STE5 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STE5
Entrez Gene ID	851680
Full Name	Ste5p
Synonyms	HMD3,NUL3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180411.1	NP_010388.1	Ste5p

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	STE5	NP_010388.1
Kluyveromyces lactis	KLLA0F12023g	XP_455625.1
Eremothecium gossypii	AGOS_AGR104W	NP_986770.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

defining regions of Ste5 that mediate nuclear export
Title: Mapping regions in Ste5 that support Msn5-dependent and -independent nuclear export.

The Ras-binding-domain-like region in the Ste11 protein that is required specifically for the kinase to function with Ste5 in the mating pathway
Title: Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling.

findings show that the scaffold protein Ste5, which organizes the yeast mating mitogen-activated protein kinase pathway, does not use sequestration to prevent misactivation of the mating response; Ste5 appears to use a conformation mechanism
Title: Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation.

Structural determinants of the specificity of a membrane binding domain of the scaffold protein Ste5 of budding yeast: implications in signaling by the scaffold protein in MAPK pathway.
Title: Structural determinants of the specificity of a membrane binding domain of the scaffold protein Ste5 of budding yeast: implications in signaling by the scaffold protein in MAPK pathway.

The signaling proteins Ste5 and Ste20 have recognition sequences, or "docking" sites, for G1/S (interphase) cyclins. These docking sites bind preferentially to the cyclin Cln2, and strongly enhance Cln2-driven in vivo substrate phosphorylation.
Title: Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes.

The following STE5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STE5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01192	NM_001180411.1 Latest version!	Saccharomyces cerevisiae S288C Ste5p (STE5), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STE5 ( NM_001180411.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010388.1 Saccharomyces cerevisiae S288C Ste5p (STE5), partial mRNA.

Price & Availability↑

CloneID	OSi01192
Clone ID Related Accession (Same CDS sequence)	NM_001180411.1
Accession Version	NM_001180411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Ste5p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGATGGAAA CTCCTACAGA CAATATAGTT TCCCCTTTTC ACAATTTTGG TAGCTCGACA 
CAATATAGTG GTACCTTGTC GAGAACTCCC AACCAAATAA TAGAGCTAGA GAAGCCCAGT 
ACTCTATCCC CATTGTCAAG AGGAAAAAAA TGGACGGAAA AGTTAGCCAG GTTCCAAAGA 
AGTAGTGCTA AAAAGAAAAG ATTCTCACCT TCTCCTATTT CCTCCTCTAC ATTTTCGTTC 
TCACCCAAAT CTAGGGTCAC TTCTTCAAAC TCTTCTGGCA ATGAAGACGG TAACCTAATG 
AATACACCTT CTACGGTTTC CACTGATTAT TTGCCACAAC ACCCTCACAG AACATCGTCT 
TTGCCAAGAC CTAATTCCAA TCTCTTTCAC GCAAGTAATA GTAACCTATC CCGAGCAAAT 
GAGCCCCCAA GGGCCGAAAA TTTATCAGAT AATATACCAC CCAAGGTCGC TCCATTTGGC 
TATCCAATAC AAAGAACCTC TATTAAAAAA TCCTTTTTGA ATGCTTCTTG TACGTTATGT 
GACGAGCCTA TTTCTAACAG AAGAAAGGGA GAGAAAATTA TAGAGCTTGC ATGTGGCCAC 
TTAAGTCACC AAGAATGTCT TATTATCTCT TTTGGCACCA CTTCAAAGGC AGACGTTCGT 
GCGCTATTTC CTTTTTGTAC CAAATGTAAA AAAGATACTA ACAAAGCCGT TCAATGCATT 
CCAGAAAATG ATGAACTAAA GGATATTCTA ATTTCTGATT TTTTGATTCA TAAGATTCCT 
GATTCTGAGT TATCAATCAC ACCTCAGTCC CGCTTTCCTC CTTATTCACC ACTCTTGCCT 
CCTTTTGGGT TATCCTATAC ACCTGTTGAA AGACAAACGA TATATTCTCA AGCTCCAAGT 
CTAAACCCAA ATCTCATATT GGCTGCACCC CCCAAGGAAA GAAACCAAAT TCCACAAAAA 
AAATCAAACT ATACATTTTT ACATTCACCC CTGGGGCACA GAAGAATTCC GTCCGGAGCA 
AACTCTATCT TAGCAGACAC CTCTGTAGCG TTGTCAGCTA ATGATTCTAT TTCTGCTGTT 
TCCAATTCGG TAAGAGCAAA GGATGACGAA ACCAAAACAA CGTTGCCGCT GTTAAGGTCA 
TATTTTATTC AAATTCTTTT GAACAATTTC CAGGAAGAAT TGCAGGATTG GAGAATAGAC 
GGGGACTATG GATTACTAAG GTTGGTAGAC AAATTGATGA TTTCCAAAGA TGGTCAGAGA 
TATATACAAT GCTGGTGTTT CTTATTTGAA GACGCATTTG TAATAGCAGA AGTGGATAAC 
GATGTTGATG TTTTGGAAAT TAGACTAAAG AATTTAGAAG TATTTACACC TATTGCCAAC 
TTGAGAATGA CTACACTCGA AGCTTCAGTA CTCAAATGCA CCTTAAATAA ACAACATTGC 
GCCGATTTAT CAGATCTTTA CATTGTTCAG AATATAAATT CTGACGAAAG CACAACTGTA 
CAGAAATGGA TATCAGGTAT ATTGAATCAG GATTTTGTAT TCAATGAGGA CAATATCACT 
TCGACCCTGC CTATTCTTCC CATTATAAAG AACTTTTCAA AAGATGTTGG TAATGGTAGG 
CACGAGACGA GTACCTTTCT AGGTTTAATC AATCCTAACA AAGTTGTTGA AGTTGGAAAT 
GTGCACGATA ATGATACTGT AATCATAAGG AGGGGATTCA CCTTAAATTC AGGAGAATGT 
TCTAGGCAGA GTACTGTCGA CAGTATACAA TCTGTTCTAA CCACGATAAG CTCAATTCTT 
TCCCTTAAAC GAGAAAAACC TGATAATTTG GCAATAATCT TACAGATCGA TTTTACGAAA 
TTGAAGGAAG AAGACAGTTT AATTGTTGTT TATAACAGTC TAAAAGCTTT AACCATTAAA 
TTTGCGCGTT TGCAGTTTTG TTTCGTTGAT CGAAATAATT ATGTTCTGGA CTATGGATCG 
GTATTACACA AGATAGATTC ACTAGATTCC ATCTCAAATC TCAAATCAAA GAGTTCCTCG 
ACACAATTTT CACCTATTTG GTTGAAAAAT ACTCTATATC CCGAAAATAT TCATGAACAT 
TTGGGTATTG TTGCTGTATC AAATAGTAAT ATGGAAGCAA AAAAATCCAT ACTATTTCAA 
GATTACAGAT GCTTTACAAG TTTTGGAAGA AGAAGGCCCA ATGAATTGAA GATTAAGGTG 
GGCTATTTGA ACGTTGACTA CAGTGATAAA ATTGATGAAC TAGTCGAGGC CAGCTCCTGG 
ACTTTTGTTT TAGAAACTCT TTGCTACAGT TTCGGTCTAA GTTTTGATGA ACATGATGAC 
GATGACGAAG AGGATAATGA TGATTCGACC GATAATGAAC TTGATAATAG TTCAGGATCA 
CTGTCGGATG CTGAATCTAC AACTACTATT CATATTGATT CTCCATTTGA TAATGAAAAT 
GCTACCGCAA ATATGGTGAA TGACAGAAAC CTTCTCACTG AGGGTGAACA TAGCAATATA 
GAAAACTTAG AAACTGTCGC TTCTTCAGTA CAGCCAGCTC TGATTCCTAA TATTAGATTT 
TCACTTCATT CTGAGGAGGA AGGTACTAAT GAAAATGAAA ATGAAAATGA TATGCCAGTA 
TTATTACTTA GTGATATGGA TAAAGGAATC GATGGCATAA CCAGACGCAG TTCATTCTCG 
AGTCTTATAG AGAGCGGTAA TAACAACTGT CCCCTCCATA TGGATTATAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010388.1
CDS	1..2754
Translation	MMETPTDNIVSPFHNFGSSTQYSGTLSRTPNQIIELEKPSTLSPLSRGKKWTEKLARFQRSSAKKKRFSPSPISSSTFSFSPKSRVTSSNSSGNEDGNLMNTPSTVSTDYLPQHPHRTSSLPRPNSNLFHASNSNLSRANEPPRAENLSDNIPPKVAPFGYPIQRTSIKKSFLNASCTLCDEPISNRRKGEKIIELACGHLSHQECLIISFGTTSKADVRALFPFCTKCKKDTNKAVQCIPENDELKDILISDFLIHKIPDSELSITPQSRFPPYSPLLPPFGLSYTPVERQTIYSQAPSLNPNLILAAPPKERNQIPQKKSNYTFLHSPLGHRRIPSGANSILADTSVALSANDSISAVSNSVRAKDDETKTTLPLLRSYFIQILLNNFQEELQDWRIDGDYGLLRLVDKLMISKDGQRYIQCWCFLFEDAFVIAEVDNDVDVLEIRLKNLEVFTPIANLRMTTLEASVLKCTLNKQHCADLSDLYIVQNINSDESTTVQKWISGILNQDFVFNEDNITSTLPILPIIKNFSKDVGNGRHETSTFLGLINPNKVVEVGNVHDNDTVIIRRGFTLNSGECSRQSTVDSIQSVLTTISSILSLKREKPDNLAIILQIDFTKLKEEDSLIVVYNSLKALTIKFARLQFCFVDRNNYVLDYGSVLHKIDSLDSISNLKSKSSSTQFSPIWLKNTLYPENIHEHLGIVAVSNSNMEAKKSILFQDYRCFTSFGRRRPNELKIKVGYLNVDYSDKIDELVEASSWTFVLETLCYSFGLSFDEHDDDDEEDNDDSTDNELDNSSGSLSDAESTTTIHIDSPFDNENATANMVNDRNLLTEGEHSNIENLETVASSVQPALIPNIRFSLHSEEEGTNENENENDMPVLLLSDMDKGIDGITRRSSFSSLIESGNNNCPLHMDYI

Target ORF information:

RefSeq Version	NM_001180411.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Ste5p (STE5), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180411.1

ORF Insert Sequence:

ATGATGGAAA CTCCTACAGA CAATATAGTT TCCCCTTTTC ACAATTTTGG TAGCTCGACA 
CAATATAGTG GTACCTTGTC GAGAACTCCC AACCAAATAA TAGAGCTAGA GAAGCCCAGT 
ACTCTATCCC CATTGTCAAG AGGAAAAAAA TGGACGGAAA AGTTAGCCAG GTTCCAAAGA 
AGTAGTGCTA AAAAGAAAAG ATTCTCACCT TCTCCTATTT CCTCCTCTAC ATTTTCGTTC 
TCACCCAAAT CTAGGGTCAC TTCTTCAAAC TCTTCTGGCA ATGAAGACGG TAACCTAATG 
AATACACCTT CTACGGTTTC CACTGATTAT TTGCCACAAC ACCCTCACAG AACATCGTCT 
TTGCCAAGAC CTAATTCCAA TCTCTTTCAC GCAAGTAATA GTAACCTATC CCGAGCAAAT 
GAGCCCCCAA GGGCCGAAAA TTTATCAGAT AATATACCAC CCAAGGTCGC TCCATTTGGC 
TATCCAATAC AAAGAACCTC TATTAAAAAA TCCTTTTTGA ATGCTTCTTG TACGTTATGT 
GACGAGCCTA TTTCTAACAG AAGAAAGGGA GAGAAAATTA TAGAGCTTGC ATGTGGCCAC 
TTAAGTCACC AAGAATGTCT TATTATCTCT TTTGGCACCA CTTCAAAGGC AGACGTTCGT 
GCGCTATTTC CTTTTTGTAC CAAATGTAAA AAAGATACTA ACAAAGCCGT TCAATGCATT 
CCAGAAAATG ATGAACTAAA GGATATTCTA ATTTCTGATT TTTTGATTCA TAAGATTCCT 
GATTCTGAGT TATCAATCAC ACCTCAGTCC CGCTTTCCTC CTTATTCACC ACTCTTGCCT 
CCTTTTGGGT TATCCTATAC ACCTGTTGAA AGACAAACGA TATATTCTCA AGCTCCAAGT 
CTAAACCCAA ATCTCATATT GGCTGCACCC CCCAAGGAAA GAAACCAAAT TCCACAAAAA 
AAATCAAACT ATACATTTTT ACATTCACCC CTGGGGCACA GAAGAATTCC GTCCGGAGCA 
AACTCTATCT TAGCAGACAC CTCTGTAGCG TTGTCAGCTA ATGATTCTAT TTCTGCTGTT 
TCCAATTCGG TAAGAGCAAA GGATGACGAA ACCAAAACAA CGTTGCCGCT GTTAAGGTCA 
TATTTTATTC AAATTCTTTT GAACAATTTC CAGGAAGAAT TGCAGGATTG GAGAATAGAC 
GGGGACTATG GATTACTAAG GTTGGTAGAC AAATTGATGA TTTCCAAAGA TGGTCAGAGA 
TATATACAAT GCTGGTGTTT CTTATTTGAA GACGCATTTG TAATAGCAGA AGTGGATAAC 
GATGTTGATG TTTTGGAAAT TAGACTAAAG AATTTAGAAG TATTTACACC TATTGCCAAC 
TTGAGAATGA CTACACTCGA AGCTTCAGTA CTCAAATGCA CCTTAAATAA ACAACATTGC 
GCCGATTTAT CAGATCTTTA CATTGTTCAG AATATAAATT CTGACGAAAG CACAACTGTA 
CAGAAATGGA TATCAGGTAT ATTGAATCAG GATTTTGTAT TCAATGAGGA CAATATCACT 
TCGACCCTGC CTATTCTTCC CATTATAAAG AACTTTTCAA AAGATGTTGG TAATGGTAGG 
CACGAGACGA GTACCTTTCT AGGTTTAATC AATCCTAACA AAGTTGTTGA AGTTGGAAAT 
GTGCACGATA ATGATACTGT AATCATAAGG AGGGGATTCA CCTTAAATTC AGGAGAATGT 
TCTAGGCAGA GTACTGTCGA CAGTATACAA TCTGTTCTAA CCACGATAAG CTCAATTCTT 
TCCCTTAAAC GAGAAAAACC TGATAATTTG GCAATAATCT TACAGATCGA TTTTACGAAA 
TTGAAGGAAG AAGACAGTTT AATTGTTGTT TATAACAGTC TAAAAGCTTT AACCATTAAA 
TTTGCGCGTT TGCAGTTTTG TTTCGTTGAT CGAAATAATT ATGTTCTGGA CTATGGATCG 
GTATTACACA AGATAGATTC ACTAGATTCC ATCTCAAATC TCAAATCAAA GAGTTCCTCG 
ACACAATTTT CACCTATTTG GTTGAAAAAT ACTCTATATC CCGAAAATAT TCATGAACAT 
TTGGGTATTG TTGCTGTATC AAATAGTAAT ATGGAAGCAA AAAAATCCAT ACTATTTCAA 
GATTACAGAT GCTTTACAAG TTTTGGAAGA AGAAGGCCCA ATGAATTGAA GATTAAGGTG 
GGCTATTTGA ACGTTGACTA CAGTGATAAA ATTGATGAAC TAGTCGAGGC CAGCTCCTGG 
ACTTTTGTTT TAGAAACTCT TTGCTACAGT TTCGGTCTAA GTTTTGATGA ACATGATGAC 
GATGACGAAG AGGATAATGA TGATTCGACC GATAATGAAC TTGATAATAG TTCAGGATCA 
CTGTCGGATG CTGAATCTAC AACTACTATT CATATTGATT CTCCATTTGA TAATGAAAAT 
GCTACCGCAA ATATGGTGAA TGACAGAAAC CTTCTCACTG AGGGTGAACA TAGCAATATA 
GAAAACTTAG AAACTGTCGC TTCTTCAGTA CAGCCAGCTC TGATTCCTAA TATTAGATTT 
TCACTTCATT CTGAGGAGGA AGGTACTAAT GAAAATGAAA ATGAAAATGA TATGCCAGTA 
TTATTACTTA GTGATATGGA TAAAGGAATC GATGGCATAA CCAGACGCAG TTCATTCTCG 
AGTCTTATAG AGAGCGGTAA TAACAACTGT CCCCTCCATA TGGATTATAT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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STE5 cDNA ORF clone, Saccharomyces cerevisiae S288C

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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