VPS34 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS34
Entrez Gene ID	850941
Full Name	phosphatidylinositol 3-kinase VPS34
Synonyms	END12,PEP15,STT8,VPL7,VPS7,VPT29
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182127.1	NP_013341.1	phosphatidylinositol 3-kinase VPS34

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PIK3C3	XP_003642950.2
Caenorhabditis elegans (roundworm)	vps-34	NP_001020954.1
Saccharomyces cerevisiae (baker's yeast)	VPS34	NP_013341.1
Kluyveromyces lactis	KLLA0D18645g	XP_453887.1
Eremothecium gossypii	AGOS_AGL113C	NP_986554.1
Arabidopsis thaliana (thale cress)	VPS34	NP_176251.1
Pan troglodytes (chimpanzee)	PIK3C3	XP_001142812.1
Mus musculus (house mouse)	Pik3c3	NP_852079.2
Drosophila melanogaster (fruit fly)	Pi3K59F	NP_477133.1
Schizosaccharomyces pombe (fission yeast)	pik3	NP_594699.1
Xenopus tropicalis (tropical clawed frog)	pik3c3	XP_002935097.2
Canis lupus familiaris (dog)	PIK3C3	XP_547599.3
Homo sapiens (human)	PIK3C3	NP_002638.2
Magnaporthe oryzae (rice blast fungus)	MGG_03069	XP_003720606.1
Oryza sativa (rice)	Os05g0180600	NP_001054810.1
	PIK3C3	XP_001082892.1
Bos taurus (cattle)	PIK3C3	NP_001092537.1
Rattus norvegicus (Norway rat)	Pik3c3	NP_075247.1
Danio rerio (zebrafish)	pik3c3	NP_001017550.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010273	XP_319464.4
Neurospora crassa	NCU00656	XP_965796.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that class Ia Phosphatidylinositol 3-Kinase p85alpha germ line mutations associated with SHORT syndrome did not increase PI3K activity in the yeast model.
Title: Insights into the pathological mechanisms of p85? mutations using a yeast-based phosphatidylinositol 3-kinase model.

mutants lacking Vps34 display multiple phenotypes indicating impaired transcription elongation.
Title: Vps factors are required for efficient transcription elongation in budding yeast.

VPS34 plays a critical role in the decline of extracellular FBPase in response to glucose.
Title: Vps34p is required for the decline of extracellular fructose-1,6-bisphosphatase in the vacuole import and degradation pathway.

Kes1-mediated regulation of the autophagy/cytoplasm to the vacuole trafficking pathway is prevented by increasing expression of the PI3K Vps34
Title: Lipid binding requirements for oxysterol-binding protein Kes1 inhibition of autophagy and endosome-trans-Golgi trafficking pathways.

Study shows that the lipid-kinase activity of Vps34 is required for autophagy, implying an essential role of its product PtdIns(3)P.
Title: Transport of phosphatidylinositol 3-phosphate into the vacuole via autophagic membranes in Saccharomyces cerevisiae.

The following VPS34 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS34 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00573	NM_001182127.1 Latest version!	Saccharomyces cerevisiae S288C phosphatidylinositol 3-kinase VPS34 (VPS34), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS34 ( NM_001182127.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013341.1 Saccharomyces cerevisiae S288C phosphatidylinositol 3-kinase VPS34 (VPS34), partial mRNA.

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CloneID	OSi00573
Clone ID Related Accession (Same CDS sequence)	NM_001182127.1
Accession Version	NM_001182127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	phosphatidylinositol 3-kinase VPS34
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCACTGA ACAACATAAC ATTCTGTGTC TCACAGGATC TGGATGTTCC CCTGAAAGTG 
AAAATCAAGT CATTGGAAGG ACATAAGCCA CTGTTGAAGC CATCTCAAAA AATCCTGAAC 
CCTGAATTAA TGCTGATAGG GTCAAATGTA TTCCCTTCTA GTGATCTAAT TGTATCCTTA 
CAGGTATTTG ATAAAGAGAG AAACAGAAAT TTAACTCTTC CGATATACAC GCCGTATATT 
CCTTTCAGAA ACTCTAGAAC CTGGGATTAT TGGTTGACTT TGCCCATACG TATCAAACAG 
CTTACCTTTA GCAGTCATTT ACGCATTATT TTGTGGGAAT ACAATGGATC CAAGCAAATT 
CCCTTTTTCA ATCTAGAAAC GAGCATCTTT AACTTAAAAG ACTGTACTTT AAAAAGAGGG 
TTTGAATCCT TAAAATTTCG TTATGATGTG ATTGACCACT GTGAAGTGGT CACTGATAAC 
AAAGATCAAG AAAATTTGAA TAAATATTTT CAGGGTGAAT TCACGAGACT CCCTTGGCTT 
GACGAAATCA CTATAAGCAA ATTAAGGAAA CAACGGGAAA ATAGGACTTG GCCTCAGGGC 
ACCTTTGTCT TAAACTTAGA ATTTCCAATG TTAGAGCTTC CTGTTGTGTT CATCGAAAGG 
GAAATTATGA ATACTCAAAT GAATATTCCA ACTTTGAAGA ACAATCCTGG ACTAAGCACA 
GACTTACGAG AACCGAATAG GAATGATCCT CAAATCAAAA TTTCTTTGGG GGACAAATAT 
CACTCCACAT TGAAGTTTTA CGATCCTGAC CAACCAAACA ATGATCCAAT AGAGGAAAAG 
TATAGGAGAT TGGAAAGAGC ATCTAAAAAT GCAAACTTGG ACAAGCAAGT GAAGCCGGAC 
ATTAAAAAGA GGGACTACTT GAATAAAATC ATCAACTACC CCCCTGGCAC TAAATTAACA 
GCACATGAAA AGGGTTCAAT ATGGAAATAT AGGTATTATT TGATGAACAA TAAAAAAGCT 
CTTACAAAGT TATTACAGAG TACAAATTTG AGAGAAGAAT CGGAAAGAGT AGAGGTTTTA 
GAACTAATGG ACTCATGGGC AGAAATTGAT ATAGACGACG CATTAGAACT ATTAGGCTCA 
ACTTTTAAAA ACCTTTCAGT GAGATCGTAC GCCGTAAATA GGCTGAAGAA GGCATCAGAC 
AAGGAGCTGG AATTGTACTT ATTACAGTTG GTAGAAGCTG TCTGTTTTGA GAACCTGTCC 
ACTTTTTCCG ACAAATCTAA CAGTGAATTC ACTATTGTGG ATGCCGTATC TTCGCAAAAG 
CTTTCGGGTG ATTCTATGTT ACTATCTACA TCGCATGCCA ACCAAAAATT ATTAAAGTCC 
ATCTCGAGTG AATCGGAAAC TTCCGGGACA GAATCGCTAC CAATCGTCAT TTCTCCCTTG 
GCTGAGTTTT TGATTAGGAG AGCACTGGTC AACCCAAGGT TAGGAAGTTT TTTCTATTGG 
TATTTAAAGT CCGAGTCTGA GGACAAGCCA TATTTAGACC AGATTTTAAG TTCCTTTTGG 
AGTAGACTGG ATAAAAAATC TCGGAATATA CTGAACGATC AAGTCAGGCT AATAAATGTG 
CTCAGGGAAT GTTGTGAAAC AATTAAGAGG CTGAAAGATA CTACAGCCAA AAAGATGGAA 
TTACTAGTGC ATTTATTGGA GACGAAAGTC AGGCCTCTTG TCAAAGTACG ACCAATTGCT 
TTACCACTAG ACCCTGATGT GTTGATATGC GACGTTTGTC CTGAAACTTC GAAGGTATTT 
AAAAGCTCTT TATCGCCACT AAAAATAACA TTCAAAACGA CCTTAAACCA ACCATATCAC 
TTAATGTTTA AGGTTGGCGA TGATTTGAGA CAAGATCAAC TGGTAGTACA GATCATAAGT 
TTAATGAATG AGTTATTAAA AAATGAAAAC GTGGACTTGA AATTGACACC ATACAAAATT 
TTGGCAACAG GACCACAAGA AGGTGCCATT GAATTTATCC CTAATGACAC ATTAGCTAGC 
ATACTAAGCA AATATCACGG TATTCTTGGC TACCTTAAAC TCCACTATCC TGATGAGAAC 
GCGACATTAG GCGTACAAGG CTGGGTTTTA GATAATTTTG TCAAATCTTG TGCTGGCTAT 
TGTGTTATTA CATACATCTT AGGTGTCGGC GATAGGCATT TAGACAACTT ACTAGTCACG 
CCAGATGGGC ACTTTTTTCA CGCAGACTTT GGTTATATCT TGGGTCAGGA CCCCAAACCT 
TTTCCGCCAT TAATGAAATT GCCCCCTCAA ATTATAGAGG CGTTTGGGGG TGCAGAATCA 
TCAAATTATG ATAAATTTCG CAGCTACTGT TTTGTGGCAT ATTCGATTTT AAGAAGGAAT 
GCAGGCTTAA TCTTAAACCT GTTTGAATTA ATGAAGACTT CGAACATACC TGATATCAGA 
ATAGATCCCA ATGGTGCTAT ATTACGTGTA AGAGAGAGAT TTAATTTGAA TATGTCCGAA 
GAAGATGCCA CAGTGCATTT TCAGAATCTA ATCAATGATA GTGTAAATGC TTTGCTGCCT 
ATCGTGATTG ATCATTTACA TAATCTGGCA CAATACTGGC GGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013341.1
CDS	1..2628
Translation	MSLNNITFCVSQDLDVPLKVKIKSLEGHKPLLKPSQKILNPELMLIGSNVFPSSDLIVSLQVFDKERNRNLTLPIYTPYIPFRNSRTWDYWLTLPIRIKQLTFSSHLRIILWEYNGSKQIPFFNLETSIFNLKDCTLKRGFESLKFRYDVIDHCEVVTDNKDQENLNKYFQGEFTRLPWLDEITISKLRKQRENRTWPQGTFVLNLEFPMLELPVVFIEREIMNTQMNIPTLKNNPGLSTDLREPNRNDPQIKISLGDKYHSTLKFYDPDQPNNDPIEEKYRRLERASKNANLDKQVKPDIKKRDYLNKIINYPPGTKLTAHEKGSIWKYRYYLMNNKKALTKLLQSTNLREESERVEVLELMDSWAEIDIDDALELLGSTFKNLSVRSYAVNRLKKASDKELELYLLQLVEAVCFENLSTFSDKSNSEFTIVDAVSSQKLSGDSMLLSTSHANQKLLKSISSESETSGTESLPIVISPLAEFLIRRALVNPRLGSFFYWYLKSESEDKPYLDQILSSFWSRLDKKSRNILNDQVRLINVLRECCETIKRLKDTTAKKMELLVHLLETKVRPLVKVRPIALPLDPDVLICDVCPETSKVFKSSLSPLKITFKTTLNQPYHLMFKVGDDLRQDQLVVQIISLMNELLKNENVDLKLTPYKILATGPQEGAIEFIPNDTLASILSKYHGILGYLKLHYPDENATLGVQGWVLDNFVKSCAGYCVITYILGVGDRHLDNLLVTPDGHFFHADFGYILGQDPKPFPPLMKLPPQIIEAFGGAESSNYDKFRSYCFVAYSILRRNAGLILNLFELMKTSNIPDIRIDPNGAILRVRERFNLNMSEEDATVHFQNLINDSVNALLPIVIDHLHNLAQYWRT

Target ORF information:

RefSeq Version	NM_001182127.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C phosphatidylinositol 3-kinase VPS34 (VPS34), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182127.1

ORF Insert Sequence:

ATGTCACTGA ACAACATAAC ATTCTGTGTC TCACAGGATC TGGATGTTCC CCTGAAAGTG 
AAAATCAAGT CATTGGAAGG ACATAAGCCA CTGTTGAAGC CATCTCAAAA AATCCTGAAC 
CCTGAATTAA TGCTGATAGG GTCAAATGTA TTCCCTTCTA GTGATCTAAT TGTATCCTTA 
CAGGTATTTG ATAAAGAGAG AAACAGAAAT TTAACTCTTC CGATATACAC GCCGTATATT 
CCTTTCAGAA ACTCTAGAAC CTGGGATTAT TGGTTGACTT TGCCCATACG TATCAAACAG 
CTTACCTTTA GCAGTCATTT ACGCATTATT TTGTGGGAAT ACAATGGATC CAAGCAAATT 
CCCTTTTTCA ATCTAGAAAC GAGCATCTTT AACTTAAAAG ACTGTACTTT AAAAAGAGGG 
TTTGAATCCT TAAAATTTCG TTATGATGTG ATTGACCACT GTGAAGTGGT CACTGATAAC 
AAAGATCAAG AAAATTTGAA TAAATATTTT CAGGGTGAAT TCACGAGACT CCCTTGGCTT 
GACGAAATCA CTATAAGCAA ATTAAGGAAA CAACGGGAAA ATAGGACTTG GCCTCAGGGC 
ACCTTTGTCT TAAACTTAGA ATTTCCAATG TTAGAGCTTC CTGTTGTGTT CATCGAAAGG 
GAAATTATGA ATACTCAAAT GAATATTCCA ACTTTGAAGA ACAATCCTGG ACTAAGCACA 
GACTTACGAG AACCGAATAG GAATGATCCT CAAATCAAAA TTTCTTTGGG GGACAAATAT 
CACTCCACAT TGAAGTTTTA CGATCCTGAC CAACCAAACA ATGATCCAAT AGAGGAAAAG 
TATAGGAGAT TGGAAAGAGC ATCTAAAAAT GCAAACTTGG ACAAGCAAGT GAAGCCGGAC 
ATTAAAAAGA GGGACTACTT GAATAAAATC ATCAACTACC CCCCTGGCAC TAAATTAACA 
GCACATGAAA AGGGTTCAAT ATGGAAATAT AGGTATTATT TGATGAACAA TAAAAAAGCT 
CTTACAAAGT TATTACAGAG TACAAATTTG AGAGAAGAAT CGGAAAGAGT AGAGGTTTTA 
GAACTAATGG ACTCATGGGC AGAAATTGAT ATAGACGACG CATTAGAACT ATTAGGCTCA 
ACTTTTAAAA ACCTTTCAGT GAGATCGTAC GCCGTAAATA GGCTGAAGAA GGCATCAGAC 
AAGGAGCTGG AATTGTACTT ATTACAGTTG GTAGAAGCTG TCTGTTTTGA GAACCTGTCC 
ACTTTTTCCG ACAAATCTAA CAGTGAATTC ACTATTGTGG ATGCCGTATC TTCGCAAAAG 
CTTTCGGGTG ATTCTATGTT ACTATCTACA TCGCATGCCA ACCAAAAATT ATTAAAGTCC 
ATCTCGAGTG AATCGGAAAC TTCCGGGACA GAATCGCTAC CAATCGTCAT TTCTCCCTTG 
GCTGAGTTTT TGATTAGGAG AGCACTGGTC AACCCAAGGT TAGGAAGTTT TTTCTATTGG 
TATTTAAAGT CCGAGTCTGA GGACAAGCCA TATTTAGACC AGATTTTAAG TTCCTTTTGG 
AGTAGACTGG ATAAAAAATC TCGGAATATA CTGAACGATC AAGTCAGGCT AATAAATGTG 
CTCAGGGAAT GTTGTGAAAC AATTAAGAGG CTGAAAGATA CTACAGCCAA AAAGATGGAA 
TTACTAGTGC ATTTATTGGA GACGAAAGTC AGGCCTCTTG TCAAAGTACG ACCAATTGCT 
TTACCACTAG ACCCTGATGT GTTGATATGC GACGTTTGTC CTGAAACTTC GAAGGTATTT 
AAAAGCTCTT TATCGCCACT AAAAATAACA TTCAAAACGA CCTTAAACCA ACCATATCAC 
TTAATGTTTA AGGTTGGCGA TGATTTGAGA CAAGATCAAC TGGTAGTACA GATCATAAGT 
TTAATGAATG AGTTATTAAA AAATGAAAAC GTGGACTTGA AATTGACACC ATACAAAATT 
TTGGCAACAG GACCACAAGA AGGTGCCATT GAATTTATCC CTAATGACAC ATTAGCTAGC 
ATACTAAGCA AATATCACGG TATTCTTGGC TACCTTAAAC TCCACTATCC TGATGAGAAC 
GCGACATTAG GCGTACAAGG CTGGGTTTTA GATAATTTTG TCAAATCTTG TGCTGGCTAT 
TGTGTTATTA CATACATCTT AGGTGTCGGC GATAGGCATT TAGACAACTT ACTAGTCACG 
CCAGATGGGC ACTTTTTTCA CGCAGACTTT GGTTATATCT TGGGTCAGGA CCCCAAACCT 
TTTCCGCCAT TAATGAAATT GCCCCCTCAA ATTATAGAGG CGTTTGGGGG TGCAGAATCA 
TCAAATTATG ATAAATTTCG CAGCTACTGT TTTGTGGCAT ATTCGATTTT AAGAAGGAAT 
GCAGGCTTAA TCTTAAACCT GTTTGAATTA ATGAAGACTT CGAACATACC TGATATCAGA 
ATAGATCCCA ATGGTGCTAT ATTACGTGTA AGAGAGAGAT TTAATTTGAA TATGTCCGAA 
GAAGATGCCA CAGTGCATTT TCAGAATCTA ATCAATGATA GTGTAAATGC TTTGCTGCCT 
ATCGTGATTG ATCATTTACA TAATCTGGCA CAATACTGGC GGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VPS34 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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