YCF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YCF1
Entrez Gene ID	851713
Full Name	ATP-binding cassette glutathione S-conjugate transporter YCF1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180442.3	NP_010419.3	ATP-binding cassette glutathione S-conjugate transporter YCF1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ABCC3	XP_001158914.2
Canis lupus familiaris (dog)	ABCC3	XP_005624731.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009835	XP_553715.3
Schizosaccharomyces pombe (fission yeast)	abc2	NP_593943.3
Oryza sativa (rice)	Os04g0209300	NP_001052252.1
	ABCC3	XP_001094709.2
Drosophila melanogaster (fruit fly)	MRP	NP_995698.1
Caenorhabditis elegans (roundworm)	mrp-7	NP_507812.3
Eremothecium gossypii	AGOS_AGR047W	NP_986712.2
Magnaporthe oryzae (rice blast fungus)	MGG_01674	XP_003714662.1
Rattus norvegicus (Norway rat)	Abcc3	NP_542148.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008437	XP_317002.4
Caenorhabditis elegans (roundworm)	mrp-4	NP_509658.1
Caenorhabditis elegans (roundworm)	mrp-3	NP_510616.2
Schizosaccharomyces pombe (fission yeast)	abc3	NP_595055.1
Neurospora crassa	NCU09012	XP_958146.1
Xenopus tropicalis (tropical clawed frog)	abcc3	NP_001107301.1
Homo sapiens (human)	ABCC3	NP_003777.2
Mus musculus (house mouse)	Abcc3	NP_083876.3
Saccharomyces cerevisiae (baker's yeast)	YCF1	NP_010419.3
Arabidopsis thaliana (thale cress)	MRP10	NP_191829.1
Oryza sativa (rice)	Os04g0209200	NP_001052251.1
Bos taurus (cattle)	ABCC3	NP_001179685.1
Gallus gallus (chicken)	ABCC3	XP_420102.4
Kluyveromyces lactis	KLLA0F20075g	XP_455982.1
Arabidopsis thaliana (thale cress)	MRP4	NP_182301.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that deleting YCF1 caused a striking decrease in vacuolar levels of the soluble SNARE Vam7p, whereas total cellular levels were not altered.
Title: The yeast ATP-binding cassette (ABC) transporter Ycf1p enhances the recruitment of the soluble SNARE Vam7p to vacuoles for efficient membrane fusion.

Data show that Ycf1p plays an important role in cellular resistance to salt stress by maintaining the cellular redox balance via glutathione recycling.
Title: Ycf1p attenuates basal level oxidative stress response in Saccharomyces cerevisiae.

The authors provide strong evidence that Cka1p regulates Ycf1p function via phosphorylation of Ser251.
Title: Suppression of Ycf1p function by Cka1p-dependent phosphorylation is attenuated in response to salt stress.

Cka1p may regulate Ycf1p function through phosphorylation of Ser-251 either directly or indirectly.
Title: Negative regulation of the yeast ABC transporter Ycf1p by phosphorylation within its N-terminal extension.

Our data suggest that Ycf1p is involved in the maintenance of intracellular GSH homeostasis and it can interfere with the oxidative tolerance of yeast.
Title: The role of the yeast ATP-binding cassette Ycf1p in glutathione and cadmium ion homeostasis during respiratory metabolism.

The following YCF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YCF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01221	NM_001180442.3 Latest version!	Saccharomyces cerevisiae S288C ATP-binding cassette glutathione S-conjugate transporter YCF1 (YCF1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YCF1 ( NM_001180442.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010419.3 Saccharomyces cerevisiae S288C ATP-binding cassette glutathione S-conjugate transporter YCF1 (YCF1), partial mRNA.

Price & Availability↑

CloneID	OSi01221
Clone ID Related Accession (Same CDS sequence)	NM_001180442.3
Accession Version	NM_001180442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ATP-binding cassette glutathione S-conjugate transporter YCF1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180442.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCTGGTA ATCTTGTTTC ATGGGCCTGC AAGCTCTGTA GATCTCCTGA AGGGTTTGGA 
CCTATATCCT TTTACGGTGA CTTTACTCAA TGCTTCATCG ACGGTGTGAT CCTAAATCTA 
TCAGCAATTT TCATGATAAC CTTCGGTATC AGAGATTTAG TTAACCTTTG CAAGAAAAAA 
CACTCTGGCA TCAAATATAG GCGGAATTGG ATTATTGTCT CTAGGATGGC ACTAGTTCTG 
TTGGAGATAG CGTTTGTTTC ACTTGCGTCT TTAAATATTT CTAAAGAAGA AGCGGAAAAC 
TTTACCATTG TAAGTCAATA TGCTTCTACA ATGTTATCTT TATTTGTTGC TTTAGCCTTA 
CACTGGATAG AATACGATAG ATCAGTTGTA GCCAATACGG TACTTTTATT CTATTGGCTT 
TTTGAAACAT TCGGTAATTT TGCTAAACTA ATAAATATTC TAATTAGACA CACCTACGAA 
GGCATTTGGT ATTCCGGACA AACGGGTTTC ATACTAACGT TATTCCAAGT AATAACATGT 
GCCAGTATCC TGTTACTTGA AGCTCTTCCA AAGAAGCCGC TAATGCCACA TCAACACATA 
CATCAAACTT TAACAAGAAG AAAACCAAAT CCATACGATA GCGCAAACAT ATTTTCCAGG 
ATTACCTTCT CTTGGATGTC AGGTTTGATG AAAACTGGCT ATGAAAAATA CTTAGTGGAA 
GCAGATTTAT ATAAATTACC GAGGAACTTT AGTAGTGAAG AACTCTCTCA AAAATTGGAG 
AAAAACTGGG AAAATGAGTT GAAGCAAAAA TCAAATCCTT CATTATCATG GGCTATATGC 
AGAACTTTTG GATCTAAAAT GCTTTTAGCC GCATTCTTTA AAGCAATTCA TGATGTTCTA 
GCATTTACTC AACCACAACT ACTAAGGATT TTAATCAAGT TCGTCACAGA CTATAACAGT 
GAGAGACAGG ATGACCATTC TTCTCTTCAA GGGTTTGAAA ATAACCACCC ACAAAAATTA 
CCCATTGTAA GAGGGTTTTT GATTGCGTTT GCTATGTTTC TGGTGGGCTT TACTCAGACA 
TCTGTCCTGC ATCAATATTT CCTGAATGTC TTCAACACAG GCATGTATAT TAAGAGCGCC 
CTAACGGCTT TAATATATCA AAAATCCTTA GTGCTATCTA ATGAGGCTTC TGGACTTTCC 
TCTACCGGTG ACATTGTCAA TCTCATGAGT GTGGATGTTC AAAAATTACA AGATTTAACA 
CAATGGCTAA ATTTAATATG GTCAGGGCCT TTTCAAATCA TTATTTGCTT ATATTCTCTG 
TATAAGTTGT TGGGAAATTC CATGTGGGTT GGCGTGATTA TACTAGTTAT TATGATGCCA 
TTGAACTCAT TTTTGATGAG GATACAAAAG AAGTTGCAAA AATCCCAGAT GAAGTACAAA 
GATGAAAGGA CCCGTGTTAT AAGTGAAATA CTAAACAATA TTAAATCTTT GAAGTTATAT 
GCATGGGAGA AGCCTTATAG GGAAAAGCTA GAAGAAGTAA GAAATAACAA AGAGTTAAAA 
AATCTTACAA AACTAGGATG TTATATGGCT GTGACAAGTT TTCAGTTCAA TATAGTACCA 
TTCCTTGTTT CATGTTGTAC CTTTGCTGTA TTTGTTTATA CTGAGGATAG AGCATTGACT 
ACTGACTTAG TTTTCCCTGC TTTGACTCTG TTCAACCTGC TCTCATTCCC ACTAATGATT 
ATTCCTATGG TTTTAAATTC TTTTATCGAA GCTTCTGTTT CTATTGGTAG ATTATTTACA 
TTCTTTACCA ATGAAGAGCT ACAACCAGAT TCGGTTCAGC GTTTACCAAA AGTAAAAAAT 
ATTGGCGATG TAGCCATTAA CATTGGAGAT GATGCTACCT TTTTATGGCA ACGGAAACCG 
GAATACAAAG TAGCCTTAAA GAATATTAAT TTCCAAGCTA AAAAAGGAAA TTTGACCTGT 
ATTGTTGGTA AAGTTGGCAG TGGTAAAACA GCTCTATTGT CATGCATGTT AGGTGATCTA 
TTCAGGGTTA AAGGTTTCGC CACCGTTCAT GGTTCTGTTG CTTATGTTTC ACAAGTTCCA 
TGGATAATGA ATGGTACTGT AAAGGAAAAC ATTTTATTTG GGCATAGATA CGACGCGGAA 
TTTTACGAAA AAACGATCAA GGCCTGTGCG TTAACTATTG ATCTTGCAAT TTTGATGGAT 
GGAGATAAGA CATTAGTTGG CGAGAAAGGG ATCTCCTTAT CTGGAGGACA AAAAGCTCGT 
TTGTCTTTAG CAAGAGCAGT TTATGCGAGA GCTGACACTT ATTTACTTGA TGATCCTTTG 
GCAGCTGTTG ATGAACACGT TGCCAGGCAC TTGATCGAAC ATGTGTTGGG TCCAAATGGT 
TTATTACATA CAAAAACGAA GGTATTAGCC ACTAATAAGG TGAGCGCGTT ATCCATCGCA 
GATTCTATTG CATTATTAGA TAATGGAGAA ATCACACAGC AGGGTACATA TGATGAGATT 
ACGAAGGACG CTGATTCGCC ATTATGGAAA TTGCTCAACA ACTATGGTAA AAAAAATAAC 
GGTAAGTCGA ATGAATTCGG TGACTCCTCT GAAAGCTCAG TTCGAGAAAG TAGTATACCT 
GTAGAAGGAG AGCTGGAACA ACTGCAGAAA TTAAATGATT TGGATTTTGG CAACTCTGAC 
GCCATAAGTT TAAGGAGGGC CAGTGATGCA ACTTTGGGAA GCATCGATTT TGGTGACGAT 
GAAAATATTG CTAAAAGAGA GCATCGTGAA CAGGGAAAAG TAAAGTGGAA CATTTACCTA 
GAGTACGCTA AAGCTTGCAA CCCGAAAAGC GTTTGTGTAT TCATATTGTT TATTGTTATA 
TCGATGTTCC TCTCTGTTAT GGGTAACGTT TGGTTGAAAC ATTGGTCTGA AGTTAATAGC 
CGCTATGGAT CTAATCCAAA TGCCGCGCGT TACTTGGCCA TTTATTTTGC ACTTGGTATT 
GGTTCAGCAC TGGCAACATT AATCCAGACA ATCGTTCTCT GGGTTTTTTG TACCATTCAT 
GCCTCCAAAT ATTTACACAA CTTGATGACA AACTCTGTGT TGAGAGCCCC AATGACGTTT 
TTTGAAACAA CACCAATCGG TAGAATTCTA AACAGATTCT CAAATGACAT ATACAAAGTG 
GATGCTTTAT TAGGAAGAAC ATTTTCTCAG TTTTTCGTCA ATGCAGTGAA AGTCACATTC 
ACTATTACGG TTATCTGTGC GACGACATGG CAATTTATCT TCATTATCAT TCCACTAAGT 
GTGTTTTACA TCTACTACCA GCAGTATTAC CTGAGAACAT CAAGGGAGTT GCGTCGTTTA 
GACTCTATTA CTAGGTCTCC AATCTACTCT CATTTCCAAG AGACTTTGGG TGGCCTTGCA 
ACGGTTAGAG GTTATTCTCA ACAGAAAAGG TTTTCCCACA TTAATCAATG CCGCATTGAT 
AATAACATGA GTGCGTTCTA TCCCTCTATC AATGCTAACC GTTGGCTAGC ATATAGGTTG 
GAACTTATTG GTTCAATTAT CATTCTAGGT GCTGCAACTT TATCCGTTTT TAGACTAAAA 
CAAGGCACAT TAACGGCAGG TATGGTGGGT TTATCATTAA GCTATGCTTT ACAAATCACT 
CAAACGTTAA ATTGGATTGT TAGAATGACT GTGGAAGTTG AAACGAATAT TGTTTCAGTG 
GAAAGAATAA AGGAATATGC TGATTTGAAG AGCGAGGCAC CTTTAATAGT TGAAGGCCAC 
AGACCACCCA AAGAATGGCC GAGCCAGGGT GATATAAAGT TTAATAATTA TTCCACTCGT 
TATAGGCCGG AGCTTGATCT TGTTCTGAAG CACATTAATA TACACATTAA ACCAAATGAA 
AAAGTTGGTA TCGTGGGTAG AACGGGTGCG GGAAAATCCT CATTAACGCT AGCATTATTC 
AGGATGATTG AGGCTAGCGA GGGAAACATC GTAATCGACA ACATTGCCAT CAACGAGATT 
GGGTTATATG ATTTGAGACA TAAATTGTCA ATCATACCTC AGGATTCTCA AGTTTTTGAG 
GGCACTGTTC GTGAGAACAT TGATCCCATT AACCAATACA CTGATGAAGC TATTTGGAGG 
GCATTGGAAC TTTCTCATTT GAAAGAACAC GTGCTATCAA TGAGCAATGA CGGATTAGAT 
GCCCAACTAA CCGAAGGTGG TGGCAACTTA AGTGTTGGAC AAAGACAATT ATTATGTCTT 
GCAAGAGCAA TGTTGGTTCC ATCAAAGATT TTGGTGCTTG ATGAAGCCAC GGCCGCAGTC 
GACGTGGAGA CAGATAAAGT CGTCCAAGAG ACGATTCGTA CTGCTTTCAA GGACAGAACT 
ATCTTGACCA TCGCGCATAG ACTGAACACG ATAATGGACA GTGATAGAAT CATAGTGTTG 
GACAATGGTA AAGTAGCCGA GTTTGACTCT CCGGGCCAGT TATTAAGTGA TAACAAATCA 
TTGTTCTATT CACTGTGCAT GGAGGCTGGT TTGGTCAATG AAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010419.3
CDS	1..4548
Translation	MAGNLVSWACKLCRSPEGFGPISFYGDFTQCFIDGVILNLSAIFMITFGIRDLVNLCKKKHSGIKYRRNWIIVSRMALVLLEIAFVSLASLNISKEEAENFTIVSQYASTMLSLFVALALHWIEYDRSVVANTVLLFYWLFETFGNFAKLINILIRHTYEGIWYSGQTGFILTLFQVITCASILLLEALPKKPLMPHQHIHQTLTRRKPNPYDSANIFSRITFSWMSGLMKTGYEKYLVEADLYKLPRNFSSEELSQKLEKNWENELKQKSNPSLSWAICRTFGSKMLLAAFFKAIHDVLAFTQPQLLRILIKFVTDYNSERQDDHSSLQGFENNHPQKLPIVRGFLIAFAMFLVGFTQTSVLHQYFLNVFNTGMYIKSALTALIYQKSLVLSNEASGLSSTGDIVNLMSVDVQKLQDLTQWLNLIWSGPFQIIICLYSLYKLLGNSMWVGVIILVIMMPLNSFLMRIQKKLQKSQMKYKDERTRVISEILNNIKSLKLYAWEKPYREKLEEVRNNKELKNLTKLGCYMAVTSFQFNIVPFLVSCCTFAVFVYTEDRALTTDLVFPALTLFNLLSFPLMIIPMVLNSFIEASVSIGRLFTFFTNEELQPDSVQRLPKVKNIGDVAINIGDDATFLWQRKPEYKVALKNINFQAKKGNLTCIVGKVGSGKTALLSCMLGDLFRVKGFATVHGSVAYVSQVPWIMNGTVKENILFGHRYDAEFYEKTIKACALTIDLAILMDGDKTLVGEKGISLSGGQKARLSLARAVYARADTYLLDDPLAAVDEHVARHLIEHVLGPNGLLHTKTKVLATNKVSALSIADSIALLDNGEITQQGTYDEITKDADSPLWKLLNNYGKKNNGKSNEFGDSSESSVRESSIPVEGELEQLQKLNDLDFGNSDAISLRRASDATLGSIDFGDDENIAKREHREQGKVKWNIYLEYAKACNPKSVCVFILFIVISMFLSVMGNVWLKHWSEVNSRYGSNPNAARYLAIYFALGIGSALATLIQTIVLWVFCTIHASKYLHNLMTNSVLRAPMTFFETTPIGRILNRFSNDIYKVDALLGRTFSQFFVNAVKVTFTITVICATTWQFIFIIIPLSVFYIYYQQYYLRTSRELRRLDSITRSPIYSHFQETLGGLATVRGYSQQKRFSHINQCRIDNNMSAFYPSINANRWLAYRLELIGSIIILGAATLSVFRLKQGTLTAGMVGLSLSYALQITQTLNWIVRMTVEVETNIVSVERIKEYADLKSEAPLIVEGHRPPKEWPSQGDIKFNNYSTRYRPELDLVLKHINIHIKPNEKVGIVGRTGAGKSSLTLALFRMIEASEGNIVIDNIAINEIGLYDLRHKLSIIPQDSQVFEGTVRENIDPINQYTDEAIWRALELSHLKEHVLSMSNDGLDAQLTEGGGNLSVGQRQLLCLARAMLVPSKILVLDEATAAVDVETDKVVQETIRTAFKDRTILTIAHRLNTIMDSDRIIVLDNGKVAEFDSPGQLLSDNKSLFYSLCMEAGLVNEN

Target ORF information:

RefSeq Version	NM_001180442.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ATP-binding cassette glutathione S-conjugate transporter YCF1 (YCF1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180442.3

ORF Insert Sequence:

ATGGCTGGTA ATCTTGTTTC ATGGGCCTGC AAGCTCTGTA GATCTCCTGA AGGGTTTGGA 
CCTATATCCT TTTACGGTGA CTTTACTCAA TGCTTCATCG ACGGTGTGAT CCTAAATCTA 
TCAGCAATTT TCATGATAAC CTTCGGTATC AGAGATTTAG TTAACCTTTG CAAGAAAAAA 
CACTCTGGCA TCAAATATAG GCGGAATTGG ATTATTGTCT CTAGGATGGC ACTAGTTCTG 
TTGGAGATAG CGTTTGTTTC ACTTGCGTCT TTAAATATTT CTAAAGAAGA AGCGGAAAAC 
TTTACCATTG TAAGTCAATA TGCTTCTACA ATGTTATCTT TATTTGTTGC TTTAGCCTTA 
CACTGGATAG AATACGATAG ATCAGTTGTA GCCAATACGG TACTTTTATT CTATTGGCTT 
TTTGAAACAT TCGGTAATTT TGCTAAACTA ATAAATATTC TAATTAGACA CACCTACGAA 
GGCATTTGGT ATTCCGGACA AACGGGTTTC ATACTAACGT TATTCCAAGT AATAACATGT 
GCCAGTATCC TGTTACTTGA AGCTCTTCCA AAGAAGCCGC TAATGCCACA TCAACACATA 
CATCAAACTT TAACAAGAAG AAAACCAAAT CCATACGATA GCGCAAACAT ATTTTCCAGG 
ATTACCTTCT CTTGGATGTC AGGTTTGATG AAAACTGGCT ATGAAAAATA CTTAGTGGAA 
GCAGATTTAT ATAAATTACC GAGGAACTTT AGTAGTGAAG AACTCTCTCA AAAATTGGAG 
AAAAACTGGG AAAATGAGTT GAAGCAAAAA TCAAATCCTT CATTATCATG GGCTATATGC 
AGAACTTTTG GATCTAAAAT GCTTTTAGCC GCATTCTTTA AAGCAATTCA TGATGTTCTA 
GCATTTACTC AACCACAACT ACTAAGGATT TTAATCAAGT TCGTCACAGA CTATAACAGT 
GAGAGACAGG ATGACCATTC TTCTCTTCAA GGGTTTGAAA ATAACCACCC ACAAAAATTA 
CCCATTGTAA GAGGGTTTTT GATTGCGTTT GCTATGTTTC TGGTGGGCTT TACTCAGACA 
TCTGTCCTGC ATCAATATTT CCTGAATGTC TTCAACACAG GCATGTATAT TAAGAGCGCC 
CTAACGGCTT TAATATATCA AAAATCCTTA GTGCTATCTA ATGAGGCTTC TGGACTTTCC 
TCTACCGGTG ACATTGTCAA TCTCATGAGT GTGGATGTTC AAAAATTACA AGATTTAACA 
CAATGGCTAA ATTTAATATG GTCAGGGCCT TTTCAAATCA TTATTTGCTT ATATTCTCTG 
TATAAGTTGT TGGGAAATTC CATGTGGGTT GGCGTGATTA TACTAGTTAT TATGATGCCA 
TTGAACTCAT TTTTGATGAG GATACAAAAG AAGTTGCAAA AATCCCAGAT GAAGTACAAA 
GATGAAAGGA CCCGTGTTAT AAGTGAAATA CTAAACAATA TTAAATCTTT GAAGTTATAT 
GCATGGGAGA AGCCTTATAG GGAAAAGCTA GAAGAAGTAA GAAATAACAA AGAGTTAAAA 
AATCTTACAA AACTAGGATG TTATATGGCT GTGACAAGTT TTCAGTTCAA TATAGTACCA 
TTCCTTGTTT CATGTTGTAC CTTTGCTGTA TTTGTTTATA CTGAGGATAG AGCATTGACT 
ACTGACTTAG TTTTCCCTGC TTTGACTCTG TTCAACCTGC TCTCATTCCC ACTAATGATT 
ATTCCTATGG TTTTAAATTC TTTTATCGAA GCTTCTGTTT CTATTGGTAG ATTATTTACA 
TTCTTTACCA ATGAAGAGCT ACAACCAGAT TCGGTTCAGC GTTTACCAAA AGTAAAAAAT 
ATTGGCGATG TAGCCATTAA CATTGGAGAT GATGCTACCT TTTTATGGCA ACGGAAACCG 
GAATACAAAG TAGCCTTAAA GAATATTAAT TTCCAAGCTA AAAAAGGAAA TTTGACCTGT 
ATTGTTGGTA AAGTTGGCAG TGGTAAAACA GCTCTATTGT CATGCATGTT AGGTGATCTA 
TTCAGGGTTA AAGGTTTCGC CACCGTTCAT GGTTCTGTTG CTTATGTTTC ACAAGTTCCA 
TGGATAATGA ATGGTACTGT AAAGGAAAAC ATTTTATTTG GGCATAGATA CGACGCGGAA 
TTTTACGAAA AAACGATCAA GGCCTGTGCG TTAACTATTG ATCTTGCAAT TTTGATGGAT 
GGAGATAAGA CATTAGTTGG CGAGAAAGGG ATCTCCTTAT CTGGAGGACA AAAAGCTCGT 
TTGTCTTTAG CAAGAGCAGT TTATGCGAGA GCTGACACTT ATTTACTTGA TGATCCTTTG 
GCAGCTGTTG ATGAACACGT TGCCAGGCAC TTGATCGAAC ATGTGTTGGG TCCAAATGGT 
TTATTACATA CAAAAACGAA GGTATTAGCC ACTAATAAGG TGAGCGCGTT ATCCATCGCA 
GATTCTATTG CATTATTAGA TAATGGAGAA ATCACACAGC AGGGTACATA TGATGAGATT 
ACGAAGGACG CTGATTCGCC ATTATGGAAA TTGCTCAACA ACTATGGTAA AAAAAATAAC 
GGTAAGTCGA ATGAATTCGG TGACTCCTCT GAAAGCTCAG TTCGAGAAAG TAGTATACCT 
GTAGAAGGAG AGCTGGAACA ACTGCAGAAA TTAAATGATT TGGATTTTGG CAACTCTGAC 
GCCATAAGTT TAAGGAGGGC CAGTGATGCA ACTTTGGGAA GCATCGATTT TGGTGACGAT 
GAAAATATTG CTAAAAGAGA GCATCGTGAA CAGGGAAAAG TAAAGTGGAA CATTTACCTA 
GAGTACGCTA AAGCTTGCAA CCCGAAAAGC GTTTGTGTAT TCATATTGTT TATTGTTATA 
TCGATGTTCC TCTCTGTTAT GGGTAACGTT TGGTTGAAAC ATTGGTCTGA AGTTAATAGC 
CGCTATGGAT CTAATCCAAA TGCCGCGCGT TACTTGGCCA TTTATTTTGC ACTTGGTATT 
GGTTCAGCAC TGGCAACATT AATCCAGACA ATCGTTCTCT GGGTTTTTTG TACCATTCAT 
GCCTCCAAAT ATTTACACAA CTTGATGACA AACTCTGTGT TGAGAGCCCC AATGACGTTT 
TTTGAAACAA CACCAATCGG TAGAATTCTA AACAGATTCT CAAATGACAT ATACAAAGTG 
GATGCTTTAT TAGGAAGAAC ATTTTCTCAG TTTTTCGTCA ATGCAGTGAA AGTCACATTC 
ACTATTACGG TTATCTGTGC GACGACATGG CAATTTATCT TCATTATCAT TCCACTAAGT 
GTGTTTTACA TCTACTACCA GCAGTATTAC CTGAGAACAT CAAGGGAGTT GCGTCGTTTA 
GACTCTATTA CTAGGTCTCC AATCTACTCT CATTTCCAAG AGACTTTGGG TGGCCTTGCA 
ACGGTTAGAG GTTATTCTCA ACAGAAAAGG TTTTCCCACA TTAATCAATG CCGCATTGAT 
AATAACATGA GTGCGTTCTA TCCCTCTATC AATGCTAACC GTTGGCTAGC ATATAGGTTG 
GAACTTATTG GTTCAATTAT CATTCTAGGT GCTGCAACTT TATCCGTTTT TAGACTAAAA 
CAAGGCACAT TAACGGCAGG TATGGTGGGT TTATCATTAA GCTATGCTTT ACAAATCACT 
CAAACGTTAA ATTGGATTGT TAGAATGACT GTGGAAGTTG AAACGAATAT TGTTTCAGTG 
GAAAGAATAA AGGAATATGC TGATTTGAAG AGCGAGGCAC CTTTAATAGT TGAAGGCCAC 
AGACCACCCA AAGAATGGCC GAGCCAGGGT GATATAAAGT TTAATAATTA TTCCACTCGT 
TATAGGCCGG AGCTTGATCT TGTTCTGAAG CACATTAATA TACACATTAA ACCAAATGAA 
AAAGTTGGTA TCGTGGGTAG AACGGGTGCG GGAAAATCCT CATTAACGCT AGCATTATTC 
AGGATGATTG AGGCTAGCGA GGGAAACATC GTAATCGACA ACATTGCCAT CAACGAGATT 
GGGTTATATG ATTTGAGACA TAAATTGTCA ATCATACCTC AGGATTCTCA AGTTTTTGAG 
GGCACTGTTC GTGAGAACAT TGATCCCATT AACCAATACA CTGATGAAGC TATTTGGAGG 
GCATTGGAAC TTTCTCATTT GAAAGAACAC GTGCTATCAA TGAGCAATGA CGGATTAGAT 
GCCCAACTAA CCGAAGGTGG TGGCAACTTA AGTGTTGGAC AAAGACAATT ATTATGTCTT 
GCAAGAGCAA TGTTGGTTCC ATCAAAGATT TTGGTGCTTG ATGAAGCCAC GGCCGCAGTC 
GACGTGGAGA CAGATAAAGT CGTCCAAGAG ACGATTCGTA CTGCTTTCAA GGACAGAACT 
ATCTTGACCA TCGCGCATAG ACTGAACACG ATAATGGACA GTGATAGAAT CATAGTGTTG 
GACAATGGTA AAGTAGCCGA GTTTGACTCT CCGGGCCAGT TATTAAGTGA TAACAAATCA 
TTGTTCTATT CACTGTGCAT GGAGGCTGGT TTGGTCAATG AAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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YCF1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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