MSS4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MSS4
Entrez Gene ID	851789
Full Name	1-phosphatidylinositol-4-phosphate 5-kinase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180516.1	NP_010494.1	1-phosphatidylinositol-4-phosphate 5-kinase

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0A04301g	XP_451188.1
Saccharomyces cerevisiae (baker's yeast)	MSS4	NP_010494.1
Oryza sativa (rice)	Os03g0356596	NP_001173425.1
Eremothecium gossypii	AGOS_AGL096W	NP_986571.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The identified 8-amino acid conserved insert in fungal PIP5K plays an important role in the binding of this protein with membrane surface.
Title: Identification of a conserved 8 aa insert in the PIP5K protein in the Saccharomycetaceae family of fungi and the molecular dynamics simulations and structural analysis to investigate its potential functional role.

Data show the PtdIns 4-kinase Pik1 is involved in Atg9 trafficking through the Golgi and is involved in both nonselective and selective types of autophagy, whereas the PtdIns4P 5-kinase Mss4 is involved in mitophagy but not nonselective autophagy.
Title: Phosphatidylinositol 4-kinases are required for autophagic membrane trafficking.

Findings show that the exocytic signal involves PI delivery Mss4 by Sfh5, generation of PI(4,5)P(2), and PI(4,5)P(2)-dependent regulation of Cdc42 and the actin cytoskeleton.
Title: A phosphatidylinositol-transfer protein and phosphatidylinositol-4-phosphate 5-kinase control Cdc42 to regulate the actin cytoskeleton and secretory pathway in yeast.

We characterized a novel multicopy suppressor gene of ISP-1-mediated cell death in yeast, MSS4. MSS4 encodes a phosphatidylinositol-4-phosphate 5-kinase that synthesizes phosphatidylinositol (4,5)-bisphosphate (PI4,5P(2)).
Title: Disturbance of sphingolipid biosynthesis abrogates the signaling of Mss4, phosphatidylinositol-4-phosphate 5-kinase, in yeast.

The following MSS4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSS4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01288	NM_001180516.1 Latest version!	Saccharomyces cerevisiae S288C 1-phosphatidylinositol-4-phosphate 5-kinase (MSS4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSS4 ( NM_001180516.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010494.1 Saccharomyces cerevisiae S288C 1-phosphatidylinositol-4-phosphate 5-kinase (MSS4), partial mRNA.

Price & Availability↑

CloneID	OSi01288
Clone ID Related Accession (Same CDS sequence)	NM_001180516.1
Accession Version	NM_001180516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	1-phosphatidylinositol-4-phosphate 5-kinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAGTCT TGCGATCACA ACCTCCTTCA GTTGTACCGC TACATCTGAC AACATCCACC 
AGTCGCAAAA CAGAACAGGA ACCATCGCTA TTGCACTCTG CAATAATTGA GCGACATCAA 
GACCGTTCGG TGCCGAATTC GAATTCGAAT CCGGATTCGA ACCATCGAAT AAAAAAGGAT 
CGCAATAATC ACACTAGCTA CCATTCTTCA TCGAATTCGG AGTCAAACAT GGAAAGTCCT 
CGCTTGTCAG ATGGTGAGTC TTCCACTCCG ACCTCTATTG AAGAGTTAAA CCCAACAATA 
AATAATTCGA GGCTGGTGAA GAGAAACTAC TCAATATCAA TTGATCCTTT GCATGACAAC 
AGCAATAATA ATACGGATGA TGATCATCCA AACACCATAA CTTCTCCCCG ACCAAATAGC 
ACTAGTAACA AGGAAATGCA GAAATATAGT TTTCCCGAAG GTAAGGAGTC GAAGAAAATA 
ACAACACCCT CGTTAAACTC GAATAATTGT TTGGATTTGG ACAATTCCAG TCTTGTTCAC 
ACAGATTCGT ATATACAAGA CTTAAATGAC GATCATATTT TACTAAACAA GCGCGTTTCG 
AGGCGATCTT CCAGAATATC GGCAGTAACA GCAACTTCCA CGACAATTAA GCAAAGAAGA 
AATACTCAGG ATTCAAACCT GCCCAATATT CCCTTTCACG CTTCCAAGCA TTCTCAAATT 
CTACCTATGG ATGATTCAGA TGTAATAAAA TTGGCCAATG GAGATACCAG CATGAAACCA 
AATTCTGCTA CTAAAATTAG TCACTCAATG ACTTCTTTGC CTCTTCACCC ACTTCCACAA 
CCTTCTCAAA AGTCAAAGCA ATATCATATG ATATCCAAAT CAACCACTTC TTTGCCGCCA 
GAGAATGACC ACTACTATCA ACACAGTCGT GGCACCAACC ATAATCACGC TGCTAATGCT 
GCTGCTGTTA ATAATAATAC TACTACTACT ACCGCTGCTA CGGGTCTGAA AAGATCAGAG 
TCTGCAACGG CAGAAATTAA GAAGATGAGA CAATCTTTGC TGCATAAAAG AGAAATGAAA 
AGGAAAAGAA AAACATTTTT GGTGGATGAC GATCGTGTTT TGATCGGTAA TAAAGTCAGT 
GAAGGCCATG TCAACTTTAT TATAGCTTAT AACATGCTAA CTGGTATTCG TGTCGCAGTG 
TCACGTTGTT CTGGCATAAT GAAACCTTTA ACTCCGGCAG ACTTTAGATT TACGAAGAAG 
CTTGCCTTTG ATTATCACGG AAATGAATTG ACCCCTTCTT CTCAGTATGC ATTTAAGTTT 
AAGGACTACT GTCCCGAAGT CTTTAGGGAA CTTCGTGCAT TATTCGGCTT AGACCCTGCT 
GATTATTTGG TTTCGTTAAC TTCCAAGTAC ATTTTGAGTG AGTTGAACTC GCCAGGTAAA 
AGTGGTTCAT TTTTTTATTA TTCGAGAGAT TACAAATATA TTATCAAGAC CATACATCAT 
TCTGAGCATA TTCATCTGAG AAAGCACATA CAAGAATACT ATAACCATGT AAGAGACAAT 
CCAAACACTT TGATTTGTCA ATTTTATGGT TTGCATAGAG TGAAAATGCC TATATCGTTT 
CAAAATAAAA TTAAGCATCG GAAAATTTAC TTCCTAGTCA TGAATAACTT ATTTCCACCA 
CACTTAGACA TTCACATTAC TTATGATTTA AAAGGTTCCA CATGGGGCCG TTTTACCAAT 
TTGGATAAAG AAAGGTTGGC GAAAGATAGA TCATATAGGC CTGTGATGAA AGATTTAAAT 
TGGCTTGAAG AAGGTCAGAA AATTAAATTC GGTCCATTGA AGAAGAAGAC TTTTTTGACA 
CAGCTGAAAA AAGATGTGGA ATTGCTTGCT AAATTGAATA CAATGGACTA TTCCTTGTTA 
ATTGGCATTC ATGACATCAA TAAAGCTAAA GAAGACGACT TACAATTGGC GGATACGGCA 
TCTATTGAGG AGCAACCACA GACTCAAGGA CCCATAAGAA CCGGTACCGG AACGGTAGTA 
CGACATTTTT TTAGAGAGTT TGAAGGTGGA ATTCGAGCAT CTGATCAATT CAACAATGAT 
GTGGATTTGA TTTATTACGT GGGGATAATC GATTTCTTGA CTAATTACTC AGTTATGAAG 
AAATTAGAAA CGTTTTGGAG AAGTCTACGC CATGATACCA AGTTGGTGAG TGCCATACCT 
CCAAGAGACT ACGCCAATAG GTTTTACGAA TTCATAGAAG ATTCTGTAGA TCCTCTGCCC 
CAGAAAAAAA CTCAATCGTC GTATAGAGAC GACCCTAACC AGAAAAATTA TAAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010494.1
CDS	1..2340
Translation	MSVLRSQPPSVVPLHLTTSTSRKTEQEPSLLHSAIIERHQDRSVPNSNSNPDSNHRIKKDRNNHTSYHSSSNSESNMESPRLSDGESSTPTSIEELNPTINNSRLVKRNYSISIDPLHDNSNNNTDDDHPNTITSPRPNSTSNKEMQKYSFPEGKESKKITTPSLNSNNCLDLDNSSLVHTDSYIQDLNDDHILLNKRVSRRSSRISAVTATSTTIKQRRNTQDSNLPNIPFHASKHSQILPMDDSDVIKLANGDTSMKPNSATKISHSMTSLPLHPLPQPSQKSKQYHMISKSTTSLPPENDHYYQHSRGTNHNHAANAAAVNNNTTTTTAATGLKRSESATAEIKKMRQSLLHKREMKRKRKTFLVDDDRVLIGNKVSEGHVNFIIAYNMLTGIRVAVSRCSGIMKPLTPADFRFTKKLAFDYHGNELTPSSQYAFKFKDYCPEVFRELRALFGLDPADYLVSLTSKYILSELNSPGKSGSFFYYSRDYKYIIKTIHHSEHIHLRKHIQEYYNHVRDNPNTLICQFYGLHRVKMPISFQNKIKHRKIYFLVMNNLFPPHLDIHITYDLKGSTWGRFTNLDKERLAKDRSYRPVMKDLNWLEEGQKIKFGPLKKKTFLTQLKKDVELLAKLNTMDYSLLIGIHDINKAKEDDLQLADTASIEEQPQTQGPIRTGTGTVVRHFFREFEGGIRASDQFNNDVDLIYYVGIIDFLTNYSVMKKLETFWRSLRHDTKLVSAIPPRDYANRFYEFIEDSVDPLPQKKTQSSYRDDPNQKNYKD

Target ORF information:

RefSeq Version	NM_001180516.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C 1-phosphatidylinositol-4-phosphate 5-kinase (MSS4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180516.1

ORF Insert Sequence:

ATGTCAGTCT TGCGATCACA ACCTCCTTCA GTTGTACCGC TACATCTGAC AACATCCACC 
AGTCGCAAAA CAGAACAGGA ACCATCGCTA TTGCACTCTG CAATAATTGA GCGACATCAA 
GACCGTTCGG TGCCGAATTC GAATTCGAAT CCGGATTCGA ACCATCGAAT AAAAAAGGAT 
CGCAATAATC ACACTAGCTA CCATTCTTCA TCGAATTCGG AGTCAAACAT GGAAAGTCCT 
CGCTTGTCAG ATGGTGAGTC TTCCACTCCG ACCTCTATTG AAGAGTTAAA CCCAACAATA 
AATAATTCGA GGCTGGTGAA GAGAAACTAC TCAATATCAA TTGATCCTTT GCATGACAAC 
AGCAATAATA ATACGGATGA TGATCATCCA AACACCATAA CTTCTCCCCG ACCAAATAGC 
ACTAGTAACA AGGAAATGCA GAAATATAGT TTTCCCGAAG GTAAGGAGTC GAAGAAAATA 
ACAACACCCT CGTTAAACTC GAATAATTGT TTGGATTTGG ACAATTCCAG TCTTGTTCAC 
ACAGATTCGT ATATACAAGA CTTAAATGAC GATCATATTT TACTAAACAA GCGCGTTTCG 
AGGCGATCTT CCAGAATATC GGCAGTAACA GCAACTTCCA CGACAATTAA GCAAAGAAGA 
AATACTCAGG ATTCAAACCT GCCCAATATT CCCTTTCACG CTTCCAAGCA TTCTCAAATT 
CTACCTATGG ATGATTCAGA TGTAATAAAA TTGGCCAATG GAGATACCAG CATGAAACCA 
AATTCTGCTA CTAAAATTAG TCACTCAATG ACTTCTTTGC CTCTTCACCC ACTTCCACAA 
CCTTCTCAAA AGTCAAAGCA ATATCATATG ATATCCAAAT CAACCACTTC TTTGCCGCCA 
GAGAATGACC ACTACTATCA ACACAGTCGT GGCACCAACC ATAATCACGC TGCTAATGCT 
GCTGCTGTTA ATAATAATAC TACTACTACT ACCGCTGCTA CGGGTCTGAA AAGATCAGAG 
TCTGCAACGG CAGAAATTAA GAAGATGAGA CAATCTTTGC TGCATAAAAG AGAAATGAAA 
AGGAAAAGAA AAACATTTTT GGTGGATGAC GATCGTGTTT TGATCGGTAA TAAAGTCAGT 
GAAGGCCATG TCAACTTTAT TATAGCTTAT AACATGCTAA CTGGTATTCG TGTCGCAGTG 
TCACGTTGTT CTGGCATAAT GAAACCTTTA ACTCCGGCAG ACTTTAGATT TACGAAGAAG 
CTTGCCTTTG ATTATCACGG AAATGAATTG ACCCCTTCTT CTCAGTATGC ATTTAAGTTT 
AAGGACTACT GTCCCGAAGT CTTTAGGGAA CTTCGTGCAT TATTCGGCTT AGACCCTGCT 
GATTATTTGG TTTCGTTAAC TTCCAAGTAC ATTTTGAGTG AGTTGAACTC GCCAGGTAAA 
AGTGGTTCAT TTTTTTATTA TTCGAGAGAT TACAAATATA TTATCAAGAC CATACATCAT 
TCTGAGCATA TTCATCTGAG AAAGCACATA CAAGAATACT ATAACCATGT AAGAGACAAT 
CCAAACACTT TGATTTGTCA ATTTTATGGT TTGCATAGAG TGAAAATGCC TATATCGTTT 
CAAAATAAAA TTAAGCATCG GAAAATTTAC TTCCTAGTCA TGAATAACTT ATTTCCACCA 
CACTTAGACA TTCACATTAC TTATGATTTA AAAGGTTCCA CATGGGGCCG TTTTACCAAT 
TTGGATAAAG AAAGGTTGGC GAAAGATAGA TCATATAGGC CTGTGATGAA AGATTTAAAT 
TGGCTTGAAG AAGGTCAGAA AATTAAATTC GGTCCATTGA AGAAGAAGAC TTTTTTGACA 
CAGCTGAAAA AAGATGTGGA ATTGCTTGCT AAATTGAATA CAATGGACTA TTCCTTGTTA 
ATTGGCATTC ATGACATCAA TAAAGCTAAA GAAGACGACT TACAATTGGC GGATACGGCA 
TCTATTGAGG AGCAACCACA GACTCAAGGA CCCATAAGAA CCGGTACCGG AACGGTAGTA 
CGACATTTTT TTAGAGAGTT TGAAGGTGGA ATTCGAGCAT CTGATCAATT CAACAATGAT 
GTGGATTTGA TTTATTACGT GGGGATAATC GATTTCTTGA CTAATTACTC AGTTATGAAG 
AAATTAGAAA CGTTTTGGAG AAGTCTACGC CATGATACCA AGTTGGTGAG TGCCATACCT 
CCAAGAGACT ACGCCAATAG GTTTTACGAA TTCATAGAAG ATTCTGTAGA TCCTCTGCCC 
CAGAAAAAAA CTCAATCGTC GTATAGAGAC GACCCTAACC AGAAAAATTA TAAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MSS4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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