VAC14 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VAC14
Entrez Gene ID	851102
Full Name	Vac14p
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182275.3	NP_013490.3	Vac14p

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vac14	NP_808791.1
Canis lupus familiaris (dog)	VAC14	XP_546837.2
Bos taurus (cattle)	VAC14	NP_001075913.1
Danio rerio (zebrafish)	vac14	NP_001004650.1
Kluyveromyces lactis	KLLA0E05193g	XP_002999395.1
Eremothecium gossypii	AGOS_AER049W	NP_984909.2
Neurospora crassa	NCU02409	XP_959537.2
Oryza sativa (rice)	Os03g0223700	NP_001049425.1
Mus musculus (house mouse)	Vac14	NP_666328.2
Drosophila melanogaster (fruit fly)	CG5608	NP_650208.1
Arabidopsis thaliana (thale cress)	AT2G01690	NP_973395.1
Pan troglodytes (chimpanzee)	VAC14	XP_001170940.1
Gallus gallus (chicken)	VAC14	NP_001025735.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000770	XP_311309.5
Caenorhabditis elegans (roundworm)	K04G2.6	NP_492215.1
Schizosaccharomyces pombe (fission yeast)	SPBC25H2.03	NP_596363.1
Magnaporthe oryzae (rice blast fungus)	MGG_03568	XP_003716347.1
Homo sapiens (human)	VAC14	NP_060522.3
	VAC14	XP_001106962.1
Saccharomyces cerevisiae (baker's yeast)	VAC14	NP_013490.3
Xenopus tropicalis (tropical clawed frog)	vac14	NP_001135664.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and funct
Title: Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function.

The authors show that Vac14 exerts an effect as a scaffold for the PI(3,5)P2 regulatory complex by direct contact with the known regulators of PI(3,5)P2: Fig4, Fab1, Vac7 and Atg18.
Title: VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse.

Vac14p-Fig4p complex controls the hyperosmotic shock-induced increase in phosphatidylinositol 3,5-diphosphate levels.
Title: The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover.

The following VAC14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAC14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00701	NM_001182275.3 Latest version!	Saccharomyces cerevisiae S288C Vac14p (VAC14), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VAC14 ( NM_001182275.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013490.3 Saccharomyces cerevisiae S288C Vac14p (VAC14), partial mRNA.

Price & Availability↑

CloneID	OSi00701
Clone ID Related Accession (Same CDS sequence)	NM_001182275.3
Accession Version	NM_001182275.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2643bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Vac14p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182275.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAAAAAT CGATTGCCAA AGGTTTAAGC GACAAACTAT ACGAAAAACG GAAGGCTGCC 
GCCTTGGAGT TGGAAAAGTT GGTCAAACAA TGCGTTCTAG AAGGGGACTA TGATCGTATT 
GACAAGATAA TAGACGAACT ATGCAGAGAC TATGCATACG CTTTGCATCA GCCCATGGCT 
AGAAATGCAG GATTAATGGG CTTGGCTGCC ACAGCAATTG CTTTAGGAAT AAACGATGTT 
GGCCGTTACT TGAGGAATAT TCTTCCTCCA GTTCTCGCAT GTTTTGGCGA CCAGAATGAC 
CAAGTAAGAT TTTATGCGTG TGAGAGTTTG TACAATATTG CAAAGATTGC GAAGGGTGAA 
ATACTGGTCT ATTTCAATGA AATATTTGAC GTACTCTGTA AAATTAGTGC TGATACAGAA 
AACTCGGTCC GAGGAGCAGC TGAACTATTA GATCGGTTGA TAAAAGATAT TGTTGCTGAA 
AGAGCATCCA ATTACATAAG CATTGTAAAT AATGGATCGC ATGGTTTGCT TCCAGCCATA 
AAAACGGATC CAATCAGCGG GGATGTCTAC CAAGAAGAAT ATGAGCAAGA TAATCAATTG 
GCGTTCTCTT TACCAAAATT TATTCCTTTA TTAACAGAGA GAATTTACGC CATTAACCCT 
GATACGCGTG TTTTTTTAGT TGACTGGCTT AAAGTCTTGC TCAATACCCC AGGACTAGAG 
CTCATATCGT ATCTACCATC ATTTCTTGGC GGGTTATTCA CGTTCTTGGG CGATTCTCAT 
AAAGATGTCA GAACAGTTAC ACACACCCTT ATGGACTCAC TACTTCACGA AGTCGACCGC 
ATTTCAAAAT TACAAACTGA AATTAAAATG AAGAGACTGG AAAGACTGAA AATGCTGGAA 
GATAAATACA ATAACAGTTC TACTCCAACG AAAAAGGCCG ATGGTGCACT TATTGCTGAA 
AAGAAGAAAA CTTTAATGAC TGCATTAGGT GGGTTATCCA AACCTCTGAG TATGGAGACT 
GATGATACCA AATTATCTAA TACTAATGAG ACAGATGATG AAAGACACTT GACAAGCCAG 
GAGCAGCTGC TAGATAGCGA GGCAACTAGC CAAGAACCTC TTCGCGATGG CGAAGAATAT 
ATACCTGGTC AAGATATCAA CCTCAATTTC CCAGAGGTTA TTACCGTTCT TGTGAACAAT 
TTGGCATCAT CTGAGGCAGA GATTCAATTG ATTGCATTAC ACTGGATTCA GGTTATTTTA 
TCCATTTCTC CAAATGTGTT TATCCCCTTC CTTTCAAAAA TTCTTTCTGT GCTTTTAAAG 
CTATTGAGCG ATTCGGATCC GCATATTACT GAAATTGCTC AGCTGGTGAA CGGTCAATTA 
CTCTCATTGT GCAGCTCATA TGTGGGAAAA GAGACAGACG GGAAAATTGC ATACGGGCCA 
ATCGTTAATA GCTTAACTCT ACAGTTTTTC GATAGTAGGA TTGATGCCAA GATTGCCTGC 
TTGGATTGGC TCATCTTAAT TTATCACAAG GCTCCAAACC AAATCTTGAA ACATAACGAC 
AGCATGTTCC TAACTTTGTT GAAGTCATTA TCAAATAGAG ACTCGGTGCT AATCGAGAAA 
GCTTTAAGTC TCTTACAGAG TTTATGTTCT GACTCAAATG ATAATTATTT ACGTCAGTTT 
CTTCAAGATT TATTAACTTT ATTCAAGAGA GATACCAAAT TGGTGAAAAC TAGAGCAAAC 
TTTATCATGA GACAGATATC TTCCCGTTTA TCACCAGAAC GTGTTTATAA AGTAATTTCT 
TCGATATTGG ACAATTATAA CGACACAACG TTTGTGAAAA TGATGATTCA GATTTTAAGT 
ACAAACTTAA TAACTTCACC GGAGATGTCG TCGTTAAGAA ACAAACTTAG AACTTGTGAA 
GACGGTATGT TTTTCAATAG TTTATTCAAA TCTTGGTGTC CCAACCCCGT GTCCGTGATA 
TCCTTATGTT TTGTAGCAGA AAATTATGAG TTAGCTTACA CAGTGTTACA AACTTACGCG 
AACTACGAGT TGAAGTTAAA TGATCTAGTG CAGTTGGATA TTTTGATTCA GTTGTTCGAA 
TCACCCGTTT TCACAAGGAT GCGGTTGCAA CTTTTAGAAC AACAGAAACA TCCGTTTCTG 
CATAAGTGTC TATTTGGTAT ACTGATGATT ATACCACAGT CAAAGGCATT TGAAACATTG 
AACCGCAGAT TGAATAGTTT GAACATATGG ACATCCCAAT CTTATGTCAT GAACAACTAT 
ATAAGACAAA GGGAGAATAG TAACTTTTGT GATTCCAATT CTGATATCTC GCAAAGGTCC 
GTTAGCCAAA GTAAACTACA TTTCCAGGAA CTAATTAATC ATTTTAAGGC CGTTTCAGAA 
GAAGATGAAT ATTCATCAGA TATGATAAGG CTTGACCATG GTGCTAACAA TAAATCACTT 
CTGCTTGGAA GTTTTTTGGA CGGTATTGAC GAAGATAAAC AAGAAATTGT CACACCAATC 
TCGCCCATGA ATGAAGCTAT TAACGAGGAG ATGGAATCTC CCAACGATAA TAGTTCTGTC 
ATATTAAAAG ATAGTGGCAG TCTGCCATTC AACCGCAATG TATCCGATAA ATTAAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013490.3
CDS	1..2643
Translation	MEKSIAKGLSDKLYEKRKAAALELEKLVKQCVLEGDYDRIDKIIDELCRDYAYALHQPMARNAGLMGLAATAIALGINDVGRYLRNILPPVLACFGDQNDQVRFYACESLYNIAKIAKGEILVYFNEIFDVLCKISADTENSVRGAAELLDRLIKDIVAERASNYISIVNNGSHGLLPAIKTDPISGDVYQEEYEQDNQLAFSLPKFIPLLTERIYAINPDTRVFLVDWLKVLLNTPGLELISYLPSFLGGLFTFLGDSHKDVRTVTHTLMDSLLHEVDRISKLQTEIKMKRLERLKMLEDKYNNSSTPTKKADGALIAEKKKTLMTALGGLSKPLSMETDDTKLSNTNETDDERHLTSQEQLLDSEATSQEPLRDGEEYIPGQDINLNFPEVITVLVNNLASSEAEIQLIALHWIQVILSISPNVFIPFLSKILSVLLKLLSDSDPHITEIAQLVNGQLLSLCSSYVGKETDGKIAYGPIVNSLTLQFFDSRIDAKIACLDWLILIYHKAPNQILKHNDSMFLTLLKSLSNRDSVLIEKALSLLQSLCSDSNDNYLRQFLQDLLTLFKRDTKLVKTRANFIMRQISSRLSPERVYKVISSILDNYNDTTFVKMMIQILSTNLITSPEMSSLRNKLRTCEDGMFFNSLFKSWCPNPVSVISLCFVAENYELAYTVLQTYANYELKLNDLVQLDILIQLFESPVFTRMRLQLLEQQKHPFLHKCLFGILMIIPQSKAFETLNRRLNSLNIWTSQSYVMNNYIRQRENSNFCDSNSDISQRSVSQSKLHFQELINHFKAVSEEDEYSSDMIRLDHGANNKSLLLGSFLDGIDEDKQEIVTPISPMNEAINEEMESPNDNSSVILKDSGSLPFNRNVSDKLKK

Target ORF information:

RefSeq Version	NM_001182275.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Vac14p (VAC14), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182275.3

ORF Insert Sequence:

ATGGAAAAAT CGATTGCCAA AGGTTTAAGC GACAAACTAT ACGAAAAACG GAAGGCTGCC 
GCCTTGGAGT TGGAAAAGTT GGTCAAACAA TGCGTTCTAG AAGGGGACTA TGATCGTATT 
GACAAGATAA TAGACGAACT ATGCAGAGAC TATGCATACG CTTTGCATCA GCCCATGGCT 
AGAAATGCAG GATTAATGGG CTTGGCTGCC ACAGCAATTG CTTTAGGAAT AAACGATGTT 
GGCCGTTACT TGAGGAATAT TCTTCCTCCA GTTCTCGCAT GTTTTGGCGA CCAGAATGAC 
CAAGTAAGAT TTTATGCGTG TGAGAGTTTG TACAATATTG CAAAGATTGC GAAGGGTGAA 
ATACTGGTCT ATTTCAATGA AATATTTGAC GTACTCTGTA AAATTAGTGC TGATACAGAA 
AACTCGGTCC GAGGAGCAGC TGAACTATTA GATCGGTTGA TAAAAGATAT TGTTGCTGAA 
AGAGCATCCA ATTACATAAG CATTGTAAAT AATGGATCGC ATGGTTTGCT TCCAGCCATA 
AAAACGGATC CAATCAGCGG GGATGTCTAC CAAGAAGAAT ATGAGCAAGA TAATCAATTG 
GCGTTCTCTT TACCAAAATT TATTCCTTTA TTAACAGAGA GAATTTACGC CATTAACCCT 
GATACGCGTG TTTTTTTAGT TGACTGGCTT AAAGTCTTGC TCAATACCCC AGGACTAGAG 
CTCATATCGT ATCTACCATC ATTTCTTGGC GGGTTATTCA CGTTCTTGGG CGATTCTCAT 
AAAGATGTCA GAACAGTTAC ACACACCCTT ATGGACTCAC TACTTCACGA AGTCGACCGC 
ATTTCAAAAT TACAAACTGA AATTAAAATG AAGAGACTGG AAAGACTGAA AATGCTGGAA 
GATAAATACA ATAACAGTTC TACTCCAACG AAAAAGGCCG ATGGTGCACT TATTGCTGAA 
AAGAAGAAAA CTTTAATGAC TGCATTAGGT GGGTTATCCA AACCTCTGAG TATGGAGACT 
GATGATACCA AATTATCTAA TACTAATGAG ACAGATGATG AAAGACACTT GACAAGCCAG 
GAGCAGCTGC TAGATAGCGA GGCAACTAGC CAAGAACCTC TTCGCGATGG CGAAGAATAT 
ATACCTGGTC AAGATATCAA CCTCAATTTC CCAGAGGTTA TTACCGTTCT TGTGAACAAT 
TTGGCATCAT CTGAGGCAGA GATTCAATTG ATTGCATTAC ACTGGATTCA GGTTATTTTA 
TCCATTTCTC CAAATGTGTT TATCCCCTTC CTTTCAAAAA TTCTTTCTGT GCTTTTAAAG 
CTATTGAGCG ATTCGGATCC GCATATTACT GAAATTGCTC AGCTGGTGAA CGGTCAATTA 
CTCTCATTGT GCAGCTCATA TGTGGGAAAA GAGACAGACG GGAAAATTGC ATACGGGCCA 
ATCGTTAATA GCTTAACTCT ACAGTTTTTC GATAGTAGGA TTGATGCCAA GATTGCCTGC 
TTGGATTGGC TCATCTTAAT TTATCACAAG GCTCCAAACC AAATCTTGAA ACATAACGAC 
AGCATGTTCC TAACTTTGTT GAAGTCATTA TCAAATAGAG ACTCGGTGCT AATCGAGAAA 
GCTTTAAGTC TCTTACAGAG TTTATGTTCT GACTCAAATG ATAATTATTT ACGTCAGTTT 
CTTCAAGATT TATTAACTTT ATTCAAGAGA GATACCAAAT TGGTGAAAAC TAGAGCAAAC 
TTTATCATGA GACAGATATC TTCCCGTTTA TCACCAGAAC GTGTTTATAA AGTAATTTCT 
TCGATATTGG ACAATTATAA CGACACAACG TTTGTGAAAA TGATGATTCA GATTTTAAGT 
ACAAACTTAA TAACTTCACC GGAGATGTCG TCGTTAAGAA ACAAACTTAG AACTTGTGAA 
GACGGTATGT TTTTCAATAG TTTATTCAAA TCTTGGTGTC CCAACCCCGT GTCCGTGATA 
TCCTTATGTT TTGTAGCAGA AAATTATGAG TTAGCTTACA CAGTGTTACA AACTTACGCG 
AACTACGAGT TGAAGTTAAA TGATCTAGTG CAGTTGGATA TTTTGATTCA GTTGTTCGAA 
TCACCCGTTT TCACAAGGAT GCGGTTGCAA CTTTTAGAAC AACAGAAACA TCCGTTTCTG 
CATAAGTGTC TATTTGGTAT ACTGATGATT ATACCACAGT CAAAGGCATT TGAAACATTG 
AACCGCAGAT TGAATAGTTT GAACATATGG ACATCCCAAT CTTATGTCAT GAACAACTAT 
ATAAGACAAA GGGAGAATAG TAACTTTTGT GATTCCAATT CTGATATCTC GCAAAGGTCC 
GTTAGCCAAA GTAAACTACA TTTCCAGGAA CTAATTAATC ATTTTAAGGC CGTTTCAGAA 
GAAGATGAAT ATTCATCAGA TATGATAAGG CTTGACCATG GTGCTAACAA TAAATCACTT 
CTGCTTGGAA GTTTTTTGGA CGGTATTGAC GAAGATAAAC AAGAAATTGT CACACCAATC 
TCGCCCATGA ATGAAGCTAT TAACGAGGAG ATGGAATCTC CCAACGATAA TAGTTCTGTC 
ATATTAAAAG ATAGTGGCAG TCTGCCATTC AACCGCAATG TATCCGATAA ATTAAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VAC14 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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