FUN12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	FUN12
Entrez Gene ID	851196
Full Name	translation initiation factor eIF5B
Synonyms	yIF2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178180.1	NP_009365.1	translation initiation factor eIF5B

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_ADR373W	NP_984469.2
Schizosaccharomyces pombe (fission yeast)	SPAC56F8.03	NP_593218.1
Saccharomyces cerevisiae (baker's yeast)	FUN12	NP_009365.1
Kluyveromyces lactis	KLLA0F23265g	XP_456116.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Bussey H, Kaback DB, Zhong W, Vo DT, Clark MW, Fortin N, Hall J, Ouellette BF, Keng T, Barton AB
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The structures of two isolated forms of yeast eIF5B and of the eIF5B-eIF1A complex (eIF1A and eIF5B do not contain the respective N-terminal domains) are reported.
Title: X-ray structures of eIF5B and the eIF5B-eIF1A complex: the conformational flexibility of eIF5B is restricted on the ribosome by interaction with eIF1A.

propose that recruitment of 60S subunits promotes GTP hydrolysis by Fun12, leading to structural rearrangements within the pre-40S particle that bring Nob1 and the pre-rRNA cleavage site together
Title: Proofreading of pre-40S ribosome maturation by a translation initiation factor and 60S subunits.

helix H12 is not critical for eIF5B binding to the ribosome or for GTP hydrolysis with purified ribosomes; however, the length and rigidity of helix H12 are important for formation of stable 80S ribosomal complexes.
Title: Structural integrity of {alpha}-helix H12 in translation initiation factor eIF5B is critical for 80S complex stability.

A mutation in helix h5 of the 18S rRNA in the 40S ribosomal subunit and intragenic mutations in domain II of eIF5B suppress the toxic effects associated with expression of the eIF5B-H480I GTPase-deficient mutant in yeast.
Title: rRNA suppressor of a eukaryotic translation initiation factor 5B/initiation factor 2 mutant reveals a binding site for translational GTPases on the small ribosomal subunit.

The following FUN12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FUN12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00783	NM_001178180.1 Latest version!	Saccharomyces cerevisiae S288C translation initiation factor eIF5B (FUN12), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FUN12 ( NM_001178180.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009365.1 Saccharomyces cerevisiae S288C translation initiation factor eIF5B (FUN12), partial mRNA.

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CloneID	OSi00783
Clone ID Related Accession (Same CDS sequence)	NM_001178180.1
Accession Version	NM_001178180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	translation initiation factor eIF5B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001133). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCGAAAA AGAGTAAAAA GAACCAACAG AACTACTGGG ATGAGGAATT CGAAGAAGAC 
GCCGCCCAGA ACGAAGAAAT CAGTGCCACG CCAACTCCAA ATCCAGAAAG CAGCGCAGGT 
GCAGATGACA CTTCCAGAGA AGCAAGTGCA AGTGCTGAAG GTGCTGAGGC CATTGAAGGC 
GACTTCATGT CTACTTTGAA GCAATCGAAG AAGAAGCAAG AAAAGAAGGT TATTGAAGAG 
AAGAAGGATG GTAAGCCTAT ACTAAAGTCC AAGAAGGAAA AGGAGAAGGA AAAAAAGGAA 
AAGGAGAAGC AGAAGAAGAA AGAACAAGCT GCCAGGAAGA AGGCCCAACA GCAAGCTCAA 
AAGGAGAAGA ACAAGGAGTT GAACAAGCAA AATGTTGAAA AAGCTGCTGC TGAGAAGGCT 
GCTGCTGAGA AATCCCAAAA ATCTAAAGGT GAAAGTGATA AACCAAGTGC TAGTGCTAAG 
AAGCCAGCCA AGAAAGTACC TGCCGGTTTG GCTGCTTTGA GACGTCAATT AGAATTGAAG 
AAACAACTTG AAGAACAAGA AAAGTTGGAA AGAGAGGAAG AAGAAAGATT GGAGAAAGAA 
GAGGAGGAAA GATTGGCCAA CGAAGAAAAA ATGAAGGAAG AAGCTAAAGC AGCTAAAAAG 
GAAAAGGAGA AGGCAAAGCG TGAAAAACGA AAGGCTGAAG GTAAGCTATT GACCAGAAAG 
CAAAAAGAAG AAAAGAAATT ATTGGAAAGA AGACGTGCCG CTTTATTGTC TTCCGGTAAC 
GTCAAAGTTG CCGGTCTGGC CAAGAAGGAT GGAGAAGAAA ACAAACCAAA GAAGGTTGTT 
TACAGCAAGA AGAAGAAGAG AACAACCCAG GAAAACGCCT CCGAAGCCAT TAAATCTGAC 
TCTAAGAAAG ACTCGGAAGT TGTACCTGAT GACGAACTCA AAGAATCCGA AGATGTTTTG 
ATTGATGATT GGGAAAATTT GGCTCTTGGT GATGATGACG AGGAGGGAAC CAACGAAGAA 
ACGCAAGAAT CCACCGCAAG CCATGAAAAT GAAGACCAAA ATCAAGGCGA AGAAGAAGAA 
GAAGGAGAAG AAGAAGAAGA AGAAGAAGAA GAAAGAGCAC ATGTGCATGA AGTTGCCAAA 
AGCACACCAG CAGCTACACC AGCAGCTACT CCAACTCCAT CCAGCGCTTC TCCAAACAAA 
AAAGATCTTC GTTCCCCAAT TTGTTGTATT TTGGGTCATG TCGATACCGG TAAGACTAAA 
TTGTTAGACA AAATCAGACA AACCAACGTT CAAGGTGGTG AAGCTGGTGG CATCACCCAA 
CAGATTGGTG CCACTTATTT CCCCATCGAC GCTATTAAGG CAAAAACTAA AGTTATGGCT 
GAATATGAAA AACAAACTTT TGATGTCCCA GGTCTTTTGG TTATTGATAC CCCAGGTCAC 
GAATCCTTCT CTAACTTACG TTCAAGAGGT TCTTCATTGT GTAACATCGC AATTTTGGTT 
ATTGACATTA TGCATGGTTT GGAACAACAG ACTATTGAAT CTATCAAACT GTTAAGAGAT 
AGAAAGGCTC CATTTGTCGT TGCCCTAAAC AAAATTGATA GATTATATGA CTGGAAAGCC 
ATTCCAAACA ATTCATTCAG AGACTCCTTT GCAAAGCAAT CAAGAGCTGT TCAAGAGGAA 
TTTCAATCTA GGTATTCTAA GATTCAATTG GAATTAGCTG AACAAGGTTT GAATTCGGAA 
TTGTATTTCC AAAACAAAAA TATGTCTAAG TATGTCTCCA TTGTCCCAAC ATCTGCCGTC 
ACCGGTGAGG GTGTTCCAGA TTTATTGTGG TTGCTATTAG AATTGACCCA AAAGAGGATG 
TCCAAACAAT TGATGTACTT GTCTCACGTG GAAGCAACCA TTTTGGAAGT GAAAGTCGTA 
GAAGGTTTTG GTACCACAAT TGATGTTATC TTGTCCAACG GTTACTTGAG AGAAGGTGAC 
CGTATTGTAC TGTGTGGTAT GAATGGTCCA ATTGTAACGA ATATCAGAGC ATTACTAACA 
CCACAACCAT TACGTGAACT ACGTTTGAAA TCTGAATATG TCCACCACAA AGAAGTCAAG 
GCTGCTTTAG GTGTCAAGAT TGCCGCTAAT GATTTAGAAA AAGCCGTTTC TGGTTCTAGG 
CTGCTAGTTG TCGGTCCTGA AGATGACGAA GATGAATTGA TGGACGACGT TATGGATGAT 
TTGACTGGTT TGTTGGACTC CGTTGACACA ACTGGTAAAG GTGTTGTGGT CCAAGCATCC 
ACCTTGGGTT CTTTGGAAGC TTTGTTGGAT TTCTTGAAAG ACATGAAAAT CCCTGTGATG 
TCTATCGGGT TAGGTCCAGT GTACAAGCGT GATGTTATGA AAGCCTCCAC TATGTTGGAA 
AAGGCTCCAG AGTATGCCGT GATGTTATGT TTTGATGTTA AAGTGGATAA GGAAGCTGAA 
CAATACGCTG AACAAGAAGG AATTAAGATC TTTAATGCAG ACGTCATCTA TCATTTATTT 
GATTCATTTA CAGCATACCA AGAAAAGTTA TTGGAAGAAC GTCGTAAAGA TTTCCTAGAT 
TACGCTATTT TCCCATGTGT CTTACAAACC TTACAAATTA TTAACAAACG TGGTCCAATG 
ATTATTGGTG TAGACGTTCT GGAAGGTACT CTACGTGTGG GAACTCCTAT TTGCGCTGTG 
AAAACCGACC CTACTACAAA GGAAAGACAA ACTTTGATAT TAGGTAAAGT CATCTCTTTA 
GAAATCAACC ATCAACCTGT CCAAGAAGTA AAGAAGGGCC AAACCGCTGC TGGTGTTGCC 
GTCCGTCTAG AAGATCCCTC CGGTCAACAA CCTATCTGGG GTCGTCATGT TGACGAGAAT 
GATACATTAT ACTCCTTGGT TTCAAGAAGA TCTATTGACA CTTTGAAGGA TAAAGCTTTT 
AGGGACCAAG TTGCTAGATC CGATTGGCTG CTATTGAAGA AGCTGAAGGT CGTTTTCGGC 
ATCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009365.1
CDS	1..3009
Translation	MAKKSKKNQQNYWDEEFEEDAAQNEEISATPTPNPESSAGADDTSREASASAEGAEAIEGDFMSTLKQSKKKQEKKVIEEKKDGKPILKSKKEKEKEKKEKEKQKKKEQAARKKAQQQAQKEKNKELNKQNVEKAAAEKAAAEKSQKSKGESDKPSASAKKPAKKVPAGLAALRRQLELKKQLEEQEKLEREEEERLEKEEEERLANEEKMKEEAKAAKKEKEKAKREKRKAEGKLLTRKQKEEKKLLERRRAALLSSGNVKVAGLAKKDGEENKPKKVVYSKKKKRTTQENASEAIKSDSKKDSEVVPDDELKESEDVLIDDWENLALGDDDEEGTNEETQESTASHENEDQNQGEEEEEGEEEEEEEEERAHVHEVAKSTPAATPAATPTPSSASPNKKDLRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPIDAIKAKTKVMAEYEKQTFDVPGLLVIDTPGHESFSNLRSRGSSLCNIAILVIDIMHGLEQQTIESIKLLRDRKAPFVVALNKIDRLYDWKAIPNNSFRDSFAKQSRAVQEEFQSRYSKIQLELAEQGLNSELYFQNKNMSKYVSIVPTSAVTGEGVPDLLWLLLELTQKRMSKQLMYLSHVEATILEVKVVEGFGTTIDVILSNGYLREGDRIVLCGMNGPIVTNIRALLTPQPLRELRLKSEYVHHKEVKAALGVKIAANDLEKAVSGSRLLVVGPEDDEDELMDDVMDDLTGLLDSVDTTGKGVVVQASTLGSLEALLDFLKDMKIPVMSIGLGPVYKRDVMKASTMLEKAPEYAVMLCFDVKVDKEAEQYAEQEGIKIFNADVIYHLFDSFTAYQEKLLEERRKDFLDYAIFPCVLQTLQIINKRGPMIIGVDVLEGTLRVGTPICAVKTDPTTKERQTLILGKVISLEINHQPVQEVKKGQTAAGVAVRLEDPSGQQPIWGRHVDENDTLYSLVSRRSIDTLKDKAFRDQVARSDWLLLKKLKVVFGIE

Target ORF information:

RefSeq Version	NM_001178180.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C translation initiation factor eIF5B (FUN12), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178180.1

ORF Insert Sequence:

ATGGCGAAAA AGAGTAAAAA GAACCAACAG AACTACTGGG ATGAGGAATT CGAAGAAGAC 
GCCGCCCAGA ACGAAGAAAT CAGTGCCACG CCAACTCCAA ATCCAGAAAG CAGCGCAGGT 
GCAGATGACA CTTCCAGAGA AGCAAGTGCA AGTGCTGAAG GTGCTGAGGC CATTGAAGGC 
GACTTCATGT CTACTTTGAA GCAATCGAAG AAGAAGCAAG AAAAGAAGGT TATTGAAGAG 
AAGAAGGATG GTAAGCCTAT ACTAAAGTCC AAGAAGGAAA AGGAGAAGGA AAAAAAGGAA 
AAGGAGAAGC AGAAGAAGAA AGAACAAGCT GCCAGGAAGA AGGCCCAACA GCAAGCTCAA 
AAGGAGAAGA ACAAGGAGTT GAACAAGCAA AATGTTGAAA AAGCTGCTGC TGAGAAGGCT 
GCTGCTGAGA AATCCCAAAA ATCTAAAGGT GAAAGTGATA AACCAAGTGC TAGTGCTAAG 
AAGCCAGCCA AGAAAGTACC TGCCGGTTTG GCTGCTTTGA GACGTCAATT AGAATTGAAG 
AAACAACTTG AAGAACAAGA AAAGTTGGAA AGAGAGGAAG AAGAAAGATT GGAGAAAGAA 
GAGGAGGAAA GATTGGCCAA CGAAGAAAAA ATGAAGGAAG AAGCTAAAGC AGCTAAAAAG 
GAAAAGGAGA AGGCAAAGCG TGAAAAACGA AAGGCTGAAG GTAAGCTATT GACCAGAAAG 
CAAAAAGAAG AAAAGAAATT ATTGGAAAGA AGACGTGCCG CTTTATTGTC TTCCGGTAAC 
GTCAAAGTTG CCGGTCTGGC CAAGAAGGAT GGAGAAGAAA ACAAACCAAA GAAGGTTGTT 
TACAGCAAGA AGAAGAAGAG AACAACCCAG GAAAACGCCT CCGAAGCCAT TAAATCTGAC 
TCTAAGAAAG ACTCGGAAGT TGTACCTGAT GACGAACTCA AAGAATCCGA AGATGTTTTG 
ATTGATGATT GGGAAAATTT GGCTCTTGGT GATGATGACG AGGAGGGAAC CAACGAAGAA 
ACGCAAGAAT CCACCGCAAG CCATGAAAAT GAAGACCAAA ATCAAGGCGA AGAAGAAGAA 
GAAGGAGAAG AAGAAGAAGA AGAAGAAGAA GAAAGAGCAC ATGTGCATGA AGTTGCCAAA 
AGCACACCAG CAGCTACACC AGCAGCTACT CCAACTCCAT CCAGCGCTTC TCCAAACAAA 
AAAGATCTTC GTTCCCCAAT TTGTTGTATT TTGGGTCATG TCGATACCGG TAAGACTAAA 
TTGTTAGACA AAATCAGACA AACCAACGTT CAAGGTGGTG AAGCTGGTGG CATCACCCAA 
CAGATTGGTG CCACTTATTT CCCCATCGAC GCTATTAAGG CAAAAACTAA AGTTATGGCT 
GAATATGAAA AACAAACTTT TGATGTCCCA GGTCTTTTGG TTATTGATAC CCCAGGTCAC 
GAATCCTTCT CTAACTTACG TTCAAGAGGT TCTTCATTGT GTAACATCGC AATTTTGGTT 
ATTGACATTA TGCATGGTTT GGAACAACAG ACTATTGAAT CTATCAAACT GTTAAGAGAT 
AGAAAGGCTC CATTTGTCGT TGCCCTAAAC AAAATTGATA GATTATATGA CTGGAAAGCC 
ATTCCAAACA ATTCATTCAG AGACTCCTTT GCAAAGCAAT CAAGAGCTGT TCAAGAGGAA 
TTTCAATCTA GGTATTCTAA GATTCAATTG GAATTAGCTG AACAAGGTTT GAATTCGGAA 
TTGTATTTCC AAAACAAAAA TATGTCTAAG TATGTCTCCA TTGTCCCAAC ATCTGCCGTC 
ACCGGTGAGG GTGTTCCAGA TTTATTGTGG TTGCTATTAG AATTGACCCA AAAGAGGATG 
TCCAAACAAT TGATGTACTT GTCTCACGTG GAAGCAACCA TTTTGGAAGT GAAAGTCGTA 
GAAGGTTTTG GTACCACAAT TGATGTTATC TTGTCCAACG GTTACTTGAG AGAAGGTGAC 
CGTATTGTAC TGTGTGGTAT GAATGGTCCA ATTGTAACGA ATATCAGAGC ATTACTAACA 
CCACAACCAT TACGTGAACT ACGTTTGAAA TCTGAATATG TCCACCACAA AGAAGTCAAG 
GCTGCTTTAG GTGTCAAGAT TGCCGCTAAT GATTTAGAAA AAGCCGTTTC TGGTTCTAGG 
CTGCTAGTTG TCGGTCCTGA AGATGACGAA GATGAATTGA TGGACGACGT TATGGATGAT 
TTGACTGGTT TGTTGGACTC CGTTGACACA ACTGGTAAAG GTGTTGTGGT CCAAGCATCC 
ACCTTGGGTT CTTTGGAAGC TTTGTTGGAT TTCTTGAAAG ACATGAAAAT CCCTGTGATG 
TCTATCGGGT TAGGTCCAGT GTACAAGCGT GATGTTATGA AAGCCTCCAC TATGTTGGAA 
AAGGCTCCAG AGTATGCCGT GATGTTATGT TTTGATGTTA AAGTGGATAA GGAAGCTGAA 
CAATACGCTG AACAAGAAGG AATTAAGATC TTTAATGCAG ACGTCATCTA TCATTTATTT 
GATTCATTTA CAGCATACCA AGAAAAGTTA TTGGAAGAAC GTCGTAAAGA TTTCCTAGAT 
TACGCTATTT TCCCATGTGT CTTACAAACC TTACAAATTA TTAACAAACG TGGTCCAATG 
ATTATTGGTG TAGACGTTCT GGAAGGTACT CTACGTGTGG GAACTCCTAT TTGCGCTGTG 
AAAACCGACC CTACTACAAA GGAAAGACAA ACTTTGATAT TAGGTAAAGT CATCTCTTTA 
GAAATCAACC ATCAACCTGT CCAAGAAGTA AAGAAGGGCC AAACCGCTGC TGGTGTTGCC 
GTCCGTCTAG AAGATCCCTC CGGTCAACAA CCTATCTGGG GTCGTCATGT TGACGAGAAT 
GATACATTAT ACTCCTTGGT TTCAAGAAGA TCTATTGACA CTTTGAAGGA TAAAGCTTTT 
AGGGACCAAG TTGCTAGATC CGATTGGCTG CTATTGAAGA AGCTGAAGGT CGTTTTCGGC 
ATCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.
Proceedings of the National Academy of Sciences of the United States of America92(9)3809-13(1995 Apr)
Bussey H,Kaback DB,Zhong W,Vo DT,Clark MW,Fortin N,Hall J,Ouellette BF,Keng T,Barton AB

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FUN12 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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