DNF2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	DNF2
Entrez Gene ID	851667
Full Name	aminophospholipid-translocating P4-type ATPase DNF2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180401.1	NP_010378.1	aminophospholipid-translocating P4-type ATPase DNF2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP10A	NP_077816.1
Pan troglodytes (chimpanzee)	ATP10A	XP_510255.2
Canis lupus familiaris (dog)	ATP10A	XP_545808.4
Bos taurus (cattle)	ATP10A	XP_005221762.1
	ATP10A	XP_001108767.1
Magnaporthe oryzae (rice blast fungus)	MGG_02767	XP_003720981.1
Neurospora crassa	NCU07443	XP_964630.1
Danio rerio (zebrafish)	atp10a	XP_695556.2
Arabidopsis thaliana (thale cress)	ALA1	NP_568146.1
Schizosaccharomyces pombe (fission yeast)	SPAC24B11.12c	NP_592849.1
Mus musculus (house mouse)	Atp10a	NP_033858.2
Rattus norvegicus (Norway rat)	Atp10a	NP_001135407.1
Gallus gallus (chicken)	ATP10A	XP_416881.3
Saccharomyces cerevisiae (baker's yeast)	DNF1	NP_011093.3
Saccharomyces cerevisiae (baker's yeast)	DNF2	NP_010378.1
Kluyveromyces lactis	KLLA0C17644g	XP_452988.1
Eremothecium gossypii	AGOS_AGR120C	NP_986786.2
Oryza sativa (rice)	Os01g0277600	NP_001042736.2
Xenopus tropicalis (tropical clawed frog)	atp10a	XP_002931688.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Lem3-Dnf1/Dnf2 complex regulates the polar Cdc42 dynamics and phosphatidylserine distribution.
Title: Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell?polarity.

aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae
Title: The putative aminophospholipid translocases, DNF1 and DNF2, are not required for 7-nitrobenz-2-oxa-1,3-diazol-4-yl-phosphatidylserine flip across the plasma membrane of Saccharomyces cerevisiae.

The ELM pathway for lyso-PtdEtn requires the action of Dnf1p and Dnf2p and their requisite beta-subunit, Lem3p, for the active uptake of lyso-PtdEtn. In addition, Ale1p, mediates the acylation of the imported lysolipid to form PtdEtn.
Title: Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation.

The following DNF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01183	NM_001180401.1 Latest version!	Saccharomyces cerevisiae S288C aminophospholipid-translocating P4-type ATPase DNF2 (DNF2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNF2 ( NM_001180401.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010378.1 Saccharomyces cerevisiae S288C aminophospholipid-translocating P4-type ATPase DNF2 (DNF2), partial mRNA.

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CloneID	OSi01183
Clone ID Related Accession (Same CDS sequence)	NM_001180401.1
Accession Version	NM_001180401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	aminophospholipid-translocating P4-type ATPase DNF2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAAGTC CCTCCAAACC CACTTCTCCC TTCGTGGATG ATATTGAGCA TGAATCGGGA 
TCAGCATCTA ATGGTCTGTC GTCCATGTCA CCATTTGACG ATAGTTTTCA ATTTGAAAAA 
CCCAGTAGTG CGCATGGAAA TATTGAAGTA GCGAAAACCG GCGGTTCTGT TTTGAAGCGA 
CAATCTAAGC CAATGAAAGA TATTAGCACA CCCGATCTCT CAAAAGTTAC TTTTGATGGA 
ATCGATGATT ATAGTAACGA TAATGATATT AATGATGATG ATGAACTCAA TGGTAAAAAA 
ACCGAGATAC ATGAACACGA AAATGAGGTG GATGATGACC TCCATTCGTT CCAGGCAACA 
CCTATGCCTA ATACTGGAGG CTTTGAGGAT GTTGAGTTGG ATAACAATGA AGGTAGCAAC 
AATGATTCCC AGGCAGATCA TAAGTTGAAA AGAGTACGTT TTGGTACAAG AAGAAATAAG 
TCAGGTAGGA TAGATATAAA TAGGTCAAAA ACATTGAAAT GGGCCAAGAA GAACTTCCAC 
AATGCTATTG ATGAATTCAG TACAAAAGAA GACAGTCTCG AAAATTCAGC CTTGCAAAAT 
AGGTCTGATG AGTTGAGAAC AGTTTACTAT AATTTACCCT TGCCGGAGGA CATGCTTGAC 
GAGGACGGCT TACCGTTGGC AGTTTACCCG CGTAATAAAA TCAGGACTAC GAAGTATACT 
CCATTAACAT TTTTTCCCAA GAACATTCTT TTCCAATTTC ATAATTTTGC CAATATTTAC 
TTTTTAATAC TGTTGATTCT AGGTGCTTTT CAAATATTTG GTGTGACAAA TCCAGGTTTT 
GCATCCGTTC CCCTAATTGT CATCGTAATT ATCACTGCTA TTAAGGATGG TATCGAAGAT 
TCCAGAAGGA CTGTATTGGA TTTAGAAGTT AATAATACAA GAACACATAT CTTAAGCGGG 
GTGAAGAATG AAAATGTTGC TGTTGATAAC GTTTCCTTAT GGAGGCGTTT CAAGAAAGCG 
AATACAAGGG CATTGATTAA AATTTTTGAG TATTTTTCAG AAAACCTCAC TGCTGCTGGT 
AGAGAAAAGA AGTTACAGAA AAAAAGGGAA GAATTACGAA GGAAGAGGAA CTCGAGAAGC 
TTTGGGCCAC GTGGGTCTCT GGATTCAATT GGTAGTTACA GAATGTCTGC CGACTTTGGT 
CGTCCCTCTT TGGATTATGA GAACCTGAAT CAAACAATGT CACAGGCTAA CAGGTATAAC 
GATGGTGAAA ATTTAGTTGA TCGAACGCTA CAGCCAAACC CTGAATGTAG GTTTGCCAAG 
GATTACTGGA AAAATGTTAA AGTTGGTGAT ATTGTTCGTG TTCACAATAA CGATGAAATT 
CCTGCTGATA TGATCTTATT ATCGACATCA GATGTTGATG GCGCTTGTTA TGTGGAAACC 
AAAAATTTGG ATGGTGAAAC AAATCTAAAG GTCCGTCAAT CATTAAAATG TAGCAAGATT 
ATTAAGAGTT CAAGGGATAT TACCAGGACG AAGTTTTGGG TTGAAAGTGA AGGCCCACAT 
GCCAACTTGT ATTCTTATCA AGGTAATTTC AAATGGCAAG ATACACAAAA TGGAAACATC 
AGAAATGAGC CTGTAAATAT CAATAACTTA TTATTGAGAG GTTGTACCTT GAGAAACACT 
AAGTGGGCCA TGGGTATGGT TATTTTTACT GGTGACGATA CCAAGATTAT GATAAACGCT 
GGTGTCACTC CAACCAAAAA ATCCAGAATT TCAAGAGAGT TGAATTTTTC TGTGATTTTG 
AATTTTGTAC TATTGTTTAT CCTATGTTTC ACTGCTGGTA TTGTTAACGG TGTTTATTAT 
AAACAGAAAC CTCGATCGAG AGATTATTTT GAATTTGGTA CCATTGGAGG TTCTGCCTCA 
ACTAACGGTT TTGTTTCTTT TTGGGTGGCG GTTATTCTTT ATCAGTCATT GGTTCCGATC 
TCTTTATATA TTTCTGTGGA AATTATTAAG ACAGCACAGG CTATTTTCAT TTACACTGAT 
GTTTTGTTGT ATAATGCCAA GCTAGACTAT CCATGTACAC CAAAGTCGTG GAACATCTCC 
GATGATTTGG GTCAAATTGA GTATATTTTT TCAGATAAAA CAGGCACCTT GACACAAAAT 
GTGATGGAAT TCAAAAAATG TACTATTAAC GGTGTGTCTT ATGGACGTGC CTATACGGAG 
GCACTAGCAG GCCTGAGGAA GAGACAAGGT GTTGATGTCG AATCGGAAGG TCGTCGCGAA 
AAGGAAGAGA TAGCAAAAGA TAGGGAAACA ATGATCGACG AATTAAGATC AATGAGTGAT 
AATACACAGT TTTGTCCGGA GGATTTAACT TTTGTATCTA AGGAAATTGT GGAAGACTTG 
AAAGGATCCA GTGGCGACCA TCAACAAAAG TGCTGTGAAC ACTTTTTGCT GGCGCTCGCA 
TTATGTCATT CTGTCCTTGT GGAGCCAAAT AAAGATGATC CTAAAAAACT GGACATCAAG 
GCTCAATCAC CTGATGAGTC TGCCTTAGTT TCCACCGCCA GACAATTAGG GTACAGTTTC 
GTTGGTAGTT CAAAAAGTGG ATTGATAGTA GAGATCCAGG GTGTTCAAAA GGAGTTCCAA 
GTTCTGAACG TACTTGAATT CAATTCGTCG AGAAAAAGGA TGAGTTGCAT TATCAAGATC 
CCTGGCTCTA CACCAAAGGA TGAACCTAAA GCACTGTTAA TATGTAAAGG TGCGGATTCC 
GTTATCTATT CAAGATTAGA TCGCACTCAA AACGACGCTA CTTTGTTGGA AAAGACGGCC 
CTCCATTTGG AAGAATATGC CACAGAAGGT TTAAGAACAT TATGTTTGGC TCAAAGAGAG 
CTCACGTGGT CCGAGTATGA ACGTTGGGTT AAGACTTATG ATGTGGCCGC TGCATCCGTT 
ACTAATAGGG AGGAAGAGTT GGACAAAGTT ACTGACGTTA TTGAACGTGA GCTTATTTTG 
CTTGGTGGTA CGGCCATTGA GGATCGTTTA CAAGATGGTG TTCCTGATTC GATTGCCCTC 
TTGGCCGAGG CCGGTATTAA GTTATGGGTC CTAACGGGTG ATAAAGTGGA AACTGCCATT 
AACATTGGTT TTTCCTGCAA TGTTTTGAAC AATGATATGG AATTATTGGT TGTGAAGGCT 
TCCGGCGAAG ATGTAGAAGA ATTTGGTAGT GACCCTATTC AAGTGGTTAA CAATTTGGTG 
ACAAAATACT TAAGGGAAAA ATTTGGCATG AGTGGATCCG AGGAGGAGTT AAAAGAAGCT 
AAAAGAGAGC ATGGTTTGCC ACAGGGAAAT TTTGCTGTCA TTATCGATGG CGATGCGCTA 
AAAGTTGCTT TGAATGGTGA AGAAATGAGA CGCAAGTTCT TGCTACTGTG TAAGAATTGT 
AAAGCTGTTT TGTGTTGCAG AGTTTCTCCA GCACAAAAGG CTGCAGTGGT CAAGTTGGTA 
AAAAAGACCT TAGACGTTAT GACCTTGGCC ATTGGTGATG GTTCGAATGA TGTAGCCATG 
ATTCAATCGG CGGACGTTGG TGTTGGTATC GCGGGTGAAG AAGGCAGACA AGCTGTCATG 
TGTTCTGATT ATGCCATCGG GCAGTTCAGA TATGTGACAA GACTGGTATT GGTGCATGGT 
AAATGGTGTT ATAAGAGACT GGCAGAGATG ATCCCACAGT TTTTCTACAA GAACGTTATT 
TTTACGCTAT CCCTGTTCTG GTATGGTATT TACAATAATT TCGATGGGTC GTATTTGTTT 
GAGTATACGT ACTTGACATT CTACAATCTT GCATTCACAT CCGTACCTGT TATTTTACTA 
GCAGTACTCG ACCAAGATGT TTCCGACACC GTTTCGATGC TGGTGCCACA GTTGTACCGT 
GTGGGTATCC TAAGAAAAGA ATGGAACCAA ACCAAATTTC TTTGGTACAT GTTGGATGGT 
GTATACCAAT CTGTGATATG CTTCTTTTTC CCCTATTTGG CGTACCATAA AAACATGGTT 
GTAACTGAAA ATGGCCTTGG ACTAGACCAC CGTTACTTTG TCGGTGTCTT CGTTACAGCA 
ATTGCAGTCA CTTCATGTAA CTTTTACGTC TTCATGGAAC AGTATAGATG GGACTGGTTC 
TGTGGCCTAT TCATATGTCT ATCACTGGCC GTGTTTTACG GCTGGACGGG TATATGGACA 
AGCTCGTCGT CAAGTAACGA ATTTTACAAG GGTGCGGCGC GTGTATTCGC ACAACCTGCC 
TATTGGGCAG TACTTTTCGT TGGCGTGCTG TTCTGCCTAC TGCCAAGATT CACCATTGAC 
TGTATCAGAA AGATATTCTA TCCGAAAGAT ATAGAGATCG TTAGAGAAAT GTGGTTACGC 
GGTGATTTTG ACCTCTACCC ACAAGGCTAC GACCCTACGG ACCCTAGCAG GCCCAGAATA 
AACGAAATAC GTCCCCTTAC AGATTTCAAA GAGCCCATTT CACTGGACAC TCATTTTGAT 
GGTGTCAGCC ACTCACAAGA GACCATAGTC ACAGAGGAAA TACCAATGAG TATACTCAAT 
GGTGAGCAAG GCTCGCGCAA GGGGTACCGT GTCTCTACCA CTCTGGAACG CCGTGACCAG 
CTCAGCCCCG TTACAACAAC GAACAACTTG CCTAGAAGAT CAATGGCCAG TGCCCGCGGA 
AATAAGCTCA GAACTTCGCT GGATCGCACA AGAGAAGAGA TGCTTGCCAA CCACCAACTG 
GACACAAGAT ACTCCGTGGA AAGAGCACGT GCCTCGCTGG ATCTGCCGGG TATCAATCAT 
GCAGAGACGC TGCTCAGCCA GCGCAGCAGG GACCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010378.1
CDS	1..4839
Translation	MSSPSKPTSPFVDDIEHESGSASNGLSSMSPFDDSFQFEKPSSAHGNIEVAKTGGSVLKRQSKPMKDISTPDLSKVTFDGIDDYSNDNDINDDDELNGKKTEIHEHENEVDDDLHSFQATPMPNTGGFEDVELDNNEGSNNDSQADHKLKRVRFGTRRNKSGRIDINRSKTLKWAKKNFHNAIDEFSTKEDSLENSALQNRSDELRTVYYNLPLPEDMLDEDGLPLAVYPRNKIRTTKYTPLTFFPKNILFQFHNFANIYFLILLILGAFQIFGVTNPGFASVPLIVIVIITAIKDGIEDSRRTVLDLEVNNTRTHILSGVKNENVAVDNVSLWRRFKKANTRALIKIFEYFSENLTAAGREKKLQKKREELRRKRNSRSFGPRGSLDSIGSYRMSADFGRPSLDYENLNQTMSQANRYNDGENLVDRTLQPNPECRFAKDYWKNVKVGDIVRVHNNDEIPADMILLSTSDVDGACYVETKNLDGETNLKVRQSLKCSKIIKSSRDITRTKFWVESEGPHANLYSYQGNFKWQDTQNGNIRNEPVNINNLLLRGCTLRNTKWAMGMVIFTGDDTKIMINAGVTPTKKSRISRELNFSVILNFVLLFILCFTAGIVNGVYYKQKPRSRDYFEFGTIGGSASTNGFVSFWVAVILYQSLVPISLYISVEIIKTAQAIFIYTDVLLYNAKLDYPCTPKSWNISDDLGQIEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAYTEALAGLRKRQGVDVESEGRREKEEIAKDRETMIDELRSMSDNTQFCPEDLTFVSKEIVEDLKGSSGDHQQKCCEHFLLALALCHSVLVEPNKDDPKKLDIKAQSPDESALVSTARQLGYSFVGSSKSGLIVEIQGVQKEFQVLNVLEFNSSRKRMSCIIKIPGSTPKDEPKALLICKGADSVIYSRLDRTQNDATLLEKTALHLEEYATEGLRTLCLAQRELTWSEYERWVKTYDVAAASVTNREEELDKVTDVIERELILLGGTAIEDRLQDGVPDSIALLAEAGIKLWVLTGDKVETAINIGFSCNVLNNDMELLVVKASGEDVEEFGSDPIQVVNNLVTKYLREKFGMSGSEEELKEAKREHGLPQGNFAVIIDGDALKVALNGEEMRRKFLLLCKNCKAVLCCRVSPAQKAAVVKLVKKTLDVMTLAIGDGSNDVAMIQSADVGVGIAGEEGRQAVMCSDYAIGQFRYVTRLVLVHGKWCYKRLAEMIPQFFYKNVIFTLSLFWYGIYNNFDGSYLFEYTYLTFYNLAFTSVPVILLAVLDQDVSDTVSMLVPQLYRVGILRKEWNQTKFLWYMLDGVYQSVICFFFPYLAYHKNMVVTENGLGLDHRYFVGVFVTAIAVTSCNFYVFMEQYRWDWFCGLFICLSLAVFYGWTGIWTSSSSSNEFYKGAARVFAQPAYWAVLFVGVLFCLLPRFTIDCIRKIFYPKDIEIVREMWLRGDFDLYPQGYDPTDPSRPRINEIRPLTDFKEPISLDTHFDGVSHSQETIVTEEIPMSILNGEQGSRKGYRVSTTLERRDQLSPVTTTNNLPRRSMASARGNKLRTSLDRTREEMLANHQLDTRYSVERARASLDLPGINHAETLLSQRSRDR

Target ORF information:

RefSeq Version	NM_001180401.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C aminophospholipid-translocating P4-type ATPase DNF2 (DNF2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180401.1

ORF Insert Sequence:

ATGTCAAGTC CCTCCAAACC CACTTCTCCC TTCGTGGATG ATATTGAGCA TGAATCGGGA 
TCAGCATCTA ATGGTCTGTC GTCCATGTCA CCATTTGACG ATAGTTTTCA ATTTGAAAAA 
CCCAGTAGTG CGCATGGAAA TATTGAAGTA GCGAAAACCG GCGGTTCTGT TTTGAAGCGA 
CAATCTAAGC CAATGAAAGA TATTAGCACA CCCGATCTCT CAAAAGTTAC TTTTGATGGA 
ATCGATGATT ATAGTAACGA TAATGATATT AATGATGATG ATGAACTCAA TGGTAAAAAA 
ACCGAGATAC ATGAACACGA AAATGAGGTG GATGATGACC TCCATTCGTT CCAGGCAACA 
CCTATGCCTA ATACTGGAGG CTTTGAGGAT GTTGAGTTGG ATAACAATGA AGGTAGCAAC 
AATGATTCCC AGGCAGATCA TAAGTTGAAA AGAGTACGTT TTGGTACAAG AAGAAATAAG 
TCAGGTAGGA TAGATATAAA TAGGTCAAAA ACATTGAAAT GGGCCAAGAA GAACTTCCAC 
AATGCTATTG ATGAATTCAG TACAAAAGAA GACAGTCTCG AAAATTCAGC CTTGCAAAAT 
AGGTCTGATG AGTTGAGAAC AGTTTACTAT AATTTACCCT TGCCGGAGGA CATGCTTGAC 
GAGGACGGCT TACCGTTGGC AGTTTACCCG CGTAATAAAA TCAGGACTAC GAAGTATACT 
CCATTAACAT TTTTTCCCAA GAACATTCTT TTCCAATTTC ATAATTTTGC CAATATTTAC 
TTTTTAATAC TGTTGATTCT AGGTGCTTTT CAAATATTTG GTGTGACAAA TCCAGGTTTT 
GCATCCGTTC CCCTAATTGT CATCGTAATT ATCACTGCTA TTAAGGATGG TATCGAAGAT 
TCCAGAAGGA CTGTATTGGA TTTAGAAGTT AATAATACAA GAACACATAT CTTAAGCGGG 
GTGAAGAATG AAAATGTTGC TGTTGATAAC GTTTCCTTAT GGAGGCGTTT CAAGAAAGCG 
AATACAAGGG CATTGATTAA AATTTTTGAG TATTTTTCAG AAAACCTCAC TGCTGCTGGT 
AGAGAAAAGA AGTTACAGAA AAAAAGGGAA GAATTACGAA GGAAGAGGAA CTCGAGAAGC 
TTTGGGCCAC GTGGGTCTCT GGATTCAATT GGTAGTTACA GAATGTCTGC CGACTTTGGT 
CGTCCCTCTT TGGATTATGA GAACCTGAAT CAAACAATGT CACAGGCTAA CAGGTATAAC 
GATGGTGAAA ATTTAGTTGA TCGAACGCTA CAGCCAAACC CTGAATGTAG GTTTGCCAAG 
GATTACTGGA AAAATGTTAA AGTTGGTGAT ATTGTTCGTG TTCACAATAA CGATGAAATT 
CCTGCTGATA TGATCTTATT ATCGACATCA GATGTTGATG GCGCTTGTTA TGTGGAAACC 
AAAAATTTGG ATGGTGAAAC AAATCTAAAG GTCCGTCAAT CATTAAAATG TAGCAAGATT 
ATTAAGAGTT CAAGGGATAT TACCAGGACG AAGTTTTGGG TTGAAAGTGA AGGCCCACAT 
GCCAACTTGT ATTCTTATCA AGGTAATTTC AAATGGCAAG ATACACAAAA TGGAAACATC 
AGAAATGAGC CTGTAAATAT CAATAACTTA TTATTGAGAG GTTGTACCTT GAGAAACACT 
AAGTGGGCCA TGGGTATGGT TATTTTTACT GGTGACGATA CCAAGATTAT GATAAACGCT 
GGTGTCACTC CAACCAAAAA ATCCAGAATT TCAAGAGAGT TGAATTTTTC TGTGATTTTG 
AATTTTGTAC TATTGTTTAT CCTATGTTTC ACTGCTGGTA TTGTTAACGG TGTTTATTAT 
AAACAGAAAC CTCGATCGAG AGATTATTTT GAATTTGGTA CCATTGGAGG TTCTGCCTCA 
ACTAACGGTT TTGTTTCTTT TTGGGTGGCG GTTATTCTTT ATCAGTCATT GGTTCCGATC 
TCTTTATATA TTTCTGTGGA AATTATTAAG ACAGCACAGG CTATTTTCAT TTACACTGAT 
GTTTTGTTGT ATAATGCCAA GCTAGACTAT CCATGTACAC CAAAGTCGTG GAACATCTCC 
GATGATTTGG GTCAAATTGA GTATATTTTT TCAGATAAAA CAGGCACCTT GACACAAAAT 
GTGATGGAAT TCAAAAAATG TACTATTAAC GGTGTGTCTT ATGGACGTGC CTATACGGAG 
GCACTAGCAG GCCTGAGGAA GAGACAAGGT GTTGATGTCG AATCGGAAGG TCGTCGCGAA 
AAGGAAGAGA TAGCAAAAGA TAGGGAAACA ATGATCGACG AATTAAGATC AATGAGTGAT 
AATACACAGT TTTGTCCGGA GGATTTAACT TTTGTATCTA AGGAAATTGT GGAAGACTTG 
AAAGGATCCA GTGGCGACCA TCAACAAAAG TGCTGTGAAC ACTTTTTGCT GGCGCTCGCA 
TTATGTCATT CTGTCCTTGT GGAGCCAAAT AAAGATGATC CTAAAAAACT GGACATCAAG 
GCTCAATCAC CTGATGAGTC TGCCTTAGTT TCCACCGCCA GACAATTAGG GTACAGTTTC 
GTTGGTAGTT CAAAAAGTGG ATTGATAGTA GAGATCCAGG GTGTTCAAAA GGAGTTCCAA 
GTTCTGAACG TACTTGAATT CAATTCGTCG AGAAAAAGGA TGAGTTGCAT TATCAAGATC 
CCTGGCTCTA CACCAAAGGA TGAACCTAAA GCACTGTTAA TATGTAAAGG TGCGGATTCC 
GTTATCTATT CAAGATTAGA TCGCACTCAA AACGACGCTA CTTTGTTGGA AAAGACGGCC 
CTCCATTTGG AAGAATATGC CACAGAAGGT TTAAGAACAT TATGTTTGGC TCAAAGAGAG 
CTCACGTGGT CCGAGTATGA ACGTTGGGTT AAGACTTATG ATGTGGCCGC TGCATCCGTT 
ACTAATAGGG AGGAAGAGTT GGACAAAGTT ACTGACGTTA TTGAACGTGA GCTTATTTTG 
CTTGGTGGTA CGGCCATTGA GGATCGTTTA CAAGATGGTG TTCCTGATTC GATTGCCCTC 
TTGGCCGAGG CCGGTATTAA GTTATGGGTC CTAACGGGTG ATAAAGTGGA AACTGCCATT 
AACATTGGTT TTTCCTGCAA TGTTTTGAAC AATGATATGG AATTATTGGT TGTGAAGGCT 
TCCGGCGAAG ATGTAGAAGA ATTTGGTAGT GACCCTATTC AAGTGGTTAA CAATTTGGTG 
ACAAAATACT TAAGGGAAAA ATTTGGCATG AGTGGATCCG AGGAGGAGTT AAAAGAAGCT 
AAAAGAGAGC ATGGTTTGCC ACAGGGAAAT TTTGCTGTCA TTATCGATGG CGATGCGCTA 
AAAGTTGCTT TGAATGGTGA AGAAATGAGA CGCAAGTTCT TGCTACTGTG TAAGAATTGT 
AAAGCTGTTT TGTGTTGCAG AGTTTCTCCA GCACAAAAGG CTGCAGTGGT CAAGTTGGTA 
AAAAAGACCT TAGACGTTAT GACCTTGGCC ATTGGTGATG GTTCGAATGA TGTAGCCATG 
ATTCAATCGG CGGACGTTGG TGTTGGTATC GCGGGTGAAG AAGGCAGACA AGCTGTCATG 
TGTTCTGATT ATGCCATCGG GCAGTTCAGA TATGTGACAA GACTGGTATT GGTGCATGGT 
AAATGGTGTT ATAAGAGACT GGCAGAGATG ATCCCACAGT TTTTCTACAA GAACGTTATT 
TTTACGCTAT CCCTGTTCTG GTATGGTATT TACAATAATT TCGATGGGTC GTATTTGTTT 
GAGTATACGT ACTTGACATT CTACAATCTT GCATTCACAT CCGTACCTGT TATTTTACTA 
GCAGTACTCG ACCAAGATGT TTCCGACACC GTTTCGATGC TGGTGCCACA GTTGTACCGT 
GTGGGTATCC TAAGAAAAGA ATGGAACCAA ACCAAATTTC TTTGGTACAT GTTGGATGGT 
GTATACCAAT CTGTGATATG CTTCTTTTTC CCCTATTTGG CGTACCATAA AAACATGGTT 
GTAACTGAAA ATGGCCTTGG ACTAGACCAC CGTTACTTTG TCGGTGTCTT CGTTACAGCA 
ATTGCAGTCA CTTCATGTAA CTTTTACGTC TTCATGGAAC AGTATAGATG GGACTGGTTC 
TGTGGCCTAT TCATATGTCT ATCACTGGCC GTGTTTTACG GCTGGACGGG TATATGGACA 
AGCTCGTCGT CAAGTAACGA ATTTTACAAG GGTGCGGCGC GTGTATTCGC ACAACCTGCC 
TATTGGGCAG TACTTTTCGT TGGCGTGCTG TTCTGCCTAC TGCCAAGATT CACCATTGAC 
TGTATCAGAA AGATATTCTA TCCGAAAGAT ATAGAGATCG TTAGAGAAAT GTGGTTACGC 
GGTGATTTTG ACCTCTACCC ACAAGGCTAC GACCCTACGG ACCCTAGCAG GCCCAGAATA 
AACGAAATAC GTCCCCTTAC AGATTTCAAA GAGCCCATTT CACTGGACAC TCATTTTGAT 
GGTGTCAGCC ACTCACAAGA GACCATAGTC ACAGAGGAAA TACCAATGAG TATACTCAAT 
GGTGAGCAAG GCTCGCGCAA GGGGTACCGT GTCTCTACCA CTCTGGAACG CCGTGACCAG 
CTCAGCCCCG TTACAACAAC GAACAACTTG CCTAGAAGAT CAATGGCCAG TGCCCGCGGA 
AATAAGCTCA GAACTTCGCT GGATCGCACA AGAGAAGAGA TGCTTGCCAA CCACCAACTG 
GACACAAGAT ACTCCGTGGA AAGAGCACGT GCCTCGCTGG ATCTGCCGGG TATCAATCAT 
GCAGAGACGC TGCTCAGCCA GCGCAGCAGG GACCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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DNF2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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