAGACTATT TATTAGAAAA AGCCTTATCC 
    TGTGGTGACG AAGAATCAGA AGAAGATAAC AATGACAGTA TTGACAATAA CAACAATGAT 
    AAAAGGAACA AGAAAGACGA GCCGCACGTT GAAAGTAAAT ACAAACTACC GGGCAGTTTT 
    AGAAGATTGC TCAGCCTGGC GAATTTCCAA GCAAAATTGT CTCATATCAT TGGTTCTTCC 
    ACTTCCAGTC CTGATGGTTT ATTGGAACCA AAGTATCGTG CTGAGACACT GTCCATCTTG 
    GGAAAAGAGT TAGATTTATT AGCAAAAACT TTAAATTTCC AGAGTGACGA TACTGTCAAC 
    ATTTATTTTC TTTATGTTAA ATTAACTGTC TGTTGTTTTG CATTCCTACC CGAAACACCT 
    CCTACCGATC AAATTCCATA TGTCACAGAG GCCTATCTAA CAGCTACTAA AATTGTCACT 
    CTATTGAATA ATCTTTTAGA AACACACCAA TTAATTGAAC TGCCTATTTA TATTAGACAA 
    GCTGCTACAT TTTCTGCACT GATTCTCTTT AAATTGCAGT TGACTCCTTT ACTTCCTGAC 
    AAATATTTTG ATTCAGCAAG GCAATCCGTG GTCACTATCC ATAGACTTTA TAGAAATCAG 
    TTAACTGCGT GGGCCACTAG TGTTGAGAAT GATATTTCGA GAACTGCAAG TATGTTAGAA 
    AAACTGAACT TCGTACTGAT CATGCATCCA GAAGTTTTTG TGGAAGAAGA CGGTATTATT 
    TCTAGGATGA GATCACATTT AACAGGGTCT CTATTCTATG ATTTGGTTTG GTGTGTTCAC 
    GAGGCGAGAA GAAGGGAAAT GGATCCCGAA TATAACAAGC AAGCCTTAGA GAAAGCCGCT 
    AAGAAAAGAA AATTTTCCTC AAATGGTATC TACAATGGCA CTTCGTCTAC GGGTGGCATA 
    ACGGACAGAA AACTATATCC ATTGCCACTA TATAACCATA TCTCCAGAGA TGACTTTGAA 
    ACTGTAACAA AAACAACACC AAGTGGAACC ACTGTTACCA CTTTAGTTCC TACTAAGAAT 
    GCCTTAAAGC AGGCAGAAAA GCTAGCCAAG ACAAATAACG GAGATTCTGA CGGTTCTATA 
    ATGGAGATTA ACGGGATACC TCTTTCCATG CTCGGGGAAA CAGGCAGCGT AAAATTTCAA 
    AGTTTATTCG CTAATACCTC GAATAGTAAC GATTATAATA ATAATAGGAC GTTATTGGAT 
    GCGTCTAATG ACATATCAAT TCCCTCTAAT TCAATTTATC CAGTGGCTTC TGTCCCCGCT 
    TCGAATAACA ATCCACAAAG TACTAAGGTA GACTATTATA GTAACGGACC TAGTGTAATT 
    CCTGATCTCT CCATGAAAAG ATCAGTAAGC ACTCCCGTTA ATCATTTTCC TGCGTCCGTT 
    CCAGGGTTAA GGAACCACCC CGTTGGCAAC TTATCTAATA ATGTTACATT GGGAATAGAC 
    CACCCTATTC CAAGGGAGCA CAGTAATTTA CAAAATGTCA CCATGAATTA TAATAATCAA 
    TTCAGCAACG CCAACGCGAT TGGAAGATCA CAAAGTAGTA TGTCCCATTC ACGTACACCG 
    ATTGCTTCGA AGTCAAATAA TATGACAGAT TTGCATTCCG TCGTTTCCGA CCCCGGCTCC 
    TCTAAAAGCA CGGCATATCC ACCTCTGAGC TTGTTTTCCA AAAGTAATGA TATCAATAGC 
    AACAAAACGA ACCAACGATT TTCTACGGGC ACTAACACTG TAACTTCTTC TAACTTCCAA 
    ACCATCGATA ATGAAAACAA CGTGAAGACT CCTGGAAACA AACTAACGGA TTTTTTTCAG 
    CAACAAAGTG CAGGCTGGAT TGAAGGCAAC TCCAGTAATG ATGACTTTTT TGGTTGGTTC 
    GATATGAACA TGGAACAAGG TTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_009487.2
    CDS1..3447
    Translation

    Target ORF information:

    RefSeq Version NM_001178306.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C Sef1p (SEF1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001178306.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGTGAAGG ATAATCGAGA TTCTGACCAA GACCAAGATT TTAGTTCTGC TCACATGAAA 
    AGACAACCGG AGCAGCAACA GTTGCAACAG CACCAGTTCC CAAGTAAGAA ACAACGAATA 
    TCTCACCATG ATGACAGTCA TCAAATCAAC CATAGACCAG TTACCTCATG TACACATTGT 
    AGACAGCACA AAATCAAATG CGATGCTAGT CAAAATTTCC CTCATCCTTG CTCCAGATGC 
    GAAAAAATTG GTCTCCACTG TGAAATCAAT CCTCAATTCA GGCCTAAGAA GGGCTCACAG 
    TTGCAACTAC TGAGACAAGA TGTGGATGAA ATCAAATCTA AACTCGATAC TCTTCTGGCC 
    AATGACAGCG TTTTCGTTCA TCTTTTACAA CAGATTCCCA TGGGCAATAG CCTTTTGAAT 
    AAGCTCAATC TGCATCCAAC TCCAACTCCG GGTACTATTA TCCCTAACCC AGATTCTTCT 
    CCTTCCTCAG GTTCTCCAAC TTCTTCCGCG GCTCAACGAG ATTCTAAGGT TTCAGTTCAA 
    ACTTATTTGT CCAGGGAACC CCAACTCTTA CAAGCAAATC AGGGCAGCAA TACGAATAAA 
    TTTAAAGCAA ATAATGAAGC ATCTTCTCAC ATGACGTTGC GCGCATCTTC TTTAGCGCAA 
    GATTCGAAAG GCTTGGTTGC AACAGAGCCA AATAAGCTGC CCCCGCTGCT AAATGACTCA 
    GCATTGCCTA ATAATTCAAA AGAATCTTTA CCTCCTGCTT TGCAAATGGC TTTTTATAAG 
    AACAACTCTG CAGGTAACAC TCCGAACGGC CCCTTCTCTC CAATTCAAAA AACATATTCC 
    CCTCATACTA CGTCGACCAC CGTTACAACG ACAACAAATC AACCACCATT TGCAGCAACA 
    AGCCACGTAG CAACAAATAA CAATGCAGAT AGGACGAAGA CGCCGGTAGT AGCCACCACC 
    ACGACTATGC CATTATTGCC TTCGCCGCAT GCAAATGTAG ATGAGTTTGT ACTGGGCGAT 
    ATTAGTATTT CCATTGAAAA AGCGAATAGA TTACACCATA TTTTCGTGAC TAGGTATCTG 
    CCGTATTTTC CTATTATGTA TTCCAATAAC GCCACCGAAT TATACTCCCA ATCTCAGTTG 
    CTTTTCTGGA CCGTGATGTT GACGGCATGT CTGTCTGATC CTGAACCGAC GATGTATTGC 
    AAGCTAAGCT CTTTGATCAA GCAACTTGCC ATAGAGACCT GCTGGATAAG AACACCTAGA 
    TCCACACATA TTTCGCAAGC TTTGTTAATA TTGTGCATTT GGCCTTTGCC TAACCAAAAA 
    GTCCTAGATG ATTGTTCTTA CCGTTTTGTA GGATTAGCAA AGTCACTGTC TTATCAATTA 
    GGTTTGCACA GAGGTGAATT CATTTCTGAA TTCACAAGAA CTCAAACATC AATGCCAAAT 
    GCAGAAAAGT GGAGAACTAG GACTTGGCTG GGAATATTTT TTGCCGAACT TTGTTGGGCG 
    AGTATCCTTG GTTTGCCACC AACTTCACAG ACAGACTATT TATTAGAAAA AGCCTTATCC 
    TGTGGTGACG AAGAATCAGA AGAAGATAAC AATGACAGTA TTGACAATAA CAACAATGAT 
    AAAAGGAACA AGAAAGACGA GCCGCACGTT GAAAGTAAAT ACAAACTACC GGGCAGTTTT 
    AGAAGATTGC TCAGCCTGGC GAATTTCCAA GCAAAATTGT CTCATATCAT TGGTTCTTCC 
    ACTTCCAGTC CTGATGGTTT ATTGGAACCA AAGTATCGTG CTGAGACACT GTCCATCTTG 
    GGAAAAGAGT TAGATTTATT AGCAAAAACT TTAAATTTCC AGAGTGACGA TACTGTCAAC 
    ATTTATTTTC TTTATGTTAA ATTAACTGTC TGTTGTTTTG CATTCCTACC CGAAACACCT 
    CCTACCGATC AAATTCCATA TGTCACAGAG GCCTATCTAA CAGCTACTAA AATTGTCACT 
    CTATTGAATA ATCTTTTAGA AACACACCAA TTAATTGAAC TGCCTATTTA TATTAGACAA 
    GCTGCTACAT TTTCTGCACT GATTCTCTTT AAATTGCAGT TGACTCCTTT ACTTCCTGAC 
    AAATATTTTG ATTCAGCAAG GCAATCCGTG GTCACTATCC ATAGACTTTA TAGAAATCAG 
    TTAACTGCGT GGGCCACTAG TGTTGAGAAT GATATTTCGA GAACTGCAAG TATGTTAGAA 
    AAACTGAACT TCGTACTGAT CATGCATCCA GAAGTTTTTG TGGAAGAAGA CGGTATTATT 
    TCTAGGATGA GATCACATTT AACAGGGTCT CTATTCTATG ATTTGGTTTG GTGTGTTCAC 
    GAGGCGAGAA GAAGGGAAAT GGATCCCGAA TATAACAAGC AAGCCTTAGA GAAAGCCGCT 
    AAGAAAAGAA AATTTTCCTC AAATGGTATC TACAATGGCA CTTCGTCTAC GGGTGGCATA 
    ACGGACAGAA AACTATATCC ATTGCCACTA TATAACCATA TCTCCAGAGA TGACTTTGAA 
    ACTGTAACAA AAACAACACC AAGTGGAACC ACTGTTACCA CTTTAGTTCC TACTAAGAAT 
    GCCTTAAAGC AGGCAGAAAA GCTAGCCAAG ACAAATAACG GAGATTCTGA CGGTTCTATA 
    ATGGAGATTA ACGGGATACC TCTTTCCATG CTCGGGGAAA CAGGCAGCGT AAAATTTCAA 
    AGTTTATTCG CTAATACCTC GAATAGTAAC GATTATAATA ATAATAGGAC GTTATTGGAT 
    GCGTCTAATG ACATATCAAT TCCCTCTAAT TCAATTTATC CAGTGGCTTC TGTCCCCGCT 
    TCGAATAACA ATCCACAAAG TACTAAGGTA GACTATTATA GTAACGGACC TAGTGTAATT 
    CCTGATCTCT CCATGAAAAG ATCAGTAAGC ACTCCCGTTA ATCATTTTCC TGCGTCCGTT 
    CCAGGGTTAA GGAACCACCC CGTTGGCAAC TTATCTAATA ATGTTACATT GGGAATAGAC 
    CACCCTATTC CAAGGGAGCA CAGTAATTTA CAAAATGTCA CCATGAATTA TAATAATCAA 
    TTCAGCAACG CCAACGCGAT TGGAAGATCA CAAAGTAGTA TGTCCCATTC ACGTACACCG 
    ATTGCTTCGA AGTCAAATAA TATGACAGAT TTGCATTCCG TCGTTTCCGA CCCCGGCTCC 
    TCTAAAAGCA CGGCATATCC ACCTCTGAGC TTGTTTTCCA AAAGTAATGA TATCAATAGC 
    AACAAAACGA ACCAACGATT TTCTACGGGC ACTAACACTG TAACTTCTTC TAACTTCCAA 
    ACCATCGATA ATGAAAACAA CGTGAAGACT CCTGGAAACA AACTAACGGA TTTTTTTCAG 
    CAACAAAGTG CAGGCTGGAT TGAAGGCAAC TCCAGTAATG ATGACTTTTT TGGTTGGTTC 
    GATATGAACA TGGAACAAGG TTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete DNA sequence of yeast chromosome II.
    The EMBO journal13(24)5795-809(1994 Dec)
    Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K


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