PKC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	PKC1
Entrez Gene ID	852169
Full Name	protein kinase C
Synonyms	CLY15,CLY5,CLY7,HPO2,STT1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178345.2	NP_009445.2	protein kinase C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PRKCE	XP_851861.1
Drosophila melanogaster (fruit fly)	Pkc98E	NP_524545.2
Schizosaccharomyces pombe (fission yeast)	pck2	NP_595950.1
Homo sapiens (human)	PRKCE	NP_005391.1
Danio rerio (zebrafish)	prkcea	XP_005156351.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002748	XP_312175.5
Rattus norvegicus (Norway rat)	Prkce	NP_058867.1
Danio rerio (zebrafish)	prkceb	XP_002664164.2
Saccharomyces cerevisiae (baker's yeast)	PKC1	NP_009445.2
Eremothecium gossypii	AGOS_ACR191C	NP_983593.1
Xenopus tropicalis (tropical clawed frog)	prkce	NP_001107724.1
Bos taurus (cattle)	PRKCE	NP_001104590.1
Gallus gallus (chicken)	PRKCE	XP_419464.3
Caenorhabditis elegans (roundworm)	pkc-1	NP_001256395.1
Kluyveromyces lactis	KLLA0E06447g	XP_454238.1
Magnaporthe oryzae (rice blast fungus)	MGG_08689	XP_003719292.1
Neurospora crassa	NCU06544	XP_962251.1
Pan troglodytes (chimpanzee)	PRKCE	XP_003309040.1
	PRKCE	XP_001112763.1
Mus musculus (house mouse)	Prkce	NP_035234.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Pkc1-mediated degradation of Pah1 was attenuated in the cho1Delta mutant, which is deficient in PS synthase, supporting the notion that the phospholipid regulates Pkc1 activity in vivo.
Title: Phosphorylation of lipid metabolic enzymes by yeast protein kinase C requires phosphatidylserine and diacylglycerol.

Constitutively active Pkc1 can drive cells through a mitotic checkpoint arrest, which suggests that Pkc1-dependent activation of PP2A(Cdc55) plays a critical role in checkpoint signaling.
Title: Protein Kinase C Controls Binding of Igo/ENSA Proteins to Protein Phosphatase 2A in Budding Yeast.

Pkc1 also enhances H3T45 phosphorylation and this modification is required for H3K56 acetylation.
Title: Protein kinase C coordinates histone H3 phosphorylation and acetylation.

Authors propose that Rho1 and Pkc1 coordinate septin ring assembly with membrane and cell wall remodeling partly by controlling Syp1 residence at the bud neck.
Title: Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly.

This study connects the Pkc1 protein to the maintenance of genome integrity in response to genotoxic stresses.
Title: Protein kinase C controls activation of the DNA integrity checkpoint.

The following PKC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01611	NM_001178345.2 Latest version!	Saccharomyces cerevisiae S288C protein kinase C (PKC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PKC1 ( NM_001178345.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009445.2 Saccharomyces cerevisiae S288C protein kinase C (PKC1), partial mRNA.

Price & Availability↑

CloneID	OSi01611
Clone ID Related Accession (Same CDS sequence)	NM_001178345.2
Accession Version	NM_001178345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Apr 26, 2011 this sequence version replaced NM_001178345.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGTTTTT CACAATTGGA GCAGAACATT AAAAAAAAGA TAGCCGTCGA AGAAAATATT 
ATTCGGGGAG CTTCTGCCCT CAAGAAAAAG ACTAGCAATG TTATGGTCAT TCAGAAATGT 
AATACGAATA TTAGAGAGGC ACGTCAAAAT CTTGAGTACT TGGAGGATAG CTTAAAAAAA 
TTACGGTTGA AGACCGCTCA ACAAAGTCAG GGTGAAAATG GCAGCGAAGA TAATGAGCGA 
TGCAATTCGA AGGAATACGG GTTTCTTTCT ACCAAATCGC CAAATGAACA CATATTTTCT 
CGTTTGGATT TAGTAAAATA TGATTGCCCT TCTTTGGCGC AAAGAATCCA ATACATGTTG 
CAACAATTAG AATTCAAATT ACAGGTGGAA AAGCAATACC AAGAAGCCAA TACTAAATTG 
ACTAAATTGT ATCAAATTGA CGGTGATCAA CGTAGTAGTT CTGCGGCAGA AGGGGGTGCT 
ATGGAATCCA AATACAGGAT TCAAATGCTT AACAAAGCTT TGAAGAAATA CCAAGCTATT 
AACGTTGACT TTGATCAATT TAAACACCAA CCCAATGACA TAATGGATAA TCAACAACCG 
AAATTCCGAA GAAAACAACT GACTGGCGTT CTAACAATTG GAATAACTGC TGCTAGAGAT 
GTTGACCATA TACAATCACC GATGTTTGCC AGGAAGCCAG AGAGCTACGT TACTATAAAA 
ATCGATGATA CGATCAAAGC CAGAACGAAG CCTTCTAGAA ATGACAGGTG GAGTGAAGAT 
TTTCAAATTC CAGTTGAAAA AGGGAATGAA ATCGAGATTA CTGTATATGA TAAGGTGAAC 
GACTCGCTCA TTCCAGTGGC TATAATGTGG TTACTGCTTT CCGATATCGC TGAAGAAATT 
CGTAAGAAGA AAGCCGGACA AACAAATGAA CAACAAGGAT GGGTCAACGC TTCAAACATT 
AATGGTGGTT CTTCTCTCGC TAGCGAAGAG GGAAGCACAT TGACTTCTAC ATACTCAAAC 
TCAGCCATTC AGTCTACTTC AGCAAAGAAT GTGCAAGGCG AAAATACATC AACTAGTCAA 
ATCAGTACGA ATTCATGGTT TGTCTTAGAA CCATCCGGGC AGATTTTGTT AACACTAGGA 
TTCCACAAGT CTTCTCAAAT AGAAAGGAAG CAGTTGATGG GTGGACTACA TCGTCATGGT 
GCTATTATCA ATAGGAAAGA AGAGATATTC GAACAGCATG GCCACCACTT TGTACAAAAA 
TCATTTTACA ACATTATGTG CTGTGCATAT TGTGGTGATT TCCTTCGGTA TACTGGGTTC 
CAATGTCAAG ATTGTAAATT TTTATGTCAC AAAAAATGTT ACACCAATGT TGTTACTAAG 
TGTATTGCTA AAACTTCTAC TGATACGGAT CCGGACGAGG CAAAGTTGAA CCACCGTATT 
CCTCATAGAT TCCTGCCTAC TTCAAACCGT GGTACTAAAT GGTGCTGTCA CTGTGGTTAT 
ATTTTACCAT GGGGTAGACA TAAAGTACGC AAATGTTCTG AATGTGGTAT AATGTGTCAT 
GCTCAATGTG CTCATTTGGT TCCCGATTTC TGTGGCATGT CGATGGAAAT GGCTAATAAA 
ATTCTGAAAA CGATTCAAGA CACAAAACGT AATCAAGAGA AAAAGAAGAG GACAGTTCCG 
TCGGCACAGT TGGGGTCATC TATTGGTACT GCCAATGGTA GTGATCTTTC CCCATCCAAA 
TTAGCTGAAA GGGCAAACGC TCCTTTGCCT CCTCAGCCAA GAAAACATGA TAAAACTCCT 
TCGCCTCAGA AGGTAGGAAG AGATTCTCCA ACTAAGCAGC ATGATCCTAT TATTGATAAA 
AGAATTTCAC TACAAACGCA CGGGCGTGAG AAACTAAATA AATTTATCGA TGAAAATGAG 
GCCTATTTAA ATTTTACAGA AGGTGCACAA CAAACCGCCG AATTTTCATC ACCAGAGAAA 
ACACTGGATC CAACGTCGAA TAGACGTTCC TTGGGACTCA CTGATTTATC TATTGAGCAT 
AGTCAAACTT GGGAATCTAA AGATGATTTA ATGAGAGATG AACTTGAACT GTGGAAAGCA 
CAACGTGAGG AAATGGAACT TGAAATAAAA CAAGATAGTG GGGAAATCCA AGAGGATCTA 
GAAGTTGATC ACATTGATTT AGAGACCAAA CAGAAACTAG ACTGGGAGAA CAAAAATGAT 
TTCCGTGAAG CTGATTTGAC AATAGATTCC ACACACACAA ATCCATTCCG CGATATGAAT 
AGTGAAACCT TTCAAATTGA ACAAGACCAT GCTTCAAAGG AAGTACTACA AGAAACAGTG 
TCCTTGGCTC CTACAAGTAC TCATGCCTCC AGAACCACTG ATCAACAATC TCCGCAGAAA 
TCACAAACCA GCACAAGCGC AAAGCATAAA AAGAGAGCGG CTAAACGTCG TAAGGTTTCA 
TTAGATAATT TCGTATTACT CAAAGTTCTT GGTAAAGGTA ATTTTGGTAA AGTTATTTTA 
TCTAAATCAA AGAATACTGA CAGGCTTTGT GCCATAAAAG TTCTGAAGAA AGATAATATT 
ATTCAAAATC ATGACATCGA GAGTGCAAGA GCAGAAAAGA AAGTATTTTT GTTAGCCACA 
AAAACTAAAC ACCCATTCTT AACCAATCTA TACTGCTCTT TTCAAACTGA AAACCGTATA 
TATTTTGCTA TGGAGTTTAT TGGGGGCGGT GACTTAATGT GGCATGTTCA AAACCAAAGA 
CTATCTGTAA GAAGGGCCAA ATTTTATGCC GCCGAGGTCT TACTGGCTCT GAAATATTTT 
CATGATAATG GTGTAATATA CCGTGATTTG AAGTTGGAAA ACATTCTATT AACTCCAGAA 
GGTCATATAA AAATTGCCGA TTATGGTTTG TGTAAAGATG AAATGTGGTA TGGTAATAGA 
ACTTCTACAT TTTGTGGCAC ACCAGAATTT ATGGCTCCAG AAATTTTAAA AGAGCAAGAA 
TATACCAAAG CTGTCGATTG GTGGGCATTT GGGGTGCTGC TATATCAAAT GCTACTGTGC 
CAATCTCCAT TCTCAGGAGA TGACGAAGAT GAAGTTTTTA ACGCTATCCT TACCGATGAA 
CCCTTATACC CAATAGATAT GGCAGGTGAG ATTGTACAAA TATTCCAAGG CCTATTAACA 
AAAGATCCTG AAAAGAGGTT GGGTGCTGGT CCCAGGGACG CAGACGAAGT TATGGAAGAA 
CCTTTCTTCC GTAACATCAA CTTTGACGAT ATCTTAAACC TTCGCGTGAA ACCACCCTAC 
ATCCCAGAAA TTAAATCTCC GGAAGATACA TCATATTTCG AGCAAGAATT CACCTCTGCG 
CCACCCACAC TTACACCTCT GCCCTCTGTT TTGACAACGA GCCAGCAAGA AGAGTTTAGA 
GGATTTTCCT TTATGCCAGA TGATTTGGAT TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009445.2
CDS	1..3456
Translation	MSFSQLEQNIKKKIAVEENIIRGASALKKKTSNVMVIQKCNTNIREARQNLEYLEDSLKKLRLKTAQQSQGENGSEDNERCNSKEYGFLSTKSPNEHIFSRLDLVKYDCPSLAQRIQYMLQQLEFKLQVEKQYQEANTKLTKLYQIDGDQRSSSAAEGGAMESKYRIQMLNKALKKYQAINVDFDQFKHQPNDIMDNQQPKFRRKQLTGVLTIGITAARDVDHIQSPMFARKPESYVTIKIDDTIKARTKPSRNDRWSEDFQIPVEKGNEIEITVYDKVNDSLIPVAIMWLLLSDIAEEIRKKKAGQTNEQQGWVNASNINGGSSLASEEGSTLTSTYSNSAIQSTSAKNVQGENTSTSQISTNSWFVLEPSGQILLTLGFHKSSQIERKQLMGGLHRHGAIINRKEEIFEQHGHHFVQKSFYNIMCCAYCGDFLRYTGFQCQDCKFLCHKKCYTNVVTKCIAKTSTDTDPDEAKLNHRIPHRFLPTSNRGTKWCCHCGYILPWGRHKVRKCSECGIMCHAQCAHLVPDFCGMSMEMANKILKTIQDTKRNQEKKKRTVPSAQLGSSIGTANGSDLSPSKLAERANAPLPPQPRKHDKTPSPQKVGRDSPTKQHDPIIDKRISLQTHGREKLNKFIDENEAYLNFTEGAQQTAEFSSPEKTLDPTSNRRSLGLTDLSIEHSQTWESKDDLMRDELELWKAQREEMELEIKQDSGEIQEDLEVDHIDLETKQKLDWENKNDFREADLTIDSTHTNPFRDMNSETFQIEQDHASKEVLQETVSLAPTSTHASRTTDQQSPQKSQTSTSAKHKKRAAKRRKVSLDNFVLLKVLGKGNFGKVILSKSKNTDRLCAIKVLKKDNIIQNHDIESARAEKKVFLLATKTKHPFLTNLYCSFQTENRIYFAMEFIGGGDLMWHVQNQRLSVRRAKFYAAEVLLALKYFHDNGVIYRDLKLENILLTPEGHIKIADYGLCKDEMWYGNRTSTFCGTPEFMAPEILKEQEYTKAVDWWAFGVLLYQMLLCQSPFSGDDEDEVFNAILTDEPLYPIDMAGEIVQIFQGLLTKDPEKRLGAGPRDADEVMEEPFFRNINFDDILNLRVKPPYIPEIKSPEDTSYFEQEFTSAPPTLTPLPSVLTTSQQEEFRGFSFMPDDLDL

Target ORF information:

RefSeq Version	NM_001178345.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase C (PKC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178345.2

ORF Insert Sequence:

ATGAGTTTTT CACAATTGGA GCAGAACATT AAAAAAAAGA TAGCCGTCGA AGAAAATATT 
ATTCGGGGAG CTTCTGCCCT CAAGAAAAAG ACTAGCAATG TTATGGTCAT TCAGAAATGT 
AATACGAATA TTAGAGAGGC ACGTCAAAAT CTTGAGTACT TGGAGGATAG CTTAAAAAAA 
TTACGGTTGA AGACCGCTCA ACAAAGTCAG GGTGAAAATG GCAGCGAAGA TAATGAGCGA 
TGCAATTCGA AGGAATACGG GTTTCTTTCT ACCAAATCGC CAAATGAACA CATATTTTCT 
CGTTTGGATT TAGTAAAATA TGATTGCCCT TCTTTGGCGC AAAGAATCCA ATACATGTTG 
CAACAATTAG AATTCAAATT ACAGGTGGAA AAGCAATACC AAGAAGCCAA TACTAAATTG 
ACTAAATTGT ATCAAATTGA CGGTGATCAA CGTAGTAGTT CTGCGGCAGA AGGGGGTGCT 
ATGGAATCCA AATACAGGAT TCAAATGCTT AACAAAGCTT TGAAGAAATA CCAAGCTATT 
AACGTTGACT TTGATCAATT TAAACACCAA CCCAATGACA TAATGGATAA TCAACAACCG 
AAATTCCGAA GAAAACAACT GACTGGCGTT CTAACAATTG GAATAACTGC TGCTAGAGAT 
GTTGACCATA TACAATCACC GATGTTTGCC AGGAAGCCAG AGAGCTACGT TACTATAAAA 
ATCGATGATA CGATCAAAGC CAGAACGAAG CCTTCTAGAA ATGACAGGTG GAGTGAAGAT 
TTTCAAATTC CAGTTGAAAA AGGGAATGAA ATCGAGATTA CTGTATATGA TAAGGTGAAC 
GACTCGCTCA TTCCAGTGGC TATAATGTGG TTACTGCTTT CCGATATCGC TGAAGAAATT 
CGTAAGAAGA AAGCCGGACA AACAAATGAA CAACAAGGAT GGGTCAACGC TTCAAACATT 
AATGGTGGTT CTTCTCTCGC TAGCGAAGAG GGAAGCACAT TGACTTCTAC ATACTCAAAC 
TCAGCCATTC AGTCTACTTC AGCAAAGAAT GTGCAAGGCG AAAATACATC AACTAGTCAA 
ATCAGTACGA ATTCATGGTT TGTCTTAGAA CCATCCGGGC AGATTTTGTT AACACTAGGA 
TTCCACAAGT CTTCTCAAAT AGAAAGGAAG CAGTTGATGG GTGGACTACA TCGTCATGGT 
GCTATTATCA ATAGGAAAGA AGAGATATTC GAACAGCATG GCCACCACTT TGTACAAAAA 
TCATTTTACA ACATTATGTG CTGTGCATAT TGTGGTGATT TCCTTCGGTA TACTGGGTTC 
CAATGTCAAG ATTGTAAATT TTTATGTCAC AAAAAATGTT ACACCAATGT TGTTACTAAG 
TGTATTGCTA AAACTTCTAC TGATACGGAT CCGGACGAGG CAAAGTTGAA CCACCGTATT 
CCTCATAGAT TCCTGCCTAC TTCAAACCGT GGTACTAAAT GGTGCTGTCA CTGTGGTTAT 
ATTTTACCAT GGGGTAGACA TAAAGTACGC AAATGTTCTG AATGTGGTAT AATGTGTCAT 
GCTCAATGTG CTCATTTGGT TCCCGATTTC TGTGGCATGT CGATGGAAAT GGCTAATAAA 
ATTCTGAAAA CGATTCAAGA CACAAAACGT AATCAAGAGA AAAAGAAGAG GACAGTTCCG 
TCGGCACAGT TGGGGTCATC TATTGGTACT GCCAATGGTA GTGATCTTTC CCCATCCAAA 
TTAGCTGAAA GGGCAAACGC TCCTTTGCCT CCTCAGCCAA GAAAACATGA TAAAACTCCT 
TCGCCTCAGA AGGTAGGAAG AGATTCTCCA ACTAAGCAGC ATGATCCTAT TATTGATAAA 
AGAATTTCAC TACAAACGCA CGGGCGTGAG AAACTAAATA AATTTATCGA TGAAAATGAG 
GCCTATTTAA ATTTTACAGA AGGTGCACAA CAAACCGCCG AATTTTCATC ACCAGAGAAA 
ACACTGGATC CAACGTCGAA TAGACGTTCC TTGGGACTCA CTGATTTATC TATTGAGCAT 
AGTCAAACTT GGGAATCTAA AGATGATTTA ATGAGAGATG AACTTGAACT GTGGAAAGCA 
CAACGTGAGG AAATGGAACT TGAAATAAAA CAAGATAGTG GGGAAATCCA AGAGGATCTA 
GAAGTTGATC ACATTGATTT AGAGACCAAA CAGAAACTAG ACTGGGAGAA CAAAAATGAT 
TTCCGTGAAG CTGATTTGAC AATAGATTCC ACACACACAA ATCCATTCCG CGATATGAAT 
AGTGAAACCT TTCAAATTGA ACAAGACCAT GCTTCAAAGG AAGTACTACA AGAAACAGTG 
TCCTTGGCTC CTACAAGTAC TCATGCCTCC AGAACCACTG ATCAACAATC TCCGCAGAAA 
TCACAAACCA GCACAAGCGC AAAGCATAAA AAGAGAGCGG CTAAACGTCG TAAGGTTTCA 
TTAGATAATT TCGTATTACT CAAAGTTCTT GGTAAAGGTA ATTTTGGTAA AGTTATTTTA 
TCTAAATCAA AGAATACTGA CAGGCTTTGT GCCATAAAAG TTCTGAAGAA AGATAATATT 
ATTCAAAATC ATGACATCGA GAGTGCAAGA GCAGAAAAGA AAGTATTTTT GTTAGCCACA 
AAAACTAAAC ACCCATTCTT AACCAATCTA TACTGCTCTT TTCAAACTGA AAACCGTATA 
TATTTTGCTA TGGAGTTTAT TGGGGGCGGT GACTTAATGT GGCATGTTCA AAACCAAAGA 
CTATCTGTAA GAAGGGCCAA ATTTTATGCC GCCGAGGTCT TACTGGCTCT GAAATATTTT 
CATGATAATG GTGTAATATA CCGTGATTTG AAGTTGGAAA ACATTCTATT AACTCCAGAA 
GGTCATATAA AAATTGCCGA TTATGGTTTG TGTAAAGATG AAATGTGGTA TGGTAATAGA 
ACTTCTACAT TTTGTGGCAC ACCAGAATTT ATGGCTCCAG AAATTTTAAA AGAGCAAGAA 
TATACCAAAG CTGTCGATTG GTGGGCATTT GGGGTGCTGC TATATCAAAT GCTACTGTGC 
CAATCTCCAT TCTCAGGAGA TGACGAAGAT GAAGTTTTTA ACGCTATCCT TACCGATGAA 
CCCTTATACC CAATAGATAT GGCAGGTGAG ATTGTACAAA TATTCCAAGG CCTATTAACA 
AAAGATCCTG AAAAGAGGTT GGGTGCTGGT CCCAGGGACG CAGACGAAGT TATGGAAGAA 
CCTTTCTTCC GTAACATCAA CTTTGACGAT ATCTTAAACC TTCGCGTGAA ACCACCCTAC 
ATCCCAGAAA TTAAATCTCC GGAAGATACA TCATATTTCG AGCAAGAATT CACCTCTGCG 
CCACCCACAC TTACACCTCT GCCCTCTGTT TTGACAACGA GCCAGCAAGA AGAGTTTAGA 
GGATTTTCCT TTATGCCAGA TGATTTGGAT TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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PKC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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