SNF7 cDNA ORF clone, Saccharomyces cerevisiae S288C

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Novel mutations has been characterized in ESCRT-III Snf7 that trigger activation, and a novel role have been assigned to Bro1, the yeast ALIX ortholog, in Snf7 assembly.
Title: ESCRT-III activation by parallel action of ESCRT-I/II and ESCRT-0/Bro1 during MVB biogenesis.

The major ESCRT-III subunit Snf7 localizes transiently to vesicles and tubules surrounded by a cup-shaped membrane and this was accelerated in vps4Delta cells, correlating with increased Acb1 secretion.
Title: ESCRT-III drives the final stages of CUPS maturation for unconventional protein secretion.

Using a combination of biochemical and genetic approaches, both in vitro and in vivo, we demonstrate that mutations on these protein interfaces halt Snf7 assembly and block endosomal sorting complexes required for transport function.
Title: Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments.

Binding of Vps4 to ESCRT-III, coordinated by interactions with Vps2 and Snf7, is coupled to membrane neck constriction during intraluminal vesicle formation.
Title: Coordinated binding of Vps4 to ESCRT-III drives membrane neck constriction during MVB vesicle formation.

we demonstrate that the N terminus of Snf7 has been tuned to maintain the topological constraints associated with ESCRT-III-filament-mediated membrane invagination and vesicle formation.
Title: Essential N-terminal insertion motif anchors the ESCRT-III filament during MVB vesicle formation.