MET10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MET10
Entrez Gene ID	850588
Full Name	sulfite reductase subunit alpha
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179995.3	NP_116686.3	sulfite reductase subunit alpha

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPCC584.01c	NP_588222.1
Magnaporthe oryzae (rice blast fungus)	MGG_00929	XP_003718023.1
Kluyveromyces lactis	KLLA0D15983g	XP_453764.1
Eremothecium gossypii	AGOS_AGR237C	NP_986903.1
Saccharomyces cerevisiae (baker's yeast)	MET10	NP_116686.3
Neurospora crassa	NCU04077	XP_957692.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus, a compromised MsrA function combined with alpha-synuclein overexpression may promote processes leading to synucleinopathies.
Title: Clearance and phosphorylation of alpha-synuclein are inhibited in methionine sulfoxide reductase a null yeast cells.

Specific mutations in the MET10 and MET5 genes, which encode the catalytic alpha- and beta-subunits of the sulfite reductase enzyme, respectively, were identified in strains producing decreased amount of H2S.
Title: Isolation of sulfite reductase variants of a commercial wine yeast with significantly reduced hydrogen sulfide production.

The following MET10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MET10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00266	NM_001179995.3 Latest version!	Saccharomyces cerevisiae S288C sulfite reductase subunit alpha (MET10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MET10 ( NM_001179995.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116686.3 Saccharomyces cerevisiae S288C sulfite reductase subunit alpha (MET10), partial mRNA.

Price & Availability↑

CloneID	OSi00266
Clone ID Related Accession (Same CDS sequence)	NM_001179995.3
Accession Version	NM_001179995.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	sulfite reductase subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). On Jul 30, 2012 this sequence version replaced NM_001179995.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCAGTTG AGTTTGCTAC CAATCCTTTT GGCGAGGCCA AAAATGCAAC TTCACTGCCA 
AAATATGGTA CACCCGTAAC TGCCATTTCA TCTGTGCTGT TCAATAACGT GGACTCCATT 
TTTGCTTACA AGTCCTTTTC TCAACCCGAT TTGCTACACC AAGATCTAAA AAAATGGTCT 
GAAAAGCGTG GTAACGAATC ACGTGGGAAG CCATTTTTCC AAGAGCTGGA TATCAGATCT 
GGCGCTGGTT TGGCTCCTTT AGGGTTTTCT CATGGATTGA AGAACACTAC AGCAATTGTT 
GCTCCAGGGT TTTCGCTGCC ATACTTCATT AACTCTTTGA AAACCGTCTC TCATGATGGT 
AAGTTTCTTT TGAATGTTGG TGCTTTAAAC TACGACAATG CTACCGGCTC TGTCACCAAC 
GATTATGTAA CCGCATTGGA TGCTGCTTCC AAGCTGAAGT ATGGTGTCGT GACTCCGATT 
TCCGCTAACG AGGTACAAAG TGTCGCCTTA CTGGCATTGG CGATTGCCAC TTTCAGTAAT 
AACTCCGGAG CTATCAATTT ATTTGACGGA TTAAACTACT CGAAAACCGT CTTGCCGTTG 
GTCGAATCTG TTCCTGAGGC ATCTATTTTG GCAAAACTAT CCAAAGTTAT TGCACCAGAT 
GCTGCCTTTG ATGATGTCTT GGATAAGTTT AATGAATTGA CTGGATTGAG ACTACATAAT 
TTCCAATACT TTGGTGCTCA GGATGCTGAA ACTGTGTTTA TCACTTATGG GTCTTTAGAA 
TCCGAATTGT TCAACTCTGC GATTAGTGGT AATAATTCCA AAATCGGGTT AATCAACGTC 
AGAGTGCCAT TACCTTTTAA CGTTGCTAAG TTTGTCACTC ACGTTCCATC CACTACCAAA 
CAAATTGTTG TTATAGGCCA AACTTTGGAT GGTTCTTCGC CTTCTTTCTT GAGATCTCAA 
GTTTCAGCCG CCTTATTTTA CCACGGCCGC ACCTCAATTA GCGTTTCTGA GTACATCTAT 
CAACCAGATT TCATTTGGTC CCCAAAAGCT GTCAAATCAA TTGTATCGTC ATTCATCCCT 
GAATTCACTT ACAATGCCGA TTCATCTTTC GGCGAAGGAT TCATTTATTG GGCCTCTGAT 
AAGAGTATCA ATATTGATGT TGCCTCCAAA CTTGTGAAAG CTCTGTCTTT GGAAGATGGG 
AAATTTGTGT CTTTGAGAAC GAAATTTGAT AACTTGGCTA ATGCTGGTAC CTTCCAAGCT 
CAATTTGTGA CCTCGAAAGA ACAGATACCT GTTTCAAACA TCGATTCTAC GAAATTATCA 
GTCGTTGAAG ATGTCAGTTT ATTGAAGCAT TTAGACGTAG CTGCTACCGT CGCAGAACAA 
GGTTCAATTG CGTTGGTTTC CCAAAAGGCA GTTAAAGATT TGGATTTAAA TTCTGTAGAA 
AGTTACGTCA AGAATTTGGG AATTCCTGAA TCATTCCTAA TATCTATTGC GAAGAAAAAC 
ATCAAATTGT TTATCATCGA TGGTGAGACC ACTAACGACG AGTCCAAATT GTCCTTGTTT 
ATCCAAGCCG TTTTCTGGAA ATTGGCCTTC GGTCTAGATG TCGCAGAATG TACCAACCGT 
ATCTGGAAAA GCATTGATTC AGGTGCAGAC ATTTCAGCAG CCTCGATTTC TGAATTTCTC 
ACTGGTGCAT TCAAAAACTT CCTCAGTGAG GTTCCGCTAG CGCTGTACAC TAAATTTTCT 
GAAATAAACA TTGAAAAGAA AGAGGATGAG GAAGAGCCTG CAGCTTTACC AATTTTCGTT 
AATGAAACAT CTTTCCTCCC AAATAACAGT ACCATTGAAG AAATACCATT ACCTGAGACC 
TCTGAGATCT CTGATATTGC CAAGAAGTTG TCCTTCAAAG AGGCATATGA AGTTGAGAAT 
AAACTAAGAC CCGATTTACC CGTCAAGAAC TTCGTCGTGA AAGTTAAAGA AAATAGACGT 
GTTACGCCTG CTGATTATGA TAGATATATT TTCCATATTG AATTCGATAT TTCTGGTACT 
GGAATGACTT ATGACATCGG TGAAGCCCTC GGTATTCATG CCAGAAACAA TGAATCTTTG 
GTCAAAGAAT TCTTAACCTT CTATGGTCTA AATGAATCCG ATGTTGTCTT AGTCCCCAAC 
AAGGACAACC ACCATTTGTT AGAAACAAGA ACCGTCTTAC AAGCATTTGT GGAAAATTTG 
GATATTTTCG GTAAACCACC AAAAAGATTT TACGAATCAT TGATTCCATA TGCCTCTAAC 
GAAGAGGAGA AGAAAAAATT AGAGGATTTG GTTACTCCTG CCGGTGCAGT AGATTTGAAG 
AGATTTCAAG ATGTGGAGTA TTATACATAT GCTGACATTT TTGAATTGTT CCCATCTGTT 
CGCCCATCTC TTGAGGAACT TGTTACTATC ATTGAACCAT TGAAGAGAAG AGAATACTCA 
ATTGCCTCCT CTCAGAAAGT TCATCCAAAT GAAGTTCATT TATTGATCGT TGTTGTTGAT 
TGGGTGGATA ATAAAGGAAG AAAAAGGTAC GGTCAAGCTT CTAAGTATAT CTCAGACCTT 
GCTGTCGGTT CAGAATTGGT CGTTAGCGTT AAACCATCTG TTATGAAATT ACCACCATCT 
CCAAAGCAAC CAGTTATTAT GAGTGGTTTA GGTACTGGTT TGGCACCATT CAAGGCCATT 
GTTGAAGAGA AATTATGGCA AAAGCAGCAA GGTTATGAGA TTGGTGAAGT CTTCCTATAT 
CTAGGTTCAA GACACAAAAG AGAAGAATAT TTATATGGTG AGTTATGGGA GGCTTACAAA 
GATGCAGGTA TTATCACACA CATCGGCGCT GCTTTCTCAA GAGACCAACC TCAAAAAATT 
TACATTCAAG ATCGTATCAA AGAGAATTTG GATGAATTAA AAACTGCAAT GATTGATAAT 
AAAGGTTCAT TTTACTTGTG TGGCCCTACT TGGCCAGTTC CAGATATTAC TCAAGCTTTG 
CAAGACATTC TGGCTAAAGA CGCCGAGGAA AGAGGCATCA AAGTCGACTT GGATGCCGCA 
ATTGAAGAAT TAAAGGAAGC ATCAAGATAC ATTTTAGAAG TCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116686.3
CDS	1..3108
Translation	MPVEFATNPFGEAKNATSLPKYGTPVTAISSVLFNNVDSIFAYKSFSQPDLLHQDLKKWSEKRGNESRGKPFFQELDIRSGAGLAPLGFSHGLKNTTAIVAPGFSLPYFINSLKTVSHDGKFLLNVGALNYDNATGSVTNDYVTALDAASKLKYGVVTPISANEVQSVALLALAIATFSNNSGAINLFDGLNYSKTVLPLVESVPEASILAKLSKVIAPDAAFDDVLDKFNELTGLRLHNFQYFGAQDAETVFITYGSLESELFNSAISGNNSKIGLINVRVPLPFNVAKFVTHVPSTTKQIVVIGQTLDGSSPSFLRSQVSAALFYHGRTSISVSEYIYQPDFIWSPKAVKSIVSSFIPEFTYNADSSFGEGFIYWASDKSINIDVASKLVKALSLEDGKFVSLRTKFDNLANAGTFQAQFVTSKEQIPVSNIDSTKLSVVEDVSLLKHLDVAATVAEQGSIALVSQKAVKDLDLNSVESYVKNLGIPESFLISIAKKNIKLFIIDGETTNDESKLSLFIQAVFWKLAFGLDVAECTNRIWKSIDSGADISAASISEFLTGAFKNFLSEVPLALYTKFSEINIEKKEDEEEPAALPIFVNETSFLPNNSTIEEIPLPETSEISDIAKKLSFKEAYEVENKLRPDLPVKNFVVKVKENRRVTPADYDRYIFHIEFDISGTGMTYDIGEALGIHARNNESLVKEFLTFYGLNESDVVLVPNKDNHHLLETRTVLQAFVENLDIFGKPPKRFYESLIPYASNEEEKKKLEDLVTPAGAVDLKRFQDVEYYTYADIFELFPSVRPSLEELVTIIEPLKRREYSIASSQKVHPNEVHLLIVVVDWVDNKGRKRYGQASKYISDLAVGSELVVSVKPSVMKLPPSPKQPVIMSGLGTGLAPFKAIVEEKLWQKQQGYEIGEVFLYLGSRHKREEYLYGELWEAYKDAGIITHIGAAFSRDQPQKIYIQDRIKENLDELKTAMIDNKGSFYLCGPTWPVPDITQALQDILAKDAEERGIKVDLDAAIEELKEASRYILEVY

Target ORF information:

RefSeq Version	NM_001179995.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C sulfite reductase subunit alpha (MET10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179995.3

ORF Insert Sequence:

ATGCCAGTTG AGTTTGCTAC CAATCCTTTT GGCGAGGCCA AAAATGCAAC TTCACTGCCA 
AAATATGGTA CACCCGTAAC TGCCATTTCA TCTGTGCTGT TCAATAACGT GGACTCCATT 
TTTGCTTACA AGTCCTTTTC TCAACCCGAT TTGCTACACC AAGATCTAAA AAAATGGTCT 
GAAAAGCGTG GTAACGAATC ACGTGGGAAG CCATTTTTCC AAGAGCTGGA TATCAGATCT 
GGCGCTGGTT TGGCTCCTTT AGGGTTTTCT CATGGATTGA AGAACACTAC AGCAATTGTT 
GCTCCAGGGT TTTCGCTGCC ATACTTCATT AACTCTTTGA AAACCGTCTC TCATGATGGT 
AAGTTTCTTT TGAATGTTGG TGCTTTAAAC TACGACAATG CTACCGGCTC TGTCACCAAC 
GATTATGTAA CCGCATTGGA TGCTGCTTCC AAGCTGAAGT ATGGTGTCGT GACTCCGATT 
TCCGCTAACG AGGTACAAAG TGTCGCCTTA CTGGCATTGG CGATTGCCAC TTTCAGTAAT 
AACTCCGGAG CTATCAATTT ATTTGACGGA TTAAACTACT CGAAAACCGT CTTGCCGTTG 
GTCGAATCTG TTCCTGAGGC ATCTATTTTG GCAAAACTAT CCAAAGTTAT TGCACCAGAT 
GCTGCCTTTG ATGATGTCTT GGATAAGTTT AATGAATTGA CTGGATTGAG ACTACATAAT 
TTCCAATACT TTGGTGCTCA GGATGCTGAA ACTGTGTTTA TCACTTATGG GTCTTTAGAA 
TCCGAATTGT TCAACTCTGC GATTAGTGGT AATAATTCCA AAATCGGGTT AATCAACGTC 
AGAGTGCCAT TACCTTTTAA CGTTGCTAAG TTTGTCACTC ACGTTCCATC CACTACCAAA 
CAAATTGTTG TTATAGGCCA AACTTTGGAT GGTTCTTCGC CTTCTTTCTT GAGATCTCAA 
GTTTCAGCCG CCTTATTTTA CCACGGCCGC ACCTCAATTA GCGTTTCTGA GTACATCTAT 
CAACCAGATT TCATTTGGTC CCCAAAAGCT GTCAAATCAA TTGTATCGTC ATTCATCCCT 
GAATTCACTT ACAATGCCGA TTCATCTTTC GGCGAAGGAT TCATTTATTG GGCCTCTGAT 
AAGAGTATCA ATATTGATGT TGCCTCCAAA CTTGTGAAAG CTCTGTCTTT GGAAGATGGG 
AAATTTGTGT CTTTGAGAAC GAAATTTGAT AACTTGGCTA ATGCTGGTAC CTTCCAAGCT 
CAATTTGTGA CCTCGAAAGA ACAGATACCT GTTTCAAACA TCGATTCTAC GAAATTATCA 
GTCGTTGAAG ATGTCAGTTT ATTGAAGCAT TTAGACGTAG CTGCTACCGT CGCAGAACAA 
GGTTCAATTG CGTTGGTTTC CCAAAAGGCA GTTAAAGATT TGGATTTAAA TTCTGTAGAA 
AGTTACGTCA AGAATTTGGG AATTCCTGAA TCATTCCTAA TATCTATTGC GAAGAAAAAC 
ATCAAATTGT TTATCATCGA TGGTGAGACC ACTAACGACG AGTCCAAATT GTCCTTGTTT 
ATCCAAGCCG TTTTCTGGAA ATTGGCCTTC GGTCTAGATG TCGCAGAATG TACCAACCGT 
ATCTGGAAAA GCATTGATTC AGGTGCAGAC ATTTCAGCAG CCTCGATTTC TGAATTTCTC 
ACTGGTGCAT TCAAAAACTT CCTCAGTGAG GTTCCGCTAG CGCTGTACAC TAAATTTTCT 
GAAATAAACA TTGAAAAGAA AGAGGATGAG GAAGAGCCTG CAGCTTTACC AATTTTCGTT 
AATGAAACAT CTTTCCTCCC AAATAACAGT ACCATTGAAG AAATACCATT ACCTGAGACC 
TCTGAGATCT CTGATATTGC CAAGAAGTTG TCCTTCAAAG AGGCATATGA AGTTGAGAAT 
AAACTAAGAC CCGATTTACC CGTCAAGAAC TTCGTCGTGA AAGTTAAAGA AAATAGACGT 
GTTACGCCTG CTGATTATGA TAGATATATT TTCCATATTG AATTCGATAT TTCTGGTACT 
GGAATGACTT ATGACATCGG TGAAGCCCTC GGTATTCATG CCAGAAACAA TGAATCTTTG 
GTCAAAGAAT TCTTAACCTT CTATGGTCTA AATGAATCCG ATGTTGTCTT AGTCCCCAAC 
AAGGACAACC ACCATTTGTT AGAAACAAGA ACCGTCTTAC AAGCATTTGT GGAAAATTTG 
GATATTTTCG GTAAACCACC AAAAAGATTT TACGAATCAT TGATTCCATA TGCCTCTAAC 
GAAGAGGAGA AGAAAAAATT AGAGGATTTG GTTACTCCTG CCGGTGCAGT AGATTTGAAG 
AGATTTCAAG ATGTGGAGTA TTATACATAT GCTGACATTT TTGAATTGTT CCCATCTGTT 
CGCCCATCTC TTGAGGAACT TGTTACTATC ATTGAACCAT TGAAGAGAAG AGAATACTCA 
ATTGCCTCCT CTCAGAAAGT TCATCCAAAT GAAGTTCATT TATTGATCGT TGTTGTTGAT 
TGGGTGGATA ATAAAGGAAG AAAAAGGTAC GGTCAAGCTT CTAAGTATAT CTCAGACCTT 
GCTGTCGGTT CAGAATTGGT CGTTAGCGTT AAACCATCTG TTATGAAATT ACCACCATCT 
CCAAAGCAAC CAGTTATTAT GAGTGGTTTA GGTACTGGTT TGGCACCATT CAAGGCCATT 
GTTGAAGAGA AATTATGGCA AAAGCAGCAA GGTTATGAGA TTGGTGAAGT CTTCCTATAT 
CTAGGTTCAA GACACAAAAG AGAAGAATAT TTATATGGTG AGTTATGGGA GGCTTACAAA 
GATGCAGGTA TTATCACACA CATCGGCGCT GCTTTCTCAA GAGACCAACC TCAAAAAATT 
TACATTCAAG ATCGTATCAA AGAGAATTTG GATGAATTAA AAACTGCAAT GATTGATAAT 
AAAGGTTCAT TTTACTTGTG TGGCCCTACT TGGCCAGTTC CAGATATTAC TCAAGCTTTG 
CAAGACATTC TGGCTAAAGA CGCCGAGGAA AGAGGCATCA AAGTCGACTT GGATGCCGCA 
ATTGAAGAAT TAAAGGAAGC ATCAAGATAC ATTTTAGAAG TCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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MET10 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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