RVB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	RVB1
Entrez Gene ID	851771
Full Name	RuvB family ATP-dependent DNA helicase pontin
Synonyms	TIH1,TIP49,TIP49A
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180498.1	NP_010476.1	RuvB family ATP-dependent DNA helicase pontin

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RUVBL1	NP_001233458.1
	RUVBL1	XP_002798758.1
Gallus gallus (chicken)	RUVBL1	NP_001006138.1
Drosophila melanogaster (fruit fly)	pont	NP_652608.1
Caenorhabditis elegans (roundworm)	ruvb-1	NP_505567.2
Saccharomyces cerevisiae (baker's yeast)	RVB1	NP_010476.1
Eremothecium gossypii	AGOS_AGL119C	NP_986548.1
Homo sapiens (human)	RUVBL1	NP_003698.1
Mus musculus (house mouse)	Ruvbl1	NP_062659.1
Rattus norvegicus (Norway rat)	Ruvbl1	NP_671706.1
Magnaporthe oryzae (rice blast fungus)	MGG_03958	XP_003719906.1
Oryza sativa (rice)	Os01g0837500	NP_001044738.1
Xenopus tropicalis (tropical clawed frog)	ruvbl1	XP_002933113.1
Canis lupus familiaris (dog)	RUVBL1	XP_848712.1
Bos taurus (cattle)	RUVBL1	NP_001094546.1
Danio rerio (zebrafish)	ruvbl1	NP_776196.2
Kluyveromyces lactis	KLLA0D18502g	XP_453880.1
Schizosaccharomyces pombe (fission yeast)	rvb1	NP_594783.1
Neurospora crassa	NCU03482	XP_955769.1
Arabidopsis thaliana (thale cress)	RIN1	NP_197625.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the 14 different polypeptide containing chromatin-remodeling complex SWR1 contains a single heterohexameric Rvb1/Rvb2 ring that, together with the catalytic subunit Swr1, brackets two independently assembled multisubunit modules.
Title: Molecular architecture of the ATP-dependent chromatin-remodeling complex SWR1.

purified endogenous untagged Rvb1/Rvb2 complexes were found as single hexameric rings, similar to the complexes assembled in vitro from purified untagged components
Title: Alternative oligomeric states of the yeast Rvb1/Rvb2 complex induced by histidine tags.

Pontin and reptin could acquire distinct structural states to regulate their broad functions as molecular motors and scaffolds for nucleic acids and proteins.
Title: Architecture of the pontin/reptin complex, essential in the assembly of several macromolecular complexes.

These results clearly establish the yeast Rvb1/2 complex as a heterohexameric ATP-dependent DNA helicase and highlight the possible roles played by the Rvb proteins within multiprotein complexes.
Title: Yeast Rvb1 and Rvb2 are ATP-dependent DNA helicases that form a heterohexameric complex.

Rvb2p preferentially binds artificial DNA Holiday junctions like the bacterial RuvAB complex, whereas Rvb1p binds to duplex or cruciform DNA; both proteins also interact with chromatin and play a role in recombination and repair by chromatin remodelling.
Title: Functional and comparative characterization of Saccharomyces cerevisiae RVB1 and RVB2 genes with bacterial Ruv homologues.

The following RVB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RVB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01273	NM_001180498.1 Latest version!	Saccharomyces cerevisiae S288C RuvB family ATP-dependent DNA helicase pontin (RVB1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RVB1 ( NM_001180498.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010476.1 Saccharomyces cerevisiae S288C RuvB family ATP-dependent DNA helicase pontin (RVB1), partial mRNA.

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CloneID	OSi01273
Clone ID Related Accession (Same CDS sequence)	NM_001180498.1
Accession Version	NM_001180498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	RuvB family ATP-dependent DNA helicase pontin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTCGCTA TCAGTGAAGT CAAAGAAAAT CCTGGCGTAA ACAGCAGTAA TTCTGGTGCA 
GTTACGAGAA CTGCGGCACA TACACATATT AAAGGTTTAG GTCTGGATGA AAGTGGTGTG 
GCCAAGAGGG TTGAAGGAGG GTTTGTTGGA CAAATAGAAG CTCGTGAAGC GTGCGGTGTA 
ATTGTAGATT TAATCAAGGC TAAAAAAATG TCAGGTAGAG CTATCCTATT AGCTGGTGGT 
CCTTCTACAG GTAAGACAGC ATTGGCTCTC GCCATTTCAC AAGAGTTAGG CCCAAAAGTT 
CCATTCTGTC CTCTTGTTGG TAGCGAATTA TATTCCGTGG AGGTCAAAAA AACAGAAACC 
CTAATGGAAA ATTTTAGAAG AGCAATTGGG TTAAGAATCA AAGAAACTAA GGAAGTTTAT 
GAGGGTGAAG TGACAGAACT AACCCCTGAA GACGCAGAGA ATCCGTTGGG CGGATATGGT 
AAAACTATCT CACATGTAAT TGTCGGGCTC AAGTCTGCCA AGGGTACCAA GACACTAAGA 
TTAGACCCAA CGATATATGA AAGTATTCAA AGAGAAAAGG TTAGTATTGG TGATGTCATT 
TATATAGAGG CCAACACCGG TGCAGTTAAG CGAGTTGGCA GATCTGATGC ATACGCTACT 
GAATTTGATT TGGAAACTGA GGAATACGTA CCATTGCCAA AAGGCGAAGT GCATAAGAAA 
AAGGAAATTG TACAAGATGT TACCTTGCAC GATTTGGATG TTGCAAACGC AAGACCACAA 
GGTGGTCAAG ATGTCATATC AATGATGGGC CAGTTGCTCA AACCTAAGAA GACTGAAATA 
ACCGAGAAAT TAAGACAAGA GGTGAATAAA GTTGTTGCTA AATATATTGA TCAAGGTGTC 
GCAGAACTTA TTCCAGGTGT TTTGTTCATT GATGAAGTTA ATATGCTAGA CATTGAAATA 
TTCACGTATT TGAACAAAGC GCTTGAATCT AATATCGCCC CCGTTGTTGT ATTGGCTTCT 
AATAGGGGCA TGACTACAGT CCGTGGCACT GAGGATGTGA TATCACCACA TGGTGTGCCA 
CCTGATTTGA TCGATAGATT GTTAATTGTT CGTACATTAC CATATGACAA GGACGAGATC 
CGTACCATTA TAGAGAGAAG AGCTACCGTT GAAAGATTGC AAGTAGAAAG TAGCGCATTG 
GACCTTTTAG CCACGATGGG TACAGAAACT TCGTTACGTT ACGCTTTACA ATTATTGGCA 
CCATGTGGTA TCCTAGCACA AACAAGCAAT CGTAAGGAAA TTGTTGTTAA TGACGTTAAT 
GAAGCCAAAT TGCTGTTTTT GGATGCCAAA AGGTCAACAA AGATTTTAGA AACTTCCGCA 
AATTATTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010476.1
CDS	1..1392
Translation	MVAISEVKENPGVNSSNSGAVTRTAAHTHIKGLGLDESGVAKRVEGGFVGQIEAREACGVIVDLIKAKKMSGRAILLAGGPSTGKTALALAISQELGPKVPFCPLVGSELYSVEVKKTETLMENFRRAIGLRIKETKEVYEGEVTELTPEDAENPLGGYGKTISHVIVGLKSAKGTKTLRLDPTIYESIQREKVSIGDVIYIEANTGAVKRVGRSDAYATEFDLETEEYVPLPKGEVHKKKEIVQDVTLHDLDVANARPQGGQDVISMMGQLLKPKKTEITEKLRQEVNKVVAKYIDQGVAELIPGVLFIDEVNMLDIEIFTYLNKALESNIAPVVVLASNRGMTTVRGTEDVISPHGVPPDLIDRLLIVRTLPYDKDEIRTIIERRATVERLQVESSALDLLATMGTETSLRYALQLLAPCGILAQTSNRKEIVVNDVNEAKLLFLDAKRSTKILETSANYL

Target ORF information:

RefSeq Version	NM_001180498.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C RuvB family ATP-dependent DNA helicase pontin (RVB1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180498.1

ORF Insert Sequence:

ATGGTCGCTA TCAGTGAAGT CAAAGAAAAT CCTGGCGTAA ACAGCAGTAA TTCTGGTGCA 
GTTACGAGAA CTGCGGCACA TACACATATT AAAGGTTTAG GTCTGGATGA AAGTGGTGTG 
GCCAAGAGGG TTGAAGGAGG GTTTGTTGGA CAAATAGAAG CTCGTGAAGC GTGCGGTGTA 
ATTGTAGATT TAATCAAGGC TAAAAAAATG TCAGGTAGAG CTATCCTATT AGCTGGTGGT 
CCTTCTACAG GTAAGACAGC ATTGGCTCTC GCCATTTCAC AAGAGTTAGG CCCAAAAGTT 
CCATTCTGTC CTCTTGTTGG TAGCGAATTA TATTCCGTGG AGGTCAAAAA AACAGAAACC 
CTAATGGAAA ATTTTAGAAG AGCAATTGGG TTAAGAATCA AAGAAACTAA GGAAGTTTAT 
GAGGGTGAAG TGACAGAACT AACCCCTGAA GACGCAGAGA ATCCGTTGGG CGGATATGGT 
AAAACTATCT CACATGTAAT TGTCGGGCTC AAGTCTGCCA AGGGTACCAA GACACTAAGA 
TTAGACCCAA CGATATATGA AAGTATTCAA AGAGAAAAGG TTAGTATTGG TGATGTCATT 
TATATAGAGG CCAACACCGG TGCAGTTAAG CGAGTTGGCA GATCTGATGC ATACGCTACT 
GAATTTGATT TGGAAACTGA GGAATACGTA CCATTGCCAA AAGGCGAAGT GCATAAGAAA 
AAGGAAATTG TACAAGATGT TACCTTGCAC GATTTGGATG TTGCAAACGC AAGACCACAA 
GGTGGTCAAG ATGTCATATC AATGATGGGC CAGTTGCTCA AACCTAAGAA GACTGAAATA 
ACCGAGAAAT TAAGACAAGA GGTGAATAAA GTTGTTGCTA AATATATTGA TCAAGGTGTC 
GCAGAACTTA TTCCAGGTGT TTTGTTCATT GATGAAGTTA ATATGCTAGA CATTGAAATA 
TTCACGTATT TGAACAAAGC GCTTGAATCT AATATCGCCC CCGTTGTTGT ATTGGCTTCT 
AATAGGGGCA TGACTACAGT CCGTGGCACT GAGGATGTGA TATCACCACA TGGTGTGCCA 
CCTGATTTGA TCGATAGATT GTTAATTGTT CGTACATTAC CATATGACAA GGACGAGATC 
CGTACCATTA TAGAGAGAAG AGCTACCGTT GAAAGATTGC AAGTAGAAAG TAGCGCATTG 
GACCTTTTAG CCACGATGGG TACAGAAACT TCGTTACGTT ACGCTTTACA ATTATTGGCA 
CCATGTGGTA TCCTAGCACA AACAAGCAAT CGTAAGGAAA TTGTTGTTAA TGACGTTAAT 
GAAGCCAAAT TGCTGTTTTT GGATGCCAAA AGGTCAACAA AGATTTTAGA AACTTCCGCA 
AATTATTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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RVB1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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