CDC27 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC27
Entrez Gene ID	852194
Full Name	anaphase promoting complex subunit CDC27
Synonyms	APC3,SNB1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178324.2	NP_009469.2	anaphase promoting complex subunit CDC27

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E20857g	XP_454893.1
Saccharomyces cerevisiae (baker's yeast)	CDC27	NP_009469.2
Eremothecium gossypii	AGOS_AGL294W	NP_986373.1
Schizosaccharomyces pombe (fission yeast)	nuc2	NP_594604.2
Magnaporthe oryzae (rice blast fungus)	MGG_17195	XP_003717296.1
Neurospora crassa	NCU00213	XP_956598.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CDC27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01632	NM_001178324.2 Latest version!	Saccharomyces cerevisiae S288C anaphase promoting complex subunit CDC27 (CDC27), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC27 ( NM_001178324.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009469.2 Saccharomyces cerevisiae S288C anaphase promoting complex subunit CDC27 (CDC27), partial mRNA.

Price & Availability↑

CloneID	OSi01632
Clone ID Related Accession (Same CDS sequence)	NM_001178324.2
Accession Version	NM_001178324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	anaphase promoting complex subunit CDC27
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178324.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCGGTAA ATCCTGAGTT AGCACCGTTC ACCCTCTCGA GAGGAATCCC CAGCTTTGAT 
GATCAAGCTT TGAGCACCAT CATACAGCTT CAGGACTGCA TTCAGCAGGC TATTCAGCAG 
TTGAACTACA GTACCGCAGA GTTTTTGGCC GAACTGCTCT ATGCTGAATG CTCCATTCTC 
GATAAATCAA GTGTTTACTG GTCCGATGCG GTATATTTAT ATGCACTTTC GCTGTTTCTG 
AATAAAAGCT ACCACACTGC GTTCCAGATA TCCAAAGAAT TCAAGGAGTA TCATCTTGGT 
ATCGCTTACA TATTCGGGCG TTGTGCTTTA CAGCTTTCGC AGGGAGTTAA CGAAGCTATC 
CTCACTCTTC TCTCAATAAT AAATGTATTT TCTTCGAACA GCAGTAATAC GCGCATAAAT 
ATGGTGTTGA ATTCTAATCT CGTCCATATT CCCGATTTGG CCACTTTGAA TTGCCTGTTA 
GGCAATCTTT ATATGAAACT GGACCATTCT AAGGAGGGAG CATTTTATCA TTCTGAAGCA 
TTAGCCATTA ATCCTTACCT TTGGGAATCT TACGAAGCGA TTTGTAAAAT GAGAGCTACC 
GTGGATCTCA AGAGGGTTTT TTTCGACATT GCAGGGAAAA AAAGTAATAG TCATAATAAT 
AATGCAGCTT CATCGTTTCC GTCTACATCA CTATCGCATT TCGAACCTCG TTCACAACCT 
AGCTTATATT CAAAAACAAA CAAAAACGGC AATAATAATA TCAATAATAA TGTTAATACA 
CTGTTTCAGT CGTCTAATTC TCCCCCTTCT ACATCGGCAT CTTCTTTTTC TTCCATTCAG 
CATTTCTCAA GGTCACAGCA GCAACAAGCG AACACATCAA TAAGGACCTG CCAGAACAAA 
AATACTCAAA CTCCTAAAAA CCCTGCAATC AACAGTAAGA CGTCTTCTGC GCTACCAAAT 
AACATTTCCA TGAACTTAGT GTCTCCATCC TCCAAACAGC CTACAATAAG CTCGTTGGCC 
AAAGTTTATA ACAGAAACAA ACTTTTAACG ACTCCTCCAT CGAAACTGTT AAATAACGAT 
AGGAACCACC AAAATAACAA TAATAATAAT AATAATAATA ATAATAATAA TAATAATAAT 
AATAATAATA ATAATAATAA TAACATTATA AATAAAACAA CTTTCAAAAC TCCAAGAAAC 
CTATATTCCT CAACAGGAAG GTTAACAACT TCCAAGAAAA ATCCAAGGTC TTTAATAATC 
AGTAACTCAA TACTAACGAG TGATTATTCA ATTACGCTGC CTGAAATCAT GTATAATTTC 
GCTTTAATAT TAAGGTCGTC ATCACAATAC AATTCGTTCA AGGCAATAAG ACTGTTCGAG 
TCTCAAATCC CATCTCATAT TAAAGACACA ATGCCATGGT GTCTAGTGCA ATTAGGAAAA 
CTTCATTTTG AGATCATTAA TTATGATATG TCCTTAAAGT ATTTCAATAG ATTGAAAGAC 
CTACAACCGG CAAGGGTAAA AGATATGGAA ATTTTTTCTA CTTTGCTGTG GCATTTGCAT 
GACAAGGTTA AATCTTCAAA TTTGGCAAAT GGGCTAATGG ATACAATGCC TAATAAGCCC 
GAAACATGGT GTTGTATAGG TAATTTGCTA TCATTGCAAA AGGATCATGA TGCCGCAATA 
AAAGCCTTCG AAAAAGCTAC TCAGTTAGAC CCAAATTTTG CATACGCGTA TACTTTGCAA 
GGTCATGAAC ATTCTTCCAA CGATTCTTCG GATTCTGCCA AGACATGCTA TAGAAAGGCG 
CTAGCTTGTG ATCCTCAGCA TTACAATGCA TATTACGGAT TGGGTACGAG CGCTATGAAA 
TTAGGTCAAT ATGAAGAAGC GTTGTTATAT TTTGAAAAGG CAAGGTCAAT TAATCCCGTC 
AATGTTGTGT TAATCTGTTG TTGCGGTGGT TCTTTAGAAA AGCTGGGCTA TAAGGAAAAG 
GCTCTACAAT ATTATGAACT AGCATGTCAT TTGCAACCGA CTTCCTCGCT ATCCAAATAT 
AAGATGGGCC AGTTGCTCTA TTCCATGACA AGATATAATG TTGCTTTGCA AACTTTTGAA 
GAATTGGTGA AACTCGTTCC TGATGATGCC ACAGCCCATT ATTTGCTGGG TCAAACATAT 
AGAATAGTTG GGAGGAAAAA AGATGCAATC AAGGAGCTAA CTGTTGCTAT GAATTTGGAT 
CCAAAGGGTA ACCAAGTTAT CATCGATGAA TTACAAAAAT GTCATATGCA AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009469.2
CDS	1..2277
Translation	MAVNPELAPFTLSRGIPSFDDQALSTIIQLQDCIQQAIQQLNYSTAEFLAELLYAECSILDKSSVYWSDAVYLYALSLFLNKSYHTAFQISKEFKEYHLGIAYIFGRCALQLSQGVNEAILTLLSIINVFSSNSSNTRINMVLNSNLVHIPDLATLNCLLGNLYMKLDHSKEGAFYHSEALAINPYLWESYEAICKMRATVDLKRVFFDIAGKKSNSHNNNAASSFPSTSLSHFEPRSQPSLYSKTNKNGNNNINNNVNTLFQSSNSPPSTSASSFSSIQHFSRSQQQQANTSIRTCQNKNTQTPKNPAINSKTSSALPNNISMNLVSPSSKQPTISSLAKVYNRNKLLTTPPSKLLNNDRNHQNNNNNNNNNNNNNNNNNNNNNNNNIINKTTFKTPRNLYSSTGRLTTSKKNPRSLIISNSILTSDYSITLPEIMYNFALILRSSSQYNSFKAIRLFESQIPSHIKDTMPWCLVQLGKLHFEIINYDMSLKYFNRLKDLQPARVKDMEIFSTLLWHLHDKVKSSNLANGLMDTMPNKPETWCCIGNLLSLQKDHDAAIKAFEKATQLDPNFAYAYTLQGHEHSSNDSSDSAKTCYRKALACDPQHYNAYYGLGTSAMKLGQYEEALLYFEKARSINPVNVVLICCCGGSLEKLGYKEKALQYYELACHLQPTSSLSKYKMGQLLYSMTRYNVALQTFEELVKLVPDDATAHYLLGQTYRIVGRKKDAIKELTVAMNLDPKGNQVIIDELQKCHMQE

Target ORF information:

RefSeq Version	NM_001178324.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C anaphase promoting complex subunit CDC27 (CDC27), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178324.2

ORF Insert Sequence:

ATGGCGGTAA ATCCTGAGTT AGCACCGTTC ACCCTCTCGA GAGGAATCCC CAGCTTTGAT 
GATCAAGCTT TGAGCACCAT CATACAGCTT CAGGACTGCA TTCAGCAGGC TATTCAGCAG 
TTGAACTACA GTACCGCAGA GTTTTTGGCC GAACTGCTCT ATGCTGAATG CTCCATTCTC 
GATAAATCAA GTGTTTACTG GTCCGATGCG GTATATTTAT ATGCACTTTC GCTGTTTCTG 
AATAAAAGCT ACCACACTGC GTTCCAGATA TCCAAAGAAT TCAAGGAGTA TCATCTTGGT 
ATCGCTTACA TATTCGGGCG TTGTGCTTTA CAGCTTTCGC AGGGAGTTAA CGAAGCTATC 
CTCACTCTTC TCTCAATAAT AAATGTATTT TCTTCGAACA GCAGTAATAC GCGCATAAAT 
ATGGTGTTGA ATTCTAATCT CGTCCATATT CCCGATTTGG CCACTTTGAA TTGCCTGTTA 
GGCAATCTTT ATATGAAACT GGACCATTCT AAGGAGGGAG CATTTTATCA TTCTGAAGCA 
TTAGCCATTA ATCCTTACCT TTGGGAATCT TACGAAGCGA TTTGTAAAAT GAGAGCTACC 
GTGGATCTCA AGAGGGTTTT TTTCGACATT GCAGGGAAAA AAAGTAATAG TCATAATAAT 
AATGCAGCTT CATCGTTTCC GTCTACATCA CTATCGCATT TCGAACCTCG TTCACAACCT 
AGCTTATATT CAAAAACAAA CAAAAACGGC AATAATAATA TCAATAATAA TGTTAATACA 
CTGTTTCAGT CGTCTAATTC TCCCCCTTCT ACATCGGCAT CTTCTTTTTC TTCCATTCAG 
CATTTCTCAA GGTCACAGCA GCAACAAGCG AACACATCAA TAAGGACCTG CCAGAACAAA 
AATACTCAAA CTCCTAAAAA CCCTGCAATC AACAGTAAGA CGTCTTCTGC GCTACCAAAT 
AACATTTCCA TGAACTTAGT GTCTCCATCC TCCAAACAGC CTACAATAAG CTCGTTGGCC 
AAAGTTTATA ACAGAAACAA ACTTTTAACG ACTCCTCCAT CGAAACTGTT AAATAACGAT 
AGGAACCACC AAAATAACAA TAATAATAAT AATAATAATA ATAATAATAA TAATAATAAT 
AATAATAATA ATAATAATAA TAACATTATA AATAAAACAA CTTTCAAAAC TCCAAGAAAC 
CTATATTCCT CAACAGGAAG GTTAACAACT TCCAAGAAAA ATCCAAGGTC TTTAATAATC 
AGTAACTCAA TACTAACGAG TGATTATTCA ATTACGCTGC CTGAAATCAT GTATAATTTC 
GCTTTAATAT TAAGGTCGTC ATCACAATAC AATTCGTTCA AGGCAATAAG ACTGTTCGAG 
TCTCAAATCC CATCTCATAT TAAAGACACA ATGCCATGGT GTCTAGTGCA ATTAGGAAAA 
CTTCATTTTG AGATCATTAA TTATGATATG TCCTTAAAGT ATTTCAATAG ATTGAAAGAC 
CTACAACCGG CAAGGGTAAA AGATATGGAA ATTTTTTCTA CTTTGCTGTG GCATTTGCAT 
GACAAGGTTA AATCTTCAAA TTTGGCAAAT GGGCTAATGG ATACAATGCC TAATAAGCCC 
GAAACATGGT GTTGTATAGG TAATTTGCTA TCATTGCAAA AGGATCATGA TGCCGCAATA 
AAAGCCTTCG AAAAAGCTAC TCAGTTAGAC CCAAATTTTG CATACGCGTA TACTTTGCAA 
GGTCATGAAC ATTCTTCCAA CGATTCTTCG GATTCTGCCA AGACATGCTA TAGAAAGGCG 
CTAGCTTGTG ATCCTCAGCA TTACAATGCA TATTACGGAT TGGGTACGAG CGCTATGAAA 
TTAGGTCAAT ATGAAGAAGC GTTGTTATAT TTTGAAAAGG CAAGGTCAAT TAATCCCGTC 
AATGTTGTGT TAATCTGTTG TTGCGGTGGT TCTTTAGAAA AGCTGGGCTA TAAGGAAAAG 
GCTCTACAAT ATTATGAACT AGCATGTCAT TTGCAACCGA CTTCCTCGCT ATCCAAATAT 
AAGATGGGCC AGTTGCTCTA TTCCATGACA AGATATAATG TTGCTTTGCA AACTTTTGAA 
GAATTGGTGA AACTCGTTCC TGATGATGCC ACAGCCCATT ATTTGCTGGG TCAAACATAT 
AGAATAGTTG GGAGGAAAAA AGATGCAATC AAGGAGCTAA CTGTTGCTAT GAATTTGGAT 
CCAAAGGGTA ACCAAGTTAT CATCGATGAA TTACAAAAAT GTCATATGCA AGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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CDC27 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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